Global transcriptional profiles of Staphylococcus aureus treated with berberine chloride.


:In this study, we show that berberine chloride (BBR) has antimicrobial activities against all 43 tested strains of Staphylococcus aureus, an important human and animal pathogen. However, the response mechanisms of S. aureus to BBR are still poorly understood. Affymetrix GeneChips were used to determine the global transcription of S. aureus triggered by treatment with subinhibitory concentrations of BBR. 468 genes were up-regulated and 262 genes were down-regulated upon exposure to BBR. There was elevated transcription of various transporter genes, including genes involved in multidrug resistance, members of the multidrug and toxin extrusion family, the ferrous iron transporter, the amino acid transporter, the Na(+)/H(+) antiporter, and the potassium cation transporter. Measurements of active transport were used to demonstrate a phenotypic correlation between efflux transporter overexpression and inhibition of BBR uptake. Furthermore, BBR induced the expression of urease genes, sortase enzyme, and iron-regulated surface determinant genes, but repressed transcription of a gene encoding arylamine N-acetyltransferase activity (N315-SA2490). To our knowledge, this is the first analysis of a genome-wide transcription profile of S. aureus cells in response to BBR treatment. These results will pave the way to exploring the mechanisms of BBR against S. aureus.


FEMS Microbiol Lett


Wang D,Yu L,Xiang H,Fan J,He L,Guo N,Feng H,Deng X




Has Abstract


2008-02-01 00:00:00














  • Enzymatic versus spontaneous S-methyl thioester synthesis in Geotrichum candidum.

    abstract::The synthesis of short chain S-methyl thioesters was investigated in Geotrichum candidum strain GcG. The results indicated the involvement of an enzymatic reaction in this microorganism that led to the synthesis of S-methyl thioacetate (MTA) when methanethiol and acetyl-CoA were used as substrates. MTA was generated f...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Helinck S,Spinnler HE,Parayre S,Dame-Cahagne M,Bonnarme P

    更新日期:2000-12-15 00:00:00

  • The influence of sigma factors and ribosomal recognition elements on heterologous expression of cyanobacterial gene clusters in Escherichia coli.

    abstract::Cyanobacterial natural products offer new possibilities for drugs and lead compounds but many factors can inhibit the production of sufficient yields for pharmaceutical processes. While Escherichia coli and Streptomyces sp. have been used as heterologous expression hosts to produce cyanobacterial natural products, the...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wells KN,Videau P,Nelson D,Eiting JE,Philmus B

    更新日期:2018-08-01 00:00:00

  • Biochemical characterization of isovaleryl-CoA dehydrogenase (LiuA) of Pseudomonas aeruginosa and the importance of liu genes fora functional catabolic pathway of methyl-branched compounds.

    abstract::Growth of Pseudomonas aeruginosa on acyclic terpene alcohols (citronellol) and on other methyl-branched compounds such as leucine or isovalerate requires a functional leucine/isovalerate utilization (Liu) pathway. In this study, we investigated the liuABCDE gene cluster by insertion mutant analysis, heterologous expre...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Förster-Fromme K,Jendrossek D

    更新日期:2008-09-01 00:00:00

  • Regulatory role of pyruvate-sensing BtsSR in biofilm formation by Escherichia coli K-12.

    abstract::Pyruvate, the key regulator in connection of a variety of metabolic pathways, influences transcription of the Escherichia coli genome through controlling the activity of two pyruvate-sensing two-component systems (TCSs), BtsSR and PyrSR. Previously, we identified the whole set of regulatory targets of PyrSR with low-a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ogasawara H,Ishizuka T,Yamaji K,Kato Y,Shimada T,Ishihama A

    更新日期:2019-12-01 00:00:00

  • A surface polysaccharide forms when gonococci are converted to serum resistance by cytidine 5'-monophospho-N-acetyl neuraminic acid.

    abstract::A serum-susceptible, guinea-pig chamber-passaged, laboratory strain (BS4 (agar)) of Neisseria gonorrhoeae was converted to serum resistance by incubation with cytidine 5-monophospho-N-acetyl neuraminic acid (CMP-NANA) and examined by electron microscopy after staining with ruthenium-red. In contrast to serum susceptib...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fox AJ,Curry A,Rowland PL,Lancaster S,Jones DM,Parsons NJ,Cole JA,Smith H

    更新日期:1990-01-01 00:00:00

  • Beyond the single answer: a process-oriented exam for science students.

