Sequence analysis of the 181-kb accessory plasmid pSmeSM11b, isolated from a dominant Sinorhizobium meliloti strain identified during a long-term field release experiment.

abstract：:Both ThyA and ThyX proteins catalyze the transfer of the methyl group from methylenetetrahydrofolate (CH(2) H(4) -folate) to dUMP, forming dTMP. To estimate the relative steady state expression levels of ThyA and ThyX, Western blot analysis was performed using ThyA or ThyX antiserum on total protein from the wild-type...

journal_title：FEMS microbiology letters

authors： Cho S,Yang S,Rhie H

更新日期：2012-03-01 00:00:00

abstract：:Morphologically similar species such as Chinese black truffles and Tuber melanosporum were typed by restriction length polymorphism of internal transcribed spacers. This analysis together with sequence comparison revealed the presence of high genetic variability among fruit bodies collected in China. Selection of prim...

journal_title：FEMS microbiology letters

authors： Paolocci F,Rubini A,Granetti B,Arcioni S

更新日期：1997-08-15 00:00:00

abstract：:Mesophilic Aeromonas hydrophila from serotypes O:11 and O:34 grown in a glucose-rich medium produce a capsule that can be seen under light and electron microscopy. The purified capsular polysaccharide has a composition qualitatively similar for strains O:11 and O:34, but quantitatively different. The capsular polysacc...

journal_title：FEMS microbiology letters

authors： Martínez MJ,Simon-Pujol D,Congregado F,Merino S,Rubires X,Tomás JM

更新日期：1995-04-15 00:00:00

abstract：:The clonal relationship of thirty E. coli strains of 0 antigen serotype 06 isolated from human, dog, pig or cow infections were investigated. Two main clones with serotypes 06 : H1 or 06 : H31, H- were identified. Isolates from humans, dogs, pigs and cows were found in both clones, indicating that animals are a possib...

journal_title：FEMS microbiology letters

authors： Cherifi A,Contrepois M,Picard B,Goullet P,Orskov I,Orskov F,De Rycke J

更新日期：1991-05-15 00:00:00

abstract：:Xylella fastidiosa is the causal agent of economically important plant diseases, including citrus variegated chlorosis and Pierce's disease. Hitherto, there has been no information on the molecular mechanisms controlling X. fastidiosa-plant interactions. To determine whether predicted open reading frames (ORFs) encodi...

journal_title：FEMS microbiology letters

authors： Scarpari LM,Lambais MR,Silva DS,Carraro DM,Carrer H

更新日期：2003-05-16 00:00:00

abstract：:We present a method to directly characterize the yeast diversity present in wine fermentations by employing denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 26S ribosomal RNA (rRNA) genes. PCR-DGGE of a portion of the 26S rRNA gene was shown to distinguish most yeast genera a...

journal_title：FEMS microbiology letters

authors： Cocolin L,Bisson LF,Mills DA

更新日期：2000-08-01 00:00:00

abstract：:Chromohalobacter marismortui VH1 was screened for its ability to utilise organosulfonate compounds at a range of NaCl concentrations. Only aminomethane sulfonate, of seven sulfonates tested, was utilised. Length of lag phase during growth on aminomethane sulfonate, as either nitrogen and/or sulfur source, increased wi...

journal_title：FEMS microbiology letters

authors： Ternan NG,McMullan G

更新日期：2002-01-22 00:00:00

abstract：:High quality Fourier transform infrared (FTIR) spectra were acquired from living Micrasterias hardyi cells maintained in an IR transparent flow-through cell using a FTIR microscope coupled to a synchrotron light source. Spectral maps of living, nutrient-replete cells showed band intensities consistent with the known l...

journal_title：FEMS microbiology letters

authors： Heraud P,Wood BR,Tobin MJ,Beardall J,McNaughton D

更新日期：2005-08-15 00:00:00

abstract：:Naturally occurring antimicrobial cationic polypeptides play a major role in innate and adaptive immunity. These polypeptides are found to be either linear and unstructured or structured through disulfide bonds. Among the structured antimicrobial polypeptides, defensins comprise a family of cysteine-rich cationic poly...

journal_title：FEMS microbiology letters

authors： Raj PA,Dentino AR

更新日期：2002-01-02 00:00:00

abstract：:DNA microarray technology was used to survey changes in gene expression in Pseudomonas fluorescens after mitomycin C treatment. As expected, genes associated with the SOS response were upregulated, such as those encoding the recombination protein RecA, DNA repair protein RecN, excinuclease ABC subunit A UvrA, and the ...

