Sequence of the gene encoding type F neurotoxin of Clostridium botulinum.

Abstract:

:Primers designed to conserved regions of botulinum and tetanus clostridial toxins were used to amplify DNA fragments from non-proteolytic Clostridium botulinum type F (202F) DNA using polymerase chain reaction technology. The fragments were cloned and the complete nucleotide sequence of the gene encoding type F toxin determined. Analysis of the nucleotide sequence demonstrated the presence of an open frame encoding a protein of 1274 amino acids, similar to other botulinum neurotoxins. Upstream of the toxin gene is the end of an open reading frame which encodes the C-terminus of a protein with homology to non-toxic-non-hemagglutinin component of type C progenitor toxin.

journal_name

FEMS Microbiol Lett

authors

East AK,Richardson PT,Allaway D,Collins MD,Roberts TA,Thompson DE

doi

10.1016/0378-1097(92)90408-g

keywords:

subject

Has Abstract

pub_date

1992-09-15 00:00:00

pages

225-30

issue

2-3

eissn

0378-1097

issn

1574-6968

journal_volume

75

pub_type

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