Abstract:
:Five transposon mutants of Francisella novicida were isolated that are compromised in their ability to grow in mouse macrophages in vitro. Sequence analysis of the DNA flanking the transposon insertions identified the genes that were interrupted in these mutants. One of the inactivated loci corresponds to the Francisella tularensis gene that encodes a 23-kDa protein that is the most prominently induced protein following macrophage infection. Another insertion was localised to approximately 2 kb upstream of the gene encoding the 23-kDa protein. By analysis of the incomplete Francisella genome sequence it was surmised that these two insertions disrupt different portions of a putative operon that encodes four proteins, none of which have discernible functions. Three other interrupted loci associated with poor intramacrophage growth showed similarity at the deduced amino acid level to alanine racemase, the ClpB heat-shock protease, and the purine biosynthetic enzyme, glutamine phosphoribosylpyrophosphate amidotransferases.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Gray CG,Cowley SC,Cheung KK,Nano FEdoi
10.1111/j.1574-6968.2002.tb11369.xkeywords:
subject
Has Abstractpub_date
2002-09-24 00:00:00pages
53-6issue
1eissn
0378-1097issn
1574-6968pii
S0378109702009114journal_volume
215pub_type
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