Abstract:
:Two cDNAs encoding feedback inhibition-insensitive serine acetyltransferases of Arabidopsis thaliana were expressed in the chromosomal serine acetyltransferase-deficient and L-cysteine non-utilizing Escherichia coli strain JM39-8. The transformants produced 1600 to 1700 mg l(-1) of L-cysteine and L-cystine from glucose. The amount of these amino acids produced per cell was 30 to 60% higher than that of an E. coli strain carrying mutant serine acetyltransferase less sensitive to feedback inhibition.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Takagi H,Awano N,Kobayashi S,Noji M,Saito K,Nakamori Sdoi
10.1111/j.1574-6968.1999.tb08762.xkeywords:
subject
Has Abstractpub_date
1999-10-15 00:00:00pages
453-9issue
2eissn
0378-1097issn
1574-6968pii
S0378109799004292journal_volume
179pub_type
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journal_title:FEMS microbiology letters
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abstract::L-allo-Threonine aldolase (L-allo-threonine acetaldehyde-lyase), which exhibited specificity for L-allo-threonine but not for L-threonine, was purified from a cell-free extract of Aeromonas jandaei DK-39. The purified enzyme catalyzed the aldol cleavage reaction of L-allo-threonine (K(m) = 1.45 mM, Vmax = 45.2 mumol m...
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abstract::The relationship between sugar availability and RTX (repeats in toxin) cytotoxin (leukotoxin) production in the periodontopathic bacterium, Actinobacillus actinomycetemcomitans, was investigated using a chemostat. A actinomycetemcomitans 301-b produced significant amounts of leukotoxin in anaerobic fructose-limited ch...
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journal_title:FEMS microbiology letters
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