Abstract：
:The Tat machinery catalyzes the transport of folded proteins across the cytoplasmic membrane in bacteria and the thylakoid membrane in plants. Transport occurs only in the presence of an electric field (Δψ) and/or a pH (ΔpH) gradient, and thus, Tat transport is considered to be dependent on the proton motive force (pmf). This presents a fundamental and major challenge, namely, that the Tat system catalyzes the movement of large folded protein cargos across a membrane without collapse of ion gradients. Current models argue that the active translocon assembles de novo for each cargo transported, thus providing an effective gating mechanism to minimize ion leakage. A limited structural understanding of the intermediates occurring during transport and the role of the pmf in stabilizing and/or driving this process have hindered the development of more detailed models. A fundamental question that remains unanswered is whether the pmf is actually 'consumed', providing an energetic driving force for transport, or alternatively, whether its presence is instead necessary to provide the appropriate environment for the translocon components to become active. Including addressing this issue in greater detail, we explore a series of additional questions that challenge current models, and, hopefully, motivate future work.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Hamsanathan S,Musser SMdoi
10.1093/femsle/fny123subject
Has Abstractpub_date
2018-06-01 00:00:00issue
12eissn
0378-1097issn
1574-6968pii
5000164journal_volume
365pub_type
杂志文章,评审abstract：:Metabolic engineering is the practice of using directed genetic manipulations to rewire cellular metabolism primarily with the aim to transform the organism into a single-celled chemical factory. Using biological processes, we can produce more complex chemicals in a more sustainable way. This is particularly important...
journal_title：FEMS microbiology letters
pub_type： 杂志文章,评审
doi：10.1093/femsle/fny079
更新日期：2018-05-01 00:00:00
abstract：:The structure and distribution of a Mycobacterium bovis BCG insertion element of the IS21 family were investigated. Several IS21-like elements found in mycobacterial genomes were separated in four types, following their nucleic acid similarities. The M. bovis BCG IS21 element is highly similar to IS1533 (class I), 70%...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1999.tb08679.x
更新日期：1999-09-15 00:00:00
abstract：:The adhesion-promoting proteins (APP) (molecular mass approx. 300 kDa), which promote adhesion of Streptococcus mutans JBP (serotype c) to hydroxyapatite, were isolated from human submandibular-sublingual (SMSL) saliva by gel filtration on a Trisacryl GP2000 M column. The effects of hexoses, pentoses, methyl-pentoses,...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/0378-1097(91)90639-r
更新日期：1991-12-15 00:00:00
abstract：:Carbohydrate-binding components were shown to be present at the surface of Listeria monocytogenes by means of a panel of neoglycoproteins using direct agglutination. These lectin-like components bind on neoglycoproteins bearing D-glucosamine, L-fucosylamine, or para-amino-phenyl-alpha-D-mannopyrannoside residues. The ...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/s0378-1097(05)80058-8
更新日期：1990-03-15 00:00:00
abstract：:Xylose reductase catalyzes the NAD(P)H-dependent reduction of xylose to xylitol and is essential for growth on xylose by yeasts. To understand the nature of coenzyme binding to the Pichia stipitis xylose reductase, we investigated the role of the strictly conserved Lys270 in the putative IPKS coenzyme binding motif by...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1998.tb12848.x
更新日期：1998-02-01 00:00:00
abstract：:Wood-rotting fungi possess remarkably diverse extracellular oxidation mechanisms, including enzymes, such as laccase and peroxidases, and Fenton chemistry. The ability to biologically drive Fenton chemistry by the redox cycling of quinones has previously been reported to be present in both ecologically diverging main ...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1093/femsle/fnw105
更新日期：2016-06-01 00:00:00
abstract：:Yeast two-hybrid and Far Western analyses were used to detect interactions between Bacillus subtilis spores' nutrient germinant receptor proteins and proteins encoded by the spoVA operon, all of which are involved in spore germination and located in the spores' inner membrane. These analyses indicated that two subunit...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2007.00807.x
更新日期：2007-09-01 00:00:00
abstract：:Intracellular copper routing in Enterococcus hirae is accomplished by the CopZ copper chaperone. Under copper stress, CopZ donates Cu(+) to the CopY repressor, thereby releasing its bound zinc and abolishing repressor-DNA interaction. This in turn induces the expression of the cop operon, which encodes CopY and CopZ, ...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2009.01833.x
更新日期：2010-01-01 00:00:00
abstract：:A suite of six sterols, lanosterol, lanost-8(9)-en-3beta-ol, 4, 4-dimethylcholesta-8(14),24-dien-3beta-ol, 4, 4-dimethylcholest-8(14)-en-3beta-ol, 4-methylcholesta-8(14), 24-dien-3beta-ol and 4-methylcholest-8(14)-en-3beta-ol, were identified in the psychrophilic methanotrophic bacterium, Methylosphaera hansonii. Thei...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2000.tb09103.x
更新日期：2000-05-15 00:00:00
abstract：:Probing protein extracts from exponentially growing and stationary phase cultures of Mycobacterium bovis BCG with anti-phospho amino acid antibodies revealed a 31-kDa anti-phospho threonine antibody-reactive protein specific to growing culture. The corresponding protein was purified via two-dimensional gel electrophor...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/S0378-1097(03)00664-5
更新日期：2003-10-10 00:00:00
abstract：:Among aerobic prokaryotes, many different terminal oxidase complexes have been described. Sequence comparison has revealed that the aa3-type cytochrome c oxidase and the bo3-type quinol oxidase are variations on the same theme: the heme-copper oxidase. A third member of this family has recently been recognized: the cb...
