Production and characterization of Amanitin toxins from a pure culture of Amanita exitialis.

abstract：:The effect of low concentrations of cyanide on dissimilatory perchlorate and chlorate reduction and aerobic respiration was examined using pure cultures of Azospira sp. KJ. Cyanide at a concentration of 38 microM inhibited cell growth on perchlorate, chlorate and molecular oxygen, but it did not inhibit the activity o...

journal_title：FEMS microbiology letters

authors： Song Y,Logan BE

更新日期：2004-10-15 00:00:00

abstract：:Cholera epidemics caused by Vibrio cholerae 01 continue to represent a major public health concern in many developing countries. A rapid and simple test kit for the detection of V. cholerae 01 has been developed. The kit, CholeraScreen is a monoclonal antibody-based, co-agglutination test and is used directly with sto...

journal_title：FEMS microbiology letters

authors： Colwell RR,Hasan JA,Huq A,Loomis L,Siebeling RJ,Torres M,Galvez S,Islam S,Tamplin MT,Bernstein D

更新日期：1992-10-15 00:00:00

abstract：:The transport of radioactively labelled uracil into submerged mycelium of T. viride was measured by means of a membrane filtration technique. It was found to be time-dependent (up to 90 min) and concentration-dependent (up to 8 mmol l-1). Its concentration dependence was biphasic and consisted from the saturatable par...

journal_title：FEMS microbiology letters

authors： Lakatos B,Simkovic M,Betina V,Varecka L

更新日期：1999-02-15 00:00:00

abstract：:micF RNA, whose sequence is highly complementary to a 5'-portion of ompF mRNA, has been implicated in the osmoregulation and thermoregulation of the ompF porin gene in Escherichia coli. To define and characterize cis-acting regulatory regions upstream of the micF promoter, a series of deletions of the micF promoter fu...

journal_title：FEMS microbiology letters

authors： Takayanagi K,Maeda S,Mizuno T

更新日期：1991-09-15 00:00:00

abstract：:A 1.8-kb cryptic plasmid pFR18 was isolated from Leuconostoc mesenteroides ssp. mesenteroides FR52 and characterized. The identification of single-stranded DNA intermediate (ssDNA) in Leuconostoc demonstrated that the replication of pFR18 is directed by a rolling-circle mechanism (RCR). Sequence analysis revealed a si...

journal_title：FEMS microbiology letters

authors： Biet F,Cenatiempo Y,Fremaux C

更新日期：1999-10-15 00:00:00

abstract：:Ruminococcus albus is a Gram-positive bacterium that degrades plant cell walls in the rumen of herbivores. It was described to synthesize two major glycoside-hydrolases (Cel9B and Cel48A), which are exported and anchored at the cell surface. In bacteria, proteins destined to cross the cytoplasmic membrane are synthesi...

journal_title：FEMS microbiology letters

authors： Esbelin J,Martin C,Forano E,Mosoni P

更新日期：2009-05-01 00:00:00

abstract：:We have studied the intraperoxisomal location of catalase in peroxisomes of methanol-grown Hansenula polymorpha by (immuno)cytochemical means. In completely crystalline peroxisomes, in which the crystalline matrix is composed of octameric alcohol oxidase (AO) molecules, most of the catalase protein is located in a nar...

journal_title：FEMS microbiology letters

authors： Keizer I,Roggenkamp R,Harder W,Veenhuis M

更新日期：1992-05-15 00:00:00

abstract：:This study investigated 247 Escherichia coli isolates collected from four cattle farms to characterize aminoglycoside-modifying enzyme (AME) genes, their plasmid replicons and transferability. Out of 247 isolates a high number of isolates (total 202; 81.78%) were found to be resistant to various antibiotics by disc di...

journal_title：FEMS microbiology letters

authors： Belaynehe KM,Shin SW,Hong-Tae P,Yoo HS

更新日期：2017-08-01 00:00:00

abstract：:An iterative position-specific score matrix (PSSM)-based approach was used to predict sigma(28) promoters in 11 Shewanella genomes. The Shewanella Correlation Browser was used to distinguish true-positive predictions from false-positive predictions in Shewanella oneidensis MR-1 by generating a sigma(28)-regulated tran...

journal_title：FEMS microbiology letters

authors： Song W,Juhn FS,Naiman DQ,Konstantinidis KT,Gardner TS,Ward MJ

更新日期：2008-06-01 00:00:00

abstract：:The gene encoding the alpha-subunit of methanol dehydrogenase (mxaF) and its flanking region was isolated from a methylotrophic bacterium, Hyphomicrobium methylovorum GM2. The deduced amino acid sequence of MxaF showed 80, 80, 74 and 66% identity with those of Methylobacterium extorquens AM1, M. organophilum XX, Parac...

