Identification of the gene (aphA) encoding the class B acid phosphatase/phosphotransferase of Escherichia coli MG1655 and characterization of its product.

Abstract:

:An open reading frame located in the tyrB-uvrA intergenic region of the Escherichia coli MG1655 chromosome was identified as encoding the class B acid phosphatase of this species on the basis of cloning and expression experiments. A protocol for purification of the enzyme (named AphA) was developed, and its properties were analyzed. The enzyme is a 100-kDa homotetrameric protein which apparently requires a metal co-factor for activity. Similarly to other bacterial class B acid phosphatases, it is able to dephosphorylate several organic phosphomonoesters as well as to catalyze the transfer of low-energy phosphate groups from phosphomonoesters to hydroxyl groups of various organic compounds.

journal_name

FEMS Microbiol Lett

authors

Thaller MC,Schippa S,Bonci A,Cresti S,Rossolini GM

doi

10.1111/j.1574-6968.1997.tb10192.x

subject

Has Abstract

pub_date

1997-01-15 00:00:00

pages

191-8

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(96)00474-0

journal_volume

146

pub_type

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