Genetic evidence for the existence of two pathways for the biosynthesis of methionine in the Leptospira spp.

Abstract:

:There are two major pathways for methionine biosynthesis: the enterobacterial type transsulfuration pathway and the sulfhydrylation pathway as previously identified in the spirochete Leptospira meyeri. Sequence analysis of the L. meyeri metYX locus allows the identification of a third gene, called metW, which encodes a protein exhibiting similarities with homologs in many organisms belonging to the alpha-, beta-, and gamma-subdivisions of proteobacteria. The metW, metX and metY genes of L. meyeri were disrupted by a resistance cassette by homologous recombination. While the L. meyeri metX mutant shows methionine auxotrophy, the metY mutant (as well as the metW and metYmetW mutants) conserves methionine prototrophy, suggesting the presence of additional route(s) which may bypass the direct sulfhydrylation pathway. In addition, a L. interrogans gene, called metZ, was found to complement an Escherichia coli metB mutant, further suggesting that the transsulfuration pathway is also present in Leptospira spp.

journal_name

FEMS Microbiol Lett

authors

Picardeau M,Bauby H,Saint Girons I

doi

10.1016/S0378-1097(03)00529-9

keywords:

subject

Has Abstract

pub_date

2003-08-29 00:00:00

pages

257-62

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378109703005299

journal_volume

225

pub_type

杂志文章
  • Diversity of CTX-M beta-lactamases and their promoter regions from Enterobacteriaceae isolated in three Parisian hospitals.

    abstract::Nine clinical isolates of Enterobacteriaceae (six Escherichia coli and three Proteus mirabilis) isolated in three Parisian hospitals between 1989 and 2000 showed a particular extended-spectrum cephalosporin-resistance profile characterized by resistance to cefotaxime and aztreonam but not to ceftazidime. CTX-M-1, CTX-...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11126.x

    authors: Saladin M,Cao VT,Lambert T,Donay JL,Herrmann JL,Ould-Hocine Z,Verdet C,Delisle F,Philippon A,Arlet G

    更新日期:2002-04-09 00:00:00

  • Cloning of xylanase gene of Streptomyces flavogriseus in Escherichia coli and bacteriophage lambda-induced lysis for the release of cloned enzyme.

    abstract::The xylanase gene of Streptomyces flavogriseus was cloned in pUC8 plasmid and expressed in Escherichia coli lysogenic for lambda cI857. lambda-Induced lysis of E. coli at 42 degrees C allowed efficient release of cloned enzyme activity in extracellular environment. The xylanase gene was located in the 0.8-kb HindIII f...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(91)90158-7

    authors: Srivastava R,Ali SS,Srivastava BS

    更新日期:1991-03-01 00:00:00

  • Evidence for inter- and intraspecies biofilm formation variability among a small group of coagulase-negative staphylococci.

    abstract::Coagulase-negative staphylococci (CoNS) are common bacterial colonizers of the human skin. They are often involved in nosocomial infections due to biofilm formation in indwelling medical devices. While biofilm formation has been extensively studied in Staphylococcus epidermidis, little is known regarding other CoNS sp...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv175

    authors: Oliveira F,Lima CA,Brás S,França Â,Cerca N

    更新日期:2015-10-01 00:00:00

  • Collagen-induced STAT family members activation in Entamoeba histolytica trophozoites.

    abstract::The interaction of Entamoeba histolytica trophozoites with collagen type I and calcium induces a membrane to nuclei signaling. The transduction pathways involved in such phenomena are still poorly understood. Using a combination of immunoprecipitation assays, Western immunoblot analysis, electrophoretic mobility shift...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00822-X

    authors: Cruz-Vera J,Clara L,Hernández-Kelly R,Alfredo Méndez J,Pérez-Salazar E,Ortega A

    更新日期:2003-12-12 00:00:00

  • Arginine inhibits Na-driven flagellar motors of alkaliphilic Bacillus.

    abstract::L-arginine has attracted a great deal of attention as an agent for refolding denatured proteins, and the mildness of its effects offer hope for a wide range of potential applications for this substance, including medicines with few side effects. We report that both L- and D-arginine inhibits Na+-driven flagellar motor...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00117.x

    authors: Atsumi T,Yoshimura F,Sugiyama S

    更新日期:2006-03-01 00:00:00

  • Pathogenesis of Vibrio vulnificus.