journal_title：FEMS microbiology letters

authors： Tanaka Y,Yoshida T,Watanabe K,Izumi Y,Mitsunaga T

更新日期：1997-09-15 00:00:00

abstract：:Molecular information suggests that there is a broad diversity of acetogens in the rumen, distinct from any currently isolated acetogens. We combined molecular analysis with enrichment culture techniques to investigate this diversity further. Methane-inhibited, hydrogenotrophic enrichment cultures produced acetate as ...

journal_title：FEMS microbiology letters

authors： Gagen EJ,Padmanabha J,Denman SE,McSweeney CS

更新日期：2015-07-01 00:00:00

abstract：:Five mutations in ccpA of Bacillus megaterium with impaired functions were analysed for carbon catabolite repression. The phenotypes support the hypothesis that CcpA assumes a PurR/LacI fold. The completely inactive mutants CcpA119GE and CcpA326am cause alterations which are incompatible with that fold. A mutation wit...

journal_title：FEMS microbiology letters

authors： Kraus A,Hillen W

更新日期：1997-08-01 00:00:00

abstract：:Trehalase was studied in Schizosaccharomyces pombe cells growing vegetatively on minimal medium and in sporulating cultures. Acid trehalase activity, measured at pH 4.2, was absent in vegetative cells and occurred only in asci, indicating that this activity represented the sporulation-specific trehalase reported previ...

journal_title：FEMS microbiology letters

authors： De Virgilio C,Müller J,Boller T,Wiemken A

更新日期：1991-11-01 00:00:00

abstract：:Haemophilus influenzae has an absolute growth requirement for heme and a heme binding lipoprotein (HbpA) has been implicated in the utilization of this essential nutrient. HbpA was identified by examining clones from an H. influenzae genomic library that caused Escherichia coli harboring the clone to bind heme. Howeve...

journal_title：FEMS microbiology letters

authors： Morton DJ,Madore LL,Smith A,Vanwagoner TM,Seale TW,Whitby PW,Stull TL

更新日期：2005-12-15 00:00:00

abstract：:We have identified, cloned and characterized a formerly unknown protein from Streptomyces lividans spores. The deduced protein belongs to a novel member of the metallophosphatase superfamily and contains a phosphatase domain and predicted binding sites for divalent ions. Very close relatives are encoded in the genomic...

journal_title：FEMS microbiology letters

authors： Lamp J,Weber M,Cingöz G,Ortiz de Orué Lucana D,Schrempf H

更新日期：2013-05-01 00:00:00

abstract：:The Gram-positive bacterium Streptococcus gordonii has been genetically engineered to allow the simultaneous expression of two heterologous proteins at the cell surface. A family of recombinant streptococci displaying two different antigens was constructed. All the strains were genetically stable and expressed both pr...

journal_title：FEMS microbiology letters

authors： Maggi T,Spinosa M,Ricci S,Medaglini D,Pozzi G,Oggioni MR

更新日期：2002-04-23 00:00:00

abstract：:pH is a key environmental factor affecting the physiology, ecology and pathogenicity of the oral biofilms colonizing the hard tissues of the human mouth. Much attention has been focused on the production of organic acids through the metabolism of carbohydrates by pathogenic oral bacteria. Now, evidence is emerging tha...

journal_title：FEMS microbiology letters

authors： Burne RA,Marquis RE

更新日期：2000-12-01 00:00:00

abstract：:The variable stress-sensitivity of individual cells within pure cultures is widely noted but generally unexplained. Here, factors determining the heterogeneous susceptibility to copper toxicity in Saccharomyces cerevisiae were examined with a rapid non-perturbing approach based on flow cytometry. By determination of t...

journal_title：FEMS microbiology letters

authors： Howlett NG,Avery SV

更新日期：1999-07-15 00:00:00

abstract：:To investigate the regulatory mechanism governing antifungal metabolite biosynthesis, two kinds of global regulator genes in Pseudomonas sp. M18, an rpoS and an rsmA gene, were cloned and mutated by inserting with an aacC1 cassette, respectively. Two mutants showed the same regulatory mode with repression of phenazine...

journal_title：FEMS microbiology letters

authors： Ge Y,Yang S,Fang Y,Yang R,Mou D,Cui J,Wen L

更新日期：2007-03-01 00:00:00

abstract：:Amylosucrase produces an insoluble alpha-1,4-linked glucan from sucrose, releasing fructose. In addition to polymerisation, in the presence of sucrose as sole substrate, amylosucrase catalyses sucrose hydrolysis and oligosaccharide synthesis in significant proportions. The effects of both glycogen acceptor and sucrose...

