Abstract:
:There are two major pathways for methionine biosynthesis: the enterobacterial type transsulfuration pathway and the sulfhydrylation pathway as previously identified in the spirochete Leptospira meyeri. Sequence analysis of the L. meyeri metYX locus allows the identification of a third gene, called metW, which encodes a protein exhibiting similarities with homologs in many organisms belonging to the alpha-, beta-, and gamma-subdivisions of proteobacteria. The metW, metX and metY genes of L. meyeri were disrupted by a resistance cassette by homologous recombination. While the L. meyeri metX mutant shows methionine auxotrophy, the metY mutant (as well as the metW and metYmetW mutants) conserves methionine prototrophy, suggesting the presence of additional route(s) which may bypass the direct sulfhydrylation pathway. In addition, a L. interrogans gene, called metZ, was found to complement an Escherichia coli metB mutant, further suggesting that the transsulfuration pathway is also present in Leptospira spp.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Picardeau M,Bauby H,Saint Girons Idoi
10.1016/S0378-1097(03)00529-9keywords:
subject
Has Abstractpub_date
2003-08-29 00:00:00pages
257-62issue
2eissn
0378-1097issn
1574-6968pii
S0378109703005299journal_volume
225pub_type
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