Abstract:
:Two different nucleic acid precursor utilization patterns were obtained for five avian isolates of Chlamydia psittaci. Three of the isolates behaved in a manner similar to that previously described, showing total dependency on the host cell for ribonucleoside triphosphates and being unable to utilize medium-supplied thymidine. In contrast, the other two isolates were incapable of taking pyrimidine ribonucleotides from the host cell and they could efficiently utilize medium-supplied thymidine. These unusual isolates were resistant to 5-fluorouridine while the other three isolates were sensitive. Of the five isolates only 6BC was sensitive to sulfonamides. The five isolates were divided into two groups by comparing the AluI restriction endonuclease patterns obtained following digestion of the major outer membrane protein (OMP1) gene, amplified by the polymerase chain reaction. The OMP1 genotyping results were confirmed by serotyping.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
McClarty G,Fan H,Andersen AAdoi
10.1111/j.1574-6968.1993.tb06123.xsubject
Has Abstractpub_date
1993-04-15 00:00:00pages
325-31issue
3eissn
0378-1097issn
1574-6968journal_volume
108pub_type
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