Random insertion mutagenesis of the intracellular pathogen Rhodococcus equi using transposomes.

abstract：:The enterobacterium Photorhabdus luminescens produces a number of toxins to kill its insect host. By analyzing the genomic sequence of P. luminescens TT01, we found that amino acid sequences encoded by plu1961 and plu1962 showed high similarity to XaxAB binary toxin of Xenorhabuds nematophila, which has both necrotic ...

journal_title：FEMS microbiology letters

authors： Zhang X,Hu X,Li Y,Ding X,Yang Q,Sun Y,Yu Z,Xia L,Hu S

更新日期：2014-01-01 00:00:00

abstract：:A CO-reactive hemoprotein was purified from the mitochondrial membrane fraction of Tetrahymena pyriformis. It showed absorption peaks at 615 and 455 nm in the reduced form and an alpha peak at 565 nm in the pyridine ferrohemochrome spectrum. Although the spectral properties were apparently similar to those of 'cytochr...

journal_title：FEMS microbiology letters

authors： Inokuchi A,Fukumori Y

更新日期：1993-08-15 00:00:00

abstract：:Streptococcus suis serotype 2 (S. suis 2 or SS2) is the causative agent of several diseases in both pigs and humans. 6-Phosphogluconate dehydrogenase (6PGD) is a cell surface protein in SS2. In this study, the immunogenicity and protective efficacy of recombinant 6PGD (r6PGD) from SS2 was evaluated in piglets. Immuniz...

journal_title：FEMS microbiology letters

authors： Tan C,Liu M,Liu J,Yuan F,Fu S,Liu Y,Jin M,Bei W,Chen H

更新日期：2009-07-01 00:00:00

abstract：:Bacillus sp. strain CS93, which was previously isolated from Pozol, was previously shown to produce iturin A, bacilysin and chlorotetaine. To investigate the biosynthetic mechanism of chlorotetaine production, the bac genes were amplified from genomic DNA of Bacillus sp. CS93 by PCR and sequenced. The genes bacABCDE w...

journal_title：FEMS microbiology letters

authors： Moran S,Robertson K,Paradisi F,Rai DK,Murphy CD

更新日期：2010-03-01 00:00:00

abstract：:The denitrifying bacterium Thauera aromatica strain AR-1 grows anaerobically with protocatechuate (3,4-dihydroxybenzoate (DHB)) as sole energy and carbon source. This bacterium harbors two distinct pathways for degradation of aromatic compounds, the benzoyl-coenzyme A (CoA) pathway for benzoate degradation and the hyd...

journal_title：FEMS microbiology letters

authors： Philipp B,Kemmler D,Hellstern J,Gorny N,Caballero A,Schink B

更新日期：2002-06-18 00:00:00

abstract：:Many antibiotic-producing actinomycetes possess at least one ABC (ATP-binding cassette) transporter which forms part of the antibiotic biosynthetic pathway and in most cases confers resistance to the drug in an heterologous host. Three types of antibiotic ABC transporters have been so far described in producer organis...

journal_title：FEMS microbiology letters

authors： Méndez C,Salas JA

更新日期：1998-01-01 00:00:00

abstract：:Production of the gene transfer agent of Rhodobacter capsulatus, RcGTA, is dependent upon several cellular regulatory systems, including a putative phosphorelay involving the CtrA and CckA proteins. These proteins are also involved in flagellar motility in R. capsulatus. The interactions of proteins in this system are...

journal_title：FEMS microbiology letters

authors： Mercer RG,Quinlan M,Rose AR,Noll S,Beatty JT,Lang AS

更新日期：2012-06-01 00:00:00

abstract：:The constitutive expression vector pHY300-F1gfp was constructed to test the function of a promoter, F1, cloned from the rice epiphyte Bacillus brevis strain DX01. The DX01 cells harboring the plasmid pHY300-F1gfp were shown to produce bright green fluorescence. The results were confirmed by Western blot analysis and f...

journal_title：FEMS microbiology letters

authors： Chen Y,Yan J,Yang M,Wang J,Shen D

更新日期：2003-12-05 00:00:00

abstract：:The polysaccharides formed on hot alkaline extraction of the ascomycetous lichen Teloschistes flavicans were fractionated to give two glucans, which were characterised by methylation analysis and 1D and 2D NMR spectroscopy. One was a branched beta-glucan containing (1-->3) and (1-->6) linkages, a structure which is mo...

journal_title：FEMS microbiology letters

authors： Reis RA,Tischer CA,Gorin PA,Iacomini M

更新日期：2002-04-23 00:00:00

abstract：:Legionella pneumophila is known as a facultative intracellular parasite of free-living soil and freshwater amoebae, of which several species have been shown to support the growth of the pathogenic bacteria. We report for the first time the behaviour of two strains (c2c and Z503) of the amoeba Willaertia magna towards ...

