Comparison of bacterial flora and enzymatic activity in faeces of infants and calves.

abstract：:Two hybridoma cell lines producing monoclonal antibodies (MAbs) against a hemagglutinin/protease (HA/P) from Vibrio cholerae non-01 were produced and characterized. The two MAbs contained the kappa light chain and were IgG1 type. They similarly neutralized HA/P protease activity derived from both V. cholerae non-01 an...

journal_title：FEMS microbiology letters

authors： Honda T,Hata-Naka A,Lertpocasombat K,Miwatani T

更新日期：1991-03-01 00:00:00

abstract：:The first prokaryotic urea carboxylase has previously been purified and characterized from Oleomonas sagaranensis. As the results indicated the presence of an ATP-dependent urea degradation pathway in Bacteria, the characterization of the second component of this pathway, allophanate hydrolase, was carried out. The ge...

journal_title：FEMS microbiology letters

authors： Kanamori T,Kanou N,Kusakabe S,Atomi H,Imanaka T

更新日期：2005-04-01 00:00:00

abstract：:It was shown that virulent Mycobacterium tuberculosis H37Rv induces necrosis of infected RAW264 cells at 24 h post infection while avirulent H37Ra and an attenuated H37Rv mutant that is deficient for RD1 region (H37RvDeltaRD1) cause less necrosis of the infected cells. While H37Rv caused damage of the mitochondrial in...

journal_title：FEMS microbiology letters

authors： Kaku T,Kawamura I,Uchiyama R,Kurenuma T,Mitsuyama M

更新日期：2007-09-01 00:00:00

abstract：:Staphylococcus aureus produces a number of virulence factors. The major virulence factors exhibited by S aureus include various antigens, enzymes, cytotoxins and exotoxins (e.g. hemolysins, enterotoxins and toxic shock syndrome toxin). In this report, we show the influence of punicalagin on the secretion of exoprotein...

journal_title：FEMS microbiology letters

authors： Mun SH,Kong R,Seo YS,Zhou T,Kang OH,Shin DW,Kwon DY

更新日期：2016-11-01 00:00:00

abstract：:Fluorescence in situ hybridisation (FISH) has become one of the major techniques in environmental microbiology. The original version of this technique often suffered from limited sensitivity due to low target copy number or target inaccessibility. In recent years there have been several developments to amend this prob...

journal_title：FEMS microbiology letters

authors： Zwirglmaier K

更新日期：2005-05-15 00:00:00

abstract：:Salmonella enterica serovar Enteritidis (S. Enteritidis) possesses plasmids of different sizes and roles. Besides the serovar-specific virulence plasmid present in most field strains, S. Enteritidis can harbour plasmids of low molecular mass whose biological role is poorly understood. We therefore sequenced plasmid pC...

journal_title：FEMS microbiology letters

authors： Gregorova D,Pravcova M,Karpiskova R,Rychlik I

更新日期：2002-09-10 00:00:00

abstract：:In a large-scale gene disruption screen of Magnaporthe oryzae, a gene MoST1 encoding a protein belonging to the hexose transporter family was identified as a gene required for conidiation and culture pigmentation. The gene MoST1 located on chromosome V of the M. oryzae genome was predicted to be 1892 bp in length with...

journal_title：FEMS microbiology letters

authors： Saitoh H,Hirabuchi A,Fujisawa S,Mitsuoka C,Terauchi R,Takano Y

更新日期：2014-03-01 00:00:00

abstract：:Mild alkaline solutions (beta-elimination), after removing the non-covalently bonded wall materials by hot SDS, released 13% and 26% of remaining wall proteins from mycelial and yeast cells of Candida albicans, respectively. When the beta-elimination was carried out after digestion of the walls with chitinase, four-fo...

journal_title：FEMS microbiology letters

authors： Mormeneo S,Marcilla A,Iranzo M,Sentandreu R

更新日期：1994-10-15 00:00:00

abstract：:It is known that certain yeast strains, so called 'killers', can produce and excrete proteinaceous toxins that can induce death of other sensitive strains. We obtained a stable fungicidal factor (SKF) through concentration and stabilization of the excretion product of certain killer strains of Saccharomyces cerevisiae...

