Current status of defensins and their role in innate and adaptive immunity.

abstract：:The small noncoding RNA RyhB is a regulator of iron homeostasis in Escherichia coli. During iron limitation, it downregulates the expression of a number of iron-containing proteins, including enzymes of the tricarboxylic acid cycle and the respiratory chain. Because this infers a potential for RyhB to limit energy met...

journal_title：FEMS microbiology letters

authors： Bollinger CJ,Kallio PT

更新日期：2007-10-01 00:00:00

abstract：:The 0104 antigen (lipopolysaccharide, LPS) of Escherichia coli has an acidic O specific polysaccharide. From the aqueous phase of a phenol water extraction of E. coli O104: K-, a fraction was obtained by ultracentrifugation and Cetavlon precipitation of the supernatant, which was enriched in long-chain LPS. Compositio...

journal_title：FEMS microbiology letters

authors： Kogan G,Jann B,Jann K

更新日期：1992-03-01 00:00:00

abstract：:The emergence of antibiotic resistance has necessitated new therapeutic approaches for combating persistent bacterial infection. An alternative approach is regulation of bacterial virulence instead of growth suppression, which can readily lead to drug resistance. The virulence of the opportunistic human pathogen Pseud...

journal_title：FEMS microbiology letters

authors： Lee JH,Kim YG,Cho MH,Kim JA,Lee J

更新日期：2012-04-01 00:00:00

abstract：:A gene library of Neisseria meningitidis B15 P1.16 DNA was established in lambda Zap II and clones containing DNA encoding transferrin binding protein 1 (TBP-1) identified following hybridisation with a 63-bp DNA probe based on the codon assignment for the first 21 N-terminal amino acids of TBP-1. Sequencing of the cl...

journal_title：FEMS microbiology letters

authors： Palmer HM,Powell NB,Ala'Aldeen DA,Wilton J,Borriello SP

更新日期：1993-06-15 00:00:00

abstract：:Two fungal isolates, formerly classified as Verticillium alboatrum and proposed as forming the basis of a new sub-group ('Group 2') within the species, have been shown to be non-pathogenic to known hosts of V. alboatrum and, on the basis of molecular evidence, to be closely related to Verticillium psalliotae and Verti...

journal_title：FEMS microbiology letters

authors： Carder JH,Barbara DJ

更新日期：1999-01-01 00:00:00

abstract：:Academic work at different career stages has changed and a broadened portfolio of expertise enables academics to adapt, maintain and advance their career. Development related to research activity is naturally driven by methodology and technology. Institutions and peers largely support development in the contexts of di...

journal_title：FEMS microbiology letters

authors： Fahnert B

更新日期：2015-10-01 00:00:00

abstract：:Lineage-specific genes (LSGs) are defined as genes with sequences that are not significantly similar to those in any other lineage. LSGs have been proposed, and sometimes shown, to have significant effects in the evolution of biological function. In this study, two sets of Hanseniaspora spp. LSGs were identified by co...

journal_title：FEMS microbiology letters

authors： Chen K,Tian Z,Chen P,He H,Jiang F,Long CA

更新日期：2020-06-01 00:00:00

abstract：:Five transposon mutants of Francisella novicida were isolated that are compromised in their ability to grow in mouse macrophages in vitro. Sequence analysis of the DNA flanking the transposon insertions identified the genes that were interrupted in these mutants. One of the inactivated loci corresponds to the Francise...

journal_title：FEMS microbiology letters

authors： Gray CG,Cowley SC,Cheung KK,Nano FE

更新日期：2002-09-24 00:00:00

abstract：:Porphyromonas gingivalis produces a trypsin-like enzyme, Protease I, which is thought to be an important virulence determinant of the organism in adult periodontal disease. Protease I is transiently inhibited by physiological inhibitors of human thrombin. The aim of the present work was to establish whether Protease I...

journal_title：FEMS microbiology letters

authors： Curtis MA,Macey M,Slaney JM,Howells GL

更新日期：1993-06-15 00:00:00

abstract：:The sequences of the epidermolytic toxins and V8 serine proteinase share about 25% identity, including the catalytic triad at the proteinase active centre. Here we have altered the putative ETA active-site serine-195 to glycine by site-directed mutagenesis. No epidermolytic activity was detected when up to 100-fold gr...

journal_title：FEMS microbiology letters

authors： Redpath MB,Foster TJ,Bailey CJ

更新日期：1991-06-15 00:00:00

abstract：:Interest in endophytes as natural sources for new medicines was inspired by the discovery of paclitaxel-producing endophytic fungi. This study investigated the anti-cancer activity of extracts of endophytes isolated from two Australian plants, Eremophila longifolia (EL) and Eremophila maculata (EM). Endophytes were is...

