Far different levels of gene expression provided by an oriented cloning system in Bacillus subtilis and Escherichia coli.

Abstract:

:A gene expression system for both Bacillus subtilis and Escherichia coli was developed. The expression vector, pHASH102, produces any combination of promoter and open reading frame to be expressed based on the T-extended cloning method. Because the pHASH series vectors are designed to shuttle between the genome and a high copy plasmid in B. subtilis, the expression profiles of copy number dependence can be examined systematically. We demonstrated that vectors with Pr, Pspac, and PS10 promoters are suitable for the overexpression of GFPuv. Moreover, aadK encoding aminoglycoside 6-adenylyltransferase (a streptomycin-resistance gene) of B. subtilis was successfully overexpressed in both B. subtilis and E. coli. These highly expressed GFPuv and aadK genes can be used as a genetic marker for both organisms.

journal_name

FEMS Microbiol Lett

authors

Ohashi Y,Ohshima H,Tsuge K,Itaya M

doi

10.1016/S0378-1097(03)00171-X

keywords:

subject

Has Abstract

pub_date

2003-04-11 00:00:00

pages

125-30

issue

1

eissn

0378-1097

issn

1574-6968

pii

S037810970300171X

journal_volume

221

pub_type

杂志文章
  • The pWW0 plasmid imposes a stochastic expression regime to the chromosomal ortho pathway for benzoate metabolism in Pseudomonas putida.

    abstract::Environmental plasmids often expand the metabolic repertoire of bacteria that carry them, but they also interfere with the biochemical and genetic network of the host. The pWW0 plasmid born by Pseudomonas putida mt-2 encodes the TOL pathway for degradation of toluene/m-xylene through production of intermediate compoun...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12400

    authors: Silva-Rocha R,de Lorenzo V

    更新日期:2014-07-01 00:00:00

  • Production of novel pullulanases at high concentrations by two newly isolated thermophilic clostridia.

    abstract::Two thermophilic bacteria, which are capable of growing on starch at 60-70 degrees C under anaerobic conditions, were isolated from a sugar refinery in Uelzen and from Solar lake in Israel. On the basis of their physiological characteristics they were identified as Clostridium thermohydrosulfuricum Uel 1 and C. thermo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90429-t

    authors: Klingeberg M,Hippe H,Antranikian G

    更新日期:1990-05-01 00:00:00

  • Attempts to revive Mycobacterium tuberculosis from 300-year-old human mummies.

    abstract::Environmental persistence of Mycobacterium tuberculosis is subject to speculation. However, the reality that infected postmortem tissues can be a danger to pathologists and embalmers has worrisome implications. A few experimental studies have demonstrated the organism's ability to withstand exposure to embalming fluid...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01150.x

    authors: Lemma E,Zimhony O,Greenblatt CL,Koltunov V,Zylber MI,Vernon K,Spigelman M

    更新日期:2008-06-01 00:00:00

  • Ability of human sera to neutralise the activity of Vero cytotoxins VT1, VT2 and variant forms of VT2.

    abstract::Sera, from 17 patients with diarrhoea or haemolytic uraemic syndrome, and six healthy adults, were tested for neutralisation of Vero cytotoxins (VT). For all 17 patients there was evidence of infection with Escherichia coli O157. Sera from two controls but from none of the patients neutralised VT1, although two patien...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb06652.x

    authors: Scotland SM,Said B,Thomas A,Rowe B

    更新日期:1994-01-15 00:00:00

  • Production and characterization of Amanitin toxins from a pure culture of Amanita exitialis.

    abstract::Amanita exitialis Zhu L. Yang and T.H. Li is a lethal mushroom species recently isolated in Guangdong Province, China. In this report, a pure culture of this species was obtained for the first time. To confirm the identity of the pure culture, the internal transcribed spacer regions of the nuclear ribosomal DNA of the...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.08.049

    authors: Zhang P,Chen Z,Hu J,Wei B,Zhang Z,Hu W

    更新日期:2005-11-15 00:00:00

  • Low-moisture food matrices as probiotic carriers.

    abstract::To exert a beneficial effect on the host, adequate doses of probiotics must be administered and maintaining their viability until consumption is thus essential. Dehydrated probiotics exhibit enhanced long-term viability and can be incorporated into low-moisture food matrices, which also possess high stability at refri...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fnz006

    authors: Marcial-Coba MS,Knøchel S,Nielsen DS

    更新日期:2019-01-01 00:00:00

  • Efficient screening for astaxanthin-overproducing mutants of the yeast Xanthophyllomyces dendrorhous by flow cytometry.