journal_title：FEMS microbiology letters
pub_type： 杂志文章,评审
doi：10.1111/j.1574-6968.1994.tb07067.x
更新日期：1994-08-01 00:00:00
abstract：:The riboflavin kinase encoding gene ribR is situated within a 12 genes locus ytmI-ytnM of the Bacillus subtilis chromosome. Here we demonstrate that ribR is transcribed as part of a 10 kb ytmI-ytnM operon. The riboflavin overproduction phenotype of B. subtilis ribC mutant strains, which is a result of the strongly red...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/j.femsle.2004.11.038
更新日期：2005-02-01 00:00:00
abstract：:Two fungal isolates, formerly classified as Verticillium alboatrum and proposed as forming the basis of a new sub-group ('Group 2') within the species, have been shown to be non-pathogenic to known hosts of V. alboatrum and, on the basis of molecular evidence, to be closely related to Verticillium psalliotae and Verti...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1999.tb13376.x
更新日期：1999-01-01 00:00:00
abstract：:Antimicrobial resistance (AMR) has emerged as one of the most pressing threats to public health. AMR evolution occurs in the clinic but also in the environment, where antibiotics and heavy metals can select and co-select for AMR. While the selective potential of both antibiotics and metals is increasingly well-charact...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1093/femsle/fnaa038
更新日期：2020-02-01 00:00:00
abstract：:Legionella pneumophila is a pathogenic bacterium found in freshwater environments that is responsible for pneumonia. People become infected through inhalation of contaminated droplets from water devices, such as cooling towers and showers. It is important to find new treatments that decrease the development of Legione...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/j.femsle.2005.03.046
更新日期：2005-11-01 00:00:00
abstract：:The branched-chain amino acid permease Bap2p is a transport system for leucine, isoleucine, and valine in Saccharomyces cerevisiae, and its synthesis is regulated transcriptionally. However, the downregulation mechanisms of Bap2p have not been established. Here we demonstrate that the C-terminal region of Bap2p plays ...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2001.tb09471.x
更新日期：2001-01-15 00:00:00
abstract：:Type II restriction endonucleases were purified by heparin-sepharose followed by ion chromatography from Treponema strains. The results indicate that in addition to frequently cutting GATC-specific restriction enzymes, the tested strains also possess rarely cutting endonucleases. The purified restriction endonucleases...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/j.femsle.2005.07.032
更新日期：2005-10-01 00:00:00
abstract：:The presence of diatoms in victim's internal organs has been regarded as a gold biological evidence of drowning. The idea becomes true at the advent of DNA metabarcoding. Unfortunately, the DNA barcode of diatoms are far from being applicable due to neither consensus on the barcode and nor reliable reference library.I...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1093/femsle/fnaa145
更新日期：2020-09-01 00:00:00
abstract：:The photoinduced bactericidal capacity of TiO(2) based films was evaluated, using as model organism Pseudomonas aeruginosa. Thin films were obtained by spray pyrolysis; they included undoped, Cu doped, and Al doped TiO(2). Scanning electron microscopy was used to observe the final effect of the irradiated films upon t...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2002.tb11222.x
更新日期：2002-06-04 00:00:00
abstract：:We have analyzed 81 isolates of Mycoplasma agalactiae from four different regions of Italy between 1990 and 1995 in order to identify antigenic differences through SDS-PAGE and Western blotting and chromosomal DNA restriction endonuclease cleavage pattern differences. Antigenic variability in M. agalactiae isolates wa...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1996.tb08490.x
更新日期：1996-10-01 00:00:00
