Diversity of CTX-M beta-lactamases and their promoter regions from Enterobacteriaceae isolated in three Parisian hospitals.

Abstract:

:Nine clinical isolates of Enterobacteriaceae (six Escherichia coli and three Proteus mirabilis) isolated in three Parisian hospitals between 1989 and 2000 showed a particular extended-spectrum cephalosporin-resistance profile characterized by resistance to cefotaxime and aztreonam but not to ceftazidime. CTX-M-1, CTX-M-2, CTX-M-9, CTX-M-14 and two novel plasmid-mediated CTX-M beta-lactamases (CTX-M-20, and CTX-M-21) were identified by polymerase chain reaction and isoelectric focusing (pI>8) and were associated in eight cases with TEM-1 (pI=5.4) or TEM-2 (pI=5.6) beta-lactamases. We used internal ISEcp1 and IS26 forward primers and the CTX-M consensus reverse primer to characterize the CTX-M beta-lactamase promoter regions and showed their high degree of structure diversity. We found upstream of some bla(CTX-M) genes, a 266-bp sequence 100% identical to the sequence upstream of the Kluyvera ascorbata beta-lactamase gene, suggesting that this chromosomal enzyme is the progenitor of the CTX-M-2/5 cluster.

journal_name

FEMS Microbiol Lett

authors

Saladin M,Cao VT,Lambert T,Donay JL,Herrmann JL,Ould-Hocine Z,Verdet C,Delisle F,Philippon A,Arlet G

doi

10.1111/j.1574-6968.2002.tb11126.x

keywords:

subject

Has Abstract

pub_date

2002-04-09 00:00:00

pages

161-8

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378109702004846

journal_volume

209

pub_type

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