Corynebacterium diphtheriae surface proteins as adhesins to human erythrocytes.

abstract：:Bifidobacteria are normal inhabitants of the human gut, and members of which are generally considered to be probiotic. Before exerting their beneficial properties, they must survive and persist in the physiological concentrations (0.05-2%) of bile in the gut. In this work, the functional role of tlyC1 encoding a hemol...

journal_title：FEMS microbiology letters

authors： Liu Y,An H,Zhang J,Zhou H,Ren F,Hao Y

更新日期：2014-11-01 00:00:00

abstract：:We have constructed a DNA library from a virulent Salmonella typhimurium strain, in an avirulent strain. The process of selecting the components of interest from the library involved iterative growth in liquid culture. This resulted, after four cycles, in the culture becoming homogeneous for a single plasmid, which wa...

journal_title：FEMS microbiology letters

authors： Lodge JM,Robey M,Stephen J,Brown NL

更新日期：1999-01-15 00:00:00

abstract：:The effect of low concentrations of cyanide on dissimilatory perchlorate and chlorate reduction and aerobic respiration was examined using pure cultures of Azospira sp. KJ. Cyanide at a concentration of 38 microM inhibited cell growth on perchlorate, chlorate and molecular oxygen, but it did not inhibit the activity o...

journal_title：FEMS microbiology letters

authors： Song Y,Logan BE

更新日期：2004-10-15 00:00:00

abstract：:Recent molecular approaches for the study of microbial communities such as PCR-cloning have enabled the detection and identification of as-yet-unculturable taxa. Cloning and sequencing of multiple samples is extremely laborious and expensive to perform thoroughly due to the large diversity involved. For this purpose, ...

journal_title：FEMS microbiology letters

authors： Gafan GP,Spratt DA

更新日期：2005-12-15 00:00:00

abstract：:Enterobacter cloacae CHE, a clinical strain with overproduced cephalosporinase was found to be highly resistant to the new cephalosporins, cefepime and cefpirome (MICs> or =128 microg ml(-1)). The strain was isolated from a child previously treated with cefepime. The catalytic efficiency of the purified enzyme with th...

journal_title：FEMS microbiology letters

authors： Barnaud G,Labia R,Raskine L,Sanson-Le Pors MJ,Philippon A,Arlet G

更新日期：2001-02-20 00:00:00

abstract：:There are no published methods on plasmid isolation from Peptostreptococcus spp., therefore two methods of plasmid isolation from this genera were analysed: the boiling and alkaline-SDS methods. Plasmid DNA was not recovered by the boiling method, however, with the alkaline-SDS method, cryptic plasmid DNA was detected...

journal_title：FEMS microbiology letters

authors： Ng LK,Dillon JA

更新日期：1989-10-01 00:00:00

abstract：:Carbohydrate-binding components were shown to be present at the surface of Listeria monocytogenes by means of a panel of neoglycoproteins using direct agglutination. These lectin-like components bind on neoglycoproteins bearing D-glucosamine, L-fucosylamine, or para-amino-phenyl-alpha-D-mannopyrannoside residues. The ...

journal_title：FEMS microbiology letters

authors： Cottin J,Loiseau O,Robert R,Mahaza C,Carbonnelle B,Senet JM

更新日期：1990-03-15 00:00:00

abstract：:A lipopolysaccharide mutant of Leptospira interrogans (LaiMut) was obtained by growth in the presence of an agglutinating monoclonal antibody (mAb) against lipopolysaccharide. Agglutination reactions with anti-lipopolysaccharide mAbs and polyclonal antibodies showed that LaiMut had lost some serogroup Icterohaemorrhag...

journal_title：FEMS microbiology letters

authors： Zapata S,Trueba G,Bulach DM,Boucher D,Adler B,Hartskeerl R

更新日期：2010-08-01 00:00:00

abstract：:The filamentous ascomycete Botrytis cinerea is one of the most studied models for understanding the necrotrophic behaviour of phytopathogenic fungi. The genomes of two strains of B. cinerea have been sequenced (B05.10 and T4), which may contribute to elucidating the virulence polymorphism in this fungus. In this study...

journal_title：FEMS microbiology letters

authors： Choquer M,Robin G,Le Pêcheur P,Giraud C,Levis C,Viaud M

更新日期：2008-12-01 00:00:00

abstract：:The hybrid Pseudomonas cepacia strain JHR22 was tested for its ability to degrade Aroclor 1221 in soil. The influence of supplements--mineral salts and trace elements--on the degradation was investigated. Disappearance of Aroclor 1221 congeners, occurrence of metabolites, and release of chloride were measured under di...

