Global transcriptional profiles of Staphylococcus aureus treated with berberine chloride.

Abstract:

:In this study, we show that berberine chloride (BBR) has antimicrobial activities against all 43 tested strains of Staphylococcus aureus, an important human and animal pathogen. However, the response mechanisms of S. aureus to BBR are still poorly understood. Affymetrix GeneChips were used to determine the global transcription of S. aureus triggered by treatment with subinhibitory concentrations of BBR. 468 genes were up-regulated and 262 genes were down-regulated upon exposure to BBR. There was elevated transcription of various transporter genes, including genes involved in multidrug resistance, members of the multidrug and toxin extrusion family, the ferrous iron transporter, the amino acid transporter, the Na(+)/H(+) antiporter, and the potassium cation transporter. Measurements of active transport were used to demonstrate a phenotypic correlation between efflux transporter overexpression and inhibition of BBR uptake. Furthermore, BBR induced the expression of urease genes, sortase enzyme, and iron-regulated surface determinant genes, but repressed transcription of a gene encoding arylamine N-acetyltransferase activity (N315-SA2490). To our knowledge, this is the first analysis of a genome-wide transcription profile of S. aureus cells in response to BBR treatment. These results will pave the way to exploring the mechanisms of BBR against S. aureus.

journal_name

FEMS Microbiol Lett

authors

Wang D,Yu L,Xiang H,Fan J,He L,Guo N,Feng H,Deng X

doi

10.1111/j.1574-6968.2007.01031.x

subject

Has Abstract

pub_date

2008-02-01 00:00:00

pages

217-25

issue

2

eissn

0378-1097

issn

1574-6968

pii

FML1031

journal_volume

279

pub_type

杂志文章
  • Production of acylated homoserine lactone by gram-positive bacteria isolated from marine water.

    abstract::Acylated homoserine lactone (AHL)-based quorum sensing (QS) has been reported to be present only in Gram-negative microorganisms. Isolation of a novel Gram-positive microorganism from sea water, capable of producing AHL, is reported here. The isolate (GenBank: JF915892, designated as MPO) belonging to the Exiguobacter...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12123

    authors: Biswa P,Doble M

    更新日期:2013-06-01 00:00:00

  • Regulation of the yeast vacuolar aminopeptidase Y gene (APY1) expression.

    abstract::The steady-state levels of the aminopeptidase Y (APY1) transcript and the level of assayable aminopeptidase Y activity were measured under a variety of nutritional conditions. Specific APY1 mRNA is less abundant in cells grown in minimal medium than in cells grown in rich medium, while active enzyme levels follow just...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08191.x

    authors: Herrera-Camacho I,Suárez-Rendueles P

    更新日期:1996-06-01 00:00:00

  • A class II hydrophobin gene, Trhfb3, participates in fungal asexual development of Trichoderma reesei.

    abstract::Hydrophobin proteins originate from filamentous fungi, which are able to self-assemble at water-air interfaces. Hydrophobins have multiple functions in fungal growth and development. In this study, the function of the Trhfb3 gene encoding a class II hydrophobin was characterized in Trichoderma reesei. The null mutant ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnw297

    authors: He R,Li C,Feng J,Zhang D

    更新日期:2017-04-01 00:00:00

  • Occurrence of aminoglycoside-modifying enzymes among isolates of Escherichia coli exhibiting high levels of aminoglycoside resistance isolated from Korean cattle farms.

    abstract::This study investigated 247 Escherichia coli isolates collected from four cattle farms to characterize aminoglycoside-modifying enzyme (AME) genes, their plasmid replicons and transferability. Out of 247 isolates a high number of isolates (total 202; 81.78%) were found to be resistant to various antibiotics by disc di...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnx129

    authors: Belaynehe KM,Shin SW,Hong-Tae P,Yoo HS

    更新日期:2017-08-01 00:00:00

  • MetJ-mediated regulation of the Salmonella typhimurium metE and metR genes occurs through a common operator region.

    abstract::In Salmonella typhimurium the metE and metR promoters overlap and are divergently transcribed. Three tandem repeats of an 8 bp sequence defined previously as the metE operator site for MetJ-mediated repression also overlap the -35 region of the metR promoter. Starting with a metE-lacZ.metR-galK double gene fusion, sit...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06090.x

    authors: Wu WF,Urbanowski ML,Stauffer GV

    更新日期:1993-04-01 00:00:00

  • The effect of buthionine sulfoximine on the growth of Leishmania donovani in culture.

