Abstract：
:Escherichia coli heat stable enterotoxin (STa) binds to isolated rat intestinal epithelial cells and triggers a cascade reaction including increase of intracellular calcium levels ([Ca(2+)](i)) and membrane bound protein kinase C (PKC) activity. In response to STa, the cytosolic PKC activity falls from 110 to 35 nmol with increase of membrane bound PKC activity from 15 to 78 nmol. Furthermore, the increase of PKC activity induced by STa treatment was always preceded by an increase in [Ca(2+)](i). Cytosolic [Ca(2+)](i) was significantly higher (161 nM) in STa treated cells as compared to untreated cells (51.3 nM). In addition, immunoblot performed on extracts of STa treated rat enterocytes with a monoclonal antibody against PKC alpha showed a prominent band of PKC alpha. Translocation of PKC alpha could be blocked by dantrolene, a drug which inhibits the mobilisation of [Ca(2+)](i) from the intracellular store. Our results, therefore, provide evidence for the role of [Ca(2+)](i) in STa treated cells for the translocation of PKC alpha from cytosol to membrane.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Ganguly U,Chaudhury AG,Basu A,Sen PCdoi
10.1111/j.1574-6968.2001.tb10864.xkeywords:
subject
Has Abstractpub_date
2001-10-16 00:00:00pages
65-9issue
1eissn
0378-1097issn
1574-6968pii
S0378109701003731journal_volume
204pub_type
杂志文章abstract：:As shown by both bioassay and high-performance liquid chromatographic (HPLC) analysis, penicillin G production by Aspergillus nidulans is subject to regulation by the pH of the growth medium. Penicillin titres were highest at alkaline pH and in strains carrying mutations in the regulatory gene pacC which mimics the ef...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/0378-1097(91)90553-m
更新日期：1991-01-15 00:00:00
abstract：:To elucidate the molecular mechanism by which the MexR repressor regulates expression of the MexAB-OprM efflux pump, we investigated MexR and the mexR-mexA intergenic DNA (mexOP) interaction, and transcription of the mexA-lacZ reporter gene containing different lengths of mexOP. Homogeneously purified MexR bound speci...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2001.tb10492.x
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/j.femsle.2004.09.013
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2002.tb11056.x
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journal_title：FEMS microbiology letters
pub_type： 杂志文章,评审
doi：10.1093/femsle/fnx270
更新日期：2018-02-01 00:00:00
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/0378-1097(91)90232-y
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journal_title：FEMS microbiology letters
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doi：10.1111/j.1574-6968.2000.tb09200.x
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abstract：:Biogenic amines have undesirable physiological effects when absorbed at too high a concentration. Several kinds of food and beverages contain biogenic amines. Lactic acid bacteria can decarboxylate amino acids. Since winemaking involves the growth of lactic acid bacteria for malolactic fermentation, biogenic amines ma...
journal_title：FEMS microbiology letters
pub_type： 杂志文章,评审
doi：10.1111/j.1574-6968.2001.tb10643.x
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journal_title：FEMS microbiology letters
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doi：10.1111/1574-6968.12398
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
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更新日期：2001-12-18 00:00:00
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2001.tb10902.x
更新日期：2001-11-13 00:00:00
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/j.femsle.2005.05.020
更新日期：2005-07-01 00:00:00
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/0378-1097(92)90616-v
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1998.tb13177.x
更新日期：1998-09-01 00:00:00
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/j.femsle.2005.06.024
更新日期：2005-08-15 00:00:00
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2007.00673.x
更新日期：2007-05-01 00:00:00
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
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journal_title：FEMS microbiology letters
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journal_title：FEMS microbiology letters
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更新日期：2001-02-20 00:00:00
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
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更新日期：2007-05-01 00:00:00
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journal_title：FEMS microbiology letters
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1996.tb08174.x
更新日期：1996-05-15 00:00:00
abstract：:The fatty acid composition during the transformation of Trypanosoma cruzi epimastigotes into metacyclic trypomastigotes (metacyclogenesis) was analysed by gas-liquid chromatography and mass spectrometry. Significant qualitative and quantitative changes in the fatty acid composition occurred during incubation of epimas...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
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abstract：:A polymerase chain reaction (PCR) amplification of 16S rDNA was developed to identify spirochaetes recovered from cases of intestinal spirochaetosis in humans and pigs; these bacteria belong to a distinct genetic group of spirochaetes, with the proposed name 'Anguillina coli'. The PCR incorporated a universal eubacter...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1995.tb07362.x
更新日期：1995-01-15 00:00:00
abstract：:Crithidia oncopelti, Crithidia deanei and Crithidia desouzai are flagellates of the Trypanosomatidae family that present bacterium-like endosymbionts in their cytoplasm. Gelatin-SDS-PAGE analysis was used to characterize cell-associated and extracellular proteinases in these organisms. Our survey indicates that the pr...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2001.tb10782.x
更新日期：2001-08-07 00:00:00
abstract：:Bacillus subtilis strains BS49 and BS34A, both derived from a common ancestor, carry one or more copies of Tn916, an extremely common mobile genetic element capable of transfer to and from a broad range of microorganisms. Here, we report the complete genome sequence of BS49 and the draft genome sequence of BS34A, whic...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1093/femsle/fnu050
更新日期：2015-01-01 00:00:00
abstract：:Acyl carrier protein (ACP) of Escherichia coli is a small acidic protein which functions as carrier of growing acyl chains during their biosynthesis and as donor of acyl chains during transfer to target molecules. This unique ACP of E. coli is expressed constitutively. In more complex bacteria, multiple ACPs are prese...
journal_title：FEMS microbiology letters
pub_type： 杂志文章,评审
doi：10.1111/j.1574-6968.2002.tb11075.x
更新日期：2002-03-05 00:00:00
abstract：:The genus Xanthomonas is composed of several species that cause severe crop losses around the world. In Latin America, one of the most relevant species is Xanthomonas campestris pv. campestris, which is responsible for black rot in cruciferous plants. This pathogen causes yield losses in several cultures, including ca...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2008.01090.x
更新日期：2008-04-01 00:00:00
