Repression of the TreR transcriptional regulator in Streptococcus mutans by the global regulator, CcpA.

Abstract:

:Streptococcus mutans, the etiologic agent of dental caries in humans, is considered a dominating force in the oral microbiome due to its highly-evolved propensity for survival. The oral pathogen encodes an elaborate array of regulatory elements, including the carbon catabolite-responsive regulator, CcpA, a global regulator key in the control of sugar metabolism and in stress tolerance response mechanisms. The recently characterized trehalose utilization operon, integral for the catabolism of the disaccharide trehalose, is controlled by a local regulator, TreR, which has been implicated in a number of cellular functions outside of trehalose catabolism. Electrophoretic mobility shift assays demonstrated that CcpA bound a putative cre site in the treR promoter. Loss of ccpA resulted in elevated expression of treR in cultures of the organism grown in glucose or trehalose, indicating that CcpA not only acts as a repressor of trehalose catabolism genes, but also the local regulator. The loss of both CcpA and TreR in S. mutans resulted in an impaired growth rate and fitness response, supporting the hypothesis that these regulators are involved in carbon catabolism control and in induction of components of the organism's stress response.

journal_name

FEMS Microbiol Lett

authors

Lindsay EL,Faustoferri RC,Quivey RG

doi

10.1093/femsle/fnab004

subject

Has Abstract

pub_date

2021-01-16 00:00:00

eissn

0378-1097

issn

1574-6968

pii

6102557

pub_type

杂志文章
  • Conformational plasticity of molecular chaperones involved in periplasmic and outer membrane protein folding.

    abstract::How proteins reach their native conformation and location has been a major question of biology during the last 50 years. To counterbalance protein misfolding and the accumulation of aggregation products, a complex network of chaperones and proteases takes care of protein quality control in the cell. Such a chaperone n...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fny121

    authors: Mas G,Hiller S

    更新日期:2018-07-01 00:00:00

  • The emergence of colistin-resistant Escherichia coli in chicken meats in Nepal.

    abstract::The emergence and dissemination of colistin resistance among Gram-negative bacteria is a global problem. We initiated a surveillance of colistin-resistant and -susceptible Escherichia coli in raw meats from chicken in Nepal. A total of 180 meat samples were collected; from these, 60 E. coli strains were isolated (33.3...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz237

    authors: Joshi PR,Thummeepak R,Leungtongkam U,Pooarlai R,Paudel S,Acharya M,Dhital S,Sitthisak S

    更新日期:2019-10-01 00:00:00

  • Isolation and sequence of a 2-kbp miniplasmid from Bacillus thuringiensis var. kurstaki HD-3a3b: relationship with miniplasmids of other B. thuringiensis strains.

    abstract::The miniplasmid profiles of 18 Bacillus thuringiensis strains belonging to 8 different serotypes were determined using an alkaline hydrolysis method for isolation of low molecular mass plasmids. Nearly all the strains contained covalently closed circular (CCC) DNA species ranging from 2 to 5 species per strain and fro...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90641-z

    authors: Marin R,Tanguay RM,Valéro J,Letarte R,Bellemare G

    更新日期:1992-07-15 00:00:00

  • Phage vB_BsuP-Goe1: the smallest identified lytic phage of Bacillus subtilis.

    abstract::Currently, interest in phage science is on the rise again as this subject remains largely unexplored, and its potential diversity mainly untapped. Here, we present phage vB_BsuP-Goe1, a new isolate that infects Bacillus subtilis The phage forms round plaques with a matt outline on agar plates containing a B. subtilis ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnw208

    authors: Willms IM,Hertel R

    更新日期:2016-10-01 00:00:00

  • Two types of ion channel formation of tolaasin, a Pseudomonas peptide toxin.

    abstract::Tolaasin is a peptide toxin produced by Pseudomonas tolaasii and causes brown blotch disease of the cultivated mushrooms. Two types of ion channels were identified by the incorporation of tolaasin into lipid bilayer. The slope conductance of type 1 channel measured in the buffer containing 100 mM KCl was 150 pS with a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00182-4

    authors: Cho KH,Kim YK

    更新日期:2003-04-25 00:00:00

  • Evaluation of the catalase promoter for expressing the alkaline xylanase gene (alx) in Aspergillus niger.

    abstract::Aspergillus niger represents a promising host for the expression of recombinant proteins, but only a few expression systems are available for this organism. In this study, the inducible catalase promoter (PcatR) from A. niger was characterized. For this, constructs were developed and checked for the expression of the ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02454.x

    authors: Sharma R,Katoch M,Govindappa N,Srivastava PS,Sastry KN,Qazi GN

    更新日期:2012-02-01 00:00:00

  • Isolation and preliminary characterisation of twenty-five temperature-sensitive mutants of mouse cytomegalovirus.

