Abstract:
:The 2.5-kb erythromycin resistance (EmR) plasmid pPV142 of Staphylococcus simulans 13044 was isolated and characterized. Sequence analysis identified ORF1 and ORF2 encoding a 158-residue replication protein (Rep142) and a 244-residue erythromycin resistance protein (Erm, rRNA adenine N-6-methyltransferase), respectively. Structural analysis and Southern hybridization showed that the rep and ermM genes in pPV142 shared homology with the EmR plasmid pPV141 (2.4 kb) of S. chromogenes 3688 and other EmR plasmids known to exist in staphylococci and bacilli. Based on the presence of a 61-bp repeat upstream of the ermM gene, pPV142 is apparently a unique member of the pSN2 family of EmR plasmid able to express erythromycin resistance constitutively.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Somkuti GA,Solaiman DK,Steinberg DHdoi
10.1111/j.1574-6968.1998.tb13158.xsubject
Has Abstractpub_date
1998-08-15 00:00:00pages
281-8issue
2eissn
0378-1097issn
1574-6968pii
S0378-1097(98)00277-8journal_volume
165pub_type
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