Genetic engineering of Streptococcus gordonii for the simultaneous display of two heterologous proteins at the bacterial surface.

abstract：:Twitching motility is a form of bacterial translocation over solid or semi-solid surfaces mediated by the extension, tethering, and subsequent retraction of type IV pili. These pili are also known to be involved in virulence, biofilm formation, formation of fruiting bodies, horizontal gene transfer, and protein secret...

journal_title：FEMS microbiology letters

authors： Coil DA,Anné J

更新日期：2009-04-01 00:00:00

abstract：:The nucleotide sequence of the gene specifying the ethidium efflux system of Escherichia coli has been determined. The translated open reading frame has identified a membrane-bound polypeptide of 110 amino acids (11,960 Da) which shares 42% identity with a staphylococcal protein specifying resistance to ethidium. ...

journal_title：FEMS microbiology letters

authors： Purewal AS

更新日期：1991-08-01 00:00:00

abstract：:Bacillus anthracis lethal toxin (PALF) stimulated the proliferation of human peripheral blood T-cells in vitro. Activation of T-lymphocytes by PALF required the presence of monocytes and did not result from a collaborative effect between T-cells and B-cells. PALF acted directly on monocytes and independently of T-cell...

journal_title：FEMS microbiology letters

authors： Guidi-Rontani C,Duflot E,Mock M

更新日期：1997-12-15 00:00:00

abstract：:Using natural rubber latex as the sole source of carbon and energy 50 rubber-degrading bacteria were isolated. Out of those 50 isolates, 33 were identified as Streptomyces species and 8 as Micromonospora species. Screening of 1220 bacteria obtained from different culture collections revealed 46 additional rubber-degra...

journal_title：FEMS microbiology letters

authors： Jendrossek D,Tomasi G,Kroppenstedt RM

更新日期：1997-05-15 00:00:00

abstract：:The genes for fructosyltransferase (ftf) and glucan-binding protein (gbp) of Streptococcus mutans strain Ingbritt have been cloned in E. coli using bacteriophage and plasmid vectors. A single ftf gene appears to be responsible for the appearance of several FTF of different electrophoretic mobilities. Despite the fact ...

journal_title：FEMS microbiology letters

authors： Aduse-Opoku J,Gilpin ML,Russell RR

更新日期：1989-06-01 00:00:00

abstract：:In the transition to the post-petroleum economy, there is a growing demand for novel enzymes with high process performances to replace traditional chemistry with a more 'green' approach. To date, microorganisms encompass the richest source of industrial biocatalysts, but the Earth-living microbiota remains largely unt...

journal_title：FEMS microbiology letters

authors： Berini F,Casciello C,Marcone GL,Marinelli F

更新日期：2017-11-15 00:00:00

abstract：:Two conservative gene clusters, the S10 ribosomal protein region and one (of the two) set of rRNA genes, were split in a genome crossover rearrangement event in Mycoplasma gallisepticum. As a result of the rearrangement the major part of the S10 ribosomal protein cluster is located upstream of genes for 23S-5S rRNA (r...

journal_title：FEMS microbiology letters

authors： Skamrov A,Feoktistova E,Goldman M,Beabealashvilli R

更新日期：2002-03-05 00:00:00

abstract：:Among aerobic prokaryotes, many different terminal oxidase complexes have been described. Sequence comparison has revealed that the aa3-type cytochrome c oxidase and the bo3-type quinol oxidase are variations on the same theme: the heme-copper oxidase. A third member of this family has recently been recognized: the cb...

journal_title：FEMS microbiology letters

authors： van der Oost J,de Boer AP,de Gier JW,Zumft WG,Stouthamer AH,van Spanning RJ

更新日期：1994-08-01 00:00:00

abstract：:The Escherichia coli yehZYXW operon encodes a putative osmoprotectant uptake system of the ABC transporter family. yehZ is identical to osmF, an osmotically inducible gene identified previously. Construction and analysis of a yehZ-lacZ transcriptional fusion demonstrated that yehZ is inducible not only by osmolarity, ...

journal_title：FEMS microbiology letters

authors： Checroun C,Gutierrez C

更新日期：2004-07-15 00:00:00

abstract：:The emergence of antibiotic resistance has necessitated new therapeutic approaches for combating persistent bacterial infection. An alternative approach is regulation of bacterial virulence instead of growth suppression, which can readily lead to drug resistance. The virulence of the opportunistic human pathogen Pseud...