journal_title：FEMS microbiology letters

authors： Havel J,Reineke W

更新日期：1993-04-01 00:00:00

abstract：:Clostridium difficile and Clostridium perfringens type A are infectious agents of enteric diseases. The main virulence factors of these microorganisms include toxins A and B of C. difficile (ToxA and ToxB) and enterotoxin of C. perfringens (Cpe). In this study genetic constructions have been created for the expression...

journal_title：FEMS microbiology letters

authors： Belyi IF,Varfolomeeva NA

更新日期：2003-08-29 00:00:00

abstract：:Insertion sequence IS100 was localized on a 9.5-kb plasmid of Yersinia pestis and was shown to be specific for Y. pestis and serotype I strains of Y. pseudotuberculosis. The nucleotide sequence of IS100 isolated from this plasmid was determined. The element, which was flanked by 5-bp direct repeats, contained 1953 bp ...

journal_title：FEMS microbiology letters

authors： Podladchikova ON,Dikhanov GG,Rakin AV,Heesemann J

更新日期：1994-09-01 00:00:00

abstract：:Two type B15 P1.16 strains of Neisseria meningitidis were examined by immunogold electron microscopy for accessibility of two outer membrane protein (OMPs) to monoclonal antibodies. Both strains exhibited cell-to-cell variation of one epitope of the Class 3 OMP (P3.15) and one of the Class 1 OMPs (P1.16) when grown in...

journal_title：FEMS microbiology letters

authors： Wall RA,Davies HA,Borriello SP

更新日期：1989-11-01 00:00:00

abstract：:Saccharomyces cerevisiae was engineered for assembly of minicellulosomes by heterologous expression of a recombinant scaffolding protein from Clostridium cellulovorans and a chimeric endoglucanase E from Clostridium thermocellum. The chimeric endoglucanase E fused with the dockerin domain of endoglucanase B from C. ce...

journal_title：FEMS microbiology letters

authors： Hyeon JE,Yu KO,Suh DJ,Suh YW,Lee SE,Lee J,Han SO

更新日期：2010-09-01 00:00:00

abstract：:A transformation system for Moorella thermoacetica ATCC39073 was developed using thermostable kanamycin resistant gene (kanR) derived from the plasmid pJH1 that Streptococcus faecalis harbored. When kanR with its native promoter was introduced into uracil auxotrophic mutant of M. thermoacetica ATCC39073 together with ...

journal_title：FEMS microbiology letters

authors： Iwasaki Y,Kita A,Sakai S,Takaoka K,Yano S,Tajima T,Kato J,Nishio N,Murakami K,Nakashimada Y

更新日期：2013-06-01 00:00:00

abstract：:beta-Glucosidase in Aspergillus nidulans was found to be both intracellular and extracellular. The intracellular beta-glucosidase was synthesized after the exhaustion of carbon source in the medium. The extracellular enzyme appeared with autolysis of the mycelium. Biosynthesis of beta-glucosidase was not induced by va...

journal_title：FEMS microbiology letters

authors： Lee J,Kwon KS,Hah YC

更新日期：1996-01-01 00:00:00

abstract：:The aim of this study was the production of the homopolyester poly(4-hydroxybutyric acid) (poly(4HB)) with recombinant strains of Escherichia coli. Wild-type strains and other widely used non-recombinant strains of E. coli are not able to produce polyhydroxyalkanoic acids (PHA) as storage compounds and cannot utilize ...

journal_title：FEMS microbiology letters

authors： Hein S,Söhling B,Gottschalk G,Steinbüchel A

更新日期：1997-08-15 00:00:00

abstract：:To clarify the cause of the supersusceptibility of Branhamella catarrhalis to macrolide antibiotics, which are well-known to be inactive to most Gram-negative bacteria, we determined its cell surface hydrophobicity by the partition experiment between water and hydrocarbons. Its cell surface was found to be markedly mo...

journal_title：FEMS microbiology letters

authors： Gotoh N,Tanaka S,Nishino T

更新日期：1989-05-01 00:00:00

abstract：:Based on amino acid sequence similarities between the methylated elongation factor EF-Tu from Escherichia coli and the EF-Tu from Euglena gracilis chloroplast, we predicted that the latter could also be methylated in the presence of an appropriate methyltransferase. We found that, as reported for the eubacterial homol...

journal_title：FEMS microbiology letters

authors： Toledo H,Jerez CA

更新日期：1990-09-15 00:00:00

abstract：:The nucleotide sequence of the gene specifying the ethidium efflux system of Escherichia coli has been determined. The translated open reading frame has identified a membrane-bound polypeptide of 110 amino acids (11,960 Da) which shares 42% identity with a staphylococcal protein specifying resistance to ethidium. ...

