Mice immunization with live lactococci displaying a surface anchored HPV-16 E7 oncoprotein.

abstract：:Tachyzoites of Toxoplasma gondii multiply within the parasitophorous vacuole (PV) until the lysis of the host cell. This study was undertaken to evaluate the effect of hydroxyurea (a specific drug that arrests cell division at G1/S phase) on the multiplication of T. gondii tachyzoites in infected Vero cells. Infected ...

journal_title：FEMS microbiology letters

authors： de Melo EJ,Mayerhoffer RO,de Souza W

更新日期：2000-04-01 00:00:00

abstract：:The classical monomeric autotransporters are ubiquitously used by Gram-negative bacteria to export virulence and colonization factors to their cell surface or into their surroundings. They are expressed as monomeric proteins that pass the inner and outer membrane in two consecutive steps facilitated by the Sec translo...

journal_title：FEMS microbiology letters

authors： van Ulsen P,Zinner KM,Jong WSP,Luirink J

更新日期：2018-09-01 00:00:00

abstract：:The nitrogen-fixing cyanobacterium Nostoc sp. ATCC 29132 was shown to contain two sequence-specific endonucleases. Nsp(29132) I was an isoschizomer of AsuII, and Nsp(29132) II was an isoschizomer of BamHI. Nsp(29132) II was shown to generate ends that could be ligated to those generated by BamHI. ...

journal_title：FEMS microbiology letters

authors： Muro-Pastor AM,Herrero A,Flores E

更新日期：1991-01-01 00:00:00

abstract：:The xylanase gene of Streptomyces flavogriseus was cloned in pUC8 plasmid and expressed in Escherichia coli lysogenic for lambda cI857. lambda-Induced lysis of E. coli at 42 degrees C allowed efficient release of cloned enzyme activity in extracellular environment. The xylanase gene was located in the 0.8-kb HindIII f...

journal_title：FEMS microbiology letters

authors： Srivastava R,Ali SS,Srivastava BS

更新日期：1991-03-01 00:00:00

abstract：:The pseudotrisaccharide allosamidin, a potent inhibitor of chitinases, retarded the fragmentation of hyphae but did not affect the fungal growth and cephalosporin-C production in Acremonium chrysogenum. In vitro inhibition of A. chrysogenum cell-bound chitinase(s) by allosamidin revealed that about 47% of the soluble ...

journal_title：FEMS microbiology letters

authors： Sándor E,Pusztahelyi T,Karaffa L,Karányi Z,Pócsi I,Biró S,Szentirmai A,Pócsi I

更新日期：1998-07-15 00:00:00

abstract：:Bacillus subtilis strains BS49 and BS34A, both derived from a common ancestor, carry one or more copies of Tn916, an extremely common mobile genetic element capable of transfer to and from a broad range of microorganisms. Here, we report the complete genome sequence of BS49 and the draft genome sequence of BS34A, whic...

journal_title：FEMS microbiology letters

authors： Browne HP,Anvar SY,Frank J,Lawley TD,Roberts AP,Smits WK

更新日期：2015-01-01 00:00:00

abstract：:A hybridized DNA fragment was cloned as a 7.6-kb fragment from Streptomyces lavendulae KCCS0263 using a 1.9-kb SacI-XbaI DNA fragment from S. cellulosae as a probe. The latter fragment encoded a beta-lactamase which can bind blue dextran. The hybridized region was reduced to a 2.8-kb KpnI-BclI fragment and the nucleot...

journal_title：FEMS microbiology letters

authors： Sendouda A,Urabe H,Ogawara H

更新日期：1993-09-15 00:00:00

abstract：:The aims of our research were to investigate the gene expression of the multidrug efflux transporter, CDR1 and the major drug facilitator superfamily transporter, MDR1 gene in azole drug-resistant Candida albicans and Candida glabrata clinical isolates recovered from vaginitis patients; and to identify hotspot mutatio...

journal_title：FEMS microbiology letters

authors： Looi CY,D' Silva EC,Seow HF,Rosli R,Ng KP,Chong PP

更新日期：2005-08-15 00:00:00

abstract：:Effects of the two-component sensor-regulator pairs DctBD and NtrBC upon the expression of a dctA::phoA fusion from Rhizobium meliloti were determined under excess and limiting nitrogen concentrations in Escherichia coli. Results indicated that NtrBC affected transcription from the dctA promoter on a number of regulat...

journal_title：FEMS microbiology letters

authors： Allaway D,Boesten B,O'Gara F

更新日期：1995-05-15 00:00:00

abstract：:In microbial corrinoid-dependent methyltransferase systems, adventitious Co(I)-corrinoid oxidation halts catalysis and necessitates repair by ATP-dependent reductive activases. RamA, an activase with a C-terminal ferredoxin domain with two [4Fe-4S] clusters from methanogenic archaea, has been far less studied than the...

