Diatom DNA barcodes for forensic discrimination of drowning incidents.

Abstract:

:The presence of diatoms in victim's internal organs has been regarded as a gold biological evidence of drowning. The idea becomes true at the advent of DNA metabarcoding. Unfortunately, the DNA barcode of diatoms are far from being applicable due to neither consensus on the barcode and nor reliable reference library.In this study we tested 23 pairs of primers, including two new primer pairs, Baci18S (V4 of 18S) and BacirbcL (central region of rbcL), for amplifying fragments of 16S/18S, 23S/28S, COI, ITS and rbcL. A total of five pairs of primers performed satisfactory for diatoms. We used three of them, 18S605 (V2 + V3 of 18S), Baci18S and BacirbcL, to barcode four water samples using next generation sequencing platform. The results showed that these primers worked well for NGS metabarcoding of diatoms. We suggest that 18S605, Baci18S and BacirbcL be barcodes of diatoms and the corresponding primer pairs be used. Considering a quite high proportion of sequences deposited in GenBank were mislabeled, the most urgent task for DNA barcoding of diatoms is to create standard sequences using correctly identified specimens, ideally type specimens.

journal_name

FEMS Microbiol Lett

authors

Liu M,Zhao Y,Sun Y,Wu P,Zhou S,Ren L

doi

10.1093/femsle/fnaa145

subject

Has Abstract

pub_date

2020-09-01 00:00:00

issue

17

eissn

0378-1097

issn

1574-6968

pii

5896452

journal_volume

367

pub_type

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