Abstract:
:A gene expression system for both Bacillus subtilis and Escherichia coli was developed. The expression vector, pHASH102, produces any combination of promoter and open reading frame to be expressed based on the T-extended cloning method. Because the pHASH series vectors are designed to shuttle between the genome and a high copy plasmid in B. subtilis, the expression profiles of copy number dependence can be examined systematically. We demonstrated that vectors with Pr, Pspac, and PS10 promoters are suitable for the overexpression of GFPuv. Moreover, aadK encoding aminoglycoside 6-adenylyltransferase (a streptomycin-resistance gene) of B. subtilis was successfully overexpressed in both B. subtilis and E. coli. These highly expressed GFPuv and aadK genes can be used as a genetic marker for both organisms.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Ohashi Y,Ohshima H,Tsuge K,Itaya Mdoi
10.1016/S0378-1097(03)00171-Xkeywords:
subject
Has Abstractpub_date
2003-04-11 00:00:00pages
125-30issue
1eissn
0378-1097issn
1574-6968pii
S037810970300171Xjournal_volume
221pub_type
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abstract::Amanita exitialis Zhu L. Yang and T.H. Li is a lethal mushroom species recently isolated in Guangdong Province, China. In this report, a pure culture of this species was obtained for the first time. To confirm the identity of the pure culture, the internal transcribed spacer regions of the nuclear ribosomal DNA of the...
journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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abstract::Primers designed to conserved regions of botulinum and tetanus clostridial toxins were used to amplify DNA fragments from non-proteolytic Clostridium botulinum type F (202F) DNA using polymerase chain reaction technology. The fragments were cloned and the complete nucleotide sequence of the gene encoding type F toxin ...
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journal_title:FEMS microbiology letters
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