    abstract::Changes in composition of the principal low molecular mass thiols of Leishmania donovani were monitored during the transformation of promastigotes, first to stationary phase metacyclic forms and then to amastigotes. No consistent variation in the thiol composition of the parasite which could account for the known incr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13495.x

    authors: Weldrick DP,Chodacka B,Vogt R,Steenkamp DJ

    更新日期:1999-04-01 00:00:00

  • Molecular characterization of the erythromycin resistance plasmid pPV142 from Staphylococcus simulans.

    abstract::The 2.5-kb erythromycin resistance (EmR) plasmid pPV142 of Staphylococcus simulans 13044 was isolated and characterized. Sequence analysis identified ORF1 and ORF2 encoding a 158-residue replication protein (Rep142) and a 244-residue erythromycin resistance protein (Erm, rRNA adenine N-6-methyltransferase), respective...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13158.x

    authors: Somkuti GA,Solaiman DK,Steinberg DH

    更新日期:1998-08-15 00:00:00

  • Bacillus polymyxa stimulates increased Rhizobium etli populations and nodulation when co-resident in the rhizosphere of Phaseolus vulgaris.

    abstract::Microbial competition for carbon sources is a primary determinant of rhizosphere ecology. We employed the PCR to examine the population fluctuations of a symbiotic nitrogen-fixing bacterium (Rhizobium etli) during the first 11 days following inoculation of Phaseolus vulgaris seedlings grown in the presence or absence ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08442.x

    authors: Petersen DJ,Srinivasan M,Chanway CP

    更新日期:1996-09-01 00:00:00

  • Variant toxin B and a functional toxin A produced by Clostridium difficile C34.

    abstract::A particular property of Clostridium difficile strain C34 is an insertion of approximately 2 kb in the tcdA-C34 gene that does not hinder expression of a fully active TcdA-C34 molecule. Intoxication with TcdA-C34 induced an arborized appearance in eukaryotic cells (D-type cytopathic effect); intoxication with TcdB-C34...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10638.x

    authors: Mehlig M,Moos M,Braun V,Kalt B,Mahony DE,von Eichel-Streiber C

    更新日期:2001-05-01 00:00:00

  • Use of a lux reporter system for monitoring rapid changes in alpha-toxin gene expression in Clostridium perfringens during growth.

    abstract::To determine whether the luxA-luxB reporter system is suitable as a sensitive reporter for rapid real-time measurements of alpha-toxin gene (cpa) expression in Clostridium perfringens, and to widen the range of alpha-toxin-producing C. perfringens strains examined with respect to cpa expression during growth, the repo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09164.x

    authors: Phillips-Jones MK

    更新日期:2000-07-01 00:00:00

  • Cloning vectors for Streptococcus thermophilus derived from a native plasmid.

    abstract::A 3.5-kb native plasmid (pND103) was identified in Streptococcus thermophilus ST2-1. Preliminary sequence analysis indicated that pND103 belongs to group I S. thermophilus plasmids. A region of approximately 2 kb appears to contain three components: a plus origin of replication (ori) typical of plasmids that replicate...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11412.x

    authors: Su P,Jury K,Allison GE,Wong WY,Kim WS,Liu CQ,Vancov T,Dunn NW

    更新日期:2002-10-29 00:00:00

  • Induced expression of the Serratia entomophila Sep proteins shows activity towards the larvae of the New Zealand grass grub Costelytra zealandica.

    abstract::Serratia entomophila and Serratia proteamaculans cause amber disease of the grass grub Costelytra zealandica (Coleoptera: Scarabaeidae). Three genes required for virulence, sepABC, are located on a large plasmid, pADAP. The translated products of the sep genes are members of the toxin complex (Tc) family of insecticid...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00886.x

    authors: Hurst MR,Jones SM,Tan B,Jackson TA

    更新日期:2007-10-01 00:00:00

  • The Aspergillus niger acuA and acuB genes correspond to the facA and facB genes in Aspergillus nidulans.

    abstract::Mutants in Aspergillus niger unable to grow on acetate as a sole carbon source were previously isolated by resistance to 1.2% propionate medium containing 0.1% glucose. AcuA mutants lacked acetyl-CoA synthetase (ACS) activity and acuB mutants lacked both ACS and isocitrate lyase activity. An acuA mutant was transforme...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13756.x

    authors: Papadopoulou S,Sealy-Lewis HM

    更新日期:1999-09-01 00:00:00

  • Molybdate-dependent expression of dimethylsulfoxide reductase in Rhodobacter capsulatus.