    abstract::Astaxanthin possesses higher antioxidant activity than other carotenoids and, for this and other reasons, has great commercial potential for use in the aquaculture, pharmaceutical, and food industries. The basidiomycetous yeast Xanthophyllomyces dendrorhous is one of the best natural producers of astaxanthin, but wild...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01278.x

    authors: Ukibe K,Katsuragi T,Tani Y,Takagi H

    更新日期:2008-09-01 00:00:00

  • The antibacterial effects of terpene alcohols on Staphylococcus aureus and their mode of action.

    abstract::The study was made of the antibacterial effects of three terpene alcohols on Staphylococcus aureus, focusing on the leakage of K+ ions and toxicity over time. The leakage of K+ ions was monitored continuously with a K+-electrode. Our results suggested that the terpene alcohols, namely, farnesol, nerolidol and plaunoto...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.06.049

    authors: Inoue Y,Shiraishi A,Hada T,Hirose K,Hamashima H,Shimada J

    更新日期:2004-08-15 00:00:00

  • 7-fluoroindole as an antivirulence compound against Pseudomonas aeruginosa.

    abstract::The emergence of antibiotic resistance has necessitated new therapeutic approaches for combating persistent bacterial infection. An alternative approach is regulation of bacterial virulence instead of growth suppression, which can readily lead to drug resistance. The virulence of the opportunistic human pathogen Pseud...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2012.02500.x

    authors: Lee JH,Kim YG,Cho MH,Kim JA,Lee J

    更新日期:2012-04-01 00:00:00

  • The capsular turnover product of Staphylococcus aureus strain Smith.

    abstract::The capsular polysaccharide released from the bacterial surface by cell wall turnover during growth exhibited less size heterogeneity and a higher average molecular mass than the polysaccharide extracted from the cell by treatment with lysostaphin or low pH. Treatment of turnover polysaccharide, radiolabelled by growt...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb06800.x

    authors: Havaei SA,Hancock IC

    更新日期:1994-05-01 00:00:00

  • Cloning and characterization of a novel Cry1A toxin from Bacillus thuringiensis with high toxicity to the Asian corn borer and other lepidopteran insects.

    abstract::A novel cry1A was cloned from Bacillus thuringiensis strain BT8 and expressed in the B. thuringiensis acrystalliferous mutant HD73(-). The gene, designated cry1Ah1, encoded a protein with a molecular weight of 134 kDa. Reverse transcriptase-PCR and Western blotting showed that Cry1Ah was expressed in the host strain B...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.01053.x

    authors: Xue J,Liang G,Crickmore N,Li H,He K,Song F,Feng X,Huang D,Zhang J

    更新日期:2008-03-01 00:00:00

  • Characterization of the gltF gene product of Escherichia coli.

    abstract::The glt operon of Escherichia coli comprises the structural genes for the glutamate synthase subunits (gltB and gltD) and gltF, whose product was previously suggested to have regulatory functions. The A/T-rich region between gltD and gltF contains a weak promoter and a translation initiation site for gltF. The GltF pr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb08711.x

    authors: Grassl G,Bufe B,Müller B,Rösel M,Kleiner D

    更新日期:1999-10-01 00:00:00

  • Neuraminidase (sialidase) activity of Haemophilus parasuis.

    abstract::Neuraminidase (sialidase), a potential virulence factor in bacteria, was demonstrated in Haemophilus parasuis, an invasive swine pathogen, but not in four other pathogens of the Pasteurellaceae family: H. influenzae, H. somnus, H. paragallinarum, or Actinobacillus pleuropneumoniae. H. parasuis neuraminidase had an aci...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1997.tb10438.x

    authors: Lichtensteiger CA,Vimr ER

    更新日期:1997-07-15 00:00:00

  • CpaA a novel protease from Acinetobacter baumannii clinical isolates deregulates blood coagulation.

    abstract::Acinetobacter baumannii is an important nosocomial pathogen that displays high antibiotic resistance. It causes a variety of infections including pneumonias and sepsis which may result in disseminated intravascular coagulation. In this work, we identify and characterize a novel secreted, zinc-dependent, metallo-endope...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12496

    authors: Tilley D,Law R,Warren S,Samis JA,Kumar A

    更新日期:2014-07-01 00:00:00

  • Sphingomonas astaxanthinifaciens sp. nov., a novel astaxanthin-producing bacterium of the family Sphingomonadaceae isolated from Misasa, Tottori, Japan.

