Siro(haem)amide in Allochromatium vinosum and relevance of DsrL and DsrN, a homolog of cobyrinic acid a,c-diamide synthase, for sulphur oxidation.

Abstract:

:In the purple sulphur bacterium Allochromatium vinosum, the prosthetic group of dissimilatory sulphite reductase (DsrAB) was identified as siroamide, an amidated form of the classical sirohaem. The genes dsrAB are the first two of a large cluster of genes necessary for the oxidation of sulphur globules stored intracellularly during growth on sulphide and thiosulphate. DsrN is homologous to cobyrinic acid a,c diamide synthase and may therefore catalyze glutamine-dependent amidation of sirohaem. Indeed, an A. vinosumDeltadsrN in frame deletion mutant showed a significantly reduced sulphur oxidation rate that was fully restored upon complementation with dsrN in trans. Sulphite reductase was still present in the DeltadsrN mutant. DsrL is a homolog of the small subunits of bacterial glutamate synthases and was proposed to deliver glutamine for sirohaem amidation. However, recombinant DsrL does not exhibit glutamate synthase activity nor does the gene complement a glutamate synthase-deficient Escherichia coli strain. Deletion of dsrL showed that the encoded protein is absolutely essential for sulphur oxidation in A. vinosum.

journal_name

FEMS Microbiol Lett

authors

Lübbe YJ,Youn HS,Timkovich R,Dahl C

doi

10.1111/j.1574-6968.2006.00343.x

subject

Has Abstract

pub_date

2006-08-01 00:00:00

pages

194-202

issue

2

eissn

0378-1097

issn

1574-6968

pii

FML343

journal_volume

261

pub_type

杂志文章
  • A new pullulan and a branched (1-->3)-, (1-->6)-linked beta-glucan from the lichenised ascomycete Teloschistes flavicans.

    abstract::The polysaccharides formed on hot alkaline extraction of the ascomycetous lichen Teloschistes flavicans were fractionated to give two glucans, which were characterised by methylation analysis and 1D and 2D NMR spectroscopy. One was a branched beta-glucan containing (1-->3) and (1-->6) linkages, a structure which is mo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11152.x

    authors: Reis RA,Tischer CA,Gorin PA,Iacomini M

    更新日期:2002-04-23 00:00:00

  • Differentiation of enterococci from other group D streptococci by means of a specific monoclonal antibody.

    abstract::We report on the specificity of a monoclonal antibody which reacts with autoclaved extracts of four species of enterococci but does not react to the same extent with similar extracts from two non-enterococcal group D streptococci. The monoclonal antibody also reacts specifically with purified lipoteichoic acid from St...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(91)90426-b

    authors: Hammonds SJ,Easterbrook TJ,McIllmurray MB

    更新日期:1991-07-15 00:00:00

  • Characterization of SCAR markers of Eimeria spp. of domestic fowl and construction of a public relational database (The Eimeria SCARdb).

    abstract::This study reports the development and characterization of 151 sequence characterized amplified region (SCAR) markers for the seven Eimeria species that infect the domestic fowl. From this set, 84 markers are species-specific and 67 present partial specificity. The complete nucleotide sequence was derived for all mark...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.07.034

    authors: Fernandez S,Katsuyama AM,Kashiwabara AY,Madeira AM,Durham AM,Gruber A

    更新日期:2004-09-01 00:00:00

  • Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: towards an understanding of the rules governing complex formation and thereby functional flexibility.

    abstract::We have previously shown that the secreted M. tuberculosis complex proteins CFP-10 and ESAT-6 form a tight, 1:1 complex, which may represent their functional form. In the work reported here a combination of yeast two-hybrid and biochemical analysis has been used to characterise complex formation between two other pair...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.07.043

    authors: Lightbody KL,Renshaw PS,Collins ML,Wright RL,Hunt DM,Gordon SV,Hewinson RG,Buxton RS,Williamson RA,Carr MD

    更新日期:2004-09-01 00:00:00

  • Successful enrichment of low-abundant comammox Nitrospira from nitrifying granules under ammonia-limited conditions.

    abstract::In artificial engineered systems, nitrification is a key reaction that accounts for the removal of biological nitrogen. Recently, a single microbe capable of oxidizing ammonia to nitrate, known as a complete ammonia oxidizer (comammox), has been discovered. Although the abundance and diversity of comammox Nitrospira i...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnaa025

    authors: Fujitani H,Nomachi M,Takahashi Y,Hasebe Y,Eguchi M,Tsuneda S

    更新日期:2020-01-01 00:00:00

  • The IS1 elements in Shigella boydii: horizontal transfer, vertical inactivation and target duplication.

