Repression of the TreR transcriptional regulator in Streptococcus mutans by the global regulator, CcpA.

Abstract:

:Streptococcus mutans, the etiologic agent of dental caries in humans, is considered a dominating force in the oral microbiome due to its highly-evolved propensity for survival. The oral pathogen encodes an elaborate array of regulatory elements, including the carbon catabolite-responsive regulator, CcpA, a global regulator key in the control of sugar metabolism and in stress tolerance response mechanisms. The recently characterized trehalose utilization operon, integral for the catabolism of the disaccharide trehalose, is controlled by a local regulator, TreR, which has been implicated in a number of cellular functions outside of trehalose catabolism. Electrophoretic mobility shift assays demonstrated that CcpA bound a putative cre site in the treR promoter. Loss of ccpA resulted in elevated expression of treR in cultures of the organism grown in glucose or trehalose, indicating that CcpA not only acts as a repressor of trehalose catabolism genes, but also the local regulator. The loss of both CcpA and TreR in S. mutans resulted in an impaired growth rate and fitness response, supporting the hypothesis that these regulators are involved in carbon catabolism control and in induction of components of the organism's stress response.

journal_name

FEMS Microbiol Lett

authors

Lindsay EL,Faustoferri RC,Quivey RG

doi

10.1093/femsle/fnab004

subject

Has Abstract

pub_date

2021-01-16 00:00:00

eissn

0378-1097

issn

1574-6968

pii

6102557

pub_type

杂志文章
  • Gloeosporiocide, a new antifungal cyclic peptide from Streptomyces morookaense AM25 isolated from the Amazon bulk soil.

    abstract::Actinobacteria are known by their ability to produce several antimicrobial compounds of biotechnological interest. Thus, in this study, we isolated and identified by partial 16S RNA sequencing ∼100 actinobacteria isolates from guarana (Paullinia cupana) bulk soil. Besides, we isolated from the actinobacteria Streptomy...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz175

    authors: Vicente Dos Reis G,Abraham WR,Grigoletto DF,de Campos JB,Marcon J,da Silva JA,Quecine MC,de Azevedo JL,Ferreira AG,de Lira SP

    更新日期:2019-07-01 00:00:00

  • DNA from Aspergillus flavus contains 5-methylcytosine.

    abstract::DNA from Aspergillus sp. has been reported not to contain 5-methylcytosine. However, it has been found that Aspergillus nidulans responds to 5-azacytidine, a drug that is a strong inhibitor of DNA methyltransferases. Therefore, we have re-examined the occurrence of 5-methylcytosine in DNA from Aspergillus flavus by us...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10939.x

    authors: Gowher H,Ehrlich KC,Jeltsch A

    更新日期:2001-11-27 00:00:00

  • Broad range DNA probes for detecting and amplifying eubacterial nucleic acids.

    abstract::In this report we describe and characterize two oligomer probes that are broadly homologous to conserved eubacterial 16S ribosomal RNA (rRNA) sequences not present in human 18 rRNA or human mitochondrial 12S rRNA. One or both of the probes can detect all of 23 phylogenetically diverse eubacterial nucleic acids against...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(89)90139-0

    authors: Chen K,Neimark H,Rumore P,Steinman CR

    更新日期:1989-01-01 00:00:00

  • Typing of toxic strains of Clostridium difficile using DNA fingerprints generated with arbitrary polymerase chain reaction primers.

    abstract::Clostridium difficile is the causative agent for pseudomembranous colitis in humans. Toxic strains of C. difficile produce two toxins, toxin A and toxin B. A reliable and definitive method of typing the toxic strains of C. difficile is needed since nosocomial cross infection is a primary concern in hospitals and other...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90532-s

    authors: McMillin DE,Muldrow LL

    更新日期:1992-04-01 00:00:00

  • Evidence that the Piromyces gene family encoding endo-1,4-mannanases arose through gene duplication.

    abstract::The sequences of two Piromyces cDNAs (manB and manC) encoding functional mannanases, defined as mannanase B (MANB) and mannanase C (MANC), revealed that both the cDNAs, and the encoded enzymes, exhibited extensive sequence identity with each other and with a previously described Piromyces mannanase. MANB and MANC, whi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08382.x

    authors: Millward-Sadler SJ,Hall J,Black GW,Hazlewood GP,Gilbert HJ

    更新日期:1996-08-01 00:00:00

  • Listeria monocytogenes infection of Caco-2 human epithelial cells induces activation of transcription factor NF-kappa B/Rel-like DNA binding activities.

    abstract::The effect of Listeria monocytogenes infection on the cellular level of transcription factor NF-kappa B in the human epithelia-like cell line Caco-2 was investigated. Infection with L. monocytogenes or treatment with lipoteichoic acid induced the formation of three NF-kappa B-like DNA-protein complexes C1, C2, and C3,...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13766.x

    authors: Hauf N,Goebel W,Fiedler F,Kuhn M

    更新日期:1999-09-01 00:00:00

  • Inactivation of the fis gene leads to reduced growth rate.

