Abstract:
:Mini-Tn10luxABcam/Ptac-ATS was constructed in order to develop a luciferase-transducing bacteriophage for detecting Escherichia coli O157:H7. The transposon was designed to deliver a 3.6-kb insertion that confers n-decanal-dependent bioluminescence and resistance to chloramphenicol and was constructed using mini-Tn10cam/Ptac-ATS in the plasmid pNK2884 and luxAB from Vibrio harveyi. PhiV10, a temperate bacteriophage infecting common phage types of Escherichia coli O157:H7, was mutagenized as a prophage in E. coli O157:H7 strain R508. PhiV10::luxABcamA1-23 was rescued from the strain by propagating it on a strain lacking the bacteriophage and the vector containing the transposon. The bacteriophage transduced n-decanal-dependent bioluminescence to E. coli O157:H7 strain R508 that was measurable approximately 1 h post infection.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Waddell TE,Poppe Cdoi
10.1111/j.1574-6968.2000.tb08909.xkeywords:
subject
Has Abstractpub_date
2000-01-15 00:00:00pages
285-9issue
2eissn
0378-1097issn
1574-6968pii
S0378-1097(99)00603-5journal_volume
182pub_type
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