Repression of the TreR transcriptional regulator in Streptococcus mutans by the global regulator, CcpA.


:Streptococcus mutans, the etiologic agent of dental caries in humans, is considered a dominating force in the oral microbiome due to its highly-evolved propensity for survival. The oral pathogen encodes an elaborate array of regulatory elements, including the carbon catabolite-responsive regulator, CcpA, a global regulator key in the control of sugar metabolism and in stress tolerance response mechanisms. The recently characterized trehalose utilization operon, integral for the catabolism of the disaccharide trehalose, is controlled by a local regulator, TreR, which has been implicated in a number of cellular functions outside of trehalose catabolism. Electrophoretic mobility shift assays demonstrated that CcpA bound a putative cre site in the treR promoter. Loss of ccpA resulted in elevated expression of treR in cultures of the organism grown in glucose or trehalose, indicating that CcpA not only acts as a repressor of trehalose catabolism genes, but also the local regulator. The loss of both CcpA and TreR in S. mutans resulted in an impaired growth rate and fitness response, supporting the hypothesis that these regulators are involved in carbon catabolism control and in induction of components of the organism's stress response.


FEMS Microbiol Lett


Lindsay EL,Faustoferri RC,Quivey RG




Has Abstract


2021-01-16 00:00:00








  • Conformational plasticity of molecular chaperones involved in periplasmic and outer membrane protein folding.

    abstract::How proteins reach their native conformation and location has been a major question of biology during the last 50 years. To counterbalance protein misfolding and the accumulation of aggregation products, a complex network of chaperones and proteases takes care of protein quality control in the cell. Such a chaperone n...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Mas G,Hiller S

    更新日期:2018-07-01 00:00:00

  • The emergence of colistin-resistant Escherichia coli in chicken meats in Nepal.

    abstract::The emergence and dissemination of colistin resistance among Gram-negative bacteria is a global problem. We initiated a surveillance of colistin-resistant and -susceptible Escherichia coli in raw meats from chicken in Nepal. A total of 180 meat samples were collected; from these, 60 E. coli strains were isolated (33.3...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Joshi PR,Thummeepak R,Leungtongkam U,Pooarlai R,Paudel S,Acharya M,Dhital S,Sitthisak S

    更新日期:2019-10-01 00:00:00

  • Isolation and sequence of a 2-kbp miniplasmid from Bacillus thuringiensis var. kurstaki HD-3a3b: relationship with miniplasmids of other B. thuringiensis strains.

    abstract::The miniplasmid profiles of 18 Bacillus thuringiensis strains belonging to 8 different serotypes were determined using an alkaline hydrolysis method for isolation of low molecular mass plasmids. Nearly all the strains contained covalently closed circular (CCC) DNA species ranging from 2 to 5 species per strain and fro...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Marin R,Tanguay RM,Valéro J,Letarte R,Bellemare G

    更新日期:1992-07-15 00:00:00

  • Phage vB_BsuP-Goe1: the smallest identified lytic phage of Bacillus subtilis.

    abstract::Currently, interest in phage science is on the rise again as this subject remains largely unexplored, and its potential diversity mainly untapped. Here, we present phage vB_BsuP-Goe1, a new isolate that infects Bacillus subtilis The phage forms round plaques with a matt outline on agar plates containing a B. subtilis ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Willms IM,Hertel R

    更新日期:2016-10-01 00:00:00

  • Two types of ion channel formation of tolaasin, a Pseudomonas peptide toxin.

    abstract::Tolaasin is a peptide toxin produced by Pseudomonas tolaasii and causes brown blotch disease of the cultivated mushrooms. Two types of ion channels were identified by the incorporation of tolaasin into lipid bilayer. The slope conductance of type 1 channel measured in the buffer containing 100 mM KCl was 150 pS with a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cho KH,Kim YK

    更新日期:2003-04-25 00:00:00

  • Evaluation of the catalase promoter for expressing the alkaline xylanase gene (alx) in Aspergillus niger.

    abstract::Aspergillus niger represents a promising host for the expression of recombinant proteins, but only a few expression systems are available for this organism. In this study, the inducible catalase promoter (PcatR) from A. niger was characterized. For this, constructs were developed and checked for the expression of the ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sharma R,Katoch M,Govindappa N,Srivastava PS,Sastry KN,Qazi GN

    更新日期:2012-02-01 00:00:00

  • Isolation and preliminary characterisation of twenty-five temperature-sensitive mutants of mouse cytomegalovirus.

    abstract::To study the pathogenicity of mouse cytomegalovirus (MCMV) and to identify virulence determinants, we have isolated and phenotypically characterised 25 temperature-sensitive (ts) mutants. Six of these (tsm9, tsm13, tsm20, tsm22, tsm28 and tsm30) failed to replicate in mice and were avirulent. Five mutants (tsm14, tsm1...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Akel HM,Sweet C

    更新日期:1993-11-01 00:00:00

  • General and kinetic properties of endoglucanase from Aspergillus niger.

    abstract::Endoglucanase of Aspergillus niger AS-101 was partially purified by ammonium sulphate fractionation and molecular sieving on Sephadex G-200. The enzyme was found to be unstable on storage, however, it received some protection on the addition of BSA, glycerol or sodium azide. Glycerol also protected the enzyme from ina...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Singh A,Agrawal AK,Abidi AB,Darmwal NS

    更新日期:1990-09-01 00:00:00

  • Characterization of a periplasmic peptidyl-prolyl cis-trans isomerase in Erwinia chrysanthemi.

