Genomic heterogeneity in Chlorobium limicola: chromosomic and plasmidic differences among strains.


:Chromosome analysis by pulsed-field gel electrophoresis shows a high level of genetic heterogeneity between the two subspecies of the green sulfur bacteria Chlorobium limicola analysed: C. limicola and C. limicola f. s. thiosulfatophilum. Currently, they are differentiated only by the ability to utilize thiosulfate as photosynthetic electron donor, and by their %G + C content (51% and 58.1%, respectively). However, the capacity to utilize thiosulfate as photosynthetic electron donor does not appear to be a useful criterion to differentiate between some strains of this species, because this ability is encoded by plasmids that are different depending on the thiosulfatophilum strain analysed. In contrast, this study reveals that the comparison of chromosomal restriction patterns is very useful as an additional aid for the differentiation and identification of C. limicola strains.


FEMS Microbiol Lett


Méndez-Alvarez S,Pavoń V,Esteve I,Guerrero R,Gaju N




Has Abstract


1995-12-15 00:00:00












  • The complex and dynamic genomes of industrial yeasts.

    abstract::The Saccharomyces sensu stricto genus contains many species that are industrially important for fermentation of wines, beers and ales. The molecular characterization of the genomes of yeasts involved in these processes reveals that the majority arose from interspecific hybridization between two and sometimes three yea...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Querol A,Bond U

    更新日期:2009-04-01 00:00:00

  • Characterization of a novel plasmid from extremely halophilic Archaea: nucleotide sequence and function analysis.

    abstract::We determined the complete nucleotide sequence of the 16341 bp plasmid pHH205 of the extremely halophilic archaeon Halobacterium salinarum J7. The plasmid has a G+C content of 61.1%. A number of direct and inverted repeat sequences were found in pHH205, while no insertion sequences were found. Thirty-eight large open ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ye X,Ou J,Ni L,Shi W,Shen P

    更新日期:2003-04-11 00:00:00

  • Genomic suppression subtractive hybridization as a tool to identify differences in mycorrhizal fungal genomes.

    abstract::Characterization of genomic variation among different microbial species, or different strains of the same species, is a field of significant interest with a wide range of potential applications. We have investigated the genomic variation in mycorrhizal fungal genomes through genomic suppressive subtractive hybridizati...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Murat C,Zampieri E,Vallino M,Daghino S,Perotto S,Bonfante P

    更新日期:2011-05-01 00:00:00

  • Characterization of the eaeA gene from rabbit enteropathogenic Escherichia coli strain RDEC-1 and comparison to other eaeA genes from bacteria that cause attaching-effacing lesions.

    abstract::A number of enteric pathogens, including enteropathogenic (EPEC) and enterohemorrhagic (EHEC) Escherichia coli, Hafnia alvei, a strain of Citrobacter freundii, and rabbit EPEC strain RDEC-1 cause attaching-effacing (AE) lesions in the gut mucosa. These bacteria have a pathogenicity cassette (locus of enterocyte efface...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Agin TS,Cantey JR,Boedeker EC,Wolf MK

    更新日期:1996-11-01 00:00:00

  • Supersusceptibility to hydrophobic antimicrobial agents and cell surface hydrophobicity in Branhamella catarrhalis.

    abstract::To clarify the cause of the supersusceptibility of Branhamella catarrhalis to macrolide antibiotics, which are well-known to be inactive to most Gram-negative bacteria, we determined its cell surface hydrophobicity by the partition experiment between water and hydrocarbons. Its cell surface was found to be markedly mo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gotoh N,Tanaka S,Nishino T

    更新日期:1989-05-01 00:00:00

  • Biotype and macromolecular profiles of cytotoxin-producing strains of Helicobacter pylori from antral gastric mucosa.

    abstract::Biotype, genome, protein and plasmid profile diversity amongst 40 epidemiologically unrelated strains of Helicobacter pylori was studied. Strains were API Zym biotypes II, III and IV but most (87%) were biotype II. Four subsets of strains were defined on a combination of motility (56% positive) and cytotoxin productio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Owen RJ,Bickley J,Moreno M,Costas M,Morgan DR