journal_title：FEMS microbiology letters

authors： Lee JH,Kim YG,Cho MH,Kim JA,Lee J

更新日期：2012-04-01 00:00:00

abstract：:Calcium has been reported to play an important role in regulating the intestinal electrolyte transport via Ca2+/calmodulin (CaM) and/or protein kinase C (PKC) systems. The role of Ca2+, CaM and PKC in the pathogenesis of Campylobacter jejuni-induced fluid accumulation was studied in vivo in ligated rat ileal loops. Ca...

journal_title：FEMS microbiology letters

authors： Kaur R,Ganguly NK,Kumar L,Walia BN

更新日期：1993-08-01 00:00:00

abstract：:The 2.5-kb erythromycin resistance (EmR) plasmid pPV142 of Staphylococcus simulans 13044 was isolated and characterized. Sequence analysis identified ORF1 and ORF2 encoding a 158-residue replication protein (Rep142) and a 244-residue erythromycin resistance protein (Erm, rRNA adenine N-6-methyltransferase), respective...

journal_title：FEMS microbiology letters

authors： Somkuti GA,Solaiman DK,Steinberg DH

更新日期：1998-08-15 00:00:00

abstract：:We have tested the expression of alkaline phosphatases in a mutant strain of Escherichia coli deficient in dipZ, a gene coding for a protein involved in cytochrome c biogenesis, and the isogenic wild-type strain. The yield of soluble and active human placental alkaline phosphatase was significantly reduced in the muta...

journal_title：FEMS microbiology letters

authors： Beck R,Crooke H,Jarsch M,Cole J,Burtscher H

更新日期：1994-12-01 00:00:00

abstract：:Sphingobacterium sp. RB, a novel bacterial strain isolated from a soil sample, was able to utilize para-nitrophenol (PNP) as sole source of carbon and energy at high concentrations (1.0-5.0 mM). The culture completely degraded 3.0 mM PNP within 36 h with proportionate increase in biomass. With 5.0 mM PNP (700 ppm), 70...

journal_title：FEMS microbiology letters

authors： Samson R,Bodade R,Zinjarde S,Kutty R

更新日期：2019-07-01 00:00:00

abstract：:The filamentous fungi Monascus spp., which have been used in traditional fermented food in Asia for centuries, are well-known producers of a group of bioactive metabolites that are widely used as food additives and nutraceutical supplements worldwide. However, its potential to produce the mycotoxin citrinin poses a th...

journal_title：FEMS microbiology letters

authors： Li L,Shao Y,Li Q,Yang S,Chen F

更新日期：2010-07-01 00:00:00

abstract：:Escherichia coli heat stable enterotoxin (STa) binds to isolated rat intestinal epithelial cells and triggers a cascade reaction including increase of intracellular calcium levels ([Ca(2+)](i)) and membrane bound protein kinase C (PKC) activity. In response to STa, the cytosolic PKC activity falls from 110 to 35 nmol ...

journal_title：FEMS microbiology letters

authors： Ganguly U,Chaudhury AG,Basu A,Sen PC

更新日期：2001-10-16 00:00:00

abstract：:Coagulase-negative staphylococci (CoNS) are common bacterial colonizers of the human skin. They are often involved in nosocomial infections due to biofilm formation in indwelling medical devices. While biofilm formation has been extensively studied in Staphylococcus epidermidis, little is known regarding other CoNS sp...

journal_title：FEMS microbiology letters

authors： Oliveira F,Lima CA,Brás S,França Â,Cerca N

更新日期：2015-10-01 00:00:00

abstract：:Transposon mini-Tn7 vectors insert into the chromosome of several Gram-negative bacteria in a site-specific manner. Here, we showed the application of mini-Tn7 as single copy site-specific integration vector system for Xanthomonas campestris pv. campestris. The transposition of the mini-Tn7 into the bacterial genome w...

journal_title：FEMS microbiology letters

authors： Jittawuttipoka T,Buranajitpakorn S,Fuangthong M,Schweizer HP,Vattanaviboon P,Mongkolsuk S

更新日期：2009-09-01 00:00:00

abstract：:Serum opacity factor (SOF) is produced by group A streptococci belonging to certain M types. SOF cleaves the apolipoprotein component of the high density lipoprotein fraction of serum rendering it insoluble which in turn leads to serum opacity. SfbII protein, a fibronectin binding surface protein cloned from group A s...

journal_title：FEMS microbiology letters

authors： Kreikemeyer B,Martin DR,Chhatwal GS

更新日期：1999-09-15 00:00:00

abstract：:Piscirickettsia salmonis is a novel, aggressive, facultative Gram-negative bacterium that drastically affects salmon production at different latitudes, with particular impact in southern Chile. Initially, P. salmonis was described as a Rickettsia-like, obligate, intracellular Alphaproteobacteria, but it was reclassifi...