    abstract::This review describes the factors which are currently recognized as being central to the virulence of the human pathogen, Vibrio vulnificus. This estuarine/marine bacterium occurs in high numbers in molluscan shellfish, primarily oysters, and its ingestion in raw oysters results in a ca. 60% mortality in those persons...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6968.1999.tb13570.x

    authors: Linkous DA,Oliver JD

    更新日期:1999-05-15 00:00:00

  • Isolation and identification of nematode-antagonistic compounds from the fungus Aspergillus candidus.

    abstract::Culture medium from an isolate of the fungus Aspergillus candidus was extracted, fractionated and examined to discover compounds antagonistic to plant-parasitic nematodes that are important pathogens of agricultural crops. Column, thin layer and preparative chromatographies and spectral and elemental analyses, were us...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnw026

    authors: Shemshura ON,Bekmakhanova NE,Mazunina MN,Meyer SL,Rice CP,Masler EP

    更新日期:2016-03-01 00:00:00

  • Physicochemical properties of Pseudomonas fragi isolates response to modified atmosphere packaging.

    abstract::Pseudomonas spp., in particular Pseudomonas fragi, are dominant in aerobically stored chilled meats. This work isolated P. fragi isolates from spoiled chicken and investigated the effect of modified atmosphere packaging (MAP, CO2/N2 30%/70%) on physicochemical properties of P. fragi isolates compared to the correspond...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnx106

    authors: Wang GY,Li M,Ma F,Wang HH,Xu XL,Zhou GH

    更新日期:2017-06-15 00:00:00

  • Direct profiling of the yeast dynamics in wine fermentations.

    abstract::We present a method to directly characterize the yeast diversity present in wine fermentations by employing denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 26S ribosomal RNA (rRNA) genes. PCR-DGGE of a portion of the 26S rRNA gene was shown to distinguish most yeast genera a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09210.x

    authors: Cocolin L,Bisson LF,Mills DA

    更新日期:2000-08-01 00:00:00

  • Corynebacterium diphtheriae surface proteins as adhesins to human erythrocytes.

    abstract::Corynebacterium diphtheriae strains express non-fimbrial surface proteins able to recognize and bind to specific host cells receptors. Protein extracts were obtained from bacterial cells by mechanical process and ammonium sulfate precipitation at 25 and 45% (w/v) saturation. SDS-PAGE analysis of the extracts detected ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10609.x

    authors: Colombo AV,Hirata R Jr,de Souza CM,Monteiro-Leal LH,Previato JO,Formiga LC,Andrade AF,Mattos-Guaraldi AL

    更新日期:2001-04-13 00:00:00

  • Morphological and intracellular alterations induced by cytotoxin VT2y produced by Escherichia coli isolated from chickens with swollen head syndrome.

    abstract::Recently, a novel verocytotoxin named VT2y was described which belongs to the STx family and is produced by Escherichia coli isolated from domestic poultry with swollen head syndrome (SHS). The VT2y toxin induced apoptosis in Vero, HeLa, CHO, CEF (primary chicken embryo fibroblast) and PCK (primary chicken kidney) cel...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10586.x

    authors: Salvadori MR,Yamada AT,Yano T

    更新日期:2001-04-01 00:00:00

  • Effects of forespore-specific overexpression of apurinic/apyrimidinic endonuclease Nfo on the DNA-damage resistance properties of Bacillus subtilis spores.

    abstract::The effects of overexpression of the apurinic/apyrimidinic DNA endonuclease Nfo on wet and dry heat and UV-C (254 nm) resistance of Bacillus subtilis spores with or without alpha/beta-type small, acid-soluble spore proteins (SASP) were determined. Results revealed that overexpression of Nfo > or =50-fold in spores inc...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01845.x

    authors: Barraza-Salas M,Ibarra-Rodríguez JR,Mellado SJ,Salas-Pacheco JM,Setlow P,Pedraza-Reyes M

    更新日期:2010-01-01 00:00:00

  • Development of a species-specific recA-based PCR test for Burkholderia fungorum.

    abstract::The genus Burkholderia comprises over 28 species and species-specific, recA-based polymerase chain reaction (PCR) tests are available for several species, but not for some soil-inhabiting species including B. fungorum. Previous analysis of several novel rhizospheric, environmental isolates belonging to the B. cepacia ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00443-9

    authors: Chan CH,Stead DE,Coutts RH

    更新日期:2003-07-15 00:00:00

  • Delineation of molecular species of a family of diacyltrehaloses from Mycobacterium fortuitum by mass spectrometry.

