Genetic engineering of Streptococcus gordonii for the simultaneous display of two heterologous proteins at the bacterial surface.


:The Gram-positive bacterium Streptococcus gordonii has been genetically engineered to allow the simultaneous expression of two heterologous proteins at the cell surface. A family of recombinant streptococci displaying two different antigens was constructed. All the strains were genetically stable and expressed both proteins at the surface of the same bacterial cell. S. gordonii co-expressing the immunomodulating molecule LTB (B monomer of Escherichia coli heat-labile toxin) and the V3 domain of HIV-1 gp120 were inoculated subcutaneously to BALB/c mice. Animals were capable of responding to both antigens, producing LTB- and V3-specific serum IgG. The V3-specific IgG titer was four-fold higher in mice immunised with the double protein-expressing bacteria, as compared to control animals inoculated either with S. gordonii expressing the V3 domain alone or with a mixture of the two strains expressing LTB and V3, separately. Therefore, LTB was able to potentiate the antibody response towards the V3 domain, and this effect was observed only when LTB was co-expressed on the same bacterial cell.


FEMS Microbiol Lett


Maggi T,Spinosa M,Ricci S,Medaglini D,Pozzi G,Oggioni MR





Has Abstract


2002-04-23 00:00:00














  • Genetic variation among Mycoplasma agalactiae isolates detected by the variant surface lipoprotein gene (vspA) of Mycoplasma bovis.

    abstract::Multiple restriction fragments, homologous to the previously described Mycoplasma bovis vspA gene, were identified in the chromosome of Mycoplasma agalactiae. The vspA, a representative variable surface lipoprotein gene of the vsp gene family, and four synthetic oligonucleotides, representing sequences complementary t...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Flitman-Tene R,Levisohn S,Rosenbusch R,Rapoport E,Yogev D

    更新日期:1997-11-01 00:00:00

  • Nucleotide sequence and analysis of the gene in Borrelia burgdorferi encoding the immunogenic P39 antigen.

    abstract::The P39 antigen is a specific, highly conserved, and immunogenic protein of Lyme disease spirochetes, Borrelia burgdorferi sensu lato. The nucleotide sequence of the gene encoding this protein was determined and found to be the first of two tandemly arranged open reading frames located on the spirochete's chromosome. ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Simpson WJ,Cieplak W,Schrumpf ME,Barbour AG,Schwan TG

    更新日期:1994-06-15 00:00:00

  • The triazine hydrolase gene trzN from Nocardioides sp. strain C190: cloning and construction of gene-specific primers.

    abstract::Using oligonucleotides derived from the N-terminal sequence of a triazine hydrolase from Nocardioides sp. strain C190, two DNA fragments containing trzN were cloned into Escherichia coli and their nucleotide sequences were determined. The 456-amino acid polypeptide predicted from the 1356-bp trzN ORF displayed signifi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Mulbry WW,Zhu H,Nour SM,Topp E

    更新日期:2002-01-02 00:00:00

  • Growth inhibition of Bacteroides fragilis by hemopexin: proteolytic degradation of hemopexin to overcome heme limitation.

    abstract::The stimulatory effect of heme on growth of Bacteroides fragilis, an anaerobic human pathogen, was strongly inhibited by hemopexin, an avid (Kd<1 pM) heme-binding plasma protein. Both rabbit and human hemopexins were bacteriostatic for a limited period of time, suggesting an adaptation by B. fragilis to heme-limited g...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Rocha ER,Smith A,Smith CJ,Brock JH

    更新日期:2001-05-15 00:00:00

  • Characterisation of integrons and antibiotic resistance genes in Danish multiresistant Salmonella enterica Typhimurium DT104.

    abstract::The presence and genetic content of integrons was investigated in eight Salmonella enteritica Typhimurium DT104 isolates from different pig herds in Denmark. Two different integrons were identified using PCR and sequencing. Each of the integrons carried a single resistance cassette in addition to the sull and qacE del...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sandvang D,Aarestrup FM,Jensen LB

    更新日期:1998-03-01 00:00:00

  • A new subtype of the metalloprotease toxin gene and the incidence of the three bft subtypes among Bacteroides fragilis isolates in Japan.

    abstract::The bft gene encoding Bacteroides fragilis toxin (BFT) has been devided into two subtypes, bft-1 and bft-2. We found a novel subtype by sequencing a segment of the bft gene from 64 enterotoxigenic B. fragilis (ETBF) strains isolated in Japan. The 1548-bp nucleotide sequences of the new bft, the bft-1, and bft-2 genes ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kato N,Liu CX,Kato H,Watanabe K,Tanaka Y,Yamamoto T,Suzuki K,Ueno K