journal_title：FEMS microbiology letters

authors： Dey R,Bodennec J,Mameri MO,Pernin P

更新日期：2009-01-01 00:00:00

abstract：:Aceticlastic methanogens metabolize acetate to methane and carbon dioxide. The central metabolism and the electron transport chains of these organisms have already been investigated. However, no particular attention has been paid to the mechanism by which acetate enters the archaeal cell. In our study we investigated ...

journal_title：FEMS microbiology letters

authors： Welte C,Kröninger L,Deppenmeier U

更新日期：2014-10-01 00:00:00

abstract：:Conditional gene expression systems are useful tools for studying the role of essential or toxic gene products in bacterial systems. There is a paucity of such systems available for use in the mycobacteria. The utility of the Escherichia coli arabinose-inducible system was looked into, since it is tightly controlled i...

journal_title：FEMS microbiology letters

authors： Carroll P,Brown AC,Hartridge AR,Parish T

更新日期：2007-09-01 00:00:00

abstract：:Aspergillus niger represents a promising host for the expression of recombinant proteins, but only a few expression systems are available for this organism. In this study, the inducible catalase promoter (PcatR) from A. niger was characterized. For this, constructs were developed and checked for the expression of the ...

journal_title：FEMS microbiology letters

authors： Sharma R,Katoch M,Govindappa N,Srivastava PS,Sastry KN,Qazi GN

更新日期：2012-02-01 00:00:00

abstract：:Enterobacter cloacae CHE, a clinical strain with overproduced cephalosporinase was found to be highly resistant to the new cephalosporins, cefepime and cefpirome (MICs> or =128 microg ml(-1)). The strain was isolated from a child previously treated with cefepime. The catalytic efficiency of the purified enzyme with th...

journal_title：FEMS microbiology letters

authors： Barnaud G,Labia R,Raskine L,Sanson-Le Pors MJ,Philippon A,Arlet G

更新日期：2001-02-20 00:00:00

abstract：:Chromosomal DNA from 62 strains of 'Campylobacter upsaliensis' from blood and faecal specimens of patients in Australia, Belgium, South Africa and England, and seven dog isolates from England and Sweden were examined. DNA base compositions of 21 strains were 35 +/- 1 mol% G + C. Electrophoresis of HaeIII DNA digests g...

journal_title：FEMS microbiology letters

authors： Owen RJ,Hernandez J

更新日期：1990-10-01 00:00:00

abstract：:Magnetotactic bacteria (MTB) are a group of microorganisms that have the ability to synthesize intracellular magnetic crystals (magnetosomes). They prefer microaerobic or anaerobic aquatic sediments. Thus, there is growing interest in their ecological roles in various habitats. In this study we found co-occurrence of ...

journal_title：FEMS microbiology letters

authors： Pan H,Dong Y,Teng Z,Li J,Zhang W,Xiao T,Wu LF

更新日期：2019-11-01 00:00:00

abstract：:In order to investigate whether the iron acquisition mechanisms of Staphylococcus aureus are induced by iron restriction in vitro, we examined S. aureus ATCC 6538 for production of siderophore and expression of transferrin-binding protein (SA-tbp) in normal or deferrated brain heart infusion broth (BHI). Siderophore p...

journal_title：FEMS microbiology letters

authors： Lim Y,Shin SH,Lee SI,Kim IS,Rhee JH

更新日期：1998-06-01 00:00:00

abstract：:A bacterium strain, which is capable of degrading monocrotophos, was isolated from sludge collected from the bottom of a wastewater treatment system of a chemical factory, and named M-1. On the basis of the results of the cellular morphology, physiological and chemotaxonomic characteristics and phylogenetic similarity...

journal_title：FEMS microbiology letters

authors： Jia KZ,Cui ZL,He J,Guo P,Li SP

更新日期：2006-10-01 00:00:00

abstract：:Three (0140J, C197C and EF20) out of four strains of Streptococcus uberis exhibited high levels of bound plasmin activity following growth in the presence of bovine plasminogen. The remaining strain (C197) bound considerably less plasmin following growth in the same medium. In contrast to the others, this strain was u...

journal_title：FEMS microbiology letters

authors： Leigh JA,Lincoln RA

更新日期：1997-09-01 00:00:00

abstract：:Growth of a Mycobacterium avium complex serotype 20 rough-colony variant in 1.0% Tween 80 resulted in a smooth-colony morphological conversion that was reversible upon removal of Tween and was not associated with the presence of serotype-specific glycopeptidolipid antigens. Electron microscopic examination suggested t...

journal_title：FEMS microbiology letters

authors： Wright EL,Pourshafie M,Barrow WW

更新日期：1992-11-01 00:00:00

abstract：:Astaxanthin possesses higher antioxidant activity than other carotenoids and, for this and other reasons, has great commercial potential for use in the aquaculture, pharmaceutical, and food industries. The basidiomycetous yeast Xanthophyllomyces dendrorhous is one of the best natural producers of astaxanthin, but wild...

