Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: towards an understanding of the rules governing complex formation and thereby functional flexibility.

abstract：:The ability of 14 Lactobacillus strains, isolated from fermented dry sausages, to transfer tetracycline resistance encoded by tet(M) through conjugation was examined using filter mating experiments. Seven out of 14 tetracycline-resistant Lactobacillus isolates were able to transfer in vitro this resistance to Enteroco...

journal_title：FEMS microbiology letters

authors： Gevers D,Huys G,Swings J

更新日期：2003-08-08 00:00:00

abstract：:Total dsRNA extractions in five killer K2 strains of Saccharomyces cerevisiae isolated from spontaneous fermentations revealed the presence of a novel dsRNA fragment (which we named NS dsRNA) of approximately 1.30 kb, together with L and M2 dsRNAs. NS dsRNA appeared to be encapsidated in the same kind of viral particl...

journal_title：FEMS microbiology letters

authors： Cansado J,Barros Velázquez J,Sieiro C,Gacto M,Villa TG

更新日期：1999-12-15 00:00:00

abstract：:Clostridium difficile and Clostridium perfringens type A are infectious agents of enteric diseases. The main virulence factors of these microorganisms include toxins A and B of C. difficile (ToxA and ToxB) and enterotoxin of C. perfringens (Cpe). In this study genetic constructions have been created for the expression...

journal_title：FEMS microbiology letters

authors： Belyi IF,Varfolomeeva NA

更新日期：2003-08-29 00:00:00

abstract：:Chemical and molecular taxonomic studies were performed on a representative strain of some lactic acid bacteria of unknown taxonomic position isolated from salmonid fish. The results demonstrate that the fish bacterium represents a new species of the genus Lactococcus for which the name Lactococcus piscium sp. nov. is...

journal_title：FEMS microbiology letters

authors： Williams AM,Fryer JL,Collins MD

更新日期：1990-03-01 00:00:00

abstract：:To identify antigens that would improve the accuracy of serological diagnosis of active tuberculosis, we cloned the genes encoding nine potentially immunogenic secreted or surface-associated proteins of Mycobacterium tuberculosis. Recombinant proteins were reacted with sera from HIV-negative individuals with extrapulm...

journal_title：FEMS microbiology letters

authors： Moran AJ,Treit JD,Whitney JL,Abomoelak B,Houghton R,Skeiky YA,Sampaio DP,Badaró R,Nano FE

更新日期：2001-04-20 00:00:00

abstract：:Although very little, if any, beta-galactosidase activity is detected in Yersinia pestis by a standard Miller assay, we found that Y. pestis KIM6+ cells formed blue colonies on plates containing 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal). Searches of the Y. pestis genome databases revealed the presence of n...

journal_title：FEMS microbiology letters

authors： Bobrov AG,Perry RD

更新日期：2006-02-01 00:00:00

abstract：:Bacteria have the exquisite ability to maintain a precise diameter, cell length, and shape. The dimensions of bacteria size and shape are a classical metric in the distinction of bacterial species. Much of what we know about the particular morphology of any given species is the result of investigations of planktonic c...

journal_title：FEMS microbiology letters

authors： Justice SS,Harrison A,Becknell B,Mason KM

更新日期：2014-11-01 00:00:00

abstract：:Macrolide is inactivated with ATP plus crude extract of Escherichia coli producing macrolide 2'-phosphotransferase (MPH(2')), but not by living cells. Therefore, a convenient method for detection of MPH(2') using intact cells is needed. In this report, we determine that the modified lysozyme-DNase-RNase (LDR) method (...

journal_title：FEMS microbiology letters

authors： Taniguchi K,Nakamura A,Tsurubuchi K,O'Hara K,Sawai T

更新日期：1998-10-15 00:00:00

abstract：:D-(-)-3-Hydroxybutyrate (3HB) oligomer hydrolase was purified from Paracoccus denitrificans. The enzyme was a monomeric protein with an approximate molecular mass of 31 kDa. The isoelectric point of the enzyme was 5.2. Optimum temperature and pH were 35-40 degrees C and 8.0, respectively. The enzyme activity was not a...

journal_title：FEMS microbiology letters

authors： Ueda S,Sano K,Gao D,Tomihari N,Yamane T,Endo I

更新日期：2002-01-10 00:00:00

abstract：:When Escherichia coli STII was applied to the serosa of the ileum at a concentration of 40 micrograms/ml, an acceleration of the spontaneous motility and a weak contraction were induced 2-3 min later. The induction was not affected by the addition of atropine (10(-6) M), but was abolished by the addition of papaverine...

