Abstract:
:Saccharomyces cerevisiae was engineered for assembly of minicellulosomes by heterologous expression of a recombinant scaffolding protein from Clostridium cellulovorans and a chimeric endoglucanase E from Clostridium thermocellum. The chimeric endoglucanase E fused with the dockerin domain of endoglucanase B from C. cellulovorans was assembled with the recombinant scaffolding protein. The resulting strain was able to ferment amorphous cellulose [carboxymethyl-cellulose (CMC)] into ethanol with the aid of beta-glucosidase 1 produced from Saccharomycopsis fibuligera. The minicellulosomes assembled in vivo retained the synergistic effect for cellulose hydrolysis. The minicellulosomes containing the cellulose-binding domain were purified by crystalline cellulose affinity in a single step. In the fermentation test at 10 g L(-1) initial CMC, approximately 3.45 g L(-1) ethanol was produced after 16 h. The yield (in grams of ethanol produced per substrate) was 0.34 g g(-1) from CMC. This result indicates that a one-step processing of cellulosic biomass in a consolidated bioprocessing configuration is technically feasible by recombinant yeast cells expressing functional minicellulosomes.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Hyeon JE,Yu KO,Suh DJ,Suh YW,Lee SE,Lee J,Han SOdoi
10.1111/j.1574-6968.2010.02035.xsubject
Has Abstractpub_date
2010-09-01 00:00:00pages
39-47issue
1eissn
0378-1097issn
1574-6968pii
FML2035journal_volume
310pub_type
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