journal_title：FEMS microbiology letters

authors： Zaferanloo B,Pepper SA,Coulthard SA,Redfern CPF,Palombo EA

更新日期：2018-05-01 00:00:00

abstract：:Bacillus sp. strain CS93, which was previously isolated from Pozol, was previously shown to produce iturin A, bacilysin and chlorotetaine. To investigate the biosynthetic mechanism of chlorotetaine production, the bac genes were amplified from genomic DNA of Bacillus sp. CS93 by PCR and sequenced. The genes bacABCDE w...

journal_title：FEMS microbiology letters

authors： Moran S,Robertson K,Paradisi F,Rai DK,Murphy CD

更新日期：2010-03-01 00:00:00

abstract：:An accurate monitoring of disease progression is important to evaluate disease susceptibility phenotypes. Over the years, Arabidopsis thaliana has become the model species to serve as a host in plant-pathogen interactions. Despite the efforts to study genetic mechanisms of host defense, little efforts are made for a t...

journal_title：FEMS microbiology letters

authors： Brouwer M,Lievens B,Van Hemelrijck W,Van den Ackerveken G,Cammue BP,Thomma BP

更新日期：2003-11-21 00:00:00

abstract：:In Bacillus subtilis, the yoxA and dacC genes were proposed to form an operon. The yoxA gene was overexpressed in Escherichia coli and its product fused to a polyhistidine tag was purified. An aldose-1-epimerase or mutarotase activity was measured with the YoxA protein that we propose to rename as GalM by analogy with...

journal_title：FEMS microbiology letters

authors： Duez C,Zervosen A,Teller N,Melkonian R,Banzubazé E,Bouillenne F,Luxen A,Frère JM

更新日期：2009-11-01 00:00:00

abstract：:The Gram-positive bacterium Streptococcus gordonii has been genetically engineered to allow the simultaneous expression of two heterologous proteins at the cell surface. A family of recombinant streptococci displaying two different antigens was constructed. All the strains were genetically stable and expressed both pr...

journal_title：FEMS microbiology letters

authors： Maggi T,Spinosa M,Ricci S,Medaglini D,Pozzi G,Oggioni MR

更新日期：2002-04-23 00:00:00

abstract：:Streptococcus mutans, the etiologic agent of dental caries in humans, is considered a dominating force in the oral microbiome due to its highly-evolved propensity for survival. The oral pathogen encodes an elaborate array of regulatory elements, including the carbon catabolite-responsive regulator, CcpA, a global regu...

journal_title：FEMS microbiology letters

authors： Lindsay EL,Faustoferri RC,Quivey RG

更新日期：2021-01-16 00:00:00

abstract：:Many antibiotic-producing actinomycetes possess at least one ABC (ATP-binding cassette) transporter which forms part of the antibiotic biosynthetic pathway and in most cases confers resistance to the drug in an heterologous host. Three types of antibiotic ABC transporters have been so far described in producer organis...

journal_title：FEMS microbiology letters

authors： Méndez C,Salas JA

更新日期：1998-01-01 00:00:00

abstract：:A gene expression system for both Bacillus subtilis and Escherichia coli was developed. The expression vector, pHASH102, produces any combination of promoter and open reading frame to be expressed based on the T-extended cloning method. Because the pHASH series vectors are designed to shuttle between the genome and a ...

journal_title：FEMS microbiology letters

authors： Ohashi Y,Ohshima H,Tsuge K,Itaya M

更新日期：2003-04-11 00:00:00

abstract：:In the present study, the homology relationships among fragments amplified by ERIC (Enterobacterial Repetitive Intergenic Consensus) and REP (Repetitive Extragenic Palindromic) primers in Drechslera avenae and Stemphylium solani causing leaf blotch and black spot of oats and leaf blight of cotton, respectively, were i...

journal_title：FEMS microbiology letters

authors： Mehta A,Mehta YR,Rosato YB

更新日期：2002-05-21 00:00:00

abstract：:The aim of this study was to illustrate the relative pervasiveness of Borderline Oxacillin Resistant Staphylococcus aureus (BORSA) and Methicillin-Resistant Staphylococcus aureus (MRSA) in community and food of animal origin and their relationship with other genetic determinants. Staphylococcus aureus isolates were su...

journal_title：FEMS microbiology letters

authors： Zehra A,Gulzar M,Singh R,Kaur S,Gill JPS

更新日期：2020-12-22 00:00:00

abstract：:Two Pseudomonas aeruginosa genes encoding the enzymes 1-deoxy-D-xylulose 5-phosphate (DXP) synthase and DXP reductoisomerase, both involved in the mevalonate-independent biosynthesis of isoprenoids, have been expressed as recombinant enzymes in Escherichia coli. The purified P. aeruginosa DXP reductoisomerase was inhi...

