Abstract:
:This study reports the development and characterization of 151 sequence characterized amplified region (SCAR) markers for the seven Eimeria species that infect the domestic fowl. From this set, 84 markers are species-specific and 67 present partial specificity. The complete nucleotide sequence was derived for all markers, revealing the presence of micro- and minisatellite repetitive units in 22 SCARs, with up to five distinct repeat units being observed per marker. Only 15 markers showed significant hits in similarity searches against public sequence databases, thus confirming their anonymous and non-coding character. Finally, a relational database of the markers (the Eimeria SCARdb) was developed and made available on the Internet, providing a valuable resource of SCAR markers that can be useful for molecular diagnosis, and also for epizootiological, genetic variability and genome mapping studies.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Fernandez S,Katsuyama AM,Kashiwabara AY,Madeira AM,Durham AM,Gruber Adoi
10.1016/j.femsle.2004.07.034keywords:
subject
Has Abstractpub_date
2004-09-01 00:00:00pages
183-8issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(04)00538-5journal_volume
238pub_type
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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abstract::A number of hybrids obtained by fusion of protoplasts of complementary auxotrophic strains of Candida albicans were analysed for growth rates, cell volume, DNA content, stability and adherence to human buccal epithelial cells. SDS-PAGE analysis of the cell wall proteins indicated that the hybrid cell walls contained m...
journal_title:FEMS microbiology letters
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