Isolation and characterization of a cytotoxin produced by Plesiomonas shigelloides P-1 strain.

abstract：:The galE gene of Pasteurella multocida has been isolated by complementing galE-defective mutants of Salmonella typhimurium with a plasmid library of this organism. The complete nucleotide sequence of the P. multocida galE gene consists of 1017 nucleotides, encoding a predicted polypeptide of 339 amino acids. The deduc...

journal_title：FEMS microbiology letters

authors： Fernández de Henestrosa AR,Badiola I,Saco M,Perez de Rozas AM,Campoy S,Barbé J

更新日期：1997-09-15 00:00:00

abstract：:The nitrogen-fixing cyanobacterium Nostoc sp. ATCC 29132 was shown to contain two sequence-specific endonucleases. Nsp(29132) I was an isoschizomer of AsuII, and Nsp(29132) II was an isoschizomer of BamHI. Nsp(29132) II was shown to generate ends that could be ligated to those generated by BamHI. ...

journal_title：FEMS microbiology letters

authors： Muro-Pastor AM,Herrero A,Flores E

更新日期：1991-01-01 00:00:00

abstract：:The cellulase-producing fungal strain Y-94, isolated in Japan and invalidly described as Acremonium cellulolyticus nom. nud. strain Y-94, seldom forms enteroarthric conidia under nutrient starvation conditions. Phylogenetic analysis using ITS1-5.8S-ITS2 and RNA polymerase II large subunit gene sequences revealed that ...

journal_title：FEMS microbiology letters

authors： Fujii T,Hoshino T,Inoue H,Yano S

更新日期：2014-02-01 00:00:00

abstract：:Thermophilic bacteria have recently attracted great attention because of their potential application in improving different biochemical processes such as anaerobic digestion of various substrates, wastewater treatment or hydrogen production. In this study we report on the design of a specific 16S rRNA-targeted oligonu...

journal_title：FEMS microbiology letters

authors： Gagliano MC,Braguglia CM,Rossetti S

更新日期：2014-09-01 00:00:00

abstract：:Enterococcus faecium has emerged as an important cause of nosocomial infections over the last two decades. We recently demonstrated collagen type I (CI) as a common adherence target for some E. faecium isolates and a significant correlation was found to exist between acm-mediated CI adherence and clinical origin. Here...

journal_title：FEMS microbiology letters

authors： Zhao M,Sillanpää J,Nallapareddy SR,Murray BE

更新日期：2009-11-01 00:00:00

abstract：:We recently identified the phaG(Pp) gene encoding (R)-3-hydroxydecanoyl-ACP:CoA transacylase in Pseudomonas putida, which directly links the fatty acid de novo biosynthesis and polyhydroxyalkanoate (PHA) biosynthesis. An open reading frame (ORF) of which the deduced amino acid sequence shared about 57% identity with P...

journal_title：FEMS microbiology letters

authors： Hoffmann N,Steinbüchel A,Rehm BH

更新日期：2000-03-15 00:00:00

abstract：:pSA1.1 is a 9.1-kb multicopy plasmid originally isolated from Streptomyces cyaneus (formerly S. azureus) ATCC 14921. This plasmid accumulates single-stranded DNA in S. lividans and is therefore considered to replicate by a rolling-circle replication. In the present work, the rep gene encoding the replication initiator...

journal_title：FEMS microbiology letters

authors： Yokoyama E,Matsuzaki Y,Doi K,Ogata S

更新日期：1998-12-01 00:00:00

abstract：:Strains of Salmonella enteritidis expressed a novel porin with a subunit size of 35.5 kDa as shown by SDS-PAGE. This protein was expressed as a major outer membrane protein (MOMP) when grown on nutrient agar, McConkey agar or blood agar, or in Tris-succinate medium; but was only produced in trace amounts when strains ...

journal_title：FEMS microbiology letters

authors： Chart H,Frost JA,Rowe B

更新日期：1993-05-15 00:00:00

abstract：:The choline-containing teichoic and lipoteichoic acids play an important part in cell wall metabolism of Streptococcus pneumoniae. We propose that a choline kinase enzyme has a role in the synthesis of these antigens. The presence of this enzyme was demonstrated in cell free extracts of S. pneumoniae by measuring the ...

journal_title：FEMS microbiology letters

authors： Whiting GC,Gillespie SH

更新日期：1996-05-01 00:00:00

abstract：:A number of hybrids obtained by fusion of protoplasts of complementary auxotrophic strains of Candida albicans were analysed for growth rates, cell volume, DNA content, stability and adherence to human buccal epithelial cells. SDS-PAGE analysis of the cell wall proteins indicated that the hybrid cell walls contained m...

