Diversity of CTX-M beta-lactamases and their promoter regions from Enterobacteriaceae isolated in three Parisian hospitals.

abstract：:To improve expression efficiency of the leuB gene of an extreme thermophile. Thermus thermophilus, in Escherichia coli, the gene was placed under a potent promoter, tac. However, the expression was hardly improved, despite increased transcription. The expression under tac promoter was significantly improved by introdu...

journal_title：FEMS microbiology letters

authors： Ishida M,Oshima T

更新日期：1996-01-01 00:00:00

abstract：:The glycoside hydrolase (GH) 16 family of Coprinopsis cinerea includes 15 members distributed in four subgroups (A1, A2, B and D) by phylogenetic analysis. The expression patterns match well with the requirement of wall-softening in the germination of basidiospores, hyphal growth and branching, primordium formation, s...

journal_title：FEMS microbiology letters

authors： Kang L,Zhu Y,Bai Y,Yuan S

更新日期：2019-04-01 00:00:00

abstract：:We present evidence that glycine betaine (betaine) which was synthesized from choline was excreted and reaccumulated in osmoregulating cells of Escherichia coli. Choline which was accumulated in bet mutants defective in betaine synthesis was shown to be excreted in response to betaine uptake. Our data suggest that E. ...

journal_title：FEMS microbiology letters

authors： Lamark T,Styrvold OB,Strøm AR

更新日期：1992-09-15 00:00:00

abstract：:We cloned and characterized the Neisseria meningitidis rfaC gene which encodes an enzyme, alpha-1,5 heptosyltransferase I, involved in the synthesis of the deep-core of the lipooligosaccharide. The N. meningitidis rfaC mutant, obtained by an allelic exchange, produced lipooligosaccharide which migrated faster in sodiu...

journal_title：FEMS microbiology letters

authors： Stojiljkovic I,Hwa V,Larson J,Lin L,So M,Nassif X

更新日期：1997-06-01 00:00:00

abstract：:Twelve strains of Candida albicans were grown in defined medium which had been deferrated by ion-exchange chromatography and then supplemented with FeCl3 to give iron concentrations ranging from 0.026 microM (growth-limiting) to 0.8 microM (excess). All of the strains secreted hydroxamate-type siderophores; phenolate ...

journal_title：FEMS microbiology letters

authors： Sweet SP,Douglas LJ

更新日期：1991-05-01 00:00:00

abstract：:CRISPR-Cas adaptive immune systems protect bacteria from phage predation, and other foreign genetic elements such as plasmids. Significant advances have been made regarding how CRISPR-Cas systems elicit immunity; however, comparatively little is known about their regulation. To study CRISPR-Cas regulation, we describe...

journal_title：FEMS microbiology letters

authors： Hampton HG,Patterson AG,Chang JT,Taylor C,Fineran PC

更新日期：2019-06-01 00:00:00

abstract：:A [Fe]-hydrogenase gene (hydA) was cloned from Clostridium paraputrificum M-21 in Escherichia coli using a conserved DNA sequence of clostridial hydrogenase genes amplified by PCR as the probe. The hydA gene consisted of an open reading frame of 1749 bp encoding 582 amino acids with an estimated molecular mass of 64,5...

journal_title：FEMS microbiology letters

authors： Morimoto K,Kimura T,Sakka K,Ohmiya K

更新日期：2005-05-15 00:00:00

abstract：:Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot disease in cruciferous plants. The synthesis of known virulence factors in this organism, such as extracellular enzymes and biofilm, is strictly regulated in response to environmental stimuli. Two-component signal transduction systems sense e...

journal_title：FEMS microbiology letters

authors： Tao J,He C

更新日期：2010-03-01 00:00:00

abstract：:A derivative of Staphylococcus simulans biovar staphylolyticus cured of all five plasmids present in the wild-type organism was developed, and the characteristics of extracellular protein production by this plasmidless strain were compared to those of the wild type. Although staphylolytic endopeptidase (lysostaphin) a...

journal_title：FEMS microbiology letters

authors： Nitterauer JD,Heath HE,Heath LS,LeBlanc PA,Sloan GL

更新日期：1991-11-01 00:00:00

abstract：:Bacteria have the exquisite ability to maintain a precise diameter, cell length, and shape. The dimensions of bacteria size and shape are a classical metric in the distinction of bacterial species. Much of what we know about the particular morphology of any given species is the result of investigations of planktonic c...

journal_title：FEMS microbiology letters

authors： Justice SS,Harrison A,Becknell B,Mason KM

更新日期：2014-11-01 00:00:00

abstract：:Nocardia asteroides complex organisms derived from human specimens between 1979 and 1992 were identified to the species level. Of 117 N. asteroides complex organisms, 34 (29%) were N. farcinica, 28 (24%) were N. nova, and 55 (47%) were N. asteroides sensu stricto. An analysis of the specimen sites from which the organ...