journal_title：FEMS microbiology letters

authors： Huening KA,Jiang R,Krzycki JA

更新日期：2020-09-01 00:00:00

abstract：:Lactic acid bacteria were screened for methional production from 4-methylthio-2-ketobutanoate. Only Lactococcus lactis IFPL730 produced high amounts of methional. It was demonstrated that production of this compound was an exclusively enzymatic reaction. The present work describes for the first time that L. lactis can...

journal_title：FEMS microbiology letters

authors： Amárita F,Fernández-Esplá D,Requena T,Pelaez C

更新日期：2001-10-16 00:00:00

abstract：:The polysaccharides formed on hot alkaline extraction of the ascomycetous lichen Teloschistes flavicans were fractionated to give two glucans, which were characterised by methylation analysis and 1D and 2D NMR spectroscopy. One was a branched beta-glucan containing (1-->3) and (1-->6) linkages, a structure which is mo...

journal_title：FEMS microbiology letters

authors： Reis RA,Tischer CA,Gorin PA,Iacomini M

更新日期：2002-04-23 00:00:00

abstract：:Pyruvate, the key regulator in connection of a variety of metabolic pathways, influences transcription of the Escherichia coli genome through controlling the activity of two pyruvate-sensing two-component systems (TCSs), BtsSR and PyrSR. Previously, we identified the whole set of regulatory targets of PyrSR with low-a...

journal_title：FEMS microbiology letters

authors： Ogasawara H,Ishizuka T,Yamaji K,Kato Y,Shimada T,Ishihama A

更新日期：2019-12-01 00:00:00

abstract：:Pasteurella multocida, a Gram-negative nonmotile coccobacillus, is the causative agent of fowl cholera in poultry, hemorrhagic septicemia in cattle, atropic rhinitis in swine, and snuffles in rabbits. The differentially expressed gene profile of P. multocida in infected rabbit livers was identified and compared with t...

journal_title：FEMS microbiology letters

authors： Guo D,Lu Y,Zhang A,Liu J,Yuan D,Jiang Q,Lin H,Si C,Qu L

更新日期：2012-06-01 00:00:00

abstract：:An inducible mycelial beta-glucosidase from Scytalidum thermophilum was characterized. The enzyme exhibited a pI of 6.5, a carbohydrate content of 15%, and an apparent molecular mass of about 40 kDa. Optima of temperature and pH were 60 degrees C and 6.5, respectively. The enzyme was stable up to 1 h at 50 degrees C a...

journal_title：FEMS microbiology letters

authors： Zanoelo FF,Polizeli Mde L,Terenzi HF,Jorge JA

更新日期：2004-11-15 00:00:00

abstract：:Massive Open Online Courses (MOOCs) dominated discussions of online learning and higher education in the news media and in universities between 2012 and 2015. However, fashions pass, needs change and technology evolves. This Commentary looks back, pauses on the present, and then looks forward. Whilst MOOCs are a signi...

journal_title：FEMS microbiology letters

authors： Daniel J

更新日期：2016-04-01 00:00:00

abstract：:DNA polymerase from the archaeon Pyrococcus abyssi strain Orsay was expressed in Escherichia coli. The recombinant DNA polymerase (Pab) was purified to homogeneity by heat treatment followed by 5 steps of chromatography and characterized for PCR applications. Buffer optimization experiments indicated that Pab PCR perf...

journal_title：FEMS microbiology letters

authors： Dietrich J,Schmitt P,Zieger M,Preve B,Rolland JL,Chaabihi H,Gueguen Y

更新日期：2002-11-19 00:00:00

abstract：:The 181 251 bp accessory plasmid pSmeSM11b of Sinorhizobium meliloti strain SM11, belonging to a dominant indigenous S. meliloti subpopulation identified during a long-term field release experiment, was sequenced. This plasmid has 166 coding sequences (CDSs), 42% of which encode proteins with homology to proteins of k...

journal_title：FEMS microbiology letters

authors： Stiens M,Schneiker S,Pühler A,Schlüter A

更新日期：2007-06-01 00:00:00

abstract：:A two-step enzymatic synthesis process of 4-hydroxyisoleucine is suggested. In the first step, the aldol condensation of acetaldehyde and alpha-ketobutyrate catalyzed by specific aldolase results in the formation of 4-hydroxy-3-methyl-2-keto-pentanoate (HMKP). In the second step, amination of HMKP by the branched-chai...

journal_title：FEMS microbiology letters

authors： Smirnov SV,Samsonova NN,Novikova AE,Matrosov NG,Rushkevich NY,Kodera T,Ogawa J,Yamanaka H,Shimizu S