    abstract::The FIS protein (factor for inversion stimulation) is known to activate the transcription of rRNA and tRNA operons in Escherichia coli. In this report the growth behaviour of a fis bacterium was studied under steady-state conditions and in competition experiments with a wild-type bacterium in a chemostat. The growth r...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90292-v

    authors: Nilsson L,Verbeek H,Hoffmann U,Haupt M,Bosch L

    更新日期:1992-11-15 00:00:00

  • Co-cultivation of antifungal Lactobacillus plantarum MiLAB 393 and Aspergillus nidulans, evaluation of effects on fungal growth and protein expression.

    abstract::The fungal inhibitory effects of strain Lactobacillus plantarum MiLAB 393, producing broad-spectrum antifungal compounds, were evaluated. A co-cultivation method was set up to monitor effects on fungal growth and protein expression of growing Aspergillus nidulans with L. plantarum MiLAB 393. The effects of inhibitory ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.03.047

    authors: Ström K,Schnürer J,Melin P

    更新日期:2005-05-01 00:00:00

  • Comparison of the effect of salinity on the D/H ratio of fatty acids of heterotrophic and photoautotrophic microorganisms.

    abstract::The core metabolism of microorganisms has a major influence on the hydrogen isotopic composition of their fatty acids. Heterotrophic microorganisms produce fatty acids with a deuterium to hydrogen (D/H) ratio either slightly depleted or enriched in D compared to the growth water, while photo- and chemoautotrophic micr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv065

    authors: Heinzelmann SM,Chivall D,M'Boule D,Sinke-Schoen D,Villanueva L,Damsté JS,Schouten S,van der Meer MT

    更新日期:2015-05-01 00:00:00

  • Comparative analysis of Methicillin-Resistant Staphylococcus aureus (MRSA) and Borderline Oxacillin Resistant Staphylococcus aureus (BORSA) in community and food of animal origin.

    abstract::The aim of this study was to illustrate the relative pervasiveness of Borderline Oxacillin Resistant Staphylococcus aureus (BORSA) and Methicillin-Resistant Staphylococcus aureus (MRSA) in community and food of animal origin and their relationship with other genetic determinants. Staphylococcus aureus isolates were su...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnaa201

    authors: Zehra A,Gulzar M,Singh R,Kaur S,Gill JPS

    更新日期:2020-12-22 00:00:00

  • Expression of micF involved in porin synthesis in Escherichia coli: two distinct cis-acting elements respectively regulate micF expression positively and negatively.

    abstract::micF RNA, whose sequence is highly complementary to a 5'-portion of ompF mRNA, has been implicated in the osmoregulation and thermoregulation of the ompF porin gene in Escherichia coli. To define and characterize cis-acting regulatory regions upstream of the micF promoter, a series of deletions of the micF promoter fu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(91)90440-l

    authors: Takayanagi K,Maeda S,Mizuno T

    更新日期:1991-09-15 00:00:00

  • Rapid detection of tetM in Mycoplasma hominis and Ureaplasma urealyticum by PCR: tetM confers resistance to tetracycline but not necessarily to doxycycline.

    abstract::Tetracycline resistance in Mycoplasma hominis and Ureaplasma urealyticum has been associated with the tetM determinant and has recently been increasing in incidence. We report here a rapid method for detection of the tetM determinant based on the use of the polymerase chain reaction (PCR) to amplify a 397-bp DNA fragm...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90442-q

    authors: Blanchard A,Crabb DM,Dybvig K,Duffy LB,Cassell GH

    更新日期:1992-08-15 00:00:00

  • Correlation between cell-associated mannose-sensitive hemagglutination by Vibrio parahaemolyticus and adherence to a human colonic cell line Caco-2.

    abstract::Cell-associated hemagglutination (cHA) activity with human erythrocytes was examined for 468 clinical and 71 environmental strains of Vibrio parahaemolyticus. Approximately 95% of the strains tested were cHA positive irrespective of source or Kanagawa phenomenon. 75% of clinical strains showed relatively strong mannos...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb07032.x

    authors: Nagayama K,Oguchi T,Arita M,Honda T

    更新日期:1994-07-01 00:00:00

  • Identification of the sugars involved in mycobacterial cell aggregation.

    abstract::Incubation of Mycobacterium tuberculosis and M smegmatis cells with the sugar components of their surface-exposed glycans demonstrated that D-arabinose but not alpha-D-glucose or D-mannose, led to the dispersion of the large clumps formed by the bacilli in stationary liquid cultures. These results confirm the presence...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08525.x

    authors: Anton V,Rougé P,Daffé M

    更新日期:1996-11-01 00:00:00

  • High-quality mutant libraries of Xanthomonas oryzae pv. oryzae and X. campestris pv. campestris generated by an efficient transposon mutagenesis system.