    abstract::Expression of the dimethylsulfoxide respiratory (dor) operon of Rhodobacter is regulated by oxygen, light intensity and availability of substrate. Since dimethylsulfoxide reductase contains a pterin molybdenum cofactor, the role of molybdate in the regulation of dor operon expression was investigated. In this report w...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09287.x

    authors: Solomon PS,Shaw AL,Young MD,Leimkuhler S,Hanson GR,Klipp W,McEwan AG

    更新日期:2000-09-15 00:00:00

  • Purification and characterization of an exo-polygalacturonase from the tomato vascular wilt pathogen Fusarium oxysporum f.sp. lycopersici.

    abstract::An exo-polygalacturonase (EC 3.2.1.15) was purified to apparent homogeneity from cultures of Fusarium oxysporum f.sp. lycopersici on synthetic medium supplemented with citrus pectin, using preparative isoelectric focusing. The enzyme, denominated PG2, had an apparent M(r) of 74000 Da upon SDS-PAGE. The pI of the main ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08592.x

    authors: Di Pietro A,Roncero MI

    更新日期:1996-12-01 00:00:00

  • Gel electrophoretic analysis of penicillin-binding proteins of coagulase negative staphylococci.

    abstract::We analyzed gel electrophoretic banding patterns of penicillin-binding proteins (PBPs) of 16 type strains of coagulase-negative staphylococci (CNS). S. epidermidis, S. haemolyticus, S. saprophyticus, S. hominis, S. xylosus, S. simulans, S. warneri, S. capitis, S. saccharolyticus, S. auricularis, S. caseolyticus, S. ga...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90343-o

    authors: Kanda K,Hiramatsu K,Yokota T

    更新日期:1990-10-01 00:00:00

  • Kluyveromyces wickerhamii killer toxin: purification and activity towards Brettanomyces/Dekkera yeasts in grape must.

    abstract::Brettanomyces/Dekkera yeasts have been identified as part of the grape yeast flora. They are well known for colonizing the cellar environmental and spoiling wines, causing haze, turbidity and strong off-flavours in wines and enhancing the volatile acidity. As the general practices applied to combat Brettanomyces/Dekke...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.02194.x

    authors: Comitini F,Ciani M

    更新日期:2011-03-01 00:00:00

  • Photorhabdus luminescens LN2 requires rpoS for nematicidal activity and nematode development.

    abstract::Photorhabdus (Enterobacteriaceae) bacteria are pathogenic to insects and mutualistic with entomopathogenic Heterorhabditis nematodes. Photorhabdus luminescens subsp. akhurstii LN2, associated with Heterorhabditis indica LN2, shows nematicidal activity against H. bacteriophora H06 infective juveniles (IJs). In the pres...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnw035

    authors: Qiu X,Wu C,Cao L,Ehlers RU,Han R

    更新日期:2016-03-01 00:00:00

  • Importance of fumarate and nitrate reduction regulatory protein for intestinal proliferation of Vibrio vulnificus.

    abstract::The sepsis caused by Vibrio vulnificus is characterized by an average incubation period of 26 h and a high mortality rate exceeding 50%. The fast growth and dissemination of V. vulnificus in vivo lead to poor clinical outcomes in patients. Therefore, elucidation of the proliferation mechanisms of this organism in vivo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnw274

    authors: Kado T,Kashimoto T,Yamazaki K,Ueno S

    更新日期:2017-01-01 00:00:00

  • Evidence that OmcB and OmpB of Geobacter sulfurreducens are outer membrane surface proteins.

    abstract::The c-type cytochrome (OmcB) and the multicopper protein (OmpB) required for Fe(III) oxide reduction by Geobacter sulfurreducens were predicted previously to be outer membrane proteins, but it is not clear whether they are positioned in a manner that permits the interaction with Fe(III). Treatment of whole cells with ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00915.x

    authors: Qian X,Reguera G,Mester T,Lovley DR

    更新日期:2007-12-01 00:00:00

  • Functional characterisation of the chaperones DnaK, DnaJ, and GrpE from Clostridium acetobutylicum.

    abstract::The DnaK chaperone system is involved in various cellular processes such as the control of the folded and oligomeric state of proteins under stress and non-stress conditions. In this study we functionally characterised the homologues of the DnaK system from Clostridium acetobutylicum DnaK, DnaJ, GrpE and OrfA were het...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13363.x

    authors: Rüngeling E,Laufen T,Bahl H

    更新日期:1999-01-01 00:00:00

  • Lactobacillus fermentum 3872 genome sequencing reveals plasmid and chromosomal genes potentially involved in a probiotic activity.

