Abstract:
:A 3.5-kb native plasmid (pND103) was identified in Streptococcus thermophilus ST2-1. Preliminary sequence analysis indicated that pND103 belongs to group I S. thermophilus plasmids. A region of approximately 2 kb appears to contain three components: a plus origin of replication (ori) typical of plasmids that replicate via rolling circle replication; a gene encoding a replication protein (rep); and a gene encoding a small heat shock protein (hsp). pND103 was then used to construct S. thermophilus/Escherichia coli hybrid cloning vectors by ligating different portions of pND103 to an origin-probe vector (pND330) composed of pUC19 and a Gram-positive erythromycin resistance gene. The shuttle vectors (pND913, pND914 and pND915) were successfully introduced back into plasmid-free S. thermophilus ST3-1 as well as to Lactococcus lactis LM0230 and E. coli JM109. Segregational and structural stability study indicated that these vectors can be maintained in these hosts. The results indicated that pND913, pND914 and pND915 are potential shuttle cloning vectors for S. thermophilus.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Su P,Jury K,Allison GE,Wong WY,Kim WS,Liu CQ,Vancov T,Dunn NWdoi
10.1111/j.1574-6968.2002.tb11412.xkeywords:
subject
Has Abstractpub_date
2002-10-29 00:00:00pages
43-7issue
1eissn
0378-1097issn
1574-6968pii
S0378109702009709journal_volume
216pub_type
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