journal_title：FEMS microbiology letters

authors： Desmond EP,Flores M

更新日期：1993-07-01 00:00:00

abstract：:We analyzed gel electrophoretic banding patterns of penicillin-binding proteins (PBPs) of 16 type strains of coagulase-negative staphylococci (CNS). S. epidermidis, S. haemolyticus, S. saprophyticus, S. hominis, S. xylosus, S. simulans, S. warneri, S. capitis, S. saccharolyticus, S. auricularis, S. caseolyticus, S. ga...

journal_title：FEMS microbiology letters

authors： Kanda K,Hiramatsu K,Yokota T

更新日期：1990-10-01 00:00:00

abstract：:The enzymatic activity of salivary amylase bound to the surface of several species of oral streptococci was determined by the production of acid from starch and by the degradation of maltotetraose to glucose in a coupled, spectrophotometric assay. Most strains able to bind amylase exhibited functional enzyme on their ...

journal_title：FEMS microbiology letters

authors： Douglas CW,Heath J,Gwynn JP

更新日期：1992-04-15 00:00:00

abstract：:The significance of the chronicled role of the yeast transcription factor Adr1p in regulating ETR1 was examined in wild type and isogenic adr1Delta mutant cells. An ETR1-lacZ reporter construct was used to verify Adr1p-dependent gene expression. On solid glycerol medium containing X-gal, wild-type cells expressing the...

journal_title：FEMS microbiology letters

authors： Gurvitz A

更新日期：2009-08-01 00:00:00

abstract：:We have established an improved large deletion method in Escherichia coli genome using a combination of two different recombination systems, lambda Red and Cre/loxP. The loxP site could be rapidly and efficiently integrated in the genome by lambda Red and large deletions of both 117- and 165-kbp regions could be gener...

journal_title：FEMS microbiology letters

authors： Fukiya S,Mizoguchi H,Mori H

更新日期：2004-05-15 00:00:00

abstract：:Brucella melitensis possesses an operon with two components: the response regulator OtpR and a putative cAMP-dependent protein kinase regulatory subunit encoded by the BMEI0067 gene. In the previous study, the function of OtpR has been studied, while little is known about the function of the BMEI0067 gene. Using a bio...

journal_title：FEMS microbiology letters

authors： Liu WJ,Dong H,Peng XW,Wu QM

更新日期：2014-07-01 00:00:00

abstract：:One of the commercial strains of Flammulina velutipes was analyzed for the presence of double-stranded RNA (dsRNA) elements to examine the underlying mechanism of strain degeneration. As a result, two dsRNA elements sized 1.9 and 1.8 kb were detected in mycelium derived from spontaneously brown-colored fruit body. The...

journal_title：FEMS microbiology letters

authors： Magae Y,Hayashi N

更新日期：1999-11-15 00:00:00

abstract：:The porin-associated protein of Rhodospirillum rubrum FR1 was found to contain a peptidoglycan binding motif. A partial fragment of 179 amino acids, obtained by cleavage of PAP with trypsin, Asp-N protease, and CNBr, was sequenced. Substantial sequence homology was found of the C-terminal part (residues 126-179) of po...

journal_title：FEMS microbiology letters

authors： Neumann U,Schiltz E,Stahl B,Hillenkamp F,Weckesser J

更新日期：1996-04-15 00:00:00

abstract：:Yeasts of various genera were screened for denitrifying activity, and several yeast strains such as Trichosporon cutaneum, Fellomyces fuzhouensis, and Candida sp. were found to exhibit distinct activities to convert nitrite to nitrous oxide. Dissimilatory nitrite reductase (Nir) or nitric oxide reductase (Nor) activit...

journal_title：FEMS microbiology letters

authors： Tsuruta S,Takaya N,Zhang L,Shoun H,Kimura K,Hamamoto M,Nakase T

更新日期：1998-11-01 00:00:00

abstract：:The lipid modification and processing of truncated mutants of the colicin A lysis protein were observed after overproduction by Escherichia coli bacteria, but at a rate far slower than that of the wild-type. The unmodified precursor form of the mutants was stable over hour(s). The truncated mutants provoked lethality,...

journal_title：FEMS microbiology letters

authors： Cavard D

更新日期：1994-04-01 00:00:00

abstract：:Sera, from 17 patients with diarrhoea or haemolytic uraemic syndrome, and six healthy adults, were tested for neutralisation of Vero cytotoxins (VT). For all 17 patients there was evidence of infection with Escherichia coli O157. Sera from two controls but from none of the patients neutralised VT1, although two patien...