abstract：:Chlamydophila pneumoniae infection is associated with a range of diseases including pneumonia, asthma and heart disease. Although an obligate intracellular pathogen, high levels of antigen-specific antibody are induced and serology is frequently used to diagnose these infections. Proteins targeted by the humoral respo...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/S0378-1097(03)00650-5
更新日期：2003-10-10 00:00:00
abstract：:A phage typing system using a group of 11 closely related phage (as judged by Southern analysis and restriction fragment length polymorphism analysis) was able to distinguish at least six phage types in Salmonella heidelberg of human and animal origin. Restriction fragment length polymorphism analysis using cosmid pro...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1993.tb06118.x
更新日期：1993-04-15 00:00:00
abstract：:Pneumocystis is a parasitic fungus causing pneumonia in immunosuppressed mammals and S-adenosylmethionine a key intermediary metabolite for all cells. Other than a species of Rickettsia bacteria and an aberrant strain of the protozoan Amoeba proteus, Pneumocystis is the only cell known unable to synthesize AdoMet; it ...
journal_title：FEMS microbiology letters
pub_type： 杂志文章,评审
doi：10.1016/j.femsle.2004.06.039
更新日期：2004-08-15 00:00:00
abstract：:Riemerella anatipestifer is the causative agent of polyserositis of ducks and geese. We have previously reported that a 3.9-kb plasmid, pCFC1, carries protein genes (vapD1 and vapD2) that are similar to virulence-associated genes of other bacteria. In the present study, we report the complete sequence of a second plas...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1999.tb08701.x
更新日期：1999-10-01 00:00:00
abstract：:Understanding drug resistance in Mycobacterium tuberculosis requires an integrated analysis of strain lineages, mutations and gene expression. Previously, we reported the differential expression of esxG, esxH, infA, groES, rpmI, rpsA and lipF genes in a sensitive M. tuberculosis strain and in a multidrug-resistant cli...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1093/femsle/fnv194
更新日期：2015-12-01 00:00:00
abstract：:Streptococcus pneumoniae strain JNR.7/87 is a highly virulent, type 4 encapsulated Gram-positive bacterium whose transformability has not been tested previously, and whose genome is currently being sequenced. The strain was transformed at very low efficiency by addition of exogenous competence-stimulating peptide: How...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1999.tb13460.x
更新日期：1999-03-15 00:00:00
abstract：:High resistance to class IIa bacteriocins in Listeria monocytogenes has been clearly linked to lack of expression of the mptACD operon, encoding the EIIt Man mannose PTS permease. Also, intermediate resistance has been associated with membrane phospholipid modifications in the spontaneous mutants L. monocytogenes B73-...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/j.femsle.2004.07.014
更新日期：2004-09-01 00:00:00
abstract：:The diheme cytochromes c of the widespread TsdA family are bifunctional thiosulfate dehydrogenase/tetrathionate reductases. Here, biochemical information was collected about TsdA from the Epsilonproteobacterium Wolinella succinogenes (WsTsdA). The situation in W. succinogenes is unique since TsdA is closely associated...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1093/femsle/fnx003
更新日期：2017-02-01 00:00:00
abstract：:To investigate the regulatory mechanism governing antifungal metabolite biosynthesis, two kinds of global regulator genes in Pseudomonas sp. M18, an rpoS and an rsmA gene, were cloned and mutated by inserting with an aacC1 cassette, respectively. Two mutants showed the same regulatory mode with repression of phenazine...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2006.00562.x
更新日期：2007-03-01 00:00:00
abstract：:IS1(SB) and its two variants were identified as the major and minor IS1 elements in Shigella boydii. The nucleotide sequences of IS1(SB), IS1(O157:H7) from Escherichia coli O157:H7 and IS1F from E. coli K12 suggest that these IS1 elements had been horizontally transferred among S. boydii and E. coli O157:H7 and K12. T...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/S0378-1097(03)00319-7
更新日期：2003-05-28 00:00:00
