Nucleotide sequence and structural organization of Yersinia pestis insertion sequence IS100.

abstract：:The distribution of virulence markers related to cytolethal distending toxin-V (CDT-V), subtilase cytotoxin (SubAB), the enterohemorrhagic Escherichia coli factor for adherence (Efa1), the adhesin similar to IrgA (Iha), the long polar fimbriae (LpfO113), the autoagglutinating adhesin (Saa), and the protein required fo...

journal_title：FEMS microbiology letters

authors： Cergole-Novella MC,Nishimura LS,Dos Santos LF,Irino K,Vaz TM,Bergamini AM,Guth BE

更新日期：2007-09-01 00:00:00

abstract：:Cell walls of Microcystis sp. PCC 7806 were purified from cell homogenates by sucrose density centrifugation and Triton X-100 extraction. The outer membrane contained carotenoids, two major peptidoglycan-associated proteins (Mr 49,000 and 52,000), and lipopolysaccharide (LPS) as indicated by the presence of 3-hydroxy ...

journal_title：FEMS microbiology letters

authors： Jürgens UJ,Martin C,Weckesser J

更新日期：1989-11-01 00:00:00

abstract：:This commentary describes an assessment exercise known as the TRIPSE (Tri-Partite Problem Solving Exercise) that mimics science in operation. Students frame hypotheses based on limited data, design experiments to test them, which they later revise with new information. It is emphasised that there are no single correct...

journal_title：FEMS microbiology letters

authors： Nastos S,Rangachari PK

更新日期：2016-08-01 00:00:00

abstract：:The CotSA protein, encoded by cotSA (ytxN) of Bacillus subtilis, was detected from the cells at 5 h after the onset of sporulation (T5) and in the spore coat of wild-type cells, but not in cotE, cotS, gerE, or cotSA mutant spores. CotSA was also detected in the sporangium at T5 to T7 but not in the sporangium at T18 o...

journal_title：FEMS microbiology letters

authors： Takamatsu H,Kodama T,Watabe K

更新日期：1999-05-01 00:00:00

abstract：:To tackle antimicrobial resistance (AMR) is of outmost importance for the general population to understand the severity and the relevance of different routes of transmission. Respondents of different age groups, educational and occupational backgrounds, area of living, diet and household composition participated in an...

journal_title：FEMS microbiology letters

authors： Alexa Oniciuc EA,Likotrafiti E,Garre A,Ruiz L,Prieto M,Alvarez-Ordóñez A

更新日期：2019-09-01 00:00:00

abstract：:We report the effects of dissolved oxygen (DO) concentration and iron addition on gene expression of Magnetospirillum gryphiswaldense MSR-1 cells during fermentations, focusing on 0.25-24 h after iron addition. The DO was strictly controlled at 0.5% or 5% O2, and compared with aerobic condition. Uptake of iron (and fo...

journal_title：FEMS microbiology letters

authors： Zhuang S,Anyaogu DC,Kasama T,Workman M,Mortensen UH,Hobley TJ

更新日期：2017-06-15 00:00:00

abstract：:The role of the flagellum and chemotactic motility of Vibrio anguillarum for phagocytosis by and intracellular survival in fish macrophages was determined using a wild-type strain, a mutant without the flagellum, a mutant with a truncated flagellum and a non-chemotactic mutant. For all strains, the numbers of intracel...

journal_title：FEMS microbiology letters

authors： Larsen MH,Boesen HT

更新日期：2001-09-25 00:00:00

abstract：:A cDNA from Penicillium minioluteum HI-4 encoding a dextranase (1,6-alpha-glucan hydrolase, EC 3.2.1.11) was isolated and characterized. cDNA clones corresponding to genes expressed in dextran-induced cultures were identified by differential hybridization. Southern hybridization and restriction mapping analysis of sel...

journal_title：FEMS microbiology letters

authors： Garcia B,Margolles E,Roca H,Mateu D,Raices M,Gonzales ME,Herrera L,Delgado J

更新日期：1996-10-01 00:00:00

abstract：:The steady-state levels of the aminopeptidase Y (APY1) transcript and the level of assayable aminopeptidase Y activity were measured under a variety of nutritional conditions. Specific APY1 mRNA is less abundant in cells grown in minimal medium than in cells grown in rich medium, while active enzyme levels follow just...

journal_title：FEMS microbiology letters

authors： Herrera-Camacho I,Suárez-Rendueles P

更新日期：1996-06-01 00:00:00

abstract：:Lyme disease is a multisystemic disorder caused by Borrelia burgdorferi, an invasive spirochete. B. burgdorferi has a predilection for collagenous tissue and one major clinical manifestation of the disease is arthritis. We have identified a collagenolytic activity in B. burgdorferi detergent lysates using iodinated ge...

