Nucleotide sequence of the ethidium efflux gene from Escherichia coli.

abstract：:To investigate the auto-ecology of a strain of Gigaspora margarita in a commercial inoculum, we found a pair of PCR primers amplifying a sequence of 235 bp diagnostic of the isolate. We designed an oligonucleotide probe based on the DNA sequence. The combination of PCR and the probing successfully detected the diagnos...

journal_title：FEMS microbiology letters

authors： Yokoyama K,Tateishi T,Marumoto T,Saito M

更新日期：2002-07-02 00:00:00

abstract：:This study provides a novel qRT-PCR protocol for specific detection and proof of viability of Phytophthora in environmental samples based on differential accumulation of cox II transcripts. Chemical and physical treatments were tested for their ability to induce in vitro the accumulation of cytochrome oxidase genes en...

journal_title：FEMS microbiology letters

authors： Vannini A,Tomassini A,Bruni N,Vettraino AM

更新日期：2014-04-01 00:00:00

abstract：:The primary structure of a bacteriocin produced by Enterococcus hirae DCH5 was determined by combined amino acid and DNA sequencing. Nucleotide analysis of a 2838-bp DNA fragment of E. hirae DCH5 revealed five putative ORFs. The first orf (hirJM79) encodes a 74-amino-acid peptide containing an N-terminal signal peptid...

journal_title：FEMS microbiology letters

authors： Sánchez J,Diep DB,Herranz C,Nes IF,Cintas LM,Hernández PE

更新日期：2007-05-01 00:00:00

abstract：:Nucleoside diphosphate kinase (NDK) is a housekeeping enzyme localized in cellular organelles and distributed in various organs in prokaryotes and eukaryotes. In Neurospora crassa, NDK-1 is suggested to control catalases in response to heat, oxidative stress and light. In this study, we identified the presence of NDK-...

journal_title：FEMS microbiology letters

authors： Yoshida Y,Hasunuma K

更新日期：2006-08-01 00:00:00

abstract：:The phosphatidylcholine (PC) content of Aspergillus nidulans choC was varied by growing the auxotroph in medium containing various concentrations of choline chloride. Direct linear correlations were observed between PC content and in vivo chitin synthase activity, between in vivo chitin synthase activity and mean hyph...

journal_title：FEMS microbiology letters

authors： Binks PR,Robson GD,Goosey MW,Trinci AP

更新日期：1991-10-01 00:00:00

abstract：:Proteus mirabilis strain MAG1, a clinical isolate that is resistant to broad-spectrum penicillins and co-amoxiclav, produces inhibitor-resistant TEM (IRT)-21, a novel mutant of TEM beta-lactamase. This enzyme has a pI of 5.2 and is derived from the bla(TEM-1a) gene ancestor. It contains two major amino acid substituti...

journal_title：FEMS microbiology letters

authors： Mammeri H,Gilly L,Laurans G,Vedel G,Eb F,Paul G

更新日期：2001-12-18 00:00:00

abstract：:Phylogenetically diverse rhizobial strains endemic to Tunisia were isolated from symbiotic nodules of Lotus creticus, growing in different arid extremophile geographical regions of Tunisia, and speciated using multiloci-phylogenetic analysis as Neorhizobium huautlense (LCK33, LCK35, LCO42 and LCO49), Ensifer numidicus...

journal_title：FEMS microbiology letters

authors： Rejili M,BenAbderrahim MA,Mars M,Sherrier JD

更新日期：2020-06-01 00:00:00

abstract：:We developed an improved HEp-2 cell assay method for the detection of Bacillus cereus toxin, which affects the proliferation of HEp-2 cells. The cytostatic toxin was stable upon exposure to heat, pH 2, pH 11 and trypsin, which suggests it is an emetic. Using the HEp-2 cell assay, we examined the distribution and conta...

journal_title：FEMS microbiology letters

authors： Mikami T,Horikawa T,Murakami T,Matsumoto T,Yamakawa A,Murayama S,Katagiri S,Shinagawa K,Suzuki M

更新日期：1994-06-01 00:00:00

abstract：:Escherichia coli heat stable enterotoxin (STa) binds to isolated rat intestinal epithelial cells and triggers a cascade reaction including increase of intracellular calcium levels ([Ca(2+)](i)) and membrane bound protein kinase C (PKC) activity. In response to STa, the cytosolic PKC activity falls from 110 to 35 nmol ...

journal_title：FEMS microbiology letters

authors： Ganguly U,Chaudhury AG,Basu A,Sen PC

更新日期：2001-10-16 00:00:00

abstract：:Comparisons of growth and surface colonisation of Enterococcus faecium L-forms and their cell-walled forms were undertaken to produce information about their ability to form sessile cells. The growth of L-forms in liquid culture was slower than that of the parent. This was reflected in their longer lag phase and slowe...