journal_title：FEMS microbiology letters

authors： Hitotsubashi S,Akagi M,Saitou A,Yamanaka H,Fujii Y,Okamoto K

更新日期：1992-01-15 00:00:00

abstract：:Methods for in vivo monitoring of redox changes in different cellular compartments have been developed in recent years, and are mostly based on redox-sensitive variants of the green fluorescent protein (GFP). However, due to the thermodynamic stability of the introduced reactive disulfide bond, these sensors are limit...

journal_title：FEMS microbiology letters

authors： Delic M,Mattanovich D,Gasser B

更新日期：2010-05-01 00:00:00

abstract：:Diesel fuel is a common environmental pollutant comprised of a large number of both aromatic and aliphatic hydrocarbons. The microbial degradation of individual hydrocarbons has been well characterized, however, the community dynamics within a system degrading a complex pollutant such as diesel fuel are still poorly u...

journal_title：FEMS microbiology letters

authors： Ciric L,Philp JC,Whiteley AS

更新日期：2010-02-01 00:00:00

abstract：:The discovery of nitrite-dependent anaerobic methane oxidation (n-damo) mediated by 'Candidatus Methylomirabilis oxyfera' with nitrite and methane as substrates has connected biogeochemical carbon and nitrogen cycles in a new way. The paddy fields often carry substantial methane and nitrate, thus may be a favorable ha...

journal_title：FEMS microbiology letters

authors： Zhou L,Wang Y,Long XE,Guo J,Zhu G

更新日期：2014-11-01 00:00:00

abstract：:To explore the discriminatory power of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for detecting subtle differences in isogenic isolates, we tested isogenic strains of Staphylococcus aureus differing in their expression of resistance to methicillin or teicoplanin. More i...

journal_title：FEMS microbiology letters

authors： Majcherczyk PA,McKenna T,Moreillon P,Vaudaux P

更新日期：2006-02-01 00:00:00

abstract：:A metagenomic approach was taken to investigate the genetic basis for the ability of an anaerobic consortium to grow on either 4-chlorobenzoate or 4-bromobenzoate under denitrifying conditions. Degenerate PCR primers were designed for the family of 4-chlorobenzoyl-CoA dehalogenase genes. The primers were utilized to s...

journal_title：FEMS microbiology letters

authors： Chae JC,Song B,Zylstra GJ

更新日期：2008-04-01 00:00:00

abstract：:A total of 401 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates from two experimentally infected calves were analyzed using molecular biological methods. Genetic differences detected by pulsed-field gel electrophoresis were observed between the inoculated and recovered strains as early as 1 day post inoculat...

journal_title：FEMS microbiology letters

authors： Akiba M,Sameshima T,Nakazawa M

更新日期：2000-03-01 00:00:00

abstract：:MHYT, a new conserved protein domain with a likely signaling function, is described. This domain consists of six transmembrane segments, three of which contain conserved methionine, histidine, and tyrosine residues that are projected to lie near the outer face of the cytoplasmic membrane. In Synechocystis sp. PCC6803,...

journal_title：FEMS microbiology letters

authors： Galperin MY,Gaidenko TA,Mulkidjanian AY,Nakano M,Price CW

更新日期：2001-11-27 00:00:00

abstract：:How proteins reach their native conformation and location has been a major question of biology during the last 50 years. To counterbalance protein misfolding and the accumulation of aggregation products, a complex network of chaperones and proteases takes care of protein quality control in the cell. Such a chaperone n...

journal_title：FEMS microbiology letters

authors： Mas G,Hiller S

更新日期：2018-07-01 00:00:00

abstract：:Two cDNAs encoding feedback inhibition-insensitive serine acetyltransferases of Arabidopsis thaliana were expressed in the chromosomal serine acetyltransferase-deficient and L-cysteine non-utilizing Escherichia coli strain JM39-8. The transformants produced 1600 to 1700 mg l(-1) of L-cysteine and L-cystine from glucos...

journal_title：FEMS microbiology letters

authors： Takagi H,Awano N,Kobayashi S,Noji M,Saito K,Nakamori S

更新日期：1999-10-15 00:00:00

abstract：:A high-level expression system for the Escherichia coli HB101 gamma-glutamyltranspeptidase (GGT) gene was constructed to obtain a sufficient amount of the enzyme for structural studies. About 300 mg of the enzyme was purified from 2 1 of culture broth. Both subunits were needed to reconstitute GGT activity. The E. col...