更新日期：2007-08-01 00:00:00

abstract：:The applicability of different PCR-based techniques, random amplified polymorphic DNA, cleaved amplified polymorphic sequence and SSRs (simple sequence repeats) to genetically identify, typify and discriminate among Saccharomyces cerevisiae strains, was investigated. The analysis and comparison of the genetic profiles...

journal_title：FEMS microbiology letters

authors： Pérez MA,Gallego FJ,Hidalgo P

更新日期：2001-12-18 00:00:00

abstract：:Three heterocytous cyanobacterial strains were isolated from different habitats of Central India, and initial morphological studies indicated them to be members of the genus Nostoc or other closely related genera. Subsequent studies using morphological, ecological, molecular and phylogenetic methods indicated the thre...

journal_title：FEMS microbiology letters

authors： Saraf A,Dawda HG,Suradkar A,Behere I,Kotulkar M,Shaikh ZM,Kumat A,Batule P,Mishra D,Singh P

更新日期：2018-12-01 00:00:00

abstract：:A typing method for Clostridium difficile based on restriction fragment length polymorphisms (RFLP) is described. The technique utilizes commercially available Escherichia coli ribosomal ribonucleic acid (rRNA) as probe material. Probe labelling, hybridization and detection was performed using the Enhanced Chemilumine...

journal_title：FEMS microbiology letters

authors： Bowman RA,O'Neill GL,Riley TV

更新日期：1991-04-15 00:00:00

abstract：:The molecular genetics of indole-3-acetic (IAA) synthesis and regulation in Azospirillum brasilense was investigated in this study. Tn5 mutagenesis was performed and five mutants with decreased IAA production were isolated. Five Tn5-inserted genes from these mutants were cloned and sequenced. Four genes were reported ...

journal_title：FEMS microbiology letters

authors： Xie B,Xu K,Zhao HX,Chen SF

更新日期：2005-07-01 00:00:00

abstract：:Cell wall LPS of Escherichia coli are organized as particles which are visible in the electron microscope, after treatment of the wall with alkali. We now describe alkali treated walls of three E. coli strains with differences in susceptibility to the T4 phage infection. Strain CR63, a usual host for the T4 phage, sho...

journal_title：FEMS microbiology letters

authors： Zorzopulos J,de Long S,Chapman V,Kozloff LM

更新日期：1989-10-01 00:00:00

abstract：:In this report we describe a Lactobacillus fermentum 3872 plasmid (pLF3872) not previously found in any other strain of this species. The analysis of the complete sequence of this plasmid revealed the presence of a gene encoding a large collagen-binding protein (CBP), as well as the genes responsible for plasmid maint...

journal_title：FEMS microbiology letters

authors： Lehri B,Seddon AM,Karlyshev AV

更新日期：2015-06-01 00:00:00

abstract：:Changes in composition of the principal low molecular mass thiols of Leishmania donovani were monitored during the transformation of promastigotes, first to stationary phase metacyclic forms and then to amastigotes. No consistent variation in the thiol composition of the parasite which could account for the known incr...

journal_title：FEMS microbiology letters

authors： Weldrick DP,Chodacka B,Vogt R,Steenkamp DJ

更新日期：1999-04-01 00:00:00

abstract：:Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot disease in cruciferous plants. The synthesis of known virulence factors in this organism, such as extracellular enzymes and biofilm, is strictly regulated in response to environmental stimuli. Two-component signal transduction systems sense e...

journal_title：FEMS microbiology letters

authors： Tao J,He C

更新日期：2010-03-01 00:00:00

abstract：:Helicobacter pylori possesses a gene (HP0326/JHP309) homologous to neuA of other bacteria, encoding a cytidyl monophosphate-N-acetylneuraminic acid synthetase-homologous enzyme in its N-terminal portion. We analysed the function of this gene, which is controlled by a flagellar class 2 sigma(54) promoter, in flagellar ...

journal_title：FEMS microbiology letters

authors： Josenhans C,Vossebein L,Friedrich S,Suerbaum S

更新日期：2002-05-07 00:00:00

abstract：:IS1(SB) and its two variants were identified as the major and minor IS1 elements in Shigella boydii. The nucleotide sequences of IS1(SB), IS1(O157:H7) from Escherichia coli O157:H7 and IS1F from E. coli K12 suggest that these IS1 elements had been horizontally transferred among S. boydii and E. coli O157:H7 and K12. T...

journal_title：FEMS microbiology letters

authors： Hsu WB,Chen JH

更新日期：2003-05-28 00:00:00

abstract：:We present a method to directly characterize the yeast diversity present in wine fermentations by employing denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 26S ribosomal RNA (rRNA) genes. PCR-DGGE of a portion of the 26S rRNA gene was shown to distinguish most yeast genera a...

journal_title：FEMS microbiology letters

authors： Cocolin L,Bisson LF,Mills DA

更新日期：2000-08-01 00:00:00