    更新日期:1991-04-15 00:00:00

  • Isolation of genes encoding beta-D-xylanase, beta-D-xylosidase and alpha-L-arabinofuranosidase activities from the rumen bacterium Prevotella ruminicola B1(4).

    abstract::Prevotella ruminicola B1(4) is a strictly anaerobic, Gram-negative, polysaccharide-degrading rumen bacterium. Xylanase activity in this strain was found to be inducible, the specific activity of cells grown on xylan being increased at least 20-fold by comparison with cells grown on glucose. Ten bacteriophage clones ex...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gasparic A,Marinsek-Logar R,Martin J,Wallace RJ,Nekrep FV,Flint HJ

    更新日期:1995-01-15 00:00:00

  • Efficient transfer of antibiotic resistance plasmids by transduction within methicillin-resistant Staphylococcus aureus USA300 clone.

    abstract::The epidemic community-associated methicillin-resistant clone Staphylococcus aureus USA300 is a major source of skin and soft tissue infections and involves strains with a diverse set of resistance genes. In this study, we report efficient transduction of penicillinase and tetracycline resistance plasmids by bacteriop...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Varga M,Kuntová L,Pantůček R,Mašlaňová I,Růžičková V,Doškař J

    更新日期:2012-07-01 00:00:00

  • Phylogeographic relationships in the polypore fungus Pycnoporus inferred from molecular data.

    abstract::The genus Pycnoporus forms a group of four species known especially for producing high redox potential laccases suitable for white biotechnology. A sample of 36 Pycnoporus strains originating from different geographical areas was studied to seek informative molecular markers for the typing of new strains in laboratory...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lesage-Meessen L,Haon M,Uzan E,Levasseur A,Piumi F,Navarro D,Taussac S,Favel A,Lomascolo A

    更新日期:2011-12-01 00:00:00

  • EnvC, a new lipoprotein of the cytoplasmic membrane of Escherichia coli.

    abstract::A gene product with an apparent molecular mass of approximately 39,000 Da can be identified in the cytoplasmic membrane of Escherichia coli upon expression of cloned envC. In this communication we report that the product was labelled with [3H]glycerol and [3H]palmitic acid, and a precursor molecule of increased molecu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Seiffer D,Klein JR,Plapp R

    更新日期:1993-03-01 00:00:00

  • The discriminatory power of MALDI-TOF mass spectrometry to differentiate between isogenic teicoplanin-susceptible and teicoplanin-resistant strains of methicillin-resistant Staphylococcus aureus.

    abstract::To explore the discriminatory power of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for detecting subtle differences in isogenic isolates, we tested isogenic strains of Staphylococcus aureus differing in their expression of resistance to methicillin or teicoplanin. More i...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Majcherczyk PA,McKenna T,Moreillon P,Vaudaux P

    更新日期:2006-02-01 00:00:00

  • Construction and physiological analysis of a Xanthomonas oryzae pv. oryzae recA mutant.

    abstract::A Xoo recA insertion inactivation mutant was constructed. The mutant, lacking RecA, showed increased sensitivity towards mutagen killing. This phenotype could be complemented by a cloned, functional recA. Unlike other bacteria, both the recA mutant and the parental strain had similar level of resistance to H2O2 killin...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Mongkolsuk S,Rabibhadana S,Sukchavalit R,Vaughn G

    更新日期:1998-12-15 00:00:00

  • Conversion of methionine to methional by Lactococcus lactis.

    abstract::Lactic acid bacteria were screened for methional production from 4-methylthio-2-ketobutanoate. Only Lactococcus lactis IFPL730 produced high amounts of methional. It was demonstrated that production of this compound was an exclusively enzymatic reaction. The present work describes for the first time that L. lactis can...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Amárita F,Fernández-Esplá D,Requena T,Pelaez C

    更新日期:2001-10-16 00:00:00

  • Distribution of insertion sequence IS1 in multiple-antibiotic resistant clinical Enterobacteriaceae strains.

    abstract::The presence of insertion sequence IS1 in 70 multiple-antibiotic resistant clinical strains was determined. This 70-strain collection comprised 46 Escherichia coli, 18 Salmonella and 6 Shigella strains. The presence of IS1 was detected in the chromosome and plasmids of 73% and 63% of the strains, respectively, and 51%...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ramirez-Santos J,Alvarez G,Cisneros E,Gomez-Eichelmann MC