    abstract::To study the pathogenicity of mouse cytomegalovirus (MCMV) and to identify virulence determinants, we have isolated and phenotypically characterised 25 temperature-sensitive (ts) mutants. Six of these (tsm9, tsm13, tsm20, tsm22, tsm28 and tsm30) failed to replicate in mice and were avirulent. Five mutants (tsm14, tsm1...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06523.x

    authors: Akel HM,Sweet C

    更新日期:1993-11-01 00:00:00

  • General and kinetic properties of endoglucanase from Aspergillus niger.

    abstract::Endoglucanase of Aspergillus niger AS-101 was partially purified by ammonium sulphate fractionation and molecular sieving on Sephadex G-200. The enzyme was found to be unstable on storage, however, it received some protection on the addition of BSA, glycerol or sodium azide. Glycerol also protected the enzyme from ina...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90060-4

    authors: Singh A,Agrawal AK,Abidi AB,Darmwal NS

    更新日期:1990-09-01 00:00:00

  • Characterization of a periplasmic peptidyl-prolyl cis-trans isomerase in Erwinia chrysanthemi.

    abstract::The main determinant of the plant pathogen Erwinia chrysanthemi virulence is the production of extracellular enzymes, mainly pectate lyases. Adjacent to a pectate lyase encoding locus, we identified the gene rotA supposed to encode a folding catalyst. Overproduction of the protein and assay of activity using a synthet...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1997.tb12753.x

    authors: Pissavin C,Hugouvieux-Cotte-Pattat N

    更新日期:1997-12-01 00:00:00

  • Increased expression and hotspot mutations of the multidrug efflux transporter, CDR1 in azole-resistant Candida albicans isolates from vaginitis patients.

    abstract::The aims of our research were to investigate the gene expression of the multidrug efflux transporter, CDR1 and the major drug facilitator superfamily transporter, MDR1 gene in azole drug-resistant Candida albicans and Candida glabrata clinical isolates recovered from vaginitis patients; and to identify hotspot mutatio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.06.036

    authors: Looi CY,D' Silva EC,Seow HF,Rosli R,Ng KP,Chong PP

    更新日期:2005-08-15 00:00:00

  • The immunosuppressive drug leflunomide affects mating-pheromone response and sporulation by different mechanisms in Saccharomyces cerevisiae.

    abstract::Leflunomide (LFM) is a novel anti-inflammatory and immunosuppressive drug, and inhibits the growth of cytokine-stimulated lymphoid cells in vitro. The effect of LFM on haploid and diploid cells of Saccharomyces cerevisiae was investigated to elucidate the molecular mechanism of action of the drug. Using a halo assay, ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09319.x

    authors: Fujimura H

    更新日期:2000-10-01 00:00:00

  • Functional conservation between the argininosuccinate lyase of the archaeon Methanococcus maripaludis and the corresponding bacterial and eukaryal genes.

    abstract::The argH gene encoding argininosuccinate lyase (ASL) of Methanococcus maripaludis was cloned on a 4.7-kb HindIII genomic fragment. The gene is preceded by a short open reading frame (ORF149), which encodes a polypeptide with an unknown function. The two genes are co-transcribed. The ASL of M. maripaludis shares a high...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13507.x

    authors: Cohen-Kupiec R,Kupiec M,Sandbeck K,Leigh JA

    更新日期:1999-04-01 00:00:00

  • A class II hydrophobin gene, Trhfb3, participates in fungal asexual development of Trichoderma reesei.

    abstract::Hydrophobin proteins originate from filamentous fungi, which are able to self-assemble at water-air interfaces. Hydrophobins have multiple functions in fungal growth and development. In this study, the function of the Trhfb3 gene encoding a class II hydrophobin was characterized in Trichoderma reesei. The null mutant ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnw297

    authors: He R,Li C,Feng J,Zhang D

    更新日期:2017-04-01 00:00:00

  • Cloning and partial sequence of transferrin-binding protein 2 of Neisseria meningitidis using a novel method: twin N-terminal PCR.

    abstract::The genes encoding transferrin-binding proteins (TBPs) 1 and 2 of Neisseria meningitidis and N. gonorrhoeae were used as model loci in a novel method of cloning (twin N-terminal polymerase chain reaction; TNT-PCR) involving amplification between the 5' ends of two genes. Primers were based on N-terminal amino-acid seq...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(93)90354-5

    authors: Wilton J,Ala'Aldeen D,Palmer HM,Borriello SP

    更新日期:1993-02-15 00:00:00

  • Biochemical characterization of alpha-amylase from the yeast Cryptococcus flavus.