journal_title：FEMS microbiology letters

authors： Jin H,Retallack DM,Stelman SJ,Hershberger CD,Ramseier T

更新日期：2007-04-01 00:00:00

abstract：:Yeast two-hybrid and Far Western analyses were used to detect interactions between Bacillus subtilis spores' nutrient germinant receptor proteins and proteins encoded by the spoVA operon, all of which are involved in spore germination and located in the spores' inner membrane. These analyses indicated that two subunit...

journal_title：FEMS microbiology letters

authors： Vepachedu VR,Setlow P

更新日期：2007-09-01 00:00:00

abstract：:Trehalase was studied in Schizosaccharomyces pombe cells growing vegetatively on minimal medium and in sporulating cultures. Acid trehalase activity, measured at pH 4.2, was absent in vegetative cells and occurred only in asci, indicating that this activity represented the sporulation-specific trehalase reported previ...

journal_title：FEMS microbiology letters

authors： De Virgilio C,Müller J,Boller T,Wiemken A

更新日期：1991-11-01 00:00:00

abstract：:Cholera epidemics caused by Vibrio cholerae 01 continue to represent a major public health concern in many developing countries. A rapid and simple test kit for the detection of V. cholerae 01 has been developed. The kit, CholeraScreen is a monoclonal antibody-based, co-agglutination test and is used directly with sto...

journal_title：FEMS microbiology letters

authors： Colwell RR,Hasan JA,Huq A,Loomis L,Siebeling RJ,Torres M,Galvez S,Islam S,Tamplin MT,Bernstein D

更新日期：1992-10-15 00:00:00

abstract：:Helicobacter pylori ATCC43504 responds chemotactically to aspartic acid and serine, but not to arginine, nor to sodium bicarbonate. In contrast, H. pylori ATCC700392 (strain 26695) shows chemotaxis to all four attractants. Open reading frame HP0099 from H. pylori 26695 is predicted to encode one of three methyl-accept...

journal_title：FEMS microbiology letters

authors： Cerda O,Rivas A,Toledo H

更新日期：2003-07-29 00:00:00

abstract：:Two-component systems are widespread in nature and constitute the most common mechanism of transmembrane signal transduction in bacteria. Recently, a functionally active two-component system consisting of malS and malR genes possibly involved in the expression of a C4-dicarboxylate transporter system (dctAB operon) wa...

journal_title：FEMS microbiology letters

authors： Papa R,Rippa V,Duilio A

更新日期：2009-06-01 00:00:00

abstract：:Peptidoglycan hydrolases are an effective new source of antimicrobials. A chimeric fusion protein of the Ply187 endopeptidase domain and LysK SH3b cell wall-binding domain is a potent agent against Staphylococcus aureus in four functional assays. ...

journal_title：FEMS microbiology letters

authors： Mao J,Schmelcher M,Harty WJ,Foster-Frey J,Donovan DM

更新日期：2013-05-01 00:00:00

abstract：:Matrix metalloproteinases (MMPs) play pivotal roles in inflammatory diseases including chronic periodontitis. The effects of Prevotella intermedia, a major periodontal pathogen, on MMP-9 production in primary human periodontal ligament (hPDL) cells were examined in the present study. MMP-9 mRNA expression was measured...

journal_title：FEMS microbiology letters

authors： Guan SM,Shu L,Fu SM,Liu B,Xu XL,Wu JZ

更新日期：2008-06-01 00:00:00

abstract：:Hemerythrins are oxygen-binding proteins found in the body fluids and tissues of certain invertebrates. Oxygen is bound at a nonheme iron centre consisting of two oxo-bridged iron atoms bound to a characteristic set of conserved histidine: aspartate and glutamate residues with the motifs H-HxxxE-HxxxH-HxxxxD. It has r...

journal_title：FEMS microbiology letters

authors： French CE,Bell JM,Ward FB

更新日期：2008-02-01 00:00:00

abstract：:Cell walls of Microcystis sp. PCC 7806 were purified from cell homogenates by sucrose density centrifugation and Triton X-100 extraction. The outer membrane contained carotenoids, two major peptidoglycan-associated proteins (Mr 49,000 and 52,000), and lipopolysaccharide (LPS) as indicated by the presence of 3-hydroxy ...

journal_title：FEMS microbiology letters

authors： Jürgens UJ,Martin C,Weckesser J

更新日期：1989-11-01 00:00:00

abstract：:Because of an association of human neuroborreliosis with the development of an antibody response against an antigen in neural tissue that cross-reacts with an epitope on the flagellin protein of Borrelia burgdorferi, C3H transgenic mice were created that expressed the flagellin epitope (amino acids 213-224) as a fusio...