    更新日期:2000-01-01 00:00:00

  • Pyrimidine scavenging by Mycobacterium leprae.

    abstract::Mycobacterium leprae incorporated exogenously supplied pyrimidines as bases or nucleosides, but not as a nucleotide, into its nucleic acids. Notably, thymine was incorporated approximately 5 times more rapidly than thymidine by both suspensions of, or intracellular M. leprae. Thymine incorporation was significantly in...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wheeler PR

    更新日期:1989-01-15 00:00:00

  • Isolation of Rhodococcus rhodochrous NCIMB13064 derivatives with new biodegradative abilities.

    abstract::Rhodococcus rhodochrous NCIMB13064 can dehalogenate and utilise a number of halogenated aliphatic compounds as sole carbon and energy source. Mutants of NCIMB13064 can be easily isolated with an enlarged range of 1-chloroalkane utilising ability. Dehalogenation of 1-chlorononane, 1-chlorodecane and short-chain 1-chlor...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kulakova AN,Reid KA,Larkin MJ,Allen CC,Kulakov LA

    更新日期:1996-12-01 00:00:00

  • Anaerobic degradation of protocatechuate (3,4-dihydroxybenzoate) by Thauera aromatica strain AR-1.

    abstract::The denitrifying bacterium Thauera aromatica strain AR-1 grows anaerobically with protocatechuate (3,4-dihydroxybenzoate (DHB)) as sole energy and carbon source. This bacterium harbors two distinct pathways for degradation of aromatic compounds, the benzoyl-coenzyme A (CoA) pathway for benzoate degradation and the hyd...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Philipp B,Kemmler D,Hellstern J,Gorny N,Caballero A,Schink B

    更新日期:2002-06-18 00:00:00

  • Genomic suppression subtractive hybridization as a tool to identify differences in mycorrhizal fungal genomes.

    abstract::Characterization of genomic variation among different microbial species, or different strains of the same species, is a field of significant interest with a wide range of potential applications. We have investigated the genomic variation in mycorrhizal fungal genomes through genomic suppressive subtractive hybridizati...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Murat C,Zampieri E,Vallino M,Daghino S,Perotto S,Bonfante P

    更新日期:2011-05-01 00:00:00

  • Influence of carbon monoxide on metabolite formation in Methanosarcina acetivorans.

    abstract::Methanogenic archaea conserve energy for growth by reducing some one- and two-carbon compounds to methane and concomitantly generating an ion motive force. Growth of Methanosarcina acetivorans on carbon monoxide (CO) is peculiar as it involves formation of, besides methane, formate, acetate and methylated thiols. It h...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Oelgeschläger E,Rother M

    更新日期:2009-03-01 00:00:00

  • The response regulator Spo0A from Bacillus subtilis is efficiently phosphorylated in Escherichia coli.

    abstract::The response regulator proteins of two-component systems mediate many adaptations of bacteria to their ever-changing environment. Most response regulators are transcription factors that alter the level of transcription of specific sets of genes. Activation of response regulators requires their phosphorylation on a con...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ladds JC,Muchová K,Blaskovic D,Lewis RJ,Brannigan JA,Wilkinson AJ,Barák I

    更新日期:2003-06-27 00:00:00

  • Mucilaginibacter dorajii sp. nov., isolated from the rhizosphere of Platycodon grandiflorum.

    abstract::A Gram-negative, nonmotile and rod-shaped bacterial strain was isolated from the rhizosphere of Platycodon grandiflorum in a study of bacterial diversity, and its taxonomic position was investigated by a genotypic and phenotypic analysis. This isolate, designated as DR-f4, grew at 4-30 degrees C (optimally at 20-25 de...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kim BC,Lee KH,Kim MN,Lee J,Shin KS

    更新日期:2010-08-01 00:00:00

  • Temperature-sensitive recombinant subtilisin protease variants that efficiently degrade molecular biology enzymes.

    abstract::We used error-prone PCR to generate mutations in a subtilisin protease-encoding gene, and screened for recombinants that expressed temperature-sensitive (TS) variants. From the dozens of mutations that we detected in the recombinant genes we found that those mutations that affected aspartate-75 had the most profound e...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Thompson VC,McGuire BE,Frier MS,Legg MSG,Dyer TW,Gudavicius G,Potter S,Nano FE