journal_title：FEMS microbiology letters

authors： Law C,Kavanagh K,Whittaker P

更新日期：1994-01-01 00:00:00

abstract：:Agrobacterium tumefaciens strain SCUEC1 is a nicotine-degrading bacterium, which has been recently isolated from the tobacco waste-contaminated field soil. However, the mechanism for nicotine degradation in this strain remains unclear. Here, we analyze the function and biological properties of the agnH gene in the str...

journal_title：FEMS microbiology letters

authors： Xia Z,Yu M,Yao J,Feng Z,Li D,Liu T,Cheng G,He D,Li X

更新日期：2020-02-01 00:00:00

abstract：:Pseudomonas chlororaphis PA23 is a biocontrol agent that, in addition to producing antifungal compounds, synthesizes polyhydroxyalkanoate (PHA) polymers as a carbon and energy sink. Quorum sensing (QS) and the anaerobic regulator (ANR) are required for PA23-mediated fungal suppression; however, the role of these regul...

journal_title：FEMS microbiology letters

authors： Mohanan N,Gislason A,Sharma PK,Ghergab A,Plouffe J,Levin DB,de Kievit T

更新日期：2019-09-01 00:00:00

abstract：:The identification of virulence factors in Rhodococcus equi has been severely hampered by the lack of a method for in vivo random insertion mutagenesis. This study reports the use of transposomes to generate random insertions of a gene conferring kanamycin resistance into the genome of R. equi ATCC 33701. Southern hyb...

journal_title：FEMS microbiology letters

authors： Mangan MW,Meijer WG

更新日期：2001-12-18 00:00:00

abstract：:This communication presents experimental evidence that intact fragile (osmotic sensitive) yeasts can be electrofused and give viable hybrids. The yield increases with one order of magnitude for electrofusion of intact fragile yeasts with protoplasts of non-fragile ones. The yield of viable hybrids, obtained by electro...

journal_title：FEMS microbiology letters

authors： Tsoneva I,Doinov P,Dimitrov DS

更新日期：1989-07-01 00:00:00

abstract：:In Bacillus subtilis, carbon catabolite control is mediated by the regulatory protein CcpA. In addition to loss of catabolite repression, ccpA mutants exhibit a severe growth defect. They are not able to grow with glucose and ammonium as single sources of carbon and nitrogen, respectively. Only ccpA mutant strains car...

journal_title：FEMS microbiology letters

authors： Ludwig H,Stülke J

更新日期：2001-09-11 00:00:00

abstract：:An investigation of mortality of piglets through clinical signs, post-mortem, histopathology and bacteriological analyses revealed the causal organism to be Escherichia coli, mainly O149:K91:K88 which belongs to the enterotoxigenic biotypes. Molecular characterization and epidemiologic analysis elucidated it as shiga-...

journal_title：FEMS microbiology letters

authors： Kanengoni AT,Thomas R,Gelaw AK,Madoroba E

更新日期：2017-02-01 00:00:00

abstract：:Brief exposure of Pseudomonas aeruginosa to a temperature of 10 degrees C or lower caused a significant leakage of the periplasmic beta-lactamase into the medium. The extent of leakage increased as the incubation temperature was lowered to 4 degrees C and reached a maximum at 0 degrees C. Cells grown in the presence o...

journal_title：FEMS microbiology letters

authors： Lei Y,Satake S,Ishii J,Nakae T

更新日期：1991-05-15 00:00:00

abstract：:Helicobacter pylori ATCC43504 responds chemotactically to aspartic acid and serine, but not to arginine, nor to sodium bicarbonate. In contrast, H. pylori ATCC700392 (strain 26695) shows chemotaxis to all four attractants. Open reading frame HP0099 from H. pylori 26695 is predicted to encode one of three methyl-accept...

journal_title：FEMS microbiology letters

authors： Cerda O,Rivas A,Toledo H

更新日期：2003-07-29 00:00:00

abstract：:Although Lentinula edodes is the second most important cultivated mushroom worldwide, most industrially cultivated strains have been identified only through traditional phenotypic analysis. Here, we report for the first time the use of sequence characterized amplified region (SCAR) markers for strain differentiation. ...

journal_title：FEMS microbiology letters

authors： Qin LH,Tan Q,Chen MJ,Pan YJ

更新日期：2006-04-01 00:00:00

abstract：:Saccharomyces cerevisiae was engineered for assembly of minicellulosomes by heterologous expression of a recombinant scaffolding protein from Clostridium cellulovorans and a chimeric endoglucanase E from Clostridium thermocellum. The chimeric endoglucanase E fused with the dockerin domain of endoglucanase B from C. ce...