journal_title：FEMS microbiology letters

authors： Scotland SM,Said B,Thomas A,Rowe B

更新日期：1994-01-15 00:00:00

abstract：:Xanthomonas oryzae pv. oryzae is an important plant pathogen which causes bacterial blight of rice. To facilitate genome studies of this bacterium, we have constructed a bacterial artificial chromosome (BAC) library of strain MAFF 311018. It consisted of 750 clones representing 16 genome equivalents, and had an insert...

journal_title：FEMS microbiology letters

authors： Ochiai H,Inoue Y,Hasebe A,Kaku H

更新日期：2001-06-12 00:00:00

abstract：:The genus Pycnoporus forms a group of four species known especially for producing high redox potential laccases suitable for white biotechnology. A sample of 36 Pycnoporus strains originating from different geographical areas was studied to seek informative molecular markers for the typing of new strains in laboratory...

journal_title：FEMS microbiology letters

authors： Lesage-Meessen L,Haon M,Uzan E,Levasseur A,Piumi F,Navarro D,Taussac S,Favel A,Lomascolo A

更新日期：2011-12-01 00:00:00

abstract：:Trehalase was studied in Schizosaccharomyces pombe cells growing vegetatively on minimal medium and in sporulating cultures. Acid trehalase activity, measured at pH 4.2, was absent in vegetative cells and occurred only in asci, indicating that this activity represented the sporulation-specific trehalase reported previ...

journal_title：FEMS microbiology letters

authors： De Virgilio C,Müller J,Boller T,Wiemken A

更新日期：1991-11-01 00:00:00

abstract：:An identification method by PCR, specific to the Carnobacterium genus, was optimised by testing it on 28 bacterial strains. Primers from the literature were tested to differentiate Carnobacterium strains present among various bacterial species. The DNA of Carnobacterium species (C. alterfunditum, C. divergens, C. fund...

journal_title：FEMS microbiology letters

authors： Cailliez-Grimal C,Miguindou-Mabiala R,Leseine M,Revol-Junelles AM,Millière JB

更新日期：2005-09-01 00:00:00

abstract：:Vf33 is a filamentous bacteriophage isolated from Vibrio parahaemolyticus. We performed Southern blot hybridization analysis to examine the distribution of Vf33-related genetic elements in the pandemic strains (O3:K6 strains isolated between 1995 and 1997, O4:K68 and O1:K untypeable strains isolated between 1997 and 1...

journal_title：FEMS microbiology letters

authors： Chang B,Yoshida S,Miyamoto H,Ogawa M,Horikawa K,Ogata K,Nishibuchi M,Taniguchi H

更新日期：2000-11-15 00:00:00

abstract：:Three (0140J, C197C and EF20) out of four strains of Streptococcus uberis exhibited high levels of bound plasmin activity following growth in the presence of bovine plasminogen. The remaining strain (C197) bound considerably less plasmin following growth in the same medium. In contrast to the others, this strain was u...

journal_title：FEMS microbiology letters

authors： Leigh JA,Lincoln RA

更新日期：1997-09-01 00:00:00

abstract：:The SbmA protein is involved in the transport of MccB17-, MccJ25-, bleomycin- and proline-rich peptides into the Escherichia coli cytoplasm. sbmA gene homologues were found in a variety of bacteria. However, the physiological role of this protein still remains unknown. Previously, we found that a combination of sbmA a...

journal_title：FEMS microbiology letters

authors： Corbalán NS,Adler C,de Cristóbal RE,Pomares MF,Delgado MA,Vincent PA

更新日期：2010-10-01 00:00:00

abstract：:The genus Phytomonas is responsible for many diseases in different crop plant species. The finding that chitin is an exposed cell surface polysaccharide in Phytomonas françai and the observation that chitinases can inhibit fungal growth raises expectations about the potential effect of plant chitinases on the P. franç...

journal_title：FEMS microbiology letters

authors： Gomes Rocha GC,Nicolich R,Romeiro A,Margis-Pinheiro M,Attias M,Alves-Ferreira M

更新日期：2003-09-12 00:00:00

abstract：:It is now well established that physicochemical properties of exopolysaccharides (EPS) can vary between strains of a given species and according to growth conditions. The EPS production of four strains of Lactobacillus bulgaricus was monitored during growth in milk and in a chemically defined media. All strains, inclu...

journal_title：FEMS microbiology letters

authors： Petry S,Furlan S,Waghorne E,Saulnier L,Cerning dagger J,Maguin E

更新日期：2003-04-25 00:00:00