    更新日期:2020-10-16 00:00:00

  • Oligonucleotide microarray for identification of Bacillus anthracis based on intergenic transcribed spacers in ribosomal DNA.

    abstract::We developed a DNA microarray for identification of Bacillus anthracis and other phylogenetic groupings within the "Bacillus cereus group". Nucleotide sequences of 16S-23S ribosomal DNA internal transcribed spacers containing genes for tRNA(Ile) from 52 B. anthracis strains were found to be identical to sequences from...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Nübel U,Schmidt PM,Reiss E,Bier F,Beyer W,Naumann D

    更新日期:2004-11-15 00:00:00

  • Conformational plasticity of molecular chaperones involved in periplasmic and outer membrane protein folding.

    abstract::How proteins reach their native conformation and location has been a major question of biology during the last 50 years. To counterbalance protein misfolding and the accumulation of aggregation products, a complex network of chaperones and proteases takes care of protein quality control in the cell. Such a chaperone n...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Mas G,Hiller S

    更新日期:2018-07-01 00:00:00

  • Genomic heterogeneity in Chlorobium limicola: chromosomic and plasmidic differences among strains.

    abstract::Chromosome analysis by pulsed-field gel electrophoresis shows a high level of genetic heterogeneity between the two subspecies of the green sulfur bacteria Chlorobium limicola analysed: C. limicola and C. limicola f. s. thiosulfatophilum. Currently, they are differentiated only by the ability to utilize thiosulfate as...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Méndez-Alvarez S,Pavoń V,Esteve I,Guerrero R,Gaju N

    更新日期:1995-12-15 00:00:00

  • Use of an automatic DNA sequencer for S1 mapping: transcriptional analysis of the Streptomyces coelicolor A3(2) dnaK operon.

    abstract::The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The -35 and -10 hexamers correspond to a sigma 70-type promoter. This promoter responds to heat shock and involves an inverted repeat different from t...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Brans A,Loriaux A,Thamm I,Joris B,Dusart J

    更新日期:1997-04-15 00:00:00

  • Analysis of Bordetella pertussis cya operon regulation by use of cya-lac fusions.

    abstract::In Bordetella pertussis virulence-associated genes, including adenylate cyclase toxin (Cya), are coordinately regulated in response to environmental signals by proteins coded by the bvg-locus. We have constructed cya-lac fusions in Escherichia coli and have shown that the cya operon is not expressed in E. coli, neithe...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Goyard S,Ullmann A

    更新日期:1991-01-15 00:00:00

  • Influence of isolate origin and presence of various genes on biofilm formation by Enterococcus faecium.

    abstract::Enterococcus faecium, a major cause of nosocomial infections, is often isolated from conditions where biofilm is considered to be important in the establishment of infections. We investigated biofilm formation among E. faecium isolates from diverse sources and found that the occurrence and amount of biofilm formation ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Almohamad S,Somarajan SR,Singh KV,Nallapareddy SR,Murray BE

    更新日期:2014-04-01 00:00:00

  • Low-moisture food matrices as probiotic carriers.

    abstract::To exert a beneficial effect on the host, adequate doses of probiotics must be administered and maintaining their viability until consumption is thus essential. Dehydrated probiotics exhibit enhanced long-term viability and can be incorporated into low-moisture food matrices, which also possess high stability at refri...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Marcial-Coba MS,Knøchel S,Nielsen DS

    更新日期:2019-01-01 00:00:00

  • Quantitative polymerase chain reaction used for the rapid detection of Carnobacterium species from French soft cheeses.

    abstract::An identification method by PCR, specific to the Carnobacterium genus, was optimised by testing it on 28 bacterial strains. Primers from the literature were tested to differentiate Carnobacterium strains present among various bacterial species. The DNA of Carnobacterium species (C. alterfunditum, C. divergens, C. fund...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cailliez-Grimal C,Miguindou-Mabiala R,Leseine M,Revol-Junelles AM,Millière JB

    更新日期:2005-09-01 00:00:00

  • Evaluation of the catalase promoter for expressing the alkaline xylanase gene (alx) in Aspergillus niger.

    abstract::Aspergillus niger represents a promising host for the expression of recombinant proteins, but only a few expression systems are available for this organism. In this study, the inducible catalase promoter (PcatR) from A. niger was characterized. For this, constructs were developed and checked for the expression of the ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sharma R,Katoch M,Govindappa N,Srivastava PS,Sastry KN,Qazi GN