    abstract::IS1(SB) and its two variants were identified as the major and minor IS1 elements in Shigella boydii. The nucleotide sequences of IS1(SB), IS1(O157:H7) from Escherichia coli O157:H7 and IS1F from E. coli K12 suggest that these IS1 elements had been horizontally transferred among S. boydii and E. coli O157:H7 and K12. T...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00319-7

    authors: Hsu WB,Chen JH

    更新日期:2003-05-28 00:00:00

  • Detection and quantification of virulent Aeromonas hydrophila in channel catfish tissues following waterborne challenge.

    abstract::The aim of this study was to understand the pathogenesis of motile aeromonas septicemia caused by an emergent, high virulent Aeromonas hydrophila (vAh) in channel catfish, Ictalurus punctatus Adipose fin clipped catfish were challenged with vAh using a waterborne challenge method, and the distribution of vAh over a ti...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnw080

    authors: Zhang D,Moreira GS,Shoemaker C,Newton JC,Xu DH

    更新日期:2016-05-01 00:00:00

  • Sample sequencing of a Salmonella typhimurium LT2 lambda library: comparison to the Escherichia coli K12 genome.

    abstract::As part of the ongoing sequencing of the complete Salmonella typhimurium LT2 genome, a partly ordered set of 416 lambda clones has been developed, representing over 90% of the genome. The average insert size is 17 kb. Sequences were obtained from both ends of each clone in this set. A total of over 600 kb of sequence ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13533.x

    authors: Wong RM,Wong KK,Benson NR,McClelland M

    更新日期:1999-04-15 00:00:00

  • Spreading the message of antimicrobial resistance: a detailed account of a successful public engagement event.

    abstract::The increase in Antimicrobial resistance (AMR) microorganisms has been exacerbated by exposure to antimicrobial drugs (e.g. antibiotics). A solution to AMR may require academic researchers to not only contribute to the drug discovery pipeline through laboratory research, but also to engage and inform non-specialist au...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fny175

    authors: Redfern J,Bowater L,Crossley M,Verran J

    更新日期:2018-08-01 00:00:00

  • Functional characterization of the competence protein DprA/Smf in Escherichia coli.

    abstract::In several bacterial species that show natural transformation, dprA has been described as a competence gene. The DprA protein has been suggested to be involved in the protection of incoming DNA. However, members of the dprA gene family (also called smf) can be detected in virtually all bacterial species, which suggest...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00423.x

    authors: Smeets LC,Becker SC,Barcak GJ,Vandenbroucke-Grauls CM,Bitter W,Goosen N

    更新日期:2006-10-01 00:00:00

  • Increased expression and hotspot mutations of the multidrug efflux transporter, CDR1 in azole-resistant Candida albicans isolates from vaginitis patients.

    abstract::The aims of our research were to investigate the gene expression of the multidrug efflux transporter, CDR1 and the major drug facilitator superfamily transporter, MDR1 gene in azole drug-resistant Candida albicans and Candida glabrata clinical isolates recovered from vaginitis patients; and to identify hotspot mutatio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.06.036

    authors: Looi CY,D' Silva EC,Seow HF,Rosli R,Ng KP,Chong PP

    更新日期:2005-08-15 00:00:00

  • Antibacterial effects of protruding and recessed shark skin micropatterned surfaces of polyacrylate plate with a shallow groove.

    abstract::Antibacterial effects in terms of biofilm formation and swarming motility were studied using polyacrylate plates having protruding or recessed shark skin micropatterned surfaces with a shallow groove (2 μm pattern width and spacing, 0.4 μm pattern height). It was found that biofilm formation and swarming motility of P...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12604

    authors: Sakamoto A,Terui Y,Horie C,Fukui T,Masuzawa T,Sugawara S,Shigeta K,Shigeta T,Igarashi K,Kashiwagi K

    更新日期:2014-12-01 00:00:00

  • Characterization of extended-spectrum-beta-lactamase (CTX-M-15)-producing strains of Salmonella enterica isolated in France and Senegal.

    abstract::Three clinical isolates of Salmonella enterica recovered between 2000 and 2003 in France and Senegal were found to produce extended-spectrum beta-lactamase CTX-M-15. The two isolates from Senegal were recovered from stool of a hospitalized patient with gastroenteritis in 2000 and from an urine specimen of an out-patie...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.07.058

    authors: Weill FX,Perrier-Gros-Claude JD,Demartin M,Coignard S,Grimont PA

    更新日期:2004-09-15 00:00:00

  • Roseovarius sp. strain 217: aerobic taurine dissimilation via acetate kinase and acetate-CoA ligase.