journal_title：FEMS microbiology letters

authors： Fikrig E,Barthold SW

更新日期：1997-03-15 00:00:00

abstract：:The significance of the chronicled role of the yeast transcription factor Adr1p in regulating ETR1 was examined in wild type and isogenic adr1Delta mutant cells. An ETR1-lacZ reporter construct was used to verify Adr1p-dependent gene expression. On solid glycerol medium containing X-gal, wild-type cells expressing the...

journal_title：FEMS microbiology letters

authors： Gurvitz A

更新日期：2009-08-01 00:00:00

abstract：:Lactic acid bacteria (LAB) have a long history in industrial processes as food starters and biocontrol agents, and also as producers of high-value compounds. Lactic acid, their main product, is among the most requested chemicals because of its multiple applications, including the synthesis of biodegradable plastic pol...

journal_title：FEMS microbiology letters

authors： Tarraran L,Mazzoli R

更新日期：2018-08-01 00:00:00

abstract：:A screening for hydrogen uptake (hup) genes in Rhizobium leguminosarum bv. viciae isolates from different locations within Spain identified no Hup+ strains, confirming the scarcity of the Hup trait in R. leguminosarum. However, five new Hup+ strains were isolated from Ni-rich soils from Italy and Germany. The hup gene...

journal_title：FEMS microbiology letters

authors： Fernández D,Toffanin A,Palacios JM,Ruiz-Argüeso T,Imperial J

更新日期：2005-12-01 00:00:00

abstract：:Streptococcus suis NCTC10234 possesses five srtA homologs: srtA encodes sortase, which anchors surface proteins with an LPXTG motif to the cell wall, while the functions of the other four homologs (the srtBCD cluster and srtE) remain unknown. The genetic organization of the srtA region was found to be conserved in the...

journal_title：FEMS microbiology letters

authors： Osaki M,Takamatsu D,Shimoji Y,Sekizaki T

更新日期：2003-02-28 00:00:00

abstract：:The ascomycete Sclerotinia sclerotiorum is a plant pathogen with a very broad host range. In order to identify and characterize genes involved in S. sclerotiorum infection of Brassica napus (canola), expressed sequence tags (ESTs) were examined from libraries prepared from three tissues: complex appressorium (infectio...

journal_title：FEMS microbiology letters

authors： Sexton AC,Cozijnsen AJ,Keniry A,Jewell E,Love CG,Batley J,Edwards D,Howlett BJ

更新日期：2006-05-01 00:00:00

abstract：:Lyme disease is a multisystemic disorder caused by Borrelia burgdorferi, an invasive spirochete. B. burgdorferi has a predilection for collagenous tissue and one major clinical manifestation of the disease is arthritis. We have identified a collagenolytic activity in B. burgdorferi detergent lysates using iodinated ge...

journal_title：FEMS microbiology letters

authors： Grab DJ,Kennedy R,Philipp MT

更新日期：1996-10-15 00:00:00

abstract：:Helicobacter pylori genomes contain about 30 hop genes that encode outer membrane proteins. Helicobacter pylori hopQ alleles exhibit a high level of genetic diversity, and two families of hopQ alleles have been described. Type I hopQ alleles are found more commonly in cag-positive H. pylori strains from patients with ...

journal_title：FEMS microbiology letters

authors： Loh JT,Torres VJ,Algood HM,McClain MS,Cover TL

更新日期：2008-12-01 00:00:00

abstract：:This study was undertaken to investigate the binding activity of Streptococcus suis serotype 2 to albumin. Using flow cytometry we observed a binding activity of S. suis to albumin for virulent as well as for avirulent isolates. Western immunoblots analysis revealed that a 39-kDa S. suis protein was responsible, at le...

journal_title：FEMS microbiology letters

authors： Quessy S,Busque P,Higgins R,Jacques M,Dubreuil JD

更新日期：1997-02-15 00:00:00

abstract：:Microbial competition for carbon sources is a primary determinant of rhizosphere ecology. We employed the PCR to examine the population fluctuations of a symbiotic nitrogen-fixing bacterium (Rhizobium etli) during the first 11 days following inoculation of Phaseolus vulgaris seedlings grown in the presence or absence ...

journal_title：FEMS microbiology letters

authors： Petersen DJ,Srinivasan M,Chanway CP

更新日期：1996-09-01 00:00:00

abstract：:The low heat resistance (D100 = 0.554 min, z = 13.4 degrees C) of dormant lysozyme-sensitized spores of Bacillus sphaericus 9602 was correlated with a low protoplast wet density (1.305 g/ml) equivalent to a high protoplast water content (61.0%, wet weight basis). These values for these unusual spores were consistent w...

journal_title：FEMS microbiology letters

authors： Beaman TC,Pankratz HS,Gerhardt P

更新日期：1989-03-01 00:00:00