Characterization of the protein processing and secretion pathways in a comprehensive set of expressed sequence tags from Trichoderma reesei.

abstract：:Pseudomonas syringae pv. tomato DC3000, a plant pathogenic gram-negative bacterium, employs the type III secretion system (T3SS) to cause disease in tomato and Arabidopsis and to induce the hypersensitive response in nonhost plants. The expression of T3SS is regulated by the HrpL extracytoplasmic sigma factor. Express...

journal_title：FEMS microbiology letters

authors： Jovanovic M,Lawton E,Schumacher J,Buck M

更新日期：2014-07-01 00:00:00

abstract：:We have previously reported that mycolyl glycolipids from Nocardia rubra such as glucose or trehalose mycolates induced granuloma formation in mice. The structure of the carbohydrate moiety of the mycolyl glycolipids influenced the granuloma forming activity profoundly. Here, we have examined the macrophage-chemotacti...

journal_title：FEMS microbiology letters

authors： Matsunaga I,Oka S,Inoue T,Yano I

更新日期：1990-01-15 00:00:00

abstract：:Lactobacillus johnsonii La1 (La1) is a probiotic strain capable of stimulating the immune system of the host and interfering with gastrointestinal pathogens. This study evaluates how the ingestion of different amounts of La1 influences the main bacterial populations of the fecal microbiota. Eight asymptomatic voluntee...

journal_title：FEMS microbiology letters

authors： Garrido D,Suau A,Pochart P,Cruchet S,Gotteland M

更新日期：2005-07-15 00:00:00

abstract：:The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The -35 and -10 hexamers correspond to a sigma 70-type promoter. This promoter responds to heat shock and involves an inverted repeat different from t...

journal_title：FEMS microbiology letters

authors： Brans A,Loriaux A,Thamm I,Joris B,Dusart J

更新日期：1997-04-15 00:00:00

abstract：:The photosynthetic bacterium Rhodobacter sulfidophilus is able to grow chemotrophically and phototrophically at a broad range of light intensities. In contrast to other facultative phototrophs, R. sulfidophilus synthesizes reaction center and light-harvesting (LH) complexes, B870 (LHI) and B800-850 (LHII) even under f...

journal_title：FEMS microbiology letters

authors： Hagemann GE,Gad'on N,Garcia A,Drews G,Tadros MH

更新日期：1995-02-01 00:00:00

abstract：:We report here improvements to the growth media and fermentation conditions which result in a substantial increase of Helicobacter pylori growth and in the enhanced production of vacuolating cytotoxin. Addition of glucose to the medium resulted in the increase of cell yield, cell viability and a significant improvemen...

journal_title：FEMS microbiology letters

authors： Marchini A,Massari P,Manetti R,Olivieri R

更新日期：1994-11-15 00:00:00

abstract：:A real-time nucleic acid sequence-based amplification assay, targeting tmRNA, was designed for the rapid identification of Staphylococcus aureus. The selectivity of the assay was confirmed against a panel of 76 Staphylococcus strains and species and 22 other bacterial species. A detection limit of 1 cell equivalent wa...

journal_title：FEMS microbiology letters

authors： O'Grady J,Lacey K,Glynn B,Smith TJ,Barry T,Maher M

更新日期：2009-12-01 00:00:00

abstract：:In Bacillus subtilis, utilisation of xylose, arabinose and ribose is controlled by the transcriptional factors XylR, AraR and RbsR, respectively. Here we apply the comparative approach to the analysis of these regulons in the Bacillus/Clostridium group. Evolutionary variability of operon structures is demonstrated and...

journal_title：FEMS microbiology letters

authors： Rodionov DA,Mironov AA,Gelfand MS

更新日期：2001-12-18 00:00:00

abstract：:Inoculation of soil with bacteria (a Gram-negative rod [PD2] and a 4-membered consortium [DC1]) accelerated mineralisation of phenanthrene and pyrene (but not naphthalene) added individually to a pristine sand and a pristine organic soil. The half-life of naphthalene was 3.5 days in both soils whether inoculated or no...

journal_title：FEMS microbiology letters

authors： Smith MJ,Lethbridge G,Burns RG

更新日期：1997-07-01 00:00:00

abstract：:Twelve strains of Candida albicans were grown in defined medium which had been deferrated by ion-exchange chromatography and then supplemented with FeCl3 to give iron concentrations ranging from 0.026 microM (growth-limiting) to 0.8 microM (excess). All of the strains secreted hydroxamate-type siderophores; phenolate ...