journal_title：FEMS microbiology letters

authors： Grab DJ,Kennedy R,Philipp MT

更新日期：1996-10-15 00:00:00

abstract：:A [Fe]-hydrogenase gene (hydA) was cloned from Clostridium paraputrificum M-21 in Escherichia coli using a conserved DNA sequence of clostridial hydrogenase genes amplified by PCR as the probe. The hydA gene consisted of an open reading frame of 1749 bp encoding 582 amino acids with an estimated molecular mass of 64,5...

journal_title：FEMS microbiology letters

authors： Morimoto K,Kimura T,Sakka K,Ohmiya K

更新日期：2005-05-15 00:00:00

abstract：:Studies on cultured cells and in infection models have shown that cell density-dependent quorum-sensing (QS) controls many of the known virulence factors of Pseudomonas aeruginosa. However, it is less clear what role QS plays in chronic human lung infections associated with cystic fibrosis (CF). The involvement of QS ...

journal_title：FEMS microbiology letters

authors： Winstanley C,Fothergill JL

更新日期：2009-01-01 00:00:00

abstract：:Streptomyces netropsis SD-07, the producer of novel polyene macrolide antifungal antibiotics, was isolated from soil. For the investigation of the functions of its biosynthesis genes and regulation mechanisms, a genetic operating system is necessary. In this study, we successfully transferred the plasmid DNA of pSET15...

journal_title：FEMS microbiology letters

authors： Wang XK,Jin JL

更新日期：2014-08-01 00:00:00

abstract：:A gene expression system for both Bacillus subtilis and Escherichia coli was developed. The expression vector, pHASH102, produces any combination of promoter and open reading frame to be expressed based on the T-extended cloning method. Because the pHASH series vectors are designed to shuttle between the genome and a ...

journal_title：FEMS microbiology letters

authors： Ohashi Y,Ohshima H,Tsuge K,Itaya M

更新日期：2003-04-11 00:00:00

abstract：:Recent studies have generated interest in the use of the homologous recombination system of bacteriophage lambda for genetic engineering. The system, called Red, consists primarily of three proteins: lambda exonuclease, which processively digests the 5'-ended strand of a dsDNA end; beta protein, which binds to ssDNA a...

journal_title：FEMS microbiology letters

authors： Poteete AR

更新日期：2001-07-10 00:00:00

abstract：:We have studied the single rRNA gene cluster from the Archaeon, Pyrococcus furiosus. This isolate grows optimally at 100 degrees C and is thus a hyperthermophile. In P. furiosus, transcription of 16S rRNA is subject to regulation over a 7.5-fold range in response to a 20-fold increase in growth rate. The single cluste...

journal_title：FEMS microbiology letters

authors： DiRuggiero J,Achenbach LA,Brown SH,Kelly RM,Robb FT

更新日期：1993-08-01 00:00:00

abstract：:Pseudomonas aeruginosa strains exhibiting a heterogeneous mode of growth against carbapenems have been described recently. This study investigated the underlying molecular mechanisms in four genetically unrelated P. aeruginosa clinical isolates that were previously characterized by population analyses as heterogeneous...

journal_title：FEMS microbiology letters

authors： Ikonomidis A,Tsakris A,Kantzanou M,Spanakis N,Maniatis AN,Pournaras S

更新日期：2008-02-01 00:00:00

abstract：:Riemerella anatipestifer is the causative agent of polyserositis of ducks and geese. We have previously reported that a 3.9-kb plasmid, pCFC1, carries protein genes (vapD1 and vapD2) that are similar to virulence-associated genes of other bacteria. In the present study, we report the complete sequence of a second plas...

journal_title：FEMS microbiology letters

authors： Weng S,Lin W,Chang Y,Chang C

更新日期：1999-10-01 00:00:00

abstract：:Nocardioides sp. strain MTD22 degraded atrazine, ametryn and atraton, as did Arthrobacter aurescens strain TC1 and Nocardioides sp. strain C190. These strains contain trzN, a gene coding for TrzN, triazine hydrolase showing a broad substrate range. However, Nocardioides sp. strain AN3 degraded only atrazine despite co...

journal_title：FEMS microbiology letters

authors： Yamazaki K,Fujii K,Iwasaki A,Takagi K,Satsuma K,Harada N,Uchimura T

更新日期：2008-09-01 00:00:00

abstract：:The relationship between sugar availability and RTX (repeats in toxin) cytotoxin (leukotoxin) production in the periodontopathic bacterium, Actinobacillus actinomycetemcomitans, was investigated using a chemostat. A actinomycetemcomitans 301-b produced significant amounts of leukotoxin in anaerobic fructose-limited ch...

journal_title：FEMS microbiology letters

authors： Mizoguchi K,Ohta H,Miyagi A,Kurihara H,Takashiba S,Kato K,Murayama Y,Fukui K