journal_title：FEMS microbiology letters

authors： Altincicek B,Hintz M,Sanderbrand S,Wiesner J,Beck E,Jomaa H

更新日期：2000-09-15 00:00:00

abstract：:To tackle antimicrobial resistance (AMR) is of outmost importance for the general population to understand the severity and the relevance of different routes of transmission. Respondents of different age groups, educational and occupational backgrounds, area of living, diet and household composition participated in an...

journal_title：FEMS microbiology letters

authors： Alexa Oniciuc EA,Likotrafiti E,Garre A,Ruiz L,Prieto M,Alvarez-Ordóñez A

更新日期：2019-09-01 00:00:00

abstract：:The quorum quenching (QQ) activity of endophytic bacteria associated with medicinal plants was explored. Extracts of the Gram-negative Enterobacter sp. CS66 possessed potent N-acylhomoserine lactone (AHL) hydrolytic activity in vitro. Using degenerate primers, we PCR-amplified an open reading frame (denoted aiiE) from...

journal_title：FEMS microbiology letters

authors： Shastry RP,Dolan SK,Abdelhamid Y,Vittal RR,Welch M

更新日期：2018-05-01 00:00:00

abstract：:The plant antimicrobial peptide MiAMP1 from Macadamia integrifolia and the yeast killer toxin peptide WmKT from Williopsis mrakii are structural homologues. Comparative studies of yeast mutants were performed to test their sensitivity to these two antimicrobial peptides. No differences in susceptibility to MiAMP1 were...

journal_title：FEMS microbiology letters

authors： Stephens C,Kazan K,Goulter KC,Maclean DJ,Manners JM

更新日期：2005-02-01 00:00:00

abstract：:The est gene encoding an esterase from Acinetobacter lwoffii RAG-1 was cloned into E. coli under the control of the PL promoter of the phage lambda. The N-terminal sequence of the first 20 amino acids of the heterologous expressed esterase corresponded to that obtained from the nucleotide sequence. Antibodies prepared...

journal_title：FEMS microbiology letters

authors： Alon RN,Gutnick DL

更新日期：1993-09-15 00:00:00

abstract：:We developed an improved HEp-2 cell assay method for the detection of Bacillus cereus toxin, which affects the proliferation of HEp-2 cells. The cytostatic toxin was stable upon exposure to heat, pH 2, pH 11 and trypsin, which suggests it is an emetic. Using the HEp-2 cell assay, we examined the distribution and conta...

journal_title：FEMS microbiology letters

authors： Mikami T,Horikawa T,Murakami T,Matsumoto T,Yamakawa A,Murayama S,Katagiri S,Shinagawa K,Suzuki M

更新日期：1994-06-01 00:00:00

abstract：:DNA sequence analysis of the nadA gene of Ehrlichia chaffeensis revealed a 942 bp open reading frame with the capacity to encode 314 amino acids. The amino acid sequence of the E. chaffeensis quinolinate synthetase A (NAD/A) has 53.6% identity and 82% similarity to the NAD A of the cyanelle of Cyanophora paradoxa. Por...

journal_title：FEMS microbiology letters

authors： Yu XJ,Walker DH

更新日期：1997-09-01 00:00:00

abstract：:Trinitrotoluene (TNT) is an explosive chemical generally used for military, civil and industrial purposes. Therefore, TNT residue can be found in soil and ground water as an environmental pollutant. The environmental control of TNT pollution has become a critical issue because of its potential toxicity and carcinogeni...

journal_title：FEMS microbiology letters

authors： Kao CM,Wei SF,Chen SC,Yao CL,Ma C,Chien CC

更新日期：2018-01-01 00:00:00

abstract：:Bacterial pathogens face constant challenges from DNA-damaging agents generated by host phagocytes. Although Borrelia burgdorferi appears to have much fewer DNA repair enzymes than pathogens with larger genomes, it does contain homologues of uvrA and uvrB (subunits A and B of excinuclease ABC). As a first step to expl...

journal_title：FEMS microbiology letters

authors： Sambir M,Ivanova LB,Bryksin AV,Godfrey HP,Cabello FC

更新日期：2011-04-01 00:00:00

abstract：:DNA polymerase from the archaeon Pyrococcus abyssi strain Orsay was expressed in Escherichia coli. The recombinant DNA polymerase (Pab) was purified to homogeneity by heat treatment followed by 5 steps of chromatography and characterized for PCR applications. Buffer optimization experiments indicated that Pab PCR perf...

journal_title：FEMS microbiology letters

authors： Dietrich J,Schmitt P,Zieger M,Preve B,Rolland JL,Chaabihi H,Gueguen Y

更新日期：2002-11-19 00:00:00