Abstract:
:Reduction of D-fructose to D-mannitol by whole-cell biotransformation with recombinant resting cells of Corynebacterium glutamicum ATCC13032 requires the coexpression of mdh and fdh, which encode mannitol and formate dehydrogenases, respectively. However, d-mannitol formation is limited by the uptake of d-fructose in its unphosphorylated form, because additional expression of the sugar facilitator from Zymomonas mobilis resulted in a significantly increased productivity. Here we identified similarities of the myo-inositol transporters IolT1 and IolT2 of C. glutamicum to the sugar facilitator of Z. mobilis. The myo-inositol transporter genes were both individually overexpressed and deleted in recombinants expressing mdh and fdh. Biotransformation experiments showed that the presence and absence, respectively, of IolT1 and IolT2 significantly influenced D-mannitol formation, indicating a D-fructose transport capability of these transporters. For further evidence, a C. glutamicum Delta ptsF mutant unable to grow with D-fructose was complemented with a heterologous fructokinase gene. This resulted in restoration of growth with D-fructose. Using overexpressed iolT1, mdh and fdh, D-mannitol formation obtained with C. glutamicum was 34.2 g L(-1), as opposed to 16 g L(-1) formed by the strain overexpressing only mdh and fdh, showing the suitability of myo-inositol transporters for D-fructose uptake to obtain D-mannitol formation by whole-cell biotransformation with C. glutamicum.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Bäumchen C,Krings E,Bringer S,Eggeling L,Sahm Hdoi
10.1111/j.1574-6968.2008.01425.xsubject
Has Abstractpub_date
2009-01-01 00:00:00pages
227-35issue
2eissn
0378-1097issn
1574-6968pii
FML1425journal_volume
290pub_type
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