Abstract:
:The copZ gene of Bacillus subtilis encodes a copper chaperon CopZ that donates copper to the copper transporter CopA. Both genes copZ and copA are clustered to an operon and its promoter is regulated by Cu ions and CueR, a Mer-like transcriptional activator. Here we show that cadmium ions activate copZA expression as strong as copper ions. Northern hybridization analysis showed that copper and cadmium both induce the synthesis of a 2.7 kb copZA transcript and a 250 bp copZ transcript. A copA deletion mutant was sensitive to copper, whereas a copZ deletion resulted in an increased sensitivity to cadmium and copper. Transcription of the cadmium resistance gene cadA, which is adjacent to the copZA cluster, is extremely reduced in a copZ deletion strain. Transformation of copZ in trans restores wild type resistance to cadmium and copper in a copZ deletion strain. This excludes any polar effect and proves that the copZ encoded protein is important for copper and cadmium resistance.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Solovieva IM,Entian KDdoi
10.1016/j.femsle.2004.05.031keywords:
subject
Has Abstractpub_date
2004-07-01 00:00:00pages
115-22issue
1eissn
0378-1097issn
1574-6968pii
S0378109704003696journal_volume
236pub_type
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