    abstract::A red-pigmented, Gram-negative, motile, strictly aerobic, mesophilic, oval- or short rod-shaped bacterium (TDMA-17(T)) was isolated from fresh water collected at Misasa, a radioactive site in Japan. TDMA-17(T) was slightly tolerant against gamma-ray irradiation, and effectively produced carotenoids (2.8 mg g(-1) dry c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00760.x

    authors: Asker D,Beppu T,Ueda K

    更新日期:2007-08-01 00:00:00

  • tRNA genes were found in Piscirickettsia salmonis 16S-23S rDNA spacer region (ITS).

    abstract::Piscirickettsia salmonis is the etiological agent of Salmonid Rickettsial Septicemia, a disease affecting salmon aquaculture industry. We analyzed the 16S-23S rDNA spacer region (internal transcribed spacer, ITS) of Chilean P. salmonis isolates LF-89 and EM-90. Two main ITS amplification products were obtained by PCR ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10576.x

    authors: Casanova A,Obreque J,Sandino AM,Jashés M

    更新日期:2001-04-01 00:00:00

  • Sequence of the gene encoding type F neurotoxin of Clostridium botulinum.

    abstract::Primers designed to conserved regions of botulinum and tetanus clostridial toxins were used to amplify DNA fragments from non-proteolytic Clostridium botulinum type F (202F) DNA using polymerase chain reaction technology. The fragments were cloned and the complete nucleotide sequence of the gene encoding type F toxin ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90408-g

    authors: East AK,Richardson PT,Allaway D,Collins MD,Roberts TA,Thompson DE

    更新日期:1992-09-15 00:00:00

  • bkaR is a TetR-type repressor that controls an operon associated with branched-chain keto-acid metabolism in Mycobacteria.

    abstract::This study describes how bkaR, a highly conserved mycobacterial TetR-like transcriptional repressor, regulates a number of nearby genes that have associations with branched-chain keto-acid metabolism. bkaR (MSMEG_4718) was deleted from the nonpathogenic species Mycobacterium smegmatis, and changes in global gene expre...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/1574-6968.12196

    authors: Balhana RJ,Swanston SN,Coade S,Withers M,Sikder MH,Stoker NG,Kendall SL

    更新日期:2013-08-01 00:00:00

  • Board game versus lecture-based seminar in the teaching of pharmacology of antimicrobial drugs--a randomized controlled trial.

    abstract::The effectiveness of an educational board game developed to teach the pharmacology of antimicrobial drugs to medical students was compared with the lecture-based seminar as a supplemental tool to improve short- and long-term knowledge retention and the perception of the learning method by students. A group of 124 stud...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,随机对照试验

    doi:10.1093/femsle/fnw045

    authors: Karbownik MS,Wiktorowska-Owczarek A,Kowalczyk E,Kwarta P,Mokros Ł,Pietras T

    更新日期:2016-04-01 00:00:00

  • Polymerase chain reaction for identification of human and porcine spirochaetes recovered from cases of intestinal spirochaetosis.

    abstract::A polymerase chain reaction (PCR) amplification of 16S rDNA was developed to identify spirochaetes recovered from cases of intestinal spirochaetosis in humans and pigs; these bacteria belong to a distinct genetic group of spirochaetes, with the proposed name 'Anguillina coli'. The PCR incorporated a universal eubacter...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07362.x

    authors: Park NY,Chung CY,McLaren AJ,Atyeo RF,Hampson DJ

    更新日期:1995-01-15 00:00:00

  • Detection of Nitrosomonas spp. by polymerase chain reaction.

    abstract::A unique genomic DNA fragment was isolated from Nitrosomonas europaea ATCC 19718. Based on the sequence of this fragment, oligonucleotide primers for polymerase chain reaction amplification were prepared which amplify sequences of 775 and 658 bp. The predicted DNA fragments were both amplified from the genome of N. eu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb07029.x

    authors: Nejidat A,Abeliovich A

    更新日期:1994-07-01 00:00:00

  • Beyond the single answer: a process-oriented exam for science students.

    abstract::This commentary describes an assessment exercise known as the TRIPSE (Tri-Partite Problem Solving Exercise) that mimics science in operation. Students frame hypotheses based on limited data, design experiments to test them, which they later revise with new information. It is emphasised that there are no single correct...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnw176

    authors: Nastos S,Rangachari PK

    更新日期:2016-08-01 00:00:00

  • Ku70 or Ku80 deficiencies in the fungus Botrytis cinerea facilitate targeting of genes that are hard to knock out in a wild-type context.

