Abstract:
:We have observed significant cell lysis upon temperature up-shift of recombinant Escherichia coli cultures harboring CI857-repressed lambda-based expression vectors. This event, that becomes evident about 30-40 min after the heat shock, takes place when using the lambda promoter system in Ind- lysogenic strains, but not in others commonly employed for recombinant gene expression. These results strongly suggest that the thermosensitive CI857 repressor, encoded by the expression vector, competes with CI Ind- molecules for binding to the prophage operator region, allowing for expression of lytic genes from the integrated Ind- viral genome upon temperature up-shift. Transcription of viral lytic genes does not include unspecific expression of a reporter sulA::lacZ gene fusion carried in the prophage genome. These results prompt, however, to carefully evaluate the limitations of expression systems based on pL/pR-CI857 in bacterial strains modified through lambda Ind- gene transfer vehicles.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Hoffmann F,Arís A,Carbonell X,Rohde M,Corchero JL,Rinas U,Villaverde Adoi
10.1111/j.1574-6968.1999.tb13750.xkeywords:
subject
Has Abstractpub_date
1999-08-15 00:00:00pages
327-34issue
2eissn
0378-1097issn
1574-6968pii
S0378-1097(99)00334-1journal_volume
177pub_type
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journal_title:FEMS microbiology letters
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