    abstract::This commentary describes an assessment exercise known as the TRIPSE (Tri-Partite Problem Solving Exercise) that mimics science in operation. Students frame hypotheses based on limited data, design experiments to test them, which they later revise with new information. It is emphasised that there are no single correct...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Nastos S,Rangachari PK

    更新日期:2016-08-01 00:00:00

  • Cloning and characterization of a novel Cry1A toxin from Bacillus thuringiensis with high toxicity to the Asian corn borer and other lepidopteran insects.

    abstract::A novel cry1A was cloned from Bacillus thuringiensis strain BT8 and expressed in the B. thuringiensis acrystalliferous mutant HD73(-). The gene, designated cry1Ah1, encoded a protein with a molecular weight of 134 kDa. Reverse transcriptase-PCR and Western blotting showed that Cry1Ah was expressed in the host strain B...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Xue J,Liang G,Crickmore N,Li H,He K,Song F,Feng X,Huang D,Zhang J

    更新日期:2008-03-01 00:00:00

  • Characterization of a periplasmic peptidyl-prolyl cis-trans isomerase in Erwinia chrysanthemi.

    abstract::The main determinant of the plant pathogen Erwinia chrysanthemi virulence is the production of extracellular enzymes, mainly pectate lyases. Adjacent to a pectate lyase encoding locus, we identified the gene rotA supposed to encode a folding catalyst. Overproduction of the protein and assay of activity using a synthet...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Pissavin C,Hugouvieux-Cotte-Pattat N

    更新日期:1997-12-01 00:00:00

  • Loop-mediated isothermal amplification for the rapid detection of Salmonella.

    abstract::Loop-mediated isothermal amplification (LAMP) assay detected Salmonella within 60 min. The 220 strains of 39 serotypes of Salmonella subsp. enterica and 7 strains of Salmonella enterica subsp. arizonae were amplified, but not 62 strains of 23 bacterial species other than Salmonella. The sensitivity of the LAMP assay w...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hara-Kudo Y,Yoshino M,Kojima T,Ikedo M

    更新日期:2005-12-01 00:00:00

  • The Listeria monocytogenes prfAP2 promoter is regulated by sigma B in a growth phase dependent manner.

    abstract::Listeria monocytogenes prfA, encoding positive regulatory factor A, is transcribed from three promoters (prfAP1, prfAP2, and PplcA). The prfAP2 promoter was previously proposed to be sigma B (sigma(B))-dependent. This hypothesis was tested by creating prfA promoter-gus transcriptional fusions in both L. monocytogenes ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Schwab U,Bowen B,Nadon C,Wiedmann M,Boor KJ

    更新日期:2005-04-15 00:00:00

  • The phylogeny of Aerococcus and Pediococcus as determined by 16S rRNA sequence analysis: description of Tetragenococcus gen. nov.

    abstract::The phylogenetic interrelationships of the genera Pediococcus and Aerococcus were investigated using reverse transcriptase sequencing of 16S rRNA. The genus Pediococcus was found to be phylogenetically heterogeneous. The four species P. acidilactici, P. damnosus, P. parvulus and P. pentosaceus formed a phylogeneticall...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Collins MD,Williams AM,Wallbanks S

    更新日期:1990-08-01 00:00:00

  • Cloning and analysis of the polyhydroxyalkanoic acid synthase gene from an Acinetobacter sp.: evidence that the gene is both plasmid and chromosomally located.

    abstract::The polyhydroxyalkanoic acid (PHA) synthase gene (phaCAc) of a species of Acinetobacter isolated from an activated sludge treatment plant was cloned by heterologous complementation in a poly-beta-hydroxybutyrate (PHB) negative mutant of Alcaligenes eutrophus. Nucleotide sequence analysis of phaCAc revealed an open rea...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Schembri MA,Bayly RC,Davies JK

    更新日期:1994-05-01 00:00:00

  • Characterization of an F18+ enterotoxigenic Escherichia coli strain from post weaning diarrhoea of swine, and of its conjugative virulence plasmid pTC.

    abstract::The enterotoxigenic Escherichia coli (ETEC) strain Ec2173, causing post weaning diarrhoea in swine, harbours six plasmids ranging from 13 to 200 kb in size. The heat stable toxin genes sta, stb and a tetracycline resistance gene were located on a self conjugative 120-kb plasmid, called pTC. In the cloned ColE1 type or...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Olasz F,Fekete PZ,Blum-Oehler G,Boldogkoi Z,Nagy B