journal_title：FEMS microbiology letters

authors： Purewal AS

更新日期：1991-08-01 00:00:00

abstract：:Corynebacterium diphtheriae, generally considered an extracellular coloniser, was evaluated for its ability to enter and survive within HEp-2 monolayers by gentamicin protection assay. Intracellular viability of HC01 strain, isolated from endocarditis, was more expressive (2.59%) than observed in 241 (0.21%) and CDC-E...

journal_title：FEMS microbiology letters

authors： Hirata R,Napoleão F,Monteiro-Leal LH,Andrade AF,Nagao PE,Formiga LC,Fonseca LS,Mattos-Guaraldi AL

更新日期：2002-09-24 00:00:00

abstract：:In this study, a putative N-acetylmuramyl-l-alanine amidase gene (bb0666) was identified in the genome of the Lyme disease spirochete Borrelia burgdorferi. This protein shares c. 30% identity with its counterparts from other bacteria. Reverse transcriptase-PCR analysis showed that bb0666 along with two other genes (bb...

journal_title：FEMS microbiology letters

authors： Yang Y,Li C

更新日期：2009-01-01 00:00:00

abstract：:The outer membrane protein (OMP) profiles of four different strains of Bacteroides fragilis, as determined by Coomassie blue stained polyacrylamide gels, were compared after growth in broth culture and in the mouse peritoneal cavity. There was no induction of the expression of large quantities of novel OMP after growt...

journal_title：FEMS microbiology letters

authors： Patrick S,Lutton DA

更新日期：1990-09-01 00:00:00

abstract：:Weissella cibaria isolated from human saliva produces a soluble glucan that predominantly has alpha-1,6-glucosidic type linkages. Using degenerated primers that were selected based on the amino acid sequences of conserved regions from known glucansucrases, a single 2.7-kb fragment was isolated. In subsequent steps, a ...

journal_title：FEMS microbiology letters

authors： Kang HK,Oh JS,Kim D

更新日期：2009-03-01 00:00:00

abstract：:pSA1.1 is a 9.1-kb multicopy plasmid originally isolated from Streptomyces cyaneus (formerly S. azureus) ATCC 14921. This plasmid accumulates single-stranded DNA in S. lividans and is therefore considered to replicate by a rolling-circle replication. In the present work, the rep gene encoding the replication initiator...

journal_title：FEMS microbiology letters

authors： Yokoyama E,Matsuzaki Y,Doi K,Ogata S

更新日期：1998-12-01 00:00:00

abstract：:Chemical and molecular taxonomic studies were performed on a representative strain of some lactic acid bacteria of unknown taxonomic position isolated from salmonid fish. The results demonstrate that the fish bacterium represents a new species of the genus Lactococcus for which the name Lactococcus piscium sp. nov. is...

journal_title：FEMS microbiology letters

authors： Williams AM,Fryer JL,Collins MD

更新日期：1990-03-01 00:00:00

abstract：:A total of 401 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates from two experimentally infected calves were analyzed using molecular biological methods. Genetic differences detected by pulsed-field gel electrophoresis were observed between the inoculated and recovered strains as early as 1 day post inoculat...

journal_title：FEMS microbiology letters

authors： Akiba M,Sameshima T,Nakazawa M

更新日期：2000-03-01 00:00:00

abstract：:Xylella fastidiosa is the causal agent of economically important plant diseases, including citrus variegated chlorosis and Pierce's disease. Hitherto, there has been no information on the molecular mechanisms controlling X. fastidiosa-plant interactions. To determine whether predicted open reading frames (ORFs) encodi...

journal_title：FEMS microbiology letters

authors： Scarpari LM,Lambais MR,Silva DS,Carraro DM,Carrer H

更新日期：2003-05-16 00:00:00

abstract：:We present evidence that glycine betaine (betaine) which was synthesized from choline was excreted and reaccumulated in osmoregulating cells of Escherichia coli. Choline which was accumulated in bet mutants defective in betaine synthesis was shown to be excreted in response to betaine uptake. Our data suggest that E. ...

journal_title：FEMS microbiology letters

authors： Lamark T,Styrvold OB,Strøm AR

更新日期：1992-09-15 00:00:00

abstract：:Characterization of genomic variation among different microbial species, or different strains of the same species, is a field of significant interest with a wide range of potential applications. We have investigated the genomic variation in mycorrhizal fungal genomes through genomic suppressive subtractive hybridizati...

journal_title：FEMS microbiology letters

authors： Murat C,Zampieri E,Vallino M,Daghino S,Perotto S,Bonfante P

更新日期：2011-05-01 00:00:00