    abstract::During our screening of amylolytic microorganisms from Brazilian fruits, we isolated a yeast strain classified as Cryptococcus flavus. When grown on starch-containing medium this strain exhibited the highest amylase production after 24 h of cultivation. The extracellular amylase from C. flavus was purified from the cu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00955-8

    authors: Wanderley KJ,Torres FA,Moraes LM,Ulhoa CJ

    更新日期:2004-02-16 00:00:00

  • Acanthamoeba sp. promotes the survival and growth of Acinetobacter baumanii.

    abstract::Acinetobacter baumanii, which may be found in water, is an important emerging hospital-acquired pathogen. Free-living amoebae can be recovered from the same water networks, and it has been shown that these protozoa may support the growth of other bacteria. In this paper, we have studied potential relationships between...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02261.x

    authors: Cateau E,Verdon J,Fernandez B,Hechard Y,Rodier MH

    更新日期:2011-06-01 00:00:00

  • Inhibitory effect of lipopolysaccharide on apoptotic cell death in macrophages infected with Actinobacillus actinomycetemcomitans.

    abstract::We have reported that the macrophage-like cell line J774.1, when infected with the periodontopathic bacterium Actinobacillus actinomycetemcomitans, undergoes apoptosis. In this study, we examined whether stimulation of J774.1 cells with lipopolysaccharide (LPS) before the infection affects the subsequent apoptosis. Cy...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13622.x

    authors: Muro M,Nakashima K,Tomioka J,Kato S,Nonaka K,Yoshida T,Inoue M,Nishihara T,Kowashi Y

    更新日期:1999-06-15 00:00:00

  • Isolation of genes encoding beta-D-xylanase, beta-D-xylosidase and alpha-L-arabinofuranosidase activities from the rumen bacterium Prevotella ruminicola B1(4).

    abstract::Prevotella ruminicola B1(4) is a strictly anaerobic, Gram-negative, polysaccharide-degrading rumen bacterium. Xylanase activity in this strain was found to be inducible, the specific activity of cells grown on xylan being increased at least 20-fold by comparison with cells grown on glucose. Ten bacteriophage clones ex...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07349.x

    authors: Gasparic A,Marinsek-Logar R,Martin J,Wallace RJ,Nekrep FV,Flint HJ

    更新日期:1995-01-15 00:00:00

  • Optimized conditions for the fermentation of Helicobacter pylori and production of vacuolating cytotoxin.

    abstract::We report here improvements to the growth media and fermentation conditions which result in a substantial increase of Helicobacter pylori growth and in the enhanced production of vacuolating cytotoxin. Addition of glucose to the medium resulted in the increase of cell yield, cell viability and a significant improvemen...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb07261.x

    authors: Marchini A,Massari P,Manetti R,Olivieri R

    更新日期:1994-11-15 00:00:00

  • Impact of land use history on the arbuscular mycorrhizal fungal diversity in arid soils of Argentinean farming fields.

    abstract::Arbuscular mycorrhizal fungi (AMF) are a key soil functional group, with an important potential to increase crop productivity and sustainable agriculture including food security. However, there is clear evidence that land uses, crop rotations and soil features affect the AMF diversity and their community functioning i...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnaa114

    authors: Ontivero RE,Voyron S,Allione LVR,Bianco P,Bianciotto V,Iriarte HJ,Lugo MA,Lumini E

    更新日期:2020-07-01 00:00:00

  • Lactococcus piscium sp. nov. a new Lactococcus species from salmonid fish.

    abstract::Chemical and molecular taxonomic studies were performed on a representative strain of some lactic acid bacteria of unknown taxonomic position isolated from salmonid fish. The results demonstrate that the fish bacterium represents a new species of the genus Lactococcus for which the name Lactococcus piscium sp. nov. is...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90134-c

    authors: Williams AM,Fryer JL,Collins MD

    更新日期:1990-03-01 00:00:00

  • vpsA- and luxO-independent biofilms of Vibrio cholerae.

    abstract::The natural life cycle of Vibrio cholerae involves the transitioning of cells between different environmental surfaces such as the chitinous shell of Crustaceae and the epithelial layer of the human intestine. Previous studies using static biofilm systems showed a strict dependence of biofilm formation on the vps and ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00884.x

    authors: Müller J,Miller MC,Nielsen AT,Schoolnik GK,Spormann AM

    更新日期:2007-10-01 00:00:00

  • Genetic investigation within Lactococcus garvieae revealed two genomic lineages.

