Respective role of the 42- and 51-kDa components of the Bacillus sphaericus toxin overexpressed in Bacillus thuringiensis.

Abstract:

:The 42- and 51-kDa protein genes of Bacillus sphaericus 1593 have been subcloned independently downstream from the cytA gene promoter of Bacillus thuringiensis serovar israelensis and introduced into a non-mosquitocidal strain of Bacillus thuringiensis. Consequently, each protein was overproduced and accumulated as inclusion bodies which were purified. For the first time, the 42-kDa protein inclusions alone were found to be toxic to Culex pipiens larvae (LC50 at 48 h 300 ng ml-1); in contrast, the 51-kDa protein inclusions were not. Moreover, a synergistic effect between these two components was observed.

journal_name

FEMS Microbiol Lett

authors

Nicolas L,Nielsen-Leroux C,Charles JF,Delécluse A

doi

10.1111/j.1574-6968.1993.tb05976.x

subject

Has Abstract

pub_date

1993-02-01 00:00:00

pages

275-80

issue

3

eissn

0378-1097

issn

1574-6968

journal_volume

106

pub_type

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