journal_title：FEMS microbiology letters

authors： Potocki de Montalk G,Remaud-Simeon M,Willemot RM,Monsan P

更新日期：2000-05-01 00:00:00

abstract：:Members of the Streptococcus bovis group are frequent colonizers of the intestinal tract, which can also cause endocarditis. However, their ability to adhere to and colonize host tissues and the factors associated with pathogenicity are largely unknown. Here, we assessed 17 endocarditis-derived human isolates [identif...

journal_title：FEMS microbiology letters

authors： Sillanpää J,Nallapareddy SR,Singh KV,Ferraro MJ,Murray BE

更新日期：2008-12-01 00:00:00

abstract：:Dimethylphthalate (DMP), one of the phthalate esters, is used in the manufacture of plasticizers, insect repellents, and synthetic fibers, and contributes to environmental pollution. In the present study, we report a novel bacterium belonging to the Bacillus sp., which has the ability to utilize DMP as the sole source...

journal_title：FEMS microbiology letters

authors： Niazi JH,Prasad DT,Karegoudar TB

更新日期：2001-03-15 00:00:00

abstract：:An open reading frame located in the tyrB-uvrA intergenic region of the Escherichia coli MG1655 chromosome was identified as encoding the class B acid phosphatase of this species on the basis of cloning and expression experiments. A protocol for purification of the enzyme (named AphA) was developed, and its properties...

journal_title：FEMS microbiology letters

authors： Thaller MC,Schippa S,Bonci A,Cresti S,Rossolini GM

更新日期：1997-01-15 00:00:00

abstract：:The genus Pycnoporus forms a group of four species known especially for producing high redox potential laccases suitable for white biotechnology. A sample of 36 Pycnoporus strains originating from different geographical areas was studied to seek informative molecular markers for the typing of new strains in laboratory...

journal_title：FEMS microbiology letters

authors： Lesage-Meessen L,Haon M,Uzan E,Levasseur A,Piumi F,Navarro D,Taussac S,Favel A,Lomascolo A

更新日期：2011-12-01 00:00:00

abstract：:The genome of Helicobacter pylori 26695 has been sequenced and the lipopolysaccharide (LPS) O sidechain of this strain has been shown to express both Lewis x and Lewis y units. To determine the role of HP0159 and HP1416, genes recognized as rfaJ homologs and implicated in LPS synthesis, isogenic mutants of H. pylori 2...

journal_title：FEMS microbiology letters

authors： Moran AP,Shiberu B,Ferris JA,Knirel YA,Senchenkova SN,Perepelov AV,Jansson PE,Goldberg JB

更新日期：2004-12-01 00:00:00

abstract：:The objective of this work was to analyze the effects of thermosonication (TS) on induction of a viable but non-culturable (VBNC) state in Salmonella Typhimurium and to examine incubation factors affecting subsequent resuscitation of cells. A TS treatment of 380 W at 53°C for 30 min induced the VBNC state in S. Typhim...

journal_title：FEMS microbiology letters

authors： Liao H,Jiang L,Zhang R

更新日期：2018-02-01 00:00:00

abstract：:The PenJ protein of the penicillinase gene (penP) expression system from Bacillus licheniformis is an antirepressor and membrane receptor for beta-lactam antibiotics. A putative beta-lactam antibiotic binding site including Ser402 and Lys405, which are homologous to the conserved sequence for the beta-lactam binding s...

journal_title：FEMS microbiology letters

authors： Takagi M,Ohta T,Johki S,Imanaka T

更新日期：1993-06-01 00:00:00

abstract：:Abstract The emergence of the nontuberculosis mycobacteria (NTM) as clinically relevant pathogens has warranted the study of these ubiquitous organisms in the context of their likely environmental niche, the biofilm. We assayed the NTM bacterium Mycobacterium marinum strain 1218R, a fish outbreak isolate, for biofilm ...

journal_title：FEMS microbiology letters

authors： Hall-Stoodley L,Brun OS,Polshyna G,Barker LP

更新日期：2006-04-01 00:00:00

abstract：:Bordetella pertussis strains contain at least three distinct genes coding for fimbrial subunits, designated fim2, fim3, and fimX. The sequences of the fim2 and fimX genes have been published. Here we present the sequence of the fim3 gene. Proximal and distal to the fim3 gene, regions were observed that could function ...

journal_title：FEMS microbiology letters

authors： Mooi FR,ter Avest A,van der Heide HG

更新日期：1990-01-01 00:00:00

abstract：:Bacteria carry a number of genes that cause cell growth arrest or cell lysis upon expression. Notably, defective prophages retain many lysis proteins. Here, we identified a novel lytic gene, ydfD, on the Qin prophage segment of the Escherichia coli genome. YdfD lyses 99.9% of cells within 2 h of its induction. The co-...

journal_title：FEMS microbiology letters

authors： Masuda H,Awano N,Inouye M

更新日期：2016-03-01 00:00:00