journal_title：FEMS microbiology letters

authors： Ishiye M,Niwa M

更新日期：1992-10-15 00:00:00

abstract：:Carboxydothermus hydrogenoformans is an extremely thermophilic, Gram-positive bacterium growing on carbon monoxide (CO) as single carbon and energy source and producing only H(2) and CO(2). Carbon monoxide dehydrogenase is a key enzyme for CO metabolism. The carbon monoxide dehydrogenase genes cooF and cooS from C. hy...

journal_title：FEMS microbiology letters

authors： González JM,Robb FT

更新日期：2000-10-15 00:00:00

abstract：:Antimicrobial resistance (AMR) has emerged as one of the most pressing threats to public health. AMR evolution occurs in the clinic but also in the environment, where antibiotics and heavy metals can select and co-select for AMR. While the selective potential of both antibiotics and metals is increasingly well-charact...

journal_title：FEMS microbiology letters

authors： Vos M,Sibleyras L,Lo LK,Hesse E,Gaze W,Klümper U

更新日期：2020-02-01 00:00:00

abstract：:We devised a screening method to obtain basidiomycetous fungi capable of degrading dioxins. About 200 fungal strains were selected from more than 1500 strains by their ability to decolorize Remazol brilliant blue R dye as an indicator. To attempt to eliminate the factor of dioxin sorption by mycelia, we prepared two s...

journal_title：FEMS microbiology letters

authors： Sato A,Watanabe T,Watanabe Y,Harazono K,Fukatsu T

更新日期：2002-08-06 00:00:00

abstract：:The emergence of antibiotic resistance has necessitated new therapeutic approaches for combating persistent bacterial infection. An alternative approach is regulation of bacterial virulence instead of growth suppression, which can readily lead to drug resistance. The virulence of the opportunistic human pathogen Pseud...

journal_title：FEMS microbiology letters

authors： Lee JH,Kim YG,Cho MH,Kim JA,Lee J

更新日期：2012-04-01 00:00:00

abstract：:A characteristic feature of fungal hypha is the presence of large number of nuclei in a common cytoplasmic environment. Where it has been examined, the coenocytic mycelium is commonly heterokaryotic. The nuclei cooperate, compete or combat. It is proposed that in addition to their classical role in heredity, supernume...

journal_title：FEMS microbiology letters

authors： Maheshwari R

更新日期：2005-08-01 00:00:00

abstract：:A red-pigmented, Gram-negative, motile, strictly aerobic, mesophilic, oval- or short rod-shaped bacterium (TDMA-17(T)) was isolated from fresh water collected at Misasa, a radioactive site in Japan. TDMA-17(T) was slightly tolerant against gamma-ray irradiation, and effectively produced carotenoids (2.8 mg g(-1) dry c...

journal_title：FEMS microbiology letters

authors： Asker D,Beppu T,Ueda K

更新日期：2007-08-01 00:00:00

abstract：:Bacteriophages infecting Salmonella spp. were isolated from sewage using soft agar overlays containing three Salmonella serovars and assessed with regard to their potential to control food-borne salmonellae. Two distinct phages, as defined by plaque morphology, structure and host range, were obtained from a single sam...

journal_title：FEMS microbiology letters

authors： Carey-Smith GV,Billington C,Cornelius AJ,Hudson JA,Heinemann JA

更新日期：2006-05-01 00:00:00

abstract：:Reduction of D-fructose to D-mannitol by whole-cell biotransformation with recombinant resting cells of Corynebacterium glutamicum ATCC13032 requires the coexpression of mdh and fdh, which encode mannitol and formate dehydrogenases, respectively. However, d-mannitol formation is limited by the uptake of d-fructose in ...

journal_title：FEMS microbiology letters

authors： Bäumchen C,Krings E,Bringer S,Eggeling L,Sahm H

更新日期：2009-01-01 00:00:00

abstract：:Both form I and II ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) genes were detected in Thiobacillus intermedius by heterologous hybridization using specific probes from Anacystis nidulans and Rhodobacter sphaeroides, respectively. However, only the previously reported form I enzyme could be demonstrated i...

journal_title：FEMS microbiology letters

authors： Stoner MT,Shively JM

更新日期：1993-03-01 00:00:00

abstract：:Type II toxin-antitoxin systems (TAs) are small autoregulated bicistronic operons that encode a toxin protein with the potential to inhibit metabolic processes and an antitoxin protein to neutralize the toxin. Most of the bacterial genomes encode multiple TAs. However, the diversity and accumulation of TAs on bacteria...

journal_title：FEMS microbiology letters

authors： Ramisetty BC,Santhosh RS

更新日期：2016-02-01 00:00:00