    更新日期:2012-02-01 00:00:00

  • Utilization of D-ribose through D-xylose transporter.

    abstract::An Escherichia coli mutant defective in high-affinity D-ribose transport is able to utilize the sugar as a sole carbon source, suggesting that other transport systems for D-ribose exist. In order to search for such transporters, transposon mutagenesis was carried out in an rbsB-negative strain containing ribokinase (r...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Song S,Park C

    更新日期:1998-06-15 00:00:00

  • Evidence for xylooligosaccharide utilization in Weissella strains isolated from Indian fermented foods and vegetables.

    abstract::Six strains isolated from fermented food were identified as Weissella species by 16S rDNA sequencing, clustering with the species pair W. confusa/W. cibaria. The strains were analysed for growth on glucose, xylose and xylooligosaccharides (XOS). All strains were xylose positive using the API CHL 50 test. Growth on XOS...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Patel A,Falck P,Shah N,Immerzeel P,Adlercreutz P,Stålbrand H,Prajapati JB,Holst O,Nordberg Karlsson E

    更新日期:2013-09-01 00:00:00

  • Biotransformation of trinitrotoluene by Citrobacter sp. YC4 and evaluation of its cyto-toxicological effects.

    abstract::Trinitrotoluene (TNT) is an explosive chemical generally used for military, civil and industrial purposes. Therefore, TNT residue can be found in soil and ground water as an environmental pollutant. The environmental control of TNT pollution has become a critical issue because of its potential toxicity and carcinogeni...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kao CM,Wei SF,Chen SC,Yao CL,Ma C,Chien CC

    更新日期:2018-01-01 00:00:00

  • Tools for discovery of inhibitors of the 1-deoxy-D-xylulose 5-phosphate (DXP) synthase and DXP reductoisomerase: an approach with enzymes from the pathogenic bacterium Pseudomonas aeruginosa.

    abstract::Two Pseudomonas aeruginosa genes encoding the enzymes 1-deoxy-D-xylulose 5-phosphate (DXP) synthase and DXP reductoisomerase, both involved in the mevalonate-independent biosynthesis of isoprenoids, have been expressed as recombinant enzymes in Escherichia coli. The purified P. aeruginosa DXP reductoisomerase was inhi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Altincicek B,Hintz M,Sanderbrand S,Wiesner J,Beck E,Jomaa H

    更新日期:2000-09-15 00:00:00

  • The Pseudomonas aeruginosa phaG gene product is involved in the synthesis of polyhydroxyalkanoic acid consisting of medium-chain-length constituents from non-related carbon sources.

    abstract::We recently identified the phaG(Pp) gene encoding (R)-3-hydroxydecanoyl-ACP:CoA transacylase in Pseudomonas putida, which directly links the fatty acid de novo biosynthesis and polyhydroxyalkanoate (PHA) biosynthesis. An open reading frame (ORF) of which the deduced amino acid sequence shared about 57% identity with P...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hoffmann N,Steinbüchel A,Rehm BH

    更新日期:2000-03-15 00:00:00

  • Presence of non-suppressive, M2-related dsRNAs molecules in Saccharomyces cerevisiae strains isolated from spontaneous fermentations.

    abstract::Total dsRNA extractions in five killer K2 strains of Saccharomyces cerevisiae isolated from spontaneous fermentations revealed the presence of a novel dsRNA fragment (which we named NS dsRNA) of approximately 1.30 kb, together with L and M2 dsRNAs. NS dsRNA appeared to be encapsidated in the same kind of viral particl...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cansado J,Barros Velázquez J,Sieiro C,Gacto M,Villa TG

    更新日期:1999-12-15 00:00:00

  • Dissimilatory Fe(III) reduction by Clostridium beijerinckii isolated from freshwater sediment using Fe(III) maltol enrichment.

    abstract::A microorganism which reduces Fe(III) during the fermentation of glucose was isolated from freshwater sediment. The Fe(III) was supplied to enrichment cultures as a soluble complex with the bidentate ligand maltol (3-hydroxy-2-methyl-4-pyrone). Advantages that were afforded by the use of Fe(III)(maltol)3 over previous...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Dobbin PS,Carter JP,Garcia-Salamanca San Juan C,von Hobe M,Powell AK,Richardson DJ

    更新日期:1999-07-01 00:00:00