journal_title：FEMS microbiology letters

authors： Marshall SH,Henríquez V,Gómez FA,Cárdenas C

更新日期：2011-01-01 00:00:00

abstract：:The glnAntrBC operon of Proteus vulgaris was cloned and heterologously expressed in Escherichia coli. The nucleotide sequence was determined. An open reading frame of 1407 bp was identified as the glnA gene and the deduced amino acid sequence showed 82% identity with the E. coli glutamine synthetase protein. Heterolog...

journal_title：FEMS microbiology letters

authors： Steglitz-Mörsdorf U,Mörsdorf G,Kaltwasser H

更新日期：1993-01-15 00:00:00

abstract：:The phylogenetic position of an acidophilic chemo-organotrophic menaquinone-containing bacterium, Acidobacterium capsulatum, was studied on the basis of 16S rRNA gene sequence information. A. capsulatum showed the highest level of sequence similarity to Heliobacterium chlorum, a member of the Gram-positive group, yet ...

journal_title：FEMS microbiology letters

authors： Hiraishi A,Kishimoto N,Kosako Y,Wakao N,Tano T

更新日期：1995-10-01 00:00:00

abstract：:The outer membrane protein (OMP) profiles of four different strains of Bacteroides fragilis, as determined by Coomassie blue stained polyacrylamide gels, were compared after growth in broth culture and in the mouse peritoneal cavity. There was no induction of the expression of large quantities of novel OMP after growt...

journal_title：FEMS microbiology letters

authors： Patrick S,Lutton DA

更新日期：1990-09-01 00:00:00

abstract：:Triclosan is the active ingredient in a multitude of health care and consumer products with germicidal properties, which have flooded the market in recent years in response to the public's fear of communicable bacteria. Although originally thought to kill bacteria by attacking multiple cellular targets, triclosan was ...

journal_title：FEMS microbiology letters

authors： Schweizer HP

更新日期：2001-08-07 00:00:00

abstract：:The Bacteroides fragilis enterotoxin is an extracellular zinc metalloprotease that has been implicated in diarrheal disease of humans and animals. This toxin causes fluid accumulation in intestinal loops and is cytotoxic for HT-29 cells, an intestinal carcinoma cell line. Here we report the cloning and sequencing of t...

journal_title：FEMS microbiology letters

authors： Kling JJ,Wright RL,Moncrief JS,Wilkins TD

更新日期：1997-01-15 00:00:00

abstract：:We have studied the intraperoxisomal location of catalase in peroxisomes of methanol-grown Hansenula polymorpha by (immuno)cytochemical means. In completely crystalline peroxisomes, in which the crystalline matrix is composed of octameric alcohol oxidase (AO) molecules, most of the catalase protein is located in a nar...

journal_title：FEMS microbiology letters

authors： Keizer I,Roggenkamp R,Harder W,Veenhuis M

更新日期：1992-05-15 00:00:00

abstract：:How proteins reach their native conformation and location has been a major question of biology during the last 50 years. To counterbalance protein misfolding and the accumulation of aggregation products, a complex network of chaperones and proteases takes care of protein quality control in the cell. Such a chaperone n...

journal_title：FEMS microbiology letters

authors： Mas G,Hiller S

更新日期：2018-07-01 00:00:00

abstract：:mlrA is the only microcystin-degrading gene detected in Sphingomonas sp. MJ-PV. The gene has an extremely rare nucleotide sequence and homologous genes have not yet been discovered in the DNA database. We discovered the existence of a gene homologous to mlrA in new microcystin-degrading bacteria, MD-1 and Y2. These st...

journal_title：FEMS microbiology letters

authors： Saito T,Okano K,Park HD,Itayama T,Inamori Y,Neilan BA,Burns BP,Sugiura N

更新日期：2003-12-12 00:00:00

abstract：:Lactic acid bacteria (LAB) have a long history in industrial processes as food starters and biocontrol agents, and also as producers of high-value compounds. Lactic acid, their main product, is among the most requested chemicals because of its multiple applications, including the synthesis of biodegradable plastic pol...

journal_title：FEMS microbiology letters

authors： Tarraran L,Mazzoli R

更新日期：2018-08-01 00:00:00

abstract：:DNA sequence analysis of the nadA gene of Ehrlichia chaffeensis revealed a 942 bp open reading frame with the capacity to encode 314 amino acids. The amino acid sequence of the E. chaffeensis quinolinate synthetase A (NAD/A) has 53.6% identity and 82% similarity to the NAD A of the cyanelle of Cyanophora paradoxa. Por...

journal_title：FEMS microbiology letters

authors： Yu XJ,Walker DH

更新日期：1997-09-01 00:00:00