更新日期：1997-01-01 00:00:00

abstract：:Pseudomonas spp., in particular Pseudomonas fragi, are dominant in aerobically stored chilled meats. This work isolated P. fragi isolates from spoiled chicken and investigated the effect of modified atmosphere packaging (MAP, CO2/N2 30%/70%) on physicochemical properties of P. fragi isolates compared to the correspond...

journal_title：FEMS microbiology letters

authors： Wang GY,Li M,Ma F,Wang HH,Xu XL,Zhou GH

更新日期：2017-06-15 00:00:00

abstract：:In this study, 29 strains of Kluyveromyces marxianus with peculiar genetic and phenotypic traits previously isolated from a fermented goat milk of Yaghnob valley were investigated for chromosome length polymorphism (CLP) by PFGE, adhesion properties and carbon usage by Biolog analysis. Obtained data showed that strain...

journal_title：FEMS microbiology letters

authors： Perpetuini G,Tittarelli F,Mattarelli P,Modesto M,Cilli E,Suzzi G,Tofalo R

更新日期：2018-03-01 00:00:00

abstract：:The applicability of different PCR-based techniques, random amplified polymorphic DNA, cleaved amplified polymorphic sequence and SSRs (simple sequence repeats) to genetically identify, typify and discriminate among Saccharomyces cerevisiae strains, was investigated. The analysis and comparison of the genetic profiles...

journal_title：FEMS microbiology letters

authors： Pérez MA,Gallego FJ,Hidalgo P

更新日期：2001-12-18 00:00:00

abstract：:It is often taken for granted that all animals host and depend upon a microbiome, yet this has only been shown for a small proportion of species. We propose that animals span a continuum of reliance on microbial symbionts. At one end are the famously symbiont-dependent species such as aphids, humans, corals and cows, ...

journal_title：FEMS microbiology letters

authors： Hammer TJ,Sanders JG,Fierer N

更新日期：2019-05-01 00:00:00

abstract：:The time course production of xylanolytic enzymes by the rumen anaerobic fungus Neocallimastix frontalis was studied during growth on different carbon sources and revealed using isoelectric focusing and immunoblotting. A constant low level of endoxylanase expression was observed in glucose medium. A high level of xyla...

journal_title：FEMS microbiology letters

authors： Gomez de Segura B,Durand R,Fèvre M

更新日期：1998-07-01 00:00:00

abstract：:The devR-devS two-component system of Mycobacterium tuberculosis was identified earlier and partially characterized in our laboratory. A devR::kan mutant of M. tuberculosis was constructed by allelic exchange. The devR mutant strain showed reduced cell-to-cell adherence in comparison to the parental strain in laborato...

journal_title：FEMS microbiology letters

authors： Malhotra V,Sharma D,Ramanathan VD,Shakila H,Saini DK,Chakravorty S,Das TK,Li Q,Silver RF,Narayanan PR,Tyagi JS

更新日期：2004-02-16 00:00:00

abstract：:Bacillus subtilis strains BS49 and BS34A, both derived from a common ancestor, carry one or more copies of Tn916, an extremely common mobile genetic element capable of transfer to and from a broad range of microorganisms. Here, we report the complete genome sequence of BS49 and the draft genome sequence of BS34A, whic...

journal_title：FEMS microbiology letters

authors： Browne HP,Anvar SY,Frank J,Lawley TD,Roberts AP,Smits WK

更新日期：2015-01-01 00:00:00

abstract：:In this study, 52 Streptococcus suis isolates from pigs in southern China were divided into four known sequence types (STs) and six new STs, using multilocus sequence typing. Ten representative isolates were selected from 10 STs for the analysis of whole genome sequences. Virulence was assessed in 10 isolates, which w...

journal_title：FEMS microbiology letters

authors： Peng L,Lin M,Huang Z,Guo S,Sun H,Yang X

更新日期：2020-03-01 00:00:00

abstract：:Growth of a Mycobacterium avium complex serotype 20 rough-colony variant in 1.0% Tween 80 resulted in a smooth-colony morphological conversion that was reversible upon removal of Tween and was not associated with the presence of serotype-specific glycopeptidolipid antigens. Electron microscopic examination suggested t...

journal_title：FEMS microbiology letters

authors： Wright EL,Pourshafie M,Barrow WW

更新日期：1992-11-01 00:00:00

abstract：:The ability of Candida albicans to adhere to subendothelial extracellular matrix (ECM) may be important in the pathogenesis of disseminated candidiasis. ECM proteins, such as fibronectin, laminin, and types I and IV collagen bind C. albicans avidly. These proteins all possess heparin-binding domains. The influence of ...

journal_title：FEMS microbiology letters

authors： Klotz SA,Smith RL

更新日期：1992-12-01 00:00:00