journal_title：FEMS microbiology letters

authors： Jass J,Phillips LE,Allan EJ,Costerton JW,Lappin-Scott HM

更新日期：1994-01-15 00:00:00

abstract：:Pseudomonas aeruginosa PA96 is a clinical isolate from Guangzhou, China, that is multiresistant to antibiotics. We previously described the 500-kb IncP-2 plasmid, pOZ176 that encodes many resistance genes including the IMP-9 carbapenemase. Whole-genome sequencing of PA96 enabled characterization of its genomic islands...

journal_title：FEMS microbiology letters

authors： Déraspe M,Alexander DC,Xiong J,Ma JH,Low DE,Jamieson FB,Roy PH

更新日期：2014-07-01 00:00:00

abstract：:The formation of dormant endospores is a complex morphological process that permits long-term survival in inhospitable environments for many Gram-positive bacteria. Sporulation for the anaerobic gastrointestinal pathogen Clostridium difficile is necessary for survival outside of the gastrointestinal tract of its host....

journal_title：FEMS microbiology letters

authors： Edwards AN,McBride SM

更新日期：2014-09-01 00:00:00

abstract：:Staphylococcus aureus produces a number of virulence factors. The major virulence factors exhibited by S aureus include various antigens, enzymes, cytotoxins and exotoxins (e.g. hemolysins, enterotoxins and toxic shock syndrome toxin). In this report, we show the influence of punicalagin on the secretion of exoprotein...

journal_title：FEMS microbiology letters

authors： Mun SH,Kong R,Seo YS,Zhou T,Kang OH,Shin DW,Kwon DY

更新日期：2016-11-01 00:00:00

abstract：:Infections caused by Streptococcus suis, a major swine pathogen, include meningitis, arthritis, pneumonia and septicaemia. In this study, we investigated interactions that may occur between human brain microvascular endothelial cells (HBMEC), the main constituent of the blood-brain barrier, and S. suis. We show that S...

journal_title：FEMS microbiology letters

authors： Jobin MC,Fortin J,Willson PJ,Gottschalk M,Grenier D

更新日期：2005-11-01 00:00:00

abstract：:Magnetotactic bacteria are microorganisms that respond to magnetic fields. We studied the surface ultrastructure of uncultured magnetotactic cocci collected from a marine environment by transmission electron microscopy using freeze-fracture and freeze-etching. All bacteria revealed a Gram-negative cell wall. Many bact...

journal_title：FEMS microbiology letters

authors： Freitas F,Keim CN,Kachar B,Farina M,Lins U

更新日期：2003-02-14 00:00:00

abstract：:The outer membrane protein (OMP) profiles of four different strains of Bacteroides fragilis, as determined by Coomassie blue stained polyacrylamide gels, were compared after growth in broth culture and in the mouse peritoneal cavity. There was no induction of the expression of large quantities of novel OMP after growt...

journal_title：FEMS microbiology letters

authors： Patrick S,Lutton DA

更新日期：1990-09-01 00:00:00

abstract：:The relationship between sugar availability and RTX (repeats in toxin) cytotoxin (leukotoxin) production in the periodontopathic bacterium, Actinobacillus actinomycetemcomitans, was investigated using a chemostat. A actinomycetemcomitans 301-b produced significant amounts of leukotoxin in anaerobic fructose-limited ch...

journal_title：FEMS microbiology letters

authors： Mizoguchi K,Ohta H,Miyagi A,Kurihara H,Takashiba S,Kato K,Murayama Y,Fukui K

更新日期：1997-01-01 00:00:00

abstract：:The detection of Trypanosoma cruzi kinetoplast DNA by polymerase chain reaction (PCR) amplification is a potentially powerful tool for the parasitological diagnosis of Chagas' disease. We have applied this technique in a field situation in Bolivia, where 45 children from a primary school were subjected to serological ...

journal_title：FEMS microbiology letters

authors： Wincker P,Bosseno MF,Britto C,Yaksic N,Cardoso MA,Morel CM,Brenière SF

更新日期：1994-12-15 00:00:00

abstract：:Two different nucleic acid precursor utilization patterns were obtained for five avian isolates of Chlamydia psittaci. Three of the isolates behaved in a manner similar to that previously described, showing total dependency on the host cell for ribonucleoside triphosphates and being unable to utilize medium-supplied t...

journal_title：FEMS microbiology letters

authors： McClarty G,Fan H,Andersen AA

更新日期：1993-04-15 00:00:00

abstract：:A composite transposon is a mobile genetic element consisting of two insertion sequences (ISs) flanking a segment of cargo DNA often containing antibiotic resistance (AR) genes. Composite transposons can move as a discreet unit. There have been recently several reports on a novel mechanism of movement of an IS26-based...