journal_title：FEMS microbiology letters

authors： Sweet SP,Douglas LJ

更新日期：1991-05-01 00:00:00

abstract：:The porin-associated protein of Rhodospirillum rubrum FR1 was found to contain a peptidoglycan binding motif. A partial fragment of 179 amino acids, obtained by cleavage of PAP with trypsin, Asp-N protease, and CNBr, was sequenced. Substantial sequence homology was found of the C-terminal part (residues 126-179) of po...

journal_title：FEMS microbiology letters

authors： Neumann U,Schiltz E,Stahl B,Hillenkamp F,Weckesser J

更新日期：1996-04-15 00:00:00

abstract：:Endoglucanase of Aspergillus niger AS-101 was partially purified by ammonium sulphate fractionation and molecular sieving on Sephadex G-200. The enzyme was found to be unstable on storage, however, it received some protection on the addition of BSA, glycerol or sodium azide. Glycerol also protected the enzyme from ina...

journal_title：FEMS microbiology letters

authors： Singh A,Agrawal AK,Abidi AB,Darmwal NS

更新日期：1990-09-01 00:00:00

abstract：:DNA polymerase from the archaeon Pyrococcus abyssi strain Orsay was expressed in Escherichia coli. The recombinant DNA polymerase (Pab) was purified to homogeneity by heat treatment followed by 5 steps of chromatography and characterized for PCR applications. Buffer optimization experiments indicated that Pab PCR perf...

journal_title：FEMS microbiology letters

authors： Dietrich J,Schmitt P,Zieger M,Preve B,Rolland JL,Chaabihi H,Gueguen Y

更新日期：2002-11-19 00:00:00

abstract：:The accumulation of 2-keto-3-deoxy-6-phosphogluconate, the key intermediate of the Entner-Doudoroff pathway, has long been thought to inhibit growth of bacteria, but careful measurements of 2-keto-3-deoxy-6-phosphogluconate accumulation by growing cells and the correlation of intracellular 2-keto-3-deoxy-6-phosphogluc...

journal_title：FEMS microbiology letters

authors： Fuhrman LK,Wanken A,Nickerson KW,Conway T

更新日期：1998-02-15 00:00:00

abstract：:Immunoassays are increasingly used to investigate the production, properties and fates of the cyanobacterial hepatotoxic microcystins in vitro and in vivo. Responses of an ELISA immunoassay to microcystins have been determined using the authentic toxin antigen, microcystin-LR, and conjugation products between the toxi...

journal_title：FEMS microbiology letters

authors： Metcalf JS,Beattie KA,Pflugmacher S,Codd GA

更新日期：2000-08-15 00:00:00

abstract：:The 42- and 51-kDa protein genes of Bacillus sphaericus 1593 have been subcloned independently downstream from the cytA gene promoter of Bacillus thuringiensis serovar israelensis and introduced into a non-mosquitocidal strain of Bacillus thuringiensis. Consequently, each protein was overproduced and accumulated as in...

journal_title：FEMS microbiology letters

authors： Nicolas L,Nielsen-Leroux C,Charles JF,Delécluse A

更新日期：1993-02-01 00:00:00

abstract：:Two different nucleic acid precursor utilization patterns were obtained for five avian isolates of Chlamydia psittaci. Three of the isolates behaved in a manner similar to that previously described, showing total dependency on the host cell for ribonucleoside triphosphates and being unable to utilize medium-supplied t...

journal_title：FEMS microbiology letters

authors： McClarty G,Fan H,Andersen AA

更新日期：1993-04-15 00:00:00

abstract：:Atypical psychrophilic Aeromonas salmonicida isolates were obtained from farmed and wild fish in Northeastern North America. These bacteria were isolated between 1992 and 2001 and carried tetracycline resistance (Tc(r)) plasmids of approximately 58 kb. The nine isolates had plasmids which could be divided into four gr...

journal_title：FEMS microbiology letters

authors： Casas C,Anderson EC,Ojo KK,Keith I,Whelan D,Rainnie D,Roberts MC

更新日期：2005-01-01 00:00:00

abstract：:The number and incidence of Collinsella aerofaciens in the human intestine are the highest among Gram-positive non-spore-forming bacilli. Identification of this species is very difficult and requires considerable time. A PCR-based identification system using C. aerofaciens-specific primers is described. Using this PCR...

journal_title：FEMS microbiology letters

authors： Kageyama A,Sakamoto M,Benno Y

更新日期：2000-02-01 00:00:00

abstract：:Tachyzoites of Toxoplasma gondii multiply within the parasitophorous vacuole (PV) until the lysis of the host cell. This study was undertaken to evaluate the effect of hydroxyurea (a specific drug that arrests cell division at G1/S phase) on the multiplication of T. gondii tachyzoites in infected Vero cells. Infected ...

