Surface Listeria monocytogenes carbohydrate-binding components revealed by agglutination with neoglycoproteins.

abstract：:We have constructed a Thermus promoter probe vector pPP11 containing the promoterless heat-stable kanamycin resistance (Kmr) gene. Total DNA of T. thermophilus HB27 was randomly sheared into 100-200 bp fragments and inserted into the EcoRI site of pPP11. About 1000 clones which showed Kmr were obtained, and they were ...

journal_title：FEMS microbiology letters

authors： Maseda H,Hoshino T

更新日期：1995-05-01 00:00:00

abstract：:Himalaya hosts a unique environment for microbial ecology. The present study aimed to explore the bioplastic producing bacterial communities along altitude gradient of Pangi-Chamba trans-Himalayan region. A total of 411 bacteria were isolated and 70 characterized at the molecular level for potential polyhydroxyalkanoa...

journal_title：FEMS microbiology letters

authors： Kumar V,Thakur V,Ambika,Kumar S,Singh D

更新日期：2018-07-01 00:00:00

abstract：:The Great Salt Plains is a 65-km(2) hypersaline habitat of geological origin located in north-central Oklahoma. Contamination of such ecosystems by petroleum compounds is expected from non-point sources and due to increased human activities. Little information exists about the ability of halophilic and halotolerant ba...

journal_title：FEMS microbiology letters

authors： Nicholson CA,Fathepure BZ

更新日期：2005-04-15 00:00:00

abstract：:Total dsRNA extractions in five killer K2 strains of Saccharomyces cerevisiae isolated from spontaneous fermentations revealed the presence of a novel dsRNA fragment (which we named NS dsRNA) of approximately 1.30 kb, together with L and M2 dsRNAs. NS dsRNA appeared to be encapsidated in the same kind of viral particl...

journal_title：FEMS microbiology letters

authors： Cansado J,Barros Velázquez J,Sieiro C,Gacto M,Villa TG

更新日期：1999-12-15 00:00:00

abstract：:Nitrogen is a key nutrient for land plants and phytoplankton in terrestrial and aquatic ecosystems. The model alga Chlamydomonas reinhardtii can grow efficiently on several inorganic nitrogen sources (e.g. ammonium, nitrate, nitrite) as well as many amino acids. In this study, we show that Chlamydomonas is unable to u...

journal_title：FEMS microbiology letters

authors： Calatrava V,Hom EFY,Llamas Á,Fernández E,Galván A

更新日期：2018-04-01 00:00:00

abstract：:In this analysis of membrane lipid compositions in Helicobacter pylori, the membrane lipid profiles drastically changed during coccoid formation: cholesteryl-6-O-tetradecanoyl-alpha-D-glucopyranoside levels increased, cholesteryl-alpha-D-glucopyranoside and phosphatidyl ethanolamine decreased, and a coordinated increa...

journal_title：FEMS microbiology letters

authors： Shimomura H,Hayashi S,Yokota K,Oguma K,Hirai Y

更新日期：2004-08-15 00:00:00

abstract：:The increase in Antimicrobial resistance (AMR) microorganisms has been exacerbated by exposure to antimicrobial drugs (e.g. antibiotics). A solution to AMR may require academic researchers to not only contribute to the drug discovery pipeline through laboratory research, but also to engage and inform non-specialist au...

journal_title：FEMS microbiology letters

authors： Redfern J,Bowater L,Crossley M,Verran J

更新日期：2018-08-01 00:00:00

abstract：:Recent reports have indicated that cholesterol plays a crucial role during the uptake of mycobacteria by macrophages. However, the significance of cholesterol modification enzymes encoded by Mycobacterium tuberculosis for bacterial pathogenicity remains unknown. Here, the authors explored whether the well-known choles...

journal_title：FEMS microbiology letters

authors： Brzostek A,Dziadek B,Rumijowska-Galewicz A,Pawelczyk J,Dziadek J

更新日期：2007-10-01 00:00:00

abstract：:The production of candidate affinity proteins in a soluble form, for downstream characterization, is often a time-consuming step in combinatorial protein engineering methods. Here, a novel approach for efficient production of candidate clones is described based on direct cleavage of the affinity protein from the surfa...

journal_title：FEMS microbiology letters

authors： Kronqvist N,Löfblom J,Severa D,Ståhl S,Wernérus H

更新日期：2008-01-01 00:00:00

abstract：:To investigate the regulatory mechanism governing antifungal metabolite biosynthesis, two kinds of global regulator genes in Pseudomonas sp. M18, an rpoS and an rsmA gene, were cloned and mutated by inserting with an aacC1 cassette, respectively. Two mutants showed the same regulatory mode with repression of phenazine...

