Metalloregulation in Bacillus subtilis: the copZ chromosomal gene is involved in cadmium resistance.

Abstract:

:The copZ gene of Bacillus subtilis encodes a copper chaperon CopZ that donates copper to the copper transporter CopA. Both genes copZ and copA are clustered to an operon and its promoter is regulated by Cu ions and CueR, a Mer-like transcriptional activator. Here we show that cadmium ions activate copZA expression as strong as copper ions. Northern hybridization analysis showed that copper and cadmium both induce the synthesis of a 2.7 kb copZA transcript and a 250 bp copZ transcript. A copA deletion mutant was sensitive to copper, whereas a copZ deletion resulted in an increased sensitivity to cadmium and copper. Transcription of the cadmium resistance gene cadA, which is adjacent to the copZA cluster, is extremely reduced in a copZ deletion strain. Transformation of copZ in trans restores wild type resistance to cadmium and copper in a copZ deletion strain. This excludes any polar effect and proves that the copZ encoded protein is important for copper and cadmium resistance.

journal_name

FEMS Microbiol Lett

authors

Solovieva IM,Entian KD

doi

10.1016/j.femsle.2004.05.031

keywords:

subject

Has Abstract

pub_date

2004-07-01 00:00:00

pages

115-22

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378109704003696

journal_volume

236

pub_type

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