    abstract::In this report we describe a Lactobacillus fermentum 3872 plasmid (pLF3872) not previously found in any other strain of this species. The analysis of the complete sequence of this plasmid revealed the presence of a gene encoding a large collagen-binding protein (CBP), as well as the genes responsible for plasmid maint...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv068

    authors: Lehri B,Seddon AM,Karlyshev AV

    更新日期:2015-06-01 00:00:00

  • Molecular characterization of beta-lactamase genes blaA and blaB of Yersinia enterocolitica biovar 1A.

    abstract::The beta-lactamase genes blaA and blaB were detected by PCR amplification in strains of Yersinia enterocolitica biovar 1A isolated from India, Germany, France and the USA. Both genes were detected in all strains. Polymerase chain reaction-restriction fragment length polymorphism revealed genetic heterogeneity in blaA ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00191.x

    authors: Sharma S,Mittal S,Mallik S,Virdi JS

    更新日期:2006-04-01 00:00:00

  • DNA probes for identification of the ectomycorrhizal fungus Tuber magnatum Pico.

    abstract::The random amplified polymorphic DNA (RAPD) technique was used to develop DNA probes for the identification of ectomycorrhizal fungi belonging to the genus Tuber. RAPD fingerprinting revealed a high degree of interspecific variability and a low degree of intraspecific variability. One band (approximately 1.5 kb), cons...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06581.x

    authors: Lanfranco L,Wyss P,Marzachí C,Bonfante P

    更新日期:1993-12-15 00:00:00

  • DNA from Aspergillus flavus contains 5-methylcytosine.

    abstract::DNA from Aspergillus sp. has been reported not to contain 5-methylcytosine. However, it has been found that Aspergillus nidulans responds to 5-azacytidine, a drug that is a strong inhibitor of DNA methyltransferases. Therefore, we have re-examined the occurrence of 5-methylcytosine in DNA from Aspergillus flavus by us...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10939.x

    authors: Gowher H,Ehrlich KC,Jeltsch A

    更新日期:2001-11-27 00:00:00

  • Emergence of a laccase-positive variant of Azospirillum lipoferum occurs via a two-step phenotypic switching process.

    abstract::Two variants have been isolated from the wild-type Azospirillum lipoferum strain 4B. The first variant, 4V(I), spontaneously emerged from the wild-type at frequencies in the order of 10(-4) to 10(-3) per cell generation. Compared to the wild-type, the 4V(I) variant gained (production of a carotenoid-like pigment, assi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13592.x

    authors: Alexandre G,Bally R

    更新日期:1999-05-15 00:00:00

  • ATP/ADP alteration as a sign of the oxidative stress development in Escherichia coli cells under antibiotic treatment.

    abstract::The extensively discussed idea of oxidative stress development under antibiotic treatment was confirmed using an antioxidant gene expression (soxRS-, oxyR-regulon) approach, including microaerobic cultivation conditions. The killing action of antibiotics and their ability to cause peroxide oxidative stress in Escheric...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12405

    authors: Akhova AV,Tkachenko AG

    更新日期:2014-04-01 00:00:00

  • Adherence of Candida albicans to components of the subendothelial extracellular matrix.

    abstract::Candida albicans yeasts adhered avidly to extracellular matrix (ECM) proteins, type IV collagen, laminin, and fibronectin immobilized on plastic. Type IV collagen showed an increase of adherence of 400% above control values; laminin, 300%; and fibronectin, 150%. In addition, all three (in quantities of 0.02-200 microg...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/s0378-1097(05)80049-7

    authors: Klotz SA

    更新日期:1990-03-15 00:00:00

  • Structure of the Bordetella pertussis gene coding for the serotype 3 fimbrial subunit.

    abstract::Bordetella pertussis strains contain at least three distinct genes coding for fimbrial subunits, designated fim2, fim3, and fimX. The sequences of the fim2 and fimX genes have been published. Here we present the sequence of the fim3 gene. Proximal and distal to the fim3 gene, regions were observed that could function ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90307-c

    authors: Mooi FR,ter Avest A,van der Heide HG

    更新日期:1990-01-01 00:00:00

  • Tetratricopeptide repeats are essential for PcrH chaperone function in Pseudomonas aeruginosa type III secretion.

    abstract::The type III secretion system (T3SS) is a specialized apparatus evolved by Gram-negative bacteria to deliver effector proteins into host cells, thus facilitating the establishment of an infection. Effector translocation across the target cell plasma membrane is believed to occur via pores formed by at least two secret...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2005.00099.x

    authors: Bröms JE,Edqvist PJ,Forsberg A,Francis MS

    更新日期:2006-03-01 00:00:00