journal_title：FEMS microbiology letters

authors： Ukibe K,Katsuragi T,Tani Y,Takagi H

更新日期：2008-09-01 00:00:00

abstract：:Phagemid vectors for display of protein/peptides on the surface of filamentous phage utilize a plasmid genome carrying the phage origin of replication, along with the gene fused to a fragment of gene III. Generation of phage particles displaying the fusion protein also requires superinfection of the host bacterium wit...

journal_title：FEMS microbiology letters

authors： Dueñas M,Borrebaeck CA

更新日期：1995-01-15 00:00:00

abstract：:Haemophilus influenzae has an absolute growth requirement for heme and a heme binding lipoprotein (HbpA) has been implicated in the utilization of this essential nutrient. HbpA was identified by examining clones from an H. influenzae genomic library that caused Escherichia coli harboring the clone to bind heme. Howeve...

journal_title：FEMS microbiology letters

authors： Morton DJ,Madore LL,Smith A,Vanwagoner TM,Seale TW,Whitby PW,Stull TL

更新日期：2005-12-15 00:00:00

abstract：:The structural gene, adhA, for a thermostable primary alcohol dehydrogenase was cloned from Thermoanaerobacter ethanolicus JW200. Constitutive expression from its own promoter was observed in Escherichia coli. The nucleotide sequence of adhA corresponded to an open reading frame of 1197 bp, encoding a polypeptide of 3...

journal_title：FEMS microbiology letters

authors： Holt PJ,Williams RE,Jordan KN,Lowe CR,Bruce NC

更新日期：2000-09-01 00:00:00

abstract：:An obligately anaerobic spirochete designated strain SEBR 4228T (T = type strain) was isolated from an oil field of Congo, Central Africa. The strain grew optimally with a sodium chloride concentration of 5% (sodium chloride concentration) growth range 1.0-10%) at 37 degrees C (growth temperature range 20-40 degrees C...

journal_title：FEMS microbiology letters

authors： Magot M,Fardeau ML,Arnauld O,Lanau C,Ollivier B,Thomas P,Patel BK

更新日期：1997-10-15 00:00:00

abstract：:The expression of nifA-, niH- and nifB-lacZ fusions was examined in different mutants of Azospirillum brasilense. Mutations in nifA, glnA and glnB severely impaired the expression of nifH- and nifB-lacZ fusions. By contrast, a nifA-lacZ fusion was not affected in a nifA or a glnB background and was only partially impa...

journal_title：FEMS microbiology letters

authors： Liang YY,de Zamaroczy M,Arsène F,Paquelin A,Elmerich C

更新日期：1992-12-15 00:00:00

abstract：:The mercury (II) ion is toxic and is usually detoxified in Bacteria by reduction to elemental mercury, which is less toxic. This is catalysed by an NAD(P)H-dependent mercuric reductase (EC 1.16.1.1). Here, we present strong evidence that Methylococcus capsulatus (Bath) - a methanotrophic member of the Gammaproteobacte...

journal_title：FEMS microbiology letters

authors： Boden R,Murrell JC

更新日期：2011-11-01 00:00:00

abstract：:A lysC-lac'Z fusion plasmid was constructed to study the regulatory region of the lysC gene. Analysis by deletion mutations confirmed the existence of an alternative promoter, P2, located upstream of the previously identified promoter, P1. The transcription start site of promoter P2 was located 85 base pairs upstream ...

journal_title：FEMS microbiology letters

authors： Liao HH,Hseu TH

更新日期：1998-11-01 00:00:00

abstract：:The 181 251 bp accessory plasmid pSmeSM11b of Sinorhizobium meliloti strain SM11, belonging to a dominant indigenous S. meliloti subpopulation identified during a long-term field release experiment, was sequenced. This plasmid has 166 coding sequences (CDSs), 42% of which encode proteins with homology to proteins of k...

journal_title：FEMS microbiology letters

authors： Stiens M,Schneiker S,Pühler A,Schlüter A

更新日期：2007-06-01 00:00:00

abstract：:The malate dehydrogenase (MDH; EC 1.1.1.37; L-malate-NAD(+)-oxidoreductase) activities of truffles of the genus Tuber (Tuber melanosporum Vittad., Tuber brumale Vittad., Tuber aestivum Vittad., Tuber magnatum Pico, Tuber rufum Pico) have been characterized with regard to the K(m) and V(max) values in the direct and re...

journal_title：FEMS microbiology letters

authors： Zarivi O,Cesare P,Aimola P,Ragnelli AM,Scirri C,Cimini A,Bonfigli A,Pacioni G,Miranda M

更新日期：2000-04-15 00:00:00