Abstract:
:Primers designed to conserved regions of botulinum and tetanus clostridial toxins were used to amplify DNA fragments from non-proteolytic Clostridium botulinum type F (202F) DNA using polymerase chain reaction technology. The fragments were cloned and the complete nucleotide sequence of the gene encoding type F toxin determined. Analysis of the nucleotide sequence demonstrated the presence of an open frame encoding a protein of 1274 amino acids, similar to other botulinum neurotoxins. Upstream of the toxin gene is the end of an open reading frame which encodes the C-terminus of a protein with homology to non-toxic-non-hemagglutinin component of type C progenitor toxin.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
East AK,Richardson PT,Allaway D,Collins MD,Roberts TA,Thompson DEdoi
10.1016/0378-1097(92)90408-gkeywords:
subject
Has Abstractpub_date
1992-09-15 00:00:00pages
225-30issue
2-3eissn
0378-1097issn
1574-6968journal_volume
75pub_type
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