Construction of improved plasmid vectors for promoter characterization in Pseudomonas aeruginosa and other gram-negative bacteria.

Abstract:

:We report the construction of two broad host range promoter-probe plasmid vectors for rapid analysis of promoters in Gram-negative bacteria. The new vectors, pME4507 and pME4510, carry carbenicillin and gentamycin resistance genes, respectively, and are small sized (4 kb) with a flexible multiple cloning site to facilitate directional cloning of putative promoter elements. The vectors allow rapid plate-based screening for promoter activities, using beta-galactosidase as the reporter enzyme. In the absence of an inserted promoter fragment, they display very low background activity, making them a useful tool for analysis of low expression level promoters.

journal_name

FEMS Microbiol Lett

authors

Rist M,Kertesz MA

doi

10.1111/j.1574-6968.1998.tb13315.x

subject

Has Abstract

pub_date

1998-12-01 00:00:00

pages

179-83

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(98)00481-9

journal_volume

169

pub_type

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