Abstract:
:Yeast two-hybrid and Far Western analyses were used to detect interactions between Bacillus subtilis spores' nutrient germinant receptor proteins and proteins encoded by the spoVA operon, all of which are involved in spore germination and located in the spores' inner membrane. These analyses indicated that two subunits of the GerA nutrient germinant receptor interact, consistent with previous genetic data, and that some GerA proteins interact with SpoVAD and some with SpoVAE. SpoVA proteins appear to be involved in the release of the spore's dipicolinic acid during spore germination, an event triggered by the binding of nutrient germinants to their receptors. Consequently, these new findings suggest that nutrient germinant receptors physically contact SpoVA proteins, and presumably this is a route for signal transduction during spore germination.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Vepachedu VR,Setlow Pdoi
10.1111/j.1574-6968.2007.00807.xsubject
Has Abstractpub_date
2007-09-01 00:00:00pages
42-7issue
1eissn
0378-1097issn
1574-6968pii
FML807journal_volume
274pub_type
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