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Pissavin C,Hugouvieux-Cotte-Pattat N

    更新日期:1997-12-01 00:00:00

  • Increased expression and hotspot mutations of the multidrug efflux transporter, CDR1 in azole-resistant Candida albicans isolates from vaginitis patients.

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Looi CY,D' Silva EC,Seow HF,Rosli R,Ng KP,Chong PP

    更新日期:2005-08-15 00:00:00

  • The immunosuppressive drug leflunomide affects mating-pheromone response and sporulation by different mechanisms in Saccharomyces cerevisiae.

    abstract::Leflunomide (LFM) is a novel anti-inflammatory and immunosuppressive drug, and inhibits the growth of cytokine-stimulated lymphoid cells in vitro. The effect of LFM on haploid and diploid cells of Saccharomyces cerevisiae was investigated to elucidate the molecular mechanism of action of the drug. Using a halo assay, ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fujimura H

    更新日期:2000-10-01 00:00:00

  • Functional conservation between the argininosuccinate lyase of the archaeon Methanococcus maripaludis and the corresponding bacterial and eukaryal genes.

    abstract::The argH gene encoding argininosuccinate lyase (ASL) of Methanococcus maripaludis was cloned on a 4.7-kb HindIII genomic fragment. The gene is preceded by a short open reading frame (ORF149), which encodes a polypeptide with an unknown function. The two genes are co-transcribed. The ASL of M. maripaludis shares a high...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cohen-Kupiec R,Kupiec M,Sandbeck K,Leigh JA

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  • A class II hydrophobin gene, Trhfb3, participates in fungal asexual development of Trichoderma reesei.

    abstract::Hydrophobin proteins originate from filamentous fungi, which are able to self-assemble at water-air interfaces. Hydrophobins have multiple functions in fungal growth and development. In this study, the function of the Trhfb3 gene encoding a class II hydrophobin was characterized in Trichoderma reesei. The null mutant ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: He R,Li C,Feng J,Zhang D

    更新日期:2017-04-01 00:00:00

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    abstract::The genes encoding transferrin-binding proteins (TBPs) 1 and 2 of Neisseria meningitidis and N. gonorrhoeae were used as model loci in a novel method of cloning (twin N-terminal polymerase chain reaction; TNT-PCR) involving amplification between the 5' ends of two genes. Primers were based on N-terminal amino-acid seq...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wilton J,Ala'Aldeen D,Palmer HM,Borriello SP

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wanderley KJ,Torres FA,Moraes LM,Ulhoa CJ

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cateau E,Verdon J,Fernandez B,Hechard Y,Rodier MH

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  • Inhibitory effect of lipopolysaccharide on apoptotic cell death in macrophages infected with Actinobacillus actinomycetemcomitans.

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Muro M,Nakashima K,Tomioka J,Kato S,Nonaka K,Yoshida T,Inoue M,Nishihara T,Kowashi Y

    更新日期:1999-06-15 00:00:00

  • Isolation of genes encoding beta-D-xylanase, beta-D-xylosidase and alpha-L-arabinofuranosidase activities from the rumen bacterium Prevotella ruminicola B1(4).

    abstract::Prevotella ruminicola B1(4) is a strictly anaerobic, Gram-negative, polysaccharide-degrading rumen bacterium. Xylanase activity in this strain was found to be inducible, the specific activity of cells grown on xylan being increased at least 20-fold by comparison with cells grown on glucose. Ten bacteriophage clones ex...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gasparic A,Marinsek-Logar R,Martin J,Wallace RJ,Nekrep FV,Flint HJ

    更新日期:1995-01-15 00:00:00

  • Optimized conditions for the fermentation of Helicobacter pylori and production of vacuolating cytotoxin.

    abstract::We report here improvements to the growth media and fermentation conditions which result in a substantial increase of Helicobacter pylori growth and in the enhanced production of vacuolating cytotoxin. Addition of glucose to the medium resulted in the increase of cell yield, cell viability and a significant improvemen...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Marchini A,Massari P,Manetti R,Olivieri R

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  • Impact of land use history on the arbuscular mycorrhizal fungal diversity in arid soils of Argentinean farming fields.

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ontivero RE,Voyron S,Allione LVR,Bianco P,Bianciotto V,Iriarte HJ,Lugo MA,Lumini E

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    journal_title:FEMS microbiology letters

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  • vpsA- and luxO-independent biofilms of Vibrio cholerae.

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Müller J,Miller MC,Nielsen AT,Schoolnik GK,Spormann AM

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    journal_title:FEMS microbiology letters

    pub_type: 信件


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    pub_type: 杂志文章


    authors: Novak K,Pflügl S

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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