    更新日期:1992-06-01 00:00:00

  • From bioremediation to biowarfare: on the impact and mechanism of type IV secretion systems.

    abstract::Type IV secretion systems are employed by a wide variety of Gram-negative microorganisms for the translocation of macromolecules across the cell envelope. The translocated substrates (proteins, protein-DNA complexes and DNA) are as diverse as the organisms on the donor and recipient side of the translocation process. ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Baron C

    更新日期:2005-12-15 00:00:00

  • Evidence that OmcB and OmpB of Geobacter sulfurreducens are outer membrane surface proteins.

    abstract::The c-type cytochrome (OmcB) and the multicopper protein (OmpB) required for Fe(III) oxide reduction by Geobacter sulfurreducens were predicted previously to be outer membrane proteins, but it is not clear whether they are positioned in a manner that permits the interaction with Fe(III). Treatment of whole cells with ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Qian X,Reguera G,Mester T,Lovley DR

    更新日期:2007-12-01 00:00:00

  • Variation and predicted structure of the flagellin gene in Actinoplanes species.

    abstract::Members of the genus Actinoplanes are considered to be representative of motile actinomycetes. To infer the flagellar diversity of Actinoplanes species, novel degenerate primers were designed for the flagellin (fliC) gene. The fliC gene of 21 Actinoplanes strains was successfully amplified and classified into two grou...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Yamamura H,Hanawa K,Kusunoki M,Nakamura K,Nakagawa Y,Ishida Y,Hamada M,Otoguro M,Tamura T,Fujita N,Hayakawa M

    更新日期:2011-11-01 00:00:00

  • Evaluation of nutritional and physical stress conditions during vegetative growth on conidial production and germination in Ophiocordyceps sinensis.

    abstract::The influence of nutritional and physical stress on sporulation, conidial germination and vegetative biomass of Ophiocordyceps sinensis, one of the most important medicinal fungi in China and now globally, was evaluated using a two-stage culture method. All the treatments, except nutrient deprivation, enhanced conidia...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Ren SY,Yao YJ

    更新日期:2013-09-01 00:00:00

  • Growth and morphogenesis in Streptomyces.

    abstract::In this report we propose a model of apical growth for streptomycetes. The apical tip is considered as a multilayered wall that expands by an inside-to-outside mechanism of growth. It is also assumed that each layer is made up of peptidoglycan blocks, each of them being the result of the biosynthetic activity of a wal...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Miguélez EM,Martín C,Manzanal MB,Hardisson C

    更新日期:1992-12-15 00:00:00

  • Cloning and analysis of the polyhydroxyalkanoic acid synthase gene from an Acinetobacter sp.: evidence that the gene is both plasmid and chromosomally located.

    abstract::The polyhydroxyalkanoic acid (PHA) synthase gene (phaCAc) of a species of Acinetobacter isolated from an activated sludge treatment plant was cloned by heterologous complementation in a poly-beta-hydroxybutyrate (PHB) negative mutant of Alcaligenes eutrophus. Nucleotide sequence analysis of phaCAc revealed an open rea...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Schembri MA,Bayly RC,Davies JK

    更新日期:1994-05-01 00:00:00

  • Importance of fumarate and nitrate reduction regulatory protein for intestinal proliferation of Vibrio vulnificus.

    abstract::The sepsis caused by Vibrio vulnificus is characterized by an average incubation period of 26 h and a high mortality rate exceeding 50%. The fast growth and dissemination of V. vulnificus in vivo lead to poor clinical outcomes in patients. Therefore, elucidation of the proliferation mechanisms of this organism in vivo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kado T,Kashimoto T,Yamazaki K,Ueno S

    更新日期:2017-01-01 00:00:00

  • Nested PCR for the detection of Candidatus arthromitus in fish.

    abstract::Rainbow trout gastroenteritis has been related to the accumulation of segmented filamentous bacteria in the digestive tract of fish, which presents lethargy, reduced appetite and accumulation of mucoid faeces. Some authors associate the comparison of illness with the presence of viable filaments, which produce and rel...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Manzano M,Giusto C,Iacumin L,Patthey C,Cecchini F,Fontanillas R,Comi G