    abstract::A novel transposon mutagenesis system for the phytopathogenic bacteria Xanthomonas oryzae pv. oryzae (Xoo) and X. campestris pv. campestris (Xcc) was developed using a Tn5-based transposome. A highly efficient transformation up to 10(6) transformants per microg transposon DNA was obtained. Southern blot and thermal as...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00583-4

    authors: Sun Q,Wu W,Qian W,Hu J,Fang R,He C

    更新日期:2003-09-12 00:00:00

  • Development of a gene knockout system for Ralstonia eutropha H16 based on the broad-host-range vector expressing a mobile group II intron.

    abstract::Ralstonia eutropha H16 is a Gram-negative lithoautotrophic bacterium and is one of the best biopolymer-producing bacteria. It can grow to high cell densities either under lithoautotrophic or under heterotrophic conditions, which makes it suitable for a number of biotechnological applications. Also, R. eutropha H16 can...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.02041.x

    authors: Park JM,Jang YS,Kim TY,Lee SY

    更新日期:2010-08-01 00:00:00

  • The light-harvesting complex II (B800-850) of Rhodobacter sulfidophilus: characterization and formation under different growth conditions.

    abstract::The photosynthetic bacterium Rhodobacter sulfidophilus is able to grow chemotrophically and phototrophically at a broad range of light intensities. In contrast to other facultative phototrophs, R. sulfidophilus synthesizes reaction center and light-harvesting (LH) complexes, B870 (LHI) and B800-850 (LHII) even under f...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07382.x

    authors: Hagemann GE,Gad'on N,Garcia A,Drews G,Tadros MH

    更新日期:1995-02-01 00:00:00

  • Evidence for importance of the Staphylococcus hyicus lipase pro-peptide in lipase secretion, stability and activity.

    abstract::To investigate the function of the pro-peptide (PP) region of the Staphylococcus hyicus exolipase, restriction sites were created in the lipase gene to facilitate the construction of deletions in this region. Lipase gene expression was carried out in Staphylococcus carnosus. In the presence of the entire PP region, th...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb07098.x

    authors: Demleitner G,Götz F

    更新日期:1994-08-15 00:00:00

  • Characterization of the proteins encoded by the Bacillus subtilis yoxA-dacC operon.

    abstract::In Bacillus subtilis, the yoxA and dacC genes were proposed to form an operon. The yoxA gene was overexpressed in Escherichia coli and its product fused to a polyhistidine tag was purified. An aldose-1-epimerase or mutarotase activity was measured with the YoxA protein that we propose to rename as GalM by analogy with...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01761.x

    authors: Duez C,Zervosen A,Teller N,Melkonian R,Banzubazé E,Bouillenne F,Luxen A,Frère JM

    更新日期:2009-11-01 00:00:00

  • Comparative analysis of gene expression in Streptococcus pneumoniae and Lactococcus lactis.

    abstract::The pFL10 plasmid vector for translational fusions was constructed. pFL10 is based in the promiscuous pLS1 replicon and contains the pC194 cat gene deprived of its transcriptional promoter and Shine-Dalgarno (SD) sequence. Three promoters (Pcit, PpolA and PtetL) from Gram-positive bacteria, inserted in pFL10, were tes...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb07182.x

    authors: López de Felipe F,Corrales MA,López P

    更新日期:1994-10-01 00:00:00

  • Genomic suppression subtractive hybridization as a tool to identify differences in mycorrhizal fungal genomes.

    abstract::Characterization of genomic variation among different microbial species, or different strains of the same species, is a field of significant interest with a wide range of potential applications. We have investigated the genomic variation in mycorrhizal fungal genomes through genomic suppressive subtractive hybridizati...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02248.x

    authors: Murat C,Zampieri E,Vallino M,Daghino S,Perotto S,Bonfante P

    更新日期:2011-05-01 00:00:00

  • Characterization of Vibrio vulnificus isolated from cockles (Anadara granosa): antimicrobial resistance, plasmid profiles and random amplification of polymorphic DNA analysis.

    abstract::Antibiotic susceptibility, plasmid profiles and random amplification of polymorphic DNA (RAPD) were used to study strains of Vibrio vulnificus isolated from cockles (Anadara granosa). Thirty-six isolates were analyzed. The prevalent biotypes were 1 (72.2% of the isolates) and 2 (27.8%). Among these, 21 strains of biot...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13138.x

    authors: Radu S,Elhadi N,Hassan Z,Rusul G,Lihan S,Fifadara N,Yuherman,Purwati E

    更新日期:1998-08-01 00:00:00

  • Molecular characterization of newly identified IS3, IS4 and IS30 insertion sequence-like elements in Mycoplasma bovis and their possible roles in genome plasticity.