    abstract::The diversity of a collection of 49 Lactococcus garvieae strains, including isolates of dairy, fish, meat, vegetable and cereal origin, was explored using a molecular polyphasic approach comprising PCR-ribotyping, REP and RAPD-PCR analyses and a multilocus restriction typing (MLRT) carried out on six partial genes (at...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/j.1574-6968.2012.02591.x

    authors: Ferrario C,Ricci G,Borgo F,Rollando A,Fortina MG

    更新日期:2012-07-01 00:00:00

  • Towards biobased industry: acetate as a promising feedstock to enhance the potential of microbial cell factories.

    abstract::A broad range of different chemical and pharmaceutical compounds have been produced in microbial cell factories. To compete with traditional crude oil based production processes, the use of complex alternative raw materials such as lignocellulosic biomass, waste streams and utilization of CO2 in gas fermentations has ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fny226

    authors: Novak K,Pflügl S

    更新日期:2018-10-01 00:00:00

  • Draft genome sequences of three Holospora species (Holospora obtusa, Holospora undulata, and Holospora elegans), endonuclear symbiotic bacteria of the ciliate Paramecium caudatum.

    abstract::We present draft genome sequences of three Holospora species, hosted by the ciliate Paramecium caudatum; that is, the macronucleus-specific H. obtusa and the micronucleus-specific H. undulata and H. elegans. We investigate functions of orthologous core genes conserved across the three Holospora species, which may be e...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12577

    authors: Dohra H,Tanaka K,Suzuki T,Fujishima M,Suzuki H

    更新日期:2014-10-01 00:00:00

  • Nucleotide sequence and structural organization of Yersinia pestis insertion sequence IS100.

    abstract::Insertion sequence IS100 was localized on a 9.5-kb plasmid of Yersinia pestis and was shown to be specific for Y. pestis and serotype I strains of Y. pseudotuberculosis. The nucleotide sequence of IS100 isolated from this plasmid was determined. The element, which was flanked by 5-bp direct repeats, contained 1953 bp ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb07111.x

    authors: Podladchikova ON,Dikhanov GG,Rakin AV,Heesemann J

    更新日期:1994-09-01 00:00:00

  • Lactobacillus fermentum 3872 genome sequencing reveals plasmid and chromosomal genes potentially involved in a probiotic activity.

    abstract::In this report we describe a Lactobacillus fermentum 3872 plasmid (pLF3872) not previously found in any other strain of this species. The analysis of the complete sequence of this plasmid revealed the presence of a gene encoding a large collagen-binding protein (CBP), as well as the genes responsible for plasmid maint...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv068

    authors: Lehri B,Seddon AM,Karlyshev AV

    更新日期:2015-06-01 00:00:00

  • Effect of antibiotics on the bacterial population of the rabbit caecum.

    abstract::The effect feeding antibiotics has on the bacterial population of the rabbit caecum was investigated. No changes in total volatile fatty acid production or total bacterial counts were observed compared with nonantibiotic treated controls. However, treatment with chlortetracycline resulted in an increase of propionate ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00746.x

    authors: Abecia L,Fondevila M,Balcells J,Edwards JE,Newbold CJ,McEwan NR

    更新日期:2007-07-01 00:00:00

  • Extracellular polymeric substances responsible for bacterial adhesion onto solid surface.

    abstract::The influence of extracellular polymeric substances (EPS) on bacterial cell adhesion onto solid surfaces was investigated using 27 heterotrophic bacterial strains isolated from a wastewater treatment reactor. Cell adhesion onto glass beads was carried out by the packed-bed method and the results were discussed in term...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00399-9

    authors: Tsuneda S,Aikawa H,Hayashi H,Yuasa A,Hirata A

    更新日期:2003-06-27 00:00:00

  • Heat-inactivation of plasmid-encoded CI857 repressor induces gene expression from Ind- lambda prophage in recombinant Escherichia coli.

    abstract::We have observed significant cell lysis upon temperature up-shift of recombinant Escherichia coli cultures harboring CI857-repressed lambda-based expression vectors. This event, that becomes evident about 30-40 min after the heat shock, takes place when using the lambda promoter system in Ind- lysogenic strains, but n...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13750.x

    authors: Hoffmann F,Arís A,Carbonell X,Rohde M,Corchero JL,Rinas U,Villaverde A

    更新日期:1999-08-15 00:00:00