journal_title：FEMS microbiology letters

authors： de Melo EJ,Mayerhoffer RO,de Souza W

更新日期：2000-04-01 00:00:00

abstract：:Contrary to its effect on rich medium, D-cycloserine showed no bactericidal effect on Zymomonas mobilis cells cultured on mineral medium. Addition of a mixture of glycine and glutamic acid to the mineral medium restored its bactericidal action. However, mutant enrichments run in these conditions were biased, with most...

journal_title：FEMS microbiology letters

authors： Pencreac'h G,Aït-Abdelkader N,Joset F,Baratti JC

更新日期：1996-09-01 00:00:00

abstract：:To determine the existence of an acid stress response in Helicobacter pylori the global changes in the proteins synthesized by the bacterium when subjected to an acid stress were studied. H. pylori ATCC43504 previously adapted to pH 7 did not show an acid stress response as detected by the two-dimensional electrophore...

journal_title：FEMS microbiology letters

authors： Toledo H,Valenzuela M,Rivas A,Jerez CA

更新日期：2002-07-16 00:00:00

abstract：:Plant protection via disease-suppressive bacteria in desert farming requires specific biological control agents (BCAs) adapted to the unique arid conditions. We performed an ecological study of below-ground communities in desert farm soil and untreated desert soil, and based on these findings, selected antagonists wer...

journal_title：FEMS microbiology letters

authors： Köberl M,Ramadan EM,Adam M,Cardinale M,Hallmann J,Heuer H,Smalla K,Berg G

更新日期：2013-05-01 00:00:00

abstract：:A cloning cassette, carrying a modified amino-glycoside resistance gene (neo) from transposon Tn5 was constructed. Three restriction sites internal to the neo gene were eliminated by in vitro mutagenesis, allowing their use in designing new cloning vectors. The original, suboptimal transcription promoter was replaced ...

journal_title：FEMS microbiology letters

authors： Denis F,Brzezinski R

更新日期：1991-07-01 00:00:00

abstract：:An iterative position-specific score matrix (PSSM)-based approach was used to predict sigma(28) promoters in 11 Shewanella genomes. The Shewanella Correlation Browser was used to distinguish true-positive predictions from false-positive predictions in Shewanella oneidensis MR-1 by generating a sigma(28)-regulated tran...

journal_title：FEMS microbiology letters

authors： Song W,Juhn FS,Naiman DQ,Konstantinidis KT,Gardner TS,Ward MJ

更新日期：2008-06-01 00:00:00

abstract：:A large cellulosomal gene cluster was identified in the recently sequenced genome of Clostridium acetobutylicum ATCC 824. Sequence analysis revealed that this cluster contains the genes for the scaffolding protein CipA, the processive endocellulase Cel48A, several endoglucanases of families 5 and 9, the mannanase Man5...

journal_title：FEMS microbiology letters

authors： Sabathé F,Bélaïch A,Soucaille P

更新日期：2002-11-19 00:00:00

abstract：:A composite transposon is a mobile genetic element consisting of two insertion sequences (ISs) flanking a segment of cargo DNA often containing antibiotic resistance (AR) genes. Composite transposons can move as a discreet unit. There have been recently several reports on a novel mechanism of movement of an IS26-based...

journal_title：FEMS microbiology letters

authors： Tansirichaiya S,Mullany P,Roberts AP

更新日期：2016-09-01 00:00:00

abstract：:Polyclonal antibodies were prepared against the major antenna chlorophyll (Chl) a/b-binding protein from the prokaryote Prochlorothrix hollandica (Burger-Wiersma et al. (1986) Nature (Lond.) 320, 262-264). Immunoblotting experiments on Triton X-114 phase-partitioned P. hollandica thylakoids revealed that the antibody ...

journal_title：FEMS microbiology letters

authors： Bullerjahn GS,Jensen TC,Sherman DM,Sherman LA

更新日期：1990-01-15 00:00:00

abstract：:We found the presence of DNA sequence which shows sequence similarity to the class IV chitin synthase gene (CHS3) of Saccharomyces cerevisiae in the genome of 14 Rhizopus species which belong to zygomycetes. We cloned a gene (chs3), which might correspond to one of these homologous sequences, from Rhizopus oligosporus...

journal_title：FEMS microbiology letters

authors： Motoyama T,Horiuchi H,Ohta A,Yamaguchi I,Takagi M

更新日期：1998-12-01 00:00:00

abstract：:The symbiotic plasmid (pSym) DNA present in bacteroids of strain RCR1001 of Rhizobium leguminosarum biovar viceae has been compared qualitatively and quantitatively with that present in free living bacteria by hybridization experiments with appropriate probes. A decrease in the relative amount of pSym DNA was observed...

journal_title：FEMS microbiology letters

authors： Vierny C,Iaccarino M

更新日期：1989-07-01 00:00:00