Abstract:
:An open reading frame located in the tyrB-uvrA intergenic region of the Escherichia coli MG1655 chromosome was identified as encoding the class B acid phosphatase of this species on the basis of cloning and expression experiments. A protocol for purification of the enzyme (named AphA) was developed, and its properties were analyzed. The enzyme is a 100-kDa homotetrameric protein which apparently requires a metal co-factor for activity. Similarly to other bacterial class B acid phosphatases, it is able to dephosphorylate several organic phosphomonoesters as well as to catalyze the transfer of low-energy phosphate groups from phosphomonoesters to hydroxyl groups of various organic compounds.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Thaller MC,Schippa S,Bonci A,Cresti S,Rossolini GMdoi
10.1111/j.1574-6968.1997.tb10192.xsubject
Has Abstractpub_date
1997-01-15 00:00:00pages
191-8issue
2eissn
0378-1097issn
1574-6968pii
S0378-1097(96)00474-0journal_volume
146pub_type
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