    abstract::The filamentous ascomycete Botrytis cinerea is one of the most studied models for understanding the necrotrophic behaviour of phytopathogenic fungi. The genomes of two strains of B. cinerea have been sequenced (B05.10 and T4), which may contribute to elucidating the virulence polymorphism in this fungus. In this study...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01388.x

    authors: Choquer M,Robin G,Le Pêcheur P,Giraud C,Levis C,Viaud M

    更新日期:2008-12-01 00:00:00

  • Inactivation of the petE gene encoding plastocyanin causes different photosynthetic responses in cyanobacterium Synechocystis PCC 6803 under light-dark photoperiod and continuous light conditions.

    abstract::Plastocyanin, encoded by the petE gene, can transfer electrons to photosystem I (PSI) and cytochrome c oxidase during photosynthetic and respiratory metabolism in cyanobacteria. We constructed a petE mutant of Synechocystis sp. strain PCC 6803 and investigated its phenotypic properties under different light conditions...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/1574-6968.12101

    authors: Wang XQ,Jiang HB,Zhang R,Qiu BS

    更新日期:2013-04-01 00:00:00

  • Aerobic and anaerobic nitrate and nitrite reduction in free-living cells of Bradyrhizobium sp. (Lupinus).

    abstract::Induction, energy gain, effect on growth, and interaction of nitrate and nitrite reduction of Bradyrhizobium sp. (Lupinus) USDA 3045 were characterized. Both nitrate and nitrite were reduced in air, although nitrite reduction was insensitive to ammonium inhibition. Anaerobic reduction of both ions was shown to be link...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00620-7

    authors: Polcyn W,Luciński R

    更新日期:2003-09-26 00:00:00

  • Antifungal activity of three spermidine conjugates.

    abstract::Three tri-substituted spermidines, di-p-coumaroyl-caffeoylspermidine, tri-caffeoylspermidine and tri-p-coumaroylspermidine, isolated from pollen of Quercus alba, were examined for antifungal activity. Both di-p-coumaroyl-caffeoylspermidine and tri-p-coumaroylspermidine reduced mycelial growth of the oat leaf stripe pa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10765.x

    authors: Walters D,Meurer-Grimes B,Rovira I

    更新日期:2001-07-24 00:00:00

  • Mechanisms of protein kinase Sch9 regulating Bcy1 in Saccharomyces cerevisiae.

    abstract::In this study, we investigated the mechanisms of Sch9 regulating the localization and phosphorylation of Bcy1. Our research indicated that Sch9 regulated localization of Bcy1 via Zds1 for the following reasons: (1) deletions of SCH9 or ZDS1 both caused nuclear localization of Bcy1; (2) Sch9 and Zds1 interacted physica...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2012.02552.x

    authors: Zhang A,Gao W

    更新日期:2012-06-01 00:00:00

  • Successful enrichment of low-abundant comammox Nitrospira from nitrifying granules under ammonia-limited conditions.

    abstract::In artificial engineered systems, nitrification is a key reaction that accounts for the removal of biological nitrogen. Recently, a single microbe capable of oxidizing ammonia to nitrate, known as a complete ammonia oxidizer (comammox), has been discovered. Although the abundance and diversity of comammox Nitrospira i...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnaa025

    authors: Fujitani H,Nomachi M,Takahashi Y,Hasebe Y,Eguchi M,Tsuneda S

    更新日期:2020-01-01 00:00:00

  • Lactobacillus fermentum 3872 genome sequencing reveals plasmid and chromosomal genes potentially involved in a probiotic activity.

    abstract::In this report we describe a Lactobacillus fermentum 3872 plasmid (pLF3872) not previously found in any other strain of this species. The analysis of the complete sequence of this plasmid revealed the presence of a gene encoding a large collagen-binding protein (CBP), as well as the genes responsible for plasmid maint...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv068

    authors: Lehri B,Seddon AM,Karlyshev AV

    更新日期:2015-06-01 00:00:00

  • Stable carbon isotope fractionations of the hyperthermophilic crenarchaeon Metallosphaera sedula.

    abstract::The stable carbon isotopic compositions of the inorganic carbon source, bulk cell material, and isoprenoid lipids of the hyperthermophilic crenarchaeon Metallosphaera sedula, which uses a 3-hydroxypropionate-like pathway for autotrophic carbon fixation, have been measured. Bulk cell material was approximately 3 per th...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10542.x

    authors: van der Meer MT,Schouten S,Rijpstra WI,Fuchs G,Sinninghe Damsté JS

    更新日期:2001-03-01 00:00:00