Use of a microarray to assess the distribution of plasmid and chromosomal virulence genes in strains of enteroaggregative Escherichia coli.

Abstract:

:A DNA microarray was used to analyze the distribution of plasmid and chromosomal genes among strains of enteroaggregative Escherichia coli (EAEC) isolated from a prospective diarrhoea surveillance study in the United Kingdom. Target genes were extracted from existing databases and from the genome sequence of prototype EAEC strain 042. We found that strains exhibiting the aggregative adherence (AA) phenotype could be broadly divided into two groups depending upon whether they harboured genes from the EAEC virulence plasmid (pAA) and a set of chromosomal genes found in EAEC strain 042. Several chromosomal loci were inherited en bloc, and were more common in strains which we designated Group 1; genes at the pheU locus were particularly conserved. Genes encoded on the pAA plasmid and those under control of the master regulator AggR were also concentrated in the Group 1 EAEC. A gene encoding a type 1 pilin allele was detected more frequently in Group 2 EAEC. Our data suggest that strains previously designated as typical EAEC harbour a large number of conserved plasmid and chromosomal loci, further illuminating a package of virulence genes common to the most important EAEC.

journal_name

FEMS Microbiol Lett

authors

Jenkins C,van Ijperen C,Dudley EG,Chart H,Willshaw GA,Cheasty T,Smith HR,Nataro JP

doi

10.1016/j.femsle.2005.09.040

keywords:

subject

Has Abstract

pub_date

2005-12-01 00:00:00

pages

119-24

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(05)00676-2

journal_volume

253

pub_type

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