    abstract::Insertion sequences (ISs) are mobile genetic elements widely distributed among bacteria. Their impact on the bacterial genome is multifold, including transfer of genetic information, shuttle of adaptive traits and influence on the genomic content. As a result, ISs play an important role in the organization, plasticity...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/j.1574-6968.2009.01562.x

    authors: Lysnyansky I,Calcutt MJ,Ben-Barak I,Ron Y,Levisohn S,Methé BA,Yogev D

    更新日期:2009-05-01 00:00:00

  • Escherichia coli strain Nissle 1917-from bench to bedside and back: history of a special Escherichia coli strain with probiotic properties.

    abstract::Among the gram-negative microorganisms with probiotic properties, Escherichia coli strain Nissle 1917 (briefly EcN) is probably the most intensively investigated bacterial strain today. Since nearly 100 years, the EcN strain is used as the active pharmaceutical ingredient in a licensed medicinal product that is distri...

    journal_title:FEMS microbiology letters

    pub_type: 历史文章,杂志文章,评审

    doi:10.1093/femsle/fnw212

    authors: Sonnenborn U

    更新日期:2016-10-01 00:00:00

  • Induction of a viable but non-culturable state in Salmonella Typhimurium by thermosonication and factors affecting resuscitation.

    abstract::The objective of this work was to analyze the effects of thermosonication (TS) on induction of a viable but non-culturable (VBNC) state in Salmonella Typhimurium and to examine incubation factors affecting subsequent resuscitation of cells. A TS treatment of 380 W at 53°C for 30 min induced the VBNC state in S. Typhim...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnx249

    authors: Liao H,Jiang L,Zhang R

    更新日期:2018-02-01 00:00:00

  • Rapid identification of the species of the Bacteroides fragilis group by multiplex PCR assays using group- and species-specific primers.

    abstract::We report a rapid and reliable two-step multiplex polymerase chain reaction (PCR) assay to identify the 10 Bacteroides fragilis group species - Bacteroides caccae, B. distasonis, B. eggerthii, B. fragilis, B. merdae, B. ovatus, B. stercoris, B. thetaiotaomicron, B. uniformis and B. vulgatus. These 10 species were firs...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00296-9

    authors: Liu C,Song Y,McTeague M,Vu AW,Wexler H,Finegold SM

    更新日期:2003-05-16 00:00:00

  • Molecular cloning of a novel allele of SMR1 which determines sulfometuron methyl resistance in Saccharomyces cerevisiae.

    abstract::A different mutation (SMR1B) to SMR1-410 (ilv2), which determines resistance to sulfometuron methyl in Saccharomyces cerevisiae, was cloned by PCR. Sequence analysis indicated a C to T change at position 575 of the ILV2 coding sequence, which results in a proline transition to leucine at position 192. Similarly to SMR...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08100.x

    authors: Xie Q,Jiménez A

    更新日期:1996-04-01 00:00:00

  • Eight-hour PCR-based procedure for the detection of Salmonella in raw oysters.

    abstract::The aim of this study was to evaluate the ability of a nested PCR system to detect Salmonella senftenberg in raw oysters. The specific primers of the PCR were derived from the invA gene sequence, essential for Salmonella invasiveness into epithelial cells. First, for the extraction of DNA, four methods (guanidine isot...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.12.016

    authors: Vázquez-Novelle MD,Pazos AJ,Abad M,Sánchez JL,Pérez-Parallé ML

    更新日期:2005-02-01 00:00:00

  • Stable, temperature-sensitive recombinant strain of Mycobacterium smegmatis generated through the substitution of a psychrophilic ligA gene.

    abstract::A synthetic version of the ligA gene encoding the NAD-dependent DNA ligase from the Arctic bacterium Pseudoalteromonas haloplanktis was substituted for its homolog in the chromosome of Mycobacterium smegmatis. The resulting recombinant strain grew identically to the parent strain at permissive temperatures but failed ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv152

    authors: Pinto CT,Nano FE

    更新日期:2015-09-01 00:00:00

  • Luminometry and PCR-based monitoring of gene-tagged cyanobacteria in Baltic Sea microcosms.

    abstract::The cyanobacterium Synechocystis 6803 was tagged by chromosomal integration of the firefly luciferase gene, luc, resulting in the modified strain Synechocystis 6803-luc. The tagged cells were monitored in Baltic Sea microcosms both by detection of the luc gene by PCR amplification and by measurement of luc gene expres...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(95)00133-P

    authors: Möller A,Norrby AM,Gustafsson K,Jansson J

    更新日期:1995-06-01 00:00:00