journal_title：FEMS microbiology letters

authors： Ge Y,Yang S,Fang Y,Yang R,Mou D,Cui J,Wen L

更新日期：2007-03-01 00:00:00

abstract：:We have constructed a very efficient synthesis and secretion system for cholera toxin B subunit (CTB) of Vibrio cholerae 569B using Bacillus-brevis. The constructed expression-secretion vector has the multiple promoters and the signal peptide coding region of the mwp gene, a structural gene for one of the major cell w...

journal_title：FEMS microbiology letters

authors： Ichikawa Y,Yamagata H,Tochikubo K,Udaka S

更新日期：1993-08-01 00:00:00

abstract：:Mixed-substrate conversions are an under-regarded option to fix carbon dioxide in significant amounts. In such a conversion, carbon dioxide together with one other carbon source such as glucose is converted to a single carbon product. With mixed-substrate conversions, it is possible to incorporate carbon dioxide into ...

journal_title：FEMS microbiology letters

authors： Steiger MG,Mattanovich D,Sauer M

更新日期：2017-11-15 00:00:00

abstract：:Biotype, genome, protein and plasmid profile diversity amongst 40 epidemiologically unrelated strains of Helicobacter pylori was studied. Strains were API Zym biotypes II, III and IV but most (87%) were biotype II. Four subsets of strains were defined on a combination of motility (56% positive) and cytotoxin productio...

journal_title：FEMS microbiology letters

authors： Owen RJ,Bickley J,Moreno M,Costas M,Morgan DR

更新日期：1991-04-15 00:00:00

abstract：:Cry4Ba is a delta-endotoxin produced by Bacillus thuringiensis subsp. israelensis and Cyt2Aa2 is a cytolytic delta-endotoxin produced by B. thuringiensis subsp. darmstadiensis. Cry4Ba produced in Escherichia coli was toxic to Aedes aegypti larvae (LC(50)=140 ng ml(-1)) but virtually inactive to Culex quinquefasciatus ...

journal_title：FEMS microbiology letters

authors： Promdonkoy B,Promdonkoy P,Panyim S

更新日期：2005-11-01 00:00:00

abstract：:The purpose of this study was to investigate the feasibility of cultivating the biotechnologically important bacterium Streptomyces griseus in single-species and mixed-species biofilms using a tubular biofilm reactor (TBR). Streptomyces griseus biofilm development was found to be cyclical, starting with the initial ad...

journal_title：FEMS microbiology letters

authors： Winn M,Casey E,Habimana O,Murphy CD

更新日期：2014-03-01 00:00:00

abstract：:A Gram-stain-negative bacterial strain, JBTF-M27T, was isolated from a tidal flat from Yellow Sea, Republic of Korea. Neighbor-joining phylogenetic tree of 16S rRNA gene sequences showed that strain JBTF-M27T fell within the clade comprising the type strains of Sulfitobacter species. Strain JBTF-M27T exhibited the hig...

journal_title：FEMS microbiology letters

authors： Park S,Chen S,Lee JS,Kim W,Yoon JH

更新日期：2020-05-01 00:00:00

abstract：:Three heterocytous cyanobacterial strains were isolated from different habitats of Central India, and initial morphological studies indicated them to be members of the genus Nostoc or other closely related genera. Subsequent studies using morphological, ecological, molecular and phylogenetic methods indicated the thre...

journal_title：FEMS microbiology letters

authors： Saraf A,Dawda HG,Suradkar A,Behere I,Kotulkar M,Shaikh ZM,Kumat A,Batule P,Mishra D,Singh P

更新日期：2018-12-01 00:00:00

abstract：:The regulation of nitrogen assimilation was investigated in the Gram-positive actinomycete Corynebacterium glutamicum. Biochemical studies and site-directed mutagenesis revealed that glutamine synthetase activity is regulated via adenylylation in this organism. The genes encoding the central signal transduction protei...

journal_title：FEMS microbiology letters

authors： Jakoby M,Krämer R,Burkovski A

更新日期：1999-04-15 00:00:00

abstract：:Linkage of rumen microbial structure to host phenotypical traits may enhance the understanding of host-microbial interactions in livestock species. This study used culture-independent PCR-denaturing gradient gel electrophoresis (PCR-DGGE) to investigate the microbial profiles in the rumen of cattle differing in feed e...

journal_title：FEMS microbiology letters

authors： Guan LL,Nkrumah JD,Basarab JA,Moore SS

更新日期：2008-11-01 00:00:00

abstract：:Chromohalobacter marismortui VH1 was screened for its ability to utilise organosulfonate compounds at a range of NaCl concentrations. Only aminomethane sulfonate, of seven sulfonates tested, was utilised. Length of lag phase during growth on aminomethane sulfonate, as either nitrogen and/or sulfur source, increased wi...

