Abstract:
:Pseudomonas aeruginosa PA96 is a clinical isolate from Guangzhou, China, that is multiresistant to antibiotics. We previously described the 500-kb IncP-2 plasmid, pOZ176 that encodes many resistance genes including the IMP-9 carbapenemase. Whole-genome sequencing of PA96 enabled characterization of its genomic islands, virulence factors, and chromosomal resistance genes. We filled gaps using PCR and used optical mapping to confirm the correct contig order. We automatically annotated the core genome and manually annotated the genomic islands. The genome is 6 444 091 bp and encodes 5853 ORFs. From the whole-genome sequence, we constructed a physical map and constructed a phylogenetic tree for comparison with sequenced P. aeruginosa strains. Analysis of known core genome virulence factors and resistance genes revealed few differences with other strains, but the major virulence island is closer to that of DK2 than to PA14. PA96 most closely resembles the environmental strain M18, and notably shares a common serotype, pyoverdin type, flagellar operon, type IV pilin, and several genomic islands with M18.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Déraspe M,Alexander DC,Xiong J,Ma JH,Low DE,Jamieson FB,Roy PHdoi
10.1111/1574-6968.12435subject
Has Abstractpub_date
2014-07-01 00:00:00pages
212-6issue
2eissn
0378-1097issn
1574-6968journal_volume
356pub_type
信件abstract::Incubation of Mycobacterium tuberculosis and M smegmatis cells with the sugar components of their surface-exposed glycans demonstrated that D-arabinose but not alpha-D-glucose or D-mannose, led to the dispersion of the large clumps formed by the bacilli in stationary liquid cultures. These results confirm the presence...
journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:1999-08-15 00:00:00
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journal_title:FEMS microbiology letters
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更新日期:1990-01-01 00:00:00
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journal_title:FEMS microbiology letters
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更新日期:2016-05-01 00:00:00