    更新日期:2010-07-01 00:00:00

  • Disruption of the mycobacterial cell entry gene of Mycobacterium bovis BCG results in a mutant that exhibits a reduced invasiveness for epithelial cells.

    abstract::Mycobacteria belonging to the Mycobacterium tuberculosis complex have the ability to invade and replicate in non-phagocytic cells, an event that requires the presence of bacterial surface components capable of triggering a cell response and the subsequent internalization of the microorganism. In this study, we report ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Flesselles B,Anand NN,Remani J,Loosmore SM,Klein MH

    更新日期:1999-08-15 00:00:00

  • Yersinia pestis lacZ expresses a beta-galactosidase with low enzymatic activity.

    abstract::Although very little, if any, beta-galactosidase activity is detected in Yersinia pestis by a standard Miller assay, we found that Y. pestis KIM6+ cells formed blue colonies on plates containing 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal). Searches of the Y. pestis genome databases revealed the presence of n...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Bobrov AG,Perry RD

    更新日期:2006-02-01 00:00:00

  • Comparison of the thickening properties of four Lactobacillus delbrueckii subsp. bulgaricus strains and physicochemical characterization of their exopolysaccharides.

    abstract::It is now well established that physicochemical properties of exopolysaccharides (EPS) can vary between strains of a given species and according to growth conditions. The EPS production of four strains of Lactobacillus bulgaricus was monitored during growth in milk and in a chemically defined media. All strains, inclu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Petry S,Furlan S,Waghorne E,Saulnier L,Cerning dagger J,Maguin E

    更新日期:2003-04-25 00:00:00

  • Distribution of the IS elements ISS1 and IS904 in lactococci.

    abstract::A broad distribution of the lactococcal IS elements ISS1 [1] and IS904 [2] in several lactococcal plasmids and chromosomal DNA was observed. Hybridization of the ISS1 and IS904 oligonucleotide gene probes with DNA of lactococcal phages showed that none of these tested bacteriophages contained one of the IS elements. O...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Schäfer A,Jahns A,Geis A,Teuber M

    更新日期:1991-05-15 00:00:00

  • A structurally novel staphylococcal protein A from the V8 strain.

    abstract::Protein A from the Staphylococcus aureus strain V8 has a molecular mass about 8000 Da less than that of known proteins A. The corresponding gene was cloned and expressed in Escherichia coli. Sequence analysis of this structurally new protein A revealed that it lacked an IgG-binding domain (58 amino acids), and that it...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Finck-Barbançon V,Prevost G,Mazurier I,Piemont Y

    更新日期:1992-02-01 00:00:00

  • Phylogenetic analysis of polycyclic aromatic hydrocarbon degrading mycobacteria by 16S rRNA sequencing.

    abstract::Mycobacterium sp. PYR-1 was previously isolated in our laboratory and was shown to be able to mineralize high molecular mass polycyclic aromatic hydrocarbons (PAHs) [Heitkamp and Cerniglia, (1988) Appl. Environ. Microbiol. 54, 1612-1614]. In this research, the 16S rRNA gene (rDNA) of this strain was amplified by polym...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wang RF,Cao WW,Cerniglia CE

    更新日期:1995-07-15 00:00:00

  • Effect of antibiotics on the bacterial population of the rabbit caecum.

    abstract::The effect feeding antibiotics has on the bacterial population of the rabbit caecum was investigated. No changes in total volatile fatty acid production or total bacterial counts were observed compared with nonantibiotic treated controls. However, treatment with chlortetracycline resulted in an increase of propionate ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Abecia L,Fondevila M,Balcells J,Edwards JE,Newbold CJ,McEwan NR

    更新日期:2007-07-01 00:00:00

  • Regulatory role of pyruvate-sensing BtsSR in biofilm formation by Escherichia coli K-12.

    abstract::Pyruvate, the key regulator in connection of a variety of metabolic pathways, influences transcription of the Escherichia coli genome through controlling the activity of two pyruvate-sensing two-component systems (TCSs), BtsSR and PyrSR. Previously, we identified the whole set of regulatory targets of PyrSR with low-a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ogasawara H,Ishizuka T,Yamaji K,Kato Y,Shimada T,Ishihama A

    更新日期:2019-12-01 00:00:00