journal_title：FEMS microbiology letters

authors： Ternan NG,McMullan G

更新日期：2002-01-22 00:00:00

abstract：:Bacillus anthracis can be identified on the basis of the detection of virulence factor genes located on two plasmids, pXO1 and pXO2. Thus isolates lacking both pXO1 and pXO2 are indistinguishable from closely related B. cereus group bacteria. We developed a multiplex PCR assay for characterization of B. anthracis isol...

journal_title：FEMS microbiology letters

authors： Ramisse V,Patra G,Garrigue H,Guesdon JL,Mock M

更新日期：1996-11-15 00:00:00

abstract：:Cytochemical staining and microscopy were used to study the trophic structures and cellular morphotypes that are produced during the colonization of oil-water interfaces by oil-degrading yeasts and bacteria. Among the microorganisms studied here, the yeasts (Schwanniomyces occidentalis, Torulopsis candida, Candida tro...

journal_title：FEMS microbiology letters

authors： Dmitriev VV,Crowley D,Rogachevsky VV,Negri CM,Rusakova TG,Kolesnikova SA,Akhmetov LI

更新日期：2011-02-01 00:00:00

abstract：:Sixteen of the 18 Vibrio cholerae rough strains isolated from hospitalised diarrhoea patients were found to contain O1 serotype-specific (wbe) genes and all currently known virulence genes. Expression of the regulatory element ToxR was evident in these strains. Cholera toxin production ability of the rough strains was...

journal_title：FEMS microbiology letters

authors： De K,Ramamurthy T,Faruque SM,Yamasaki S,Takeda Y,Nair GB,Nandy RK

更新日期：2004-03-12 00:00:00

abstract：:A physical and functional map of Aspergillus tubingensis mtDNA type 2b was constructed and compared to Aspergillus niger typeI a mtDNA. The gene content and order, as well as the patterns of restriction sites, were similar. Two unidentified ORFs and several repeat elements were found in the region between the ndhl and...

journal_title：FEMS microbiology letters

authors： Juhász A,Láday M,Gácser A,Kucsera J,Pfeiffer I,Kevei F,Hamari Z

更新日期：2004-12-01 00:00:00

abstract：:CRISPR-Cas systems provide adaptive immunity against mobile genetic elements, but employment of this resistance mechanism is often reported with a fitness cost for the host. Whether or not CRISPR-Cas systems are important barriers for the horizontal spread of conjugative plasmids, which play a crucial role in the spre...

journal_title：FEMS microbiology letters

authors： van Sluijs L,van Houte S,van der Oost J,Brouns SJ,Buckling A,Westra ER

更新日期：2019-03-01 00:00:00

abstract：:In order to investigate whether the iron acquisition mechanisms of Staphylococcus aureus are induced by iron restriction in vitro, we examined S. aureus ATCC 6538 for production of siderophore and expression of transferrin-binding protein (SA-tbp) in normal or deferrated brain heart infusion broth (BHI). Siderophore p...

journal_title：FEMS microbiology letters

authors： Lim Y,Shin SH,Lee SI,Kim IS,Rhee JH

更新日期：1998-06-01 00:00:00

abstract：:The Escherichia coli yehZYXW operon encodes a putative osmoprotectant uptake system of the ABC transporter family. yehZ is identical to osmF, an osmotically inducible gene identified previously. Construction and analysis of a yehZ-lacZ transcriptional fusion demonstrated that yehZ is inducible not only by osmolarity, ...

journal_title：FEMS microbiology letters

authors： Checroun C,Gutierrez C

更新日期：2004-07-15 00:00:00

abstract：:Currently, interest in phage science is on the rise again as this subject remains largely unexplored, and its potential diversity mainly untapped. Here, we present phage vB_BsuP-Goe1, a new isolate that infects Bacillus subtilis The phage forms round plaques with a matt outline on agar plates containing a B. subtilis ...

journal_title：FEMS microbiology letters

authors： Willms IM,Hertel R

更新日期：2016-10-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic pathogen that can cause life-threatening infections in patients admitted to intensive care units. Resistance rapidly develops against two drugs of choice: ceftazidime and meropenem. Several therapeutic protocols were compared for reduction in viable cells and limiting develop...

journal_title：FEMS microbiology letters

authors： Feng Y,Bakker RT,van Hest RM,Hodiamont CJ,Brul S,Schultsz C,Ter Kuile BH

更新日期：2017-08-01 00:00:00

abstract：:The insertion sequence ISRm8 was identified by sequence analysis of the cryptic plasmid pRmeGR4b of Sinorhizobium meliloti GR4. ISRm8 is 1451 bp in length and carries 22/24-bp terminal imperfect inverted repeats with seven mismatches and a direct target site duplication of 3 bp. ISRm8 carries a unique open reading fra...

journal_title：FEMS microbiology letters

authors： Zekri S,Toro N

更新日期：1998-01-01 00:00:00