    更新日期:2005-03-15 00:00:00

  • Iron repressibility of siderophore and transferrin-binding protein in Staphylococcus aureus.

    abstract::In order to investigate whether the iron acquisition mechanisms of Staphylococcus aureus are induced by iron restriction in vitro, we examined S. aureus ATCC 6538 for production of siderophore and expression of transferrin-binding protein (SA-tbp) in normal or deferrated brain heart infusion broth (BHI). Siderophore p...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lim Y,Shin SH,Lee SI,Kim IS,Rhee JH

    更新日期:1998-06-01 00:00:00

  • XaxAB-like binary toxin from Photorhabdus luminescens exhibits both insecticidal activity and cytotoxicity.

    abstract::The enterobacterium Photorhabdus luminescens produces a number of toxins to kill its insect host. By analyzing the genomic sequence of P. luminescens TT01, we found that amino acid sequences encoded by plu1961 and plu1962 showed high similarity to XaxAB binary toxin of Xenorhabuds nematophila, which has both necrotic ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Zhang X,Hu X,Li Y,Ding X,Yang Q,Sun Y,Yu Z,Xia L,Hu S

    更新日期:2014-01-01 00:00:00

  • Kinetics of electron transfer between plastocyanin and the soluble CuA domain of cyanobacterial cytochrome c oxidase.

    abstract::It has been shown that efficient functioning of photosynthesis and respiration in the cyanobacterium Synechocystis PCC 6803 requires the presence of either cytochrome c6 or plastocyanin. In order to check whether the blue copper protein plastocyanin can act as electron donor to cytochrome c oxidase, we investigated th...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Paumann M,Bernroitner M,Lubura B,Peer M,Jakopitsch C,Furtmüller PG,Peschek GA,Obinger C

    更新日期:2004-10-15 00:00:00

  • Ultra-violet resonance Raman spectroscopy for the rapid discrimination of urinary tract infection bacteria.

    abstract::The ability to identify pathogenic organisms rapidly provides significant benefits to clinicians; in particular, with respect to best prescription practices and tracking of recurrent infections. Conventional bioassays require 3-5 days before identification of an organism can be made, thus compromising the effectivenes...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jarvis RM,Goodacre R

    更新日期:2004-03-19 00:00:00

  • Comparison of SEM and VPSEM imaging techniques with respect to Streptococcus mutans biofilm topography.

    abstract::The study compared images of mature Streptococcus mutans biofilms captured at increasing magnification to determine which microscopy method is most acceptable for imaging the biofilm topography and the extracellular polymeric substance (EPS). In vitro S. mutans biofilms were imaged using (1) scanning electron microsco...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Weber K,Delben J,Bromage TG,Duarte S

    更新日期:2014-01-01 00:00:00

  • Characterization of a replication locus and formation of a higher-order complex between RepA protein and two inverted repeats in Streptomyces plasmid pSV1.

    abstract::We identified the minimal locus of 163-kb plasmid pSV1 of Streptomyces violaceoruber for the replication in S. lividans. This locus comprised a repA gene and an upstream 407-bp sequence containing two inverted repeats (IR-III and IR-IV) within an iteron, an AT-rich region and a 300-bp noncoding sequence (NCS). RepA pr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Chen Y,Tan H,Qin Z

    更新日期:2013-12-01 00:00:00

  • Analysis of Frankia evolutionary radiation using glnII sequences.

    abstract::Using a glnII (encoding glutamine synthetase II) PCR selective screening, a Frankia ACN14a gene library clone was isolated. A derived glnII-hybridising 2.7-kb HindIII subclone was characterised. Identities of 95% and 93% were observed, respectively, with the corresponding Frankia CpI1 glnI and glnII regions. A variabl...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cournoyer B,Lavire C

    更新日期:1999-08-01 00:00:00

  • Construction of the first shuttle vectors for gene cloning and homologous recombination in Mycoplasma agalactiae.

    abstract::Mycoplasma agalactiae is a worldwide ruminant pathogen that causes significant economic losses by inflicting contagious agalactia in sheep and goats. The development of efficient control strategies requires a better understanding of the mycoplasma factors that promote successful infection. However, lack of genetic too...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Chopra-Dewasthaly R,Marenda M,Rosengarten R,Jechlinger W,Citti C

    更新日期:2005-12-01 00:00:00

  • Sequence-specific endonucleases from the cyanobacterium Nostoc sp. ATCC 29132.