    abstract::A family of 2,3-di-O-acyl trehaloses (DAT), previously identified in Mycobacterium fortuitum, was studied by fast-atom bombardment mass spectrometry to establish the combinations of fatty acyl substituents and, hence, to delineate the molecular species there comprised. The mass spectra indicated the possible existence...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb06901.x

    authors: Ariza MA,Valero-Guillén PL

    更新日期:1994-06-15 00:00:00

  • Regulatory systems controlling motility and gene transfer agent production and release in Rhodobacter capsulatus.

    abstract::Production of the gene transfer agent of Rhodobacter capsulatus, RcGTA, is dependent upon several cellular regulatory systems, including a putative phosphorelay involving the CtrA and CckA proteins. These proteins are also involved in flagellar motility in R. capsulatus. The interactions of proteins in this system are...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2012.02553.x

    authors: Mercer RG,Quinlan M,Rose AR,Noll S,Beatty JT,Lang AS

    更新日期:2012-06-01 00:00:00

  • System-level modeling of acetone-butanol-ethanol fermentation.

    abstract::Acetone-butanol-ethanol (ABE) fermentation is a metabolic process of clostridia that produces bio-based solvents including butanol. It is enabled by an underlying metabolic reaction network and modulated by cellular gene regulation and environmental cues. Mathematical modeling has served as a valuable strategy to faci...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fnw074

    authors: Liao C,Seo SO,Lu T

    更新日期:2016-05-01 00:00:00

  • The isolation and characterisation of a Saccharomyces cerevisiae gene (LIP2) involved in the attachment of lipoic acid groups to mitochondrial enzymes.

    abstract::Lipoic acid is an essential cofactor for a variety of mitochondrial enzymes. We have characterised a gene from Saccharomyces cerevisiae which appears to encode a protein involved in the attachment of lipoic acid groups to the pyruvate dehydrogenase and glycine decarboxylase complexes. The predicted protein product of ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10663.x

    authors: Marvin ME,Williams PH,Cashmore AM

    更新日期:2001-05-15 00:00:00

  • Massive Open Online Courses: what will be their legacy?

    abstract::Massive Open Online Courses (MOOCs) dominated discussions of online learning and higher education in the news media and in universities between 2012 and 2015. However, fashions pass, needs change and technology evolves. This Commentary looks back, pauses on the present, and then looks forward. Whilst MOOCs are a signi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnw055

    authors: Daniel J

    更新日期:2016-04-01 00:00:00

  • Stable, temperature-sensitive recombinant strain of Mycobacterium smegmatis generated through the substitution of a psychrophilic ligA gene.

    abstract::A synthetic version of the ligA gene encoding the NAD-dependent DNA ligase from the Arctic bacterium Pseudoalteromonas haloplanktis was substituted for its homolog in the chromosome of Mycobacterium smegmatis. The resulting recombinant strain grew identically to the parent strain at permissive temperatures but failed ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv152

    authors: Pinto CT,Nano FE

    更新日期:2015-09-01 00:00:00

  • A species of magnetotactic deltaproteobacterium was detected at the highest abundance during an algal bloom.

    abstract::Magnetotactic bacteria (MTB) are a group of microorganisms that have the ability to synthesize intracellular magnetic crystals (magnetosomes). They prefer microaerobic or anaerobic aquatic sediments. Thus, there is growing interest in their ecological roles in various habitats. In this study we found co-occurrence of ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz253

    authors: Pan H,Dong Y,Teng Z,Li J,Zhang W,Xiao T,Wu LF

    更新日期:2019-11-01 00:00:00

  • SopB effector protein of Salmonella Typhimurium is translocated in mesenteric lymph nodes during murine salmonellosis.

    abstract::Salmonella Typhimurium harbors two Salmonella pathogenicity islands (SPIs), each encoding a type three secretion system for virulence proteins. Although there is increasing evidence of postinvasion roles for SPI-1, it has been generally accepted that SPI-1 genes are downregulated following the invasion process. Here, ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02217.x

    authors: Giacomodonato MN,Sarnacki SH,Llana MN,Cerquetti MC

    更新日期:2011-04-01 00:00:00

  • Isolation of a class IV chitin synthase gene from a zygomycete fungus, Rhizopus oligosporus.

    abstract::We found the presence of DNA sequence which shows sequence similarity to the class IV chitin synthase gene (CHS3) of Saccharomyces cerevisiae in the genome of 14 Rhizopus species which belong to zygomycetes. We cloned a gene (chs3), which might correspond to one of these homologous sequences, from Rhizopus oligosporus...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13291.x

    authors: Motoyama T,Horiuchi H,Ohta A,Yamaguchi I,Takagi M

    更新日期:1998-12-01 00:00:00

  • The effects of oligosaccharide trimming inhibitors on glycoprotein expression and infectivity of Junin virus.

