Abstract:
:Mycoplasma agalactiae is a worldwide ruminant pathogen that causes significant economic losses by inflicting contagious agalactia in sheep and goats. The development of efficient control strategies requires a better understanding of the mycoplasma factors that promote successful infection. However, lack of genetic tools has been a major impediment in studying the pathogenic mechanisms of M. agalactiae. This study describes the identification and cloning of the M. agalactiae origin of replication (oriC) in order to construct the first shuttle vectors for targeted gene disruption, gene complementation and expression studies. Additionally, this report provides the first evidence of the occurrence of homologous recombination and the functionality of heterologous tetM determinant in this pathogen.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Chopra-Dewasthaly R,Marenda M,Rosengarten R,Jechlinger W,Citti Cdoi
10.1016/j.femsle.2005.09.021keywords:
subject
Has Abstractpub_date
2005-12-01 00:00:00pages
89-94issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(05)00656-7journal_volume
253pub_type
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