journal_title：FEMS microbiology letters

authors： Tansirichaiya S,Mullany P,Roberts AP

更新日期：2016-09-01 00:00:00

abstract：:Glutamate uptake in the Gram-positive Corynebacterium glutamicum is mediated via a binding protein-dependent transport system, which is encoded by the gluABCD gene cluster. Cloning of these genes in an expression vector and subsequent transformation of the resulting plasmid allows different strains of the Gram-negativ...

journal_title：FEMS microbiology letters

authors： Burkovski A,Krämer R

更新日期：1995-04-01 00:00:00

abstract：:Nine clinical isolates of Enterobacteriaceae (six Escherichia coli and three Proteus mirabilis) isolated in three Parisian hospitals between 1989 and 2000 showed a particular extended-spectrum cephalosporin-resistance profile characterized by resistance to cefotaxime and aztreonam but not to ceftazidime. CTX-M-1, CTX-...

journal_title：FEMS microbiology letters

authors： Saladin M,Cao VT,Lambert T,Donay JL,Herrmann JL,Ould-Hocine Z,Verdet C,Delisle F,Philippon A,Arlet G

更新日期：2002-04-09 00:00:00

abstract：:The major heat shock proteins from Thiobacillus ferrooxidans were identified as DnaK and GroEL equivalents by Western blotting and analysis of the N-terminal amino acid sequence of spots isolated from dried 2-D polyacrylamide electrophoresis gels. The T. ferrooxidans chaperonins showed 70% and 80% identity with the Es...

journal_title：FEMS microbiology letters

authors： Varela P,Jerez CA

更新日期：1992-11-01 00:00:00

abstract：:A compound with siderophore activity was purified by successive column and thin layer chromatographic procedures from Dowex 1 x 8 extracts of culture supernatants of Vibrio parahaemolyticus AQ 3354. The strain synthesized the compound in culture media containing less than 2 microM added FeCl3. Hydrolysis of the compou...

journal_title：FEMS microbiology letters

authors： Yamamoto S,Fujita Y,Okujo N,Minami C,Matsuura S,Shinoda S

更新日期：1992-07-01 00:00:00

abstract：:In sausage, Staphylococcus carnosus increases the levels of methyl ketones which could arise from incomplete beta-oxidation of fatty acids followed by a decarboxylation. The objective of this work was to characterize the beta-decarboxylase activities in cell-free extract. By using different substrates, at least two ki...

journal_title：FEMS microbiology letters

authors： Fadda S,Leroy-Sétrin S,Talon R

更新日期：2003-11-07 00:00:00

abstract：:A broad distribution of the lactococcal IS elements ISS1 [1] and IS904 [2] in several lactococcal plasmids and chromosomal DNA was observed. Hybridization of the ISS1 and IS904 oligonucleotide gene probes with DNA of lactococcal phages showed that none of these tested bacteriophages contained one of the IS elements. O...

journal_title：FEMS microbiology letters

authors： Schäfer A,Jahns A,Geis A,Teuber M

更新日期：1991-05-15 00:00:00

abstract：:Pseudomonas spp., in particular Pseudomonas fragi, are dominant in aerobically stored chilled meats. This work isolated P. fragi isolates from spoiled chicken and investigated the effect of modified atmosphere packaging (MAP, CO2/N2 30%/70%) on physicochemical properties of P. fragi isolates compared to the correspond...

journal_title：FEMS microbiology letters

authors： Wang GY,Li M,Ma F,Wang HH,Xu XL,Zhou GH

更新日期：2017-06-15 00:00:00

abstract：:Contrary to its effect on rich medium, D-cycloserine showed no bactericidal effect on Zymomonas mobilis cells cultured on mineral medium. Addition of a mixture of glycine and glutamic acid to the mineral medium restored its bactericidal action. However, mutant enrichments run in these conditions were biased, with most...

journal_title：FEMS microbiology letters

authors： Pencreac'h G,Aït-Abdelkader N,Joset F,Baratti JC

更新日期：1996-09-01 00:00:00

abstract：:Burkholderia mallei, the etiologic agent of the disease known as glanders, is primarily a disease affecting horses and is transmitted to humans by direct contact with infected animals. The use of B. mallei as a biological weapon has been reported and currently, there is no vaccine available for either humans or animal...

journal_title：FEMS microbiology letters

authors： Whitlock GC,Estes DM,Torres AG

更新日期：2007-12-01 00:00:00

abstract：:The genes encoding transferrin-binding proteins (TBPs) 1 and 2 of Neisseria meningitidis and N. gonorrhoeae were used as model loci in a novel method of cloning (twin N-terminal polymerase chain reaction; TNT-PCR) involving amplification between the 5' ends of two genes. Primers were based on N-terminal amino-acid seq...

journal_title：FEMS microbiology letters

authors： Wilton J,Ala'Aldeen D,Palmer HM,Borriello SP

更新日期：1993-02-15 00:00:00