    abstract::The effects of specific inhibitors of glycoprotein trimming reactions on Junin virus (JV) replication were investigated. Bromoconduritol, an inhibitor of glucosidase II, significantly reduced infective virus production (DE50: 1.1 mM) and viral protein expression. Neither 1-deoxynojirimycin, an inhibitor of both glucos...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06140.x

    authors: Silber AM,Candurra NA,Damonte EB

    更新日期:1993-05-01 00:00:00

  • Evidence that the Piromyces gene family encoding endo-1,4-mannanases arose through gene duplication.

    abstract::The sequences of two Piromyces cDNAs (manB and manC) encoding functional mannanases, defined as mannanase B (MANB) and mannanase C (MANC), revealed that both the cDNAs, and the encoded enzymes, exhibited extensive sequence identity with each other and with a previously described Piromyces mannanase. MANB and MANC, whi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08382.x

    authors: Millward-Sadler SJ,Hall J,Black GW,Hazlewood GP,Gilbert HJ

    更新日期:1996-08-01 00:00:00

  • Delineation of species within the Trichoderma viride/atroviride/koningii complex by UP-PCR cross-blot hybridization.

    abstract::Sequences from the ribosomal DNA ITS regions have shown that there is limited variation among the species in the Trichoderma viride/atroviride/koningii species complex also known as Hypocrea rufa complex, and that infraspecies variation sometimes is larger than interspecies variation. Strains belonging to T. viride, T...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.06.041

    authors: Lübeck M,Bulat S,Alekhina I,Lieckfeldt E

    更新日期:2004-08-15 00:00:00

  • A new insertion sequence from Sinorhizobium meliloti with homology to IS1357 from Methylobacterium sp. and IS1452 from Acetobacter pasteurianus.

    abstract::The insertion sequence ISRm8 was identified by sequence analysis of the cryptic plasmid pRmeGR4b of Sinorhizobium meliloti GR4. ISRm8 is 1451 bp in length and carries 22/24-bp terminal imperfect inverted repeats with seven mismatches and a direct target site duplication of 3 bp. ISRm8 carries a unique open reading fra...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb12804.x

    authors: Zekri S,Toro N

    更新日期:1998-01-01 00:00:00

  • From bioremediation to biowarfare: on the impact and mechanism of type IV secretion systems.

    abstract::Type IV secretion systems are employed by a wide variety of Gram-negative microorganisms for the translocation of macromolecules across the cell envelope. The translocated substrates (proteins, protein-DNA complexes and DNA) are as diverse as the organisms on the donor and recipient side of the translocation process. ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1016/j.femsle.2005.09.030

    authors: Baron C

    更新日期:2005-12-15 00:00:00

  • The intracellular proteolytic system of Yarrowia lipolytica and characterization of an aminopeptidase.

    abstract::Intracellular proteases of Yarrowia lipolytica have been scarcely studied. These enzymes may play an important role in nitrogen metabolism, posttranslational processing, nutritional stress, dimorphism, etc.; biochemical and genetic control of these enzymes can help in obtaining high-level expression of recombinant pro...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00578.x

    authors: Hernández-Montañez Z,Araujo-Osorio J,Noriega-Reyes Y,Chávez-Camarillo G,Villa-Tanaca L

    更新日期:2007-03-01 00:00:00

  • Molecular host mimicry and manipulation in bacterial symbionts.

    abstract::It is common among intracellular bacterial pathogens to use eukaryotic-like proteins that mimic and manipulate host cellular processes to promote colonization and intracellular survival. Eukaryotic-like proteins are bacterial proteins with domains that are rare in bacteria, and known to function in the context of a eu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fnz038

    authors: Frank AC

    更新日期:2019-02-01 00:00:00

  • Differences in Fe(III) reduction in the hyperthermophilic archaeon, Pyrobaculum islandicum, versus mesophilic Fe(III)-reducing bacteria.

    abstract::The discovery that all hyperthermophiles that have been evaluated have the capacity to reduce Fe(III) has raised the question of whether mechanisms for dissimilatory Fe(III) reduction have been conserved throughout microbial evolution. Many studies have suggested that c-type cytochromes are integral components in elec...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10529.x

    authors: Childers SE,Lovley DR

    更新日期:2001-02-20 00:00:00