    abstract::The genome sequence of Roseovarius sp. strain 217 indicated that many pathway enzymes found in other organisms for the degradation of taurine are represented, but that a novel, apparently energy-dependent pathway is involved in the conversion of acetyl phosphate to acetyl CoA. Thus, an ABC transporter for taurine coul...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00719.x

    authors: Baldock MI,Denger K,Smits TH,Cook AM

    更新日期:2007-06-01 00:00:00

  • Aerobic biodegradation of benzene and toluene under hypersaline conditions at the Great Salt Plains, Oklahoma.

    abstract::The Great Salt Plains is a 65-km(2) hypersaline habitat of geological origin located in north-central Oklahoma. Contamination of such ecosystems by petroleum compounds is expected from non-point sources and due to increased human activities. Little information exists about the ability of halophilic and halotolerant ba...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.03.014

    authors: Nicholson CA,Fathepure BZ

    更新日期:2005-04-15 00:00:00

  • Secretory production of Arthrobacter levan fructotransferase from recombinant Escherichia coli.

    abstract::Levan fructotransferase (LFTase) from Arthrobacter ureafaciens K2032 was expressed with N-terminal fusion of a LacZ-derived secretion motif (TMITNSSSVP) using the lac promoter system in recombinant Escherichia coli JM109 [pUDF-A81]. In flask cultures, recombinant enzyme activity was detected in culture media, and sequ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10509.x

    authors: Lee J,Saraswat V,Koh I,Song KB,Park YH,Rhee SK

    更新日期:2001-02-20 00:00:00

  • Characterization of the membrane sensor PenJ for beta-lactam antibiotics from Bacillus licheniformis by amino acid substitution.

    abstract::The PenJ protein of the penicillinase gene (penP) expression system from Bacillus licheniformis is an antirepressor and membrane receptor for beta-lactam antibiotics. A putative beta-lactam antibiotic binding site including Ser402 and Lys405, which are homologous to the conserved sequence for the beta-lactam binding s...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06306.x

    authors: Takagi M,Ohta T,Johki S,Imanaka T

    更新日期:1993-06-01 00:00:00

  • Comparison of SEM and VPSEM imaging techniques with respect to Streptococcus mutans biofilm topography.

    abstract::The study compared images of mature Streptococcus mutans biofilms captured at increasing magnification to determine which microscopy method is most acceptable for imaging the biofilm topography and the extracellular polymeric substance (EPS). In vitro S. mutans biofilms were imaged using (1) scanning electron microsco...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/1574-6968.12334

    authors: Weber K,Delben J,Bromage TG,Duarte S

    更新日期:2014-01-01 00:00:00

  • The impact factor: implications for research policy, editorial rules and scholarly reputation.

    abstract::The assessment of scientific research is essentially based on several metric parameters, the so-termed Impact Factor perhaps being the predominant one. Despite well-founded criticisms and the wide opposition of reputed scientists, this procedure has become a tool of scientific policy, and is applied in editorial proce...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz132

    authors: Argüelles JC,Argüelles-Prieto R

    更新日期:2019-06-01 00:00:00

  • Characterization of a recombinant transferrin-binding protein A (TbpA) fragment from Haemophilus parasuis serovar 5.

    abstract::Haemophilus parasuis, the etiological agent of Glässer's disease in pigs, possesses iron acquisition pathways mediated by a surface receptor that specifically bind porcine transferrin. This receptor is composed of transferrin-binding protein A (TbpA) and TbpB. As it has been reported for other gram-negative organisms,...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.01970.x

    authors: Martínez S,Frandoloso R,Rodríguez-Ferri EF,González-Zorn B,Gutiérrez-Martín CB

    更新日期:2010-06-01 00:00:00

  • Iterative selection from a Salmonella typhimurium cosmid library can lead to the isolation of an atypically small plasmid.

    abstract::We have constructed a DNA library from a virulent Salmonella typhimurium strain, in an avirulent strain. The process of selecting the components of interest from the library involved iterative growth in liquid culture. This resulted, after four cycles, in the culture becoming homogeneous for a single plasmid, which wa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13395.x

    authors: Lodge JM,Robey M,Stephen J,Brown NL

    更新日期:1999-01-15 00:00:00

  • Predicting sigma28 promoters in eleven Shewanella genomes.

    abstract::An iterative position-specific score matrix (PSSM)-based approach was used to predict sigma(28) promoters in 11 Shewanella genomes. The Shewanella Correlation Browser was used to distinguish true-positive predictions from false-positive predictions in Shewanella oneidensis MR-1 by generating a sigma(28)-regulated tran...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01175.x

    authors: Song W,Juhn FS,Naiman DQ,Konstantinidis KT,Gardner TS,Ward MJ

    更新日期:2008-06-01 00:00:00

  • Comparison of bacterial flora and enzymatic activity in faeces of infants and calves.