    abstract::The nitrogen-fixing cyanobacterium Nostoc sp. ATCC 29132 was shown to contain two sequence-specific endonucleases. Nsp(29132) I was an isoschizomer of AsuII, and Nsp(29132) II was an isoschizomer of BamHI. Nsp(29132) II was shown to generate ends that could be ligated to those generated by BamHI. ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Muro-Pastor AM,Herrero A,Flores E

    更新日期:1991-01-01 00:00:00

  • Identification of the transcription factor responsible for L-malate-dependent regulation in the marine Antarctic bacterium Pseudoalteromonas haloplanktis TAC125.

    abstract::Two-component systems are widespread in nature and constitute the most common mechanism of transmembrane signal transduction in bacteria. Recently, a functionally active two-component system consisting of malS and malR genes possibly involved in the expression of a C4-dicarboxylate transporter system (dctAB operon) wa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Papa R,Rippa V,Duilio A

    更新日期:2009-06-01 00:00:00

  • A xylanase produced by the rumen anaerobic protozoan Polyplastron multivesiculatum shows close sequence similarity to family 11 xylanases from gram-positive bacteria.

    abstract::We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Devillard E,Newbold CJ,Scott KP,Forano E,Wallace RJ,Jouany JP,Flint HJ

    更新日期:1999-12-01 00:00:00

  • Simplified characterization through site-specific protease-mediated release of affinity proteins selected by staphylococcal display.

    abstract::The production of candidate affinity proteins in a soluble form, for downstream characterization, is often a time-consuming step in combinatorial protein engineering methods. Here, a novel approach for efficient production of candidate clones is described based on direct cleavage of the affinity protein from the surfa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kronqvist N,Löfblom J,Severa D,Ståhl S,Wernérus H

    更新日期:2008-01-01 00:00:00

  • The Tat protein transport system: intriguing questions and conundrums.

    abstract::The Tat machinery catalyzes the transport of folded proteins across the cytoplasmic membrane in bacteria and the thylakoid membrane in plants. Transport occurs only in the presence of an electric field (Δψ) and/or a pH (ΔpH) gradient, and thus, Tat transport is considered to be dependent on the proton motive force (pm...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Hamsanathan S,Musser SM

    更新日期:2018-06-01 00:00:00

  • Phage vB_BsuP-Goe1: the smallest identified lytic phage of Bacillus subtilis.

    abstract::Currently, interest in phage science is on the rise again as this subject remains largely unexplored, and its potential diversity mainly untapped. Here, we present phage vB_BsuP-Goe1, a new isolate that infects Bacillus subtilis The phage forms round plaques with a matt outline on agar plates containing a B. subtilis ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Willms IM,Hertel R

    更新日期:2016-10-01 00:00:00

  • Characterization of Xanthomonas oryzae pv. oryzae recX, a gene that is required for high-level expression of recA.

    abstract::Analysis of the nucleotide sequence downstream from the Xanthomonas oryzae pv. oryzae recA gene reveals two orfs designated orfX and recX. The former has the potential to code for a 5.6 kDa protein of unknown function while the latter encodes for a putative 14.6 kDa protein with homology to RecX from various bacteria....

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sukchawalit R,Vattanaviboon P,Utamapongchai S,Vaughn G,Mongkolsuk S

    更新日期:2001-11-27 00:00:00

  • Acetaldehyde dehydrogenase activity of the AdhE protein of Escherichia coli is inhibited by intermediates in ubiquinone synthesis.

    abstract::Defects in the acd gene (which may be allelic to ubiH) result in the inactivation of the coenzyme A-linked acetaldehyde dehydrogenase activity of the multifunctional AdhE protein of Escherichia coli. This activity is restored by addition of ubiquinone-0 to cell extracts. However, the alcohol dehydrogenase activity of ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gupta S,Mat-Jan F,Latifi M,Clark DP

    更新日期:2000-01-01 00:00:00

  • Acetylation reduces the ability of CheY to undergo autophosphorylation.

    abstract::CheY, the response regulator of the chemotaxis system in Escherichia coli, can be regulated by two covalent modifications - phosphorylation and acetylation. Both covalent modifications are involved in chemotaxis, but the mechanism and role of the acetylation are still obscure. While acetylation was shown to repress th...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Li R,Chen P,Gu J,Deng JY

    更新日期:2013-10-01 00:00:00