journal_title：FEMS microbiology letters

authors： Hyeon JE,Yu KO,Suh DJ,Suh YW,Lee SE,Lee J,Han SO

更新日期：2010-09-01 00:00:00

abstract：:Protein interactions among RNA polymerase small subunits from the archaeon Methanococcus jannaschii were investigated using affinity pulldown assays in pairwise and higher-order combinations. In the most extensive study of archaeal RNA polymerase subunit interactions to date, including 37 pairs of proteins, 10 ternary...

journal_title：FEMS microbiology letters

authors： Best AA,Olsen GJ

更新日期：2001-02-05 00:00:00

abstract：:The purpose of this study was to investigate the feasibility of cultivating the biotechnologically important bacterium Streptomyces griseus in single-species and mixed-species biofilms using a tubular biofilm reactor (TBR). Streptomyces griseus biofilm development was found to be cyclical, starting with the initial ad...

journal_title：FEMS microbiology letters

authors： Winn M,Casey E,Habimana O,Murphy CD

更新日期：2014-03-01 00:00:00

abstract：:A plasmid-based food-grade vector system was developed for Lactococcus lactis by exploiting the genes for lactose metabolism. L. lactis MG5267 is a plasmid-free strain containing the entire lactose operon as a chromosomal insertion. The lacF gene was deleted from this strain by a double cross-over homologous recombina...

journal_title：FEMS microbiology letters

authors： MacCormick CA,Griffin HG,Gasson MJ

更新日期：1995-03-15 00:00:00

abstract：:Escherichia coli has been used widely in laboratory and the biotech industry. However, the genetic and metabolic characteristics remain inadequately studied, particularly for those strains with extensive genetic manipulations that might have resulted in unknown mutations. Here, we demonstrate a comparative proteomics ...

journal_title：FEMS microbiology letters

authors： Xia XX,Qian ZG,Lee SY

更新日期：2011-01-01 00:00:00

abstract：:A Caulobacter crescentus rho::Tn5 mutant strain presenting a partially functional transcription termination factor Rho is highly sensitive to hydrogen peroxide in both exponential and stationary phases. The mutant was shown to be permanently under oxidative stress, based on fluorophore oxidation, and also to be sensit...

journal_title：FEMS microbiology letters

authors： Italiani VC,Braz VS,Xiao H,Steinman HM,Marques MV

更新日期：2010-02-01 00:00:00

abstract：:Crithidia oncopelti, Crithidia deanei and Crithidia desouzai are flagellates of the Trypanosomatidae family that present bacterium-like endosymbionts in their cytoplasm. Gelatin-SDS-PAGE analysis was used to characterize cell-associated and extracellular proteinases in these organisms. Our survey indicates that the pr...

journal_title：FEMS microbiology letters

authors： d'Avila-Levy CM,Melo AC,Vermelho AB,Branquinha MH

更新日期：2001-08-07 00:00:00

abstract：:Autophagy, a eukaryotic cellular activity leading to the degradation of cellular components, serves as a defense mechanism against facultative intracellular bacteria as well as a growth niche for the obligate intracellular bacterium Coxiella burnetii. We here demonstrate that the obligate intracellular bacterial patho...

journal_title：FEMS microbiology letters

authors： Pachikara N,Zhang H,Pan Z,Jin S,Fan H

更新日期：2009-03-01 00:00:00

abstract：:Naturally occurring antimicrobial cationic polypeptides play a major role in innate and adaptive immunity. These polypeptides are found to be either linear and unstructured or structured through disulfide bonds. Among the structured antimicrobial polypeptides, defensins comprise a family of cysteine-rich cationic poly...

journal_title：FEMS microbiology letters

authors： Raj PA,Dentino AR

更新日期：2002-01-02 00:00:00

abstract：:The branched-chain amino acid permease Bap2p is a transport system for leucine, isoleucine, and valine in Saccharomyces cerevisiae, and its synthesis is regulated transcriptionally. However, the downregulation mechanisms of Bap2p have not been established. Here we demonstrate that the C-terminal region of Bap2p plays ...

journal_title：FEMS microbiology letters

authors： Omura F,Kodama Y,Ashikari T

更新日期：2001-01-15 00:00:00

abstract：:Sequences from the ribosomal DNA ITS regions have shown that there is limited variation among the species in the Trichoderma viride/atroviride/koningii species complex also known as Hypocrea rufa complex, and that infraspecies variation sometimes is larger than interspecies variation. Strains belonging to T. viride, T...

journal_title：FEMS microbiology letters

authors： Lübeck M,Bulat S,Alekhina I,Lieckfeldt E

更新日期：2004-08-15 00:00:00