    abstract::Sixty-four breast-fed infants and 23 calves were investigated for bacteria and enzymatic activity in their faecal samples. The bacteria were measured using cultivation and fluorescence in situ hybridization. Enzymatic activity was also examined. Forty-seven (64%) infants and all the calves had high numbers of bifidoba...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00207.x

    authors: Rada V,Vlková E,Nevoral J,Trojanová I

    更新日期:2006-05-01 00:00:00

  • Purification and characterization of Bordetella calmodulin-like protein.

    abstract::Bordetella calmodulin-like protein was purified from culture supernatant fluid of B. pertussis, B. parapertussis and B. bronchiseptica by successive chromatography on hydroxyapatite, Toyopearl HW-50F and QAE-Toyopearl 550C columns. The purified calmodulin-like protein appeared to be homogeneous by SDS-polyacrylamide g...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb06696.x

    authors: Nagai M,Endoh M,Danbara H,Nakase Y

    更新日期:1994-02-15 00:00:00

  • OK, thanks! A new mutualism between Chlamydomonas and methylobacteria facilitates growth on amino acids and peptides.

    abstract::Nitrogen is a key nutrient for land plants and phytoplankton in terrestrial and aquatic ecosystems. The model alga Chlamydomonas reinhardtii can grow efficiently on several inorganic nitrogen sources (e.g. ammonium, nitrate, nitrite) as well as many amino acids. In this study, we show that Chlamydomonas is unable to u...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fny021

    authors: Calatrava V,Hom EFY,Llamas Á,Fernández E,Galván A

    更新日期:2018-04-01 00:00:00

  • Transcription and genetic analyses of a putative N-acetylmuramyl-L-alanine amidase in Borrelia burgdorferi.

    abstract::In this study, a putative N-acetylmuramyl-l-alanine amidase gene (bb0666) was identified in the genome of the Lyme disease spirochete Borrelia burgdorferi. This protein shares c. 30% identity with its counterparts from other bacteria. Reverse transcriptase-PCR analysis showed that bb0666 along with two other genes (bb...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01416.x

    authors: Yang Y,Li C

    更新日期:2009-01-01 00:00:00

  • Identification and characterization of GroEL and DnaK homologues in Thiobacillus ferrooxidans.

    abstract::The major heat shock proteins from Thiobacillus ferrooxidans were identified as DnaK and GroEL equivalents by Western blotting and analysis of the N-terminal amino acid sequence of spots isolated from dried 2-D polyacrylamide electrophoresis gels. The T. ferrooxidans chaperonins showed 70% and 80% identity with the Es...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90147-g

    authors: Varela P,Jerez CA

    更新日期:1992-11-01 00:00:00

  • A surface polysaccharide forms when gonococci are converted to serum resistance by cytidine 5'-monophospho-N-acetyl neuraminic acid.

    abstract::A serum-susceptible, guinea-pig chamber-passaged, laboratory strain (BS4 (agar)) of Neisseria gonorrhoeae was converted to serum resistance by incubation with cytidine 5-monophospho-N-acetyl neuraminic acid (CMP-NANA) and examined by electron microscopy after staining with ruthenium-red. In contrast to serum susceptib...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90261-n

    authors: Fox AJ,Curry A,Rowland PL,Lancaster S,Jones DM,Parsons NJ,Cole JA,Smith H

    更新日期:1990-01-01 00:00:00

  • The Aspergillus niger acuA and acuB genes correspond to the facA and facB genes in Aspergillus nidulans.

    abstract::Mutants in Aspergillus niger unable to grow on acetate as a sole carbon source were previously isolated by resistance to 1.2% propionate medium containing 0.1% glucose. AcuA mutants lacked acetyl-CoA synthetase (ACS) activity and acuB mutants lacked both ACS and isocitrate lyase activity. An acuA mutant was transforme...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13756.x

    authors: Papadopoulou S,Sealy-Lewis HM

    更新日期:1999-09-01 00:00:00

  • Metabolism of phenylbenzoate by Pseudomonas sp. strain TR3.

    abstract::A bacterial isolate, tentatively identified as Pseudomonas sp. strain TR3, was found to utilize the diaryl ester phenylbenzoate as sole source of carbon and energy. This strain has the ability to productively degrade phenylbenzoate and some substituted derivatives by a catabolic sequence which was characterized bioche...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13700.x

    authors: Reich T,Schmidt S,Fortnagel P

    更新日期:1999-07-15 00:00:00