journal_title：FEMS microbiology letters

authors： Bracesco N,Salvo VA,Carrau FM,Nunes E

更新日期：2006-03-01 00:00:00

abstract：:A chromosomal DNA fragment from Streptomyces antibioticus ATCC11891 was isolated by its ability to stimulate actinorhodin and undecylprodigiosin biosynthesis in Streptomyces lividans TK21. This fragment includes two open reading frames, whose deduced translated products resemble enzymes involved in sulfur metabolism (...

journal_title：FEMS microbiology letters

authors： Scheu AK,Martínez E,Soliveri J,Malpartida F

更新日期：1997-02-01 00:00:00

abstract：:Stress-activated MAPK pathways are systems used to regulate the stress adaptation of most fungi. It has been shown that in Talaromyces marneffei (Penicillium marneffei), a pathogenic dimorphic fungus, the sakA gene is involved, not only in tolerance against oxidative and heat stresses, but also in playing a role in as...

journal_title：FEMS microbiology letters

authors： Nimmanee P,Tam EWT,Woo PCY,Vanittanakom P,Vanittanakom N

更新日期：2017-04-01 00:00:00

abstract：:The ecology of aerobic microorganisms is never explored in marine oxygen minimum zone (OMZ) sediments. Here we reveal aerobic bacterial communities along ∼3 m sediment-horizons of the eastern Arabian Sea OMZ. Sulfide-containing sediment-cores retrieved from 530 mbsl (meters beneath the sea-level) and 580 mbsl were exp...

journal_title：FEMS microbiology letters

authors： Bhattacharya S,Roy C,Mandal S,Sarkar J,Rameez MJ,Mondal N,Mapder T,Chatterjee S,Pyne P,Alam M,Haldar PK,Roy R,Fernandes S,Peketi A,Chakraborty R,Mazumdar A,Ghosh W

更新日期：2020-10-16 00:00:00

abstract：:We have isolated the abc1 gene from the fission yeast Schizosaccharomyces pombe. Sequence analysis suggests that the Abc1 protein is a member of the ABC superfamily of transporters and is composed of two structurally homologous halves, each consisting of a hydrophobic region of six transmembrane domains and a hydrophi...

journal_title：FEMS microbiology letters

authors： Christensen PU,Davis K,Nielsen O,Davey J

更新日期：1997-02-01 00:00:00

abstract：:We developed a multiplex PCR to detect the presence of methicillin- (mecA), cadmium/zinc-(czrC) and antiseptic-resistant (qacA/B) staphylococci and to identify Panton-Valentine leukocidin (PVL)-positive and -negative Staphylococcus aureus and coagulase-negative staphylococci (CoNS) from infected and healthy eyes. The ...

journal_title：FEMS microbiology letters

authors： Panda S,Kar S,Choudhury R,Sharma S,Singh DV

更新日期：2014-03-01 00:00:00

abstract：:The filamentous fungus Cunninghamella elegans has the ability to metabolize xenobiotics, including polycyclic aromatic hydrocarbons and pharmaceutical drugs, by both phase I and II biotransformations. Cytosolic and microsomal fractions were assayed for activities of cytochrome P450 monooxygenase, aryl sulfotransferase...

journal_title：FEMS microbiology letters

authors： Zhang D,Yang Y,Leakey JE,Cerniglia CE

更新日期：1996-05-01 00:00:00

abstract：:In order to characterize the metabolites produced in vivo by biphenyl-2,3-dioxygenase and biphenyl-2,3-dihydrodiol-2,3-dehydrogenase, the first two enzymes of the (polychloro)biphenyl catabolic pathway encoded by the bph locus of Pseudomonas sp. LB400, recombinant E. coli strains expressing the respective genes were c...

journal_title：FEMS microbiology letters

authors： Seeger M,Timmis KN,Hofer B

更新日期：1995-11-15 00:00:00

abstract：:Mycoplasma mycoides subsp. mycoides (Mmm) strain Afadé had previously been shown to undergo spontaneous phase variations between an opaque capsulated variant and a translucent (TR) variant devoid of a capsule but able to secrete cell-free exopolysaccharides. This phase variation is associated with an ON/OFF genetic sw...

journal_title：FEMS microbiology letters

authors： Gaurivaud P,Lakhdar L,Le Grand D,Poumarat F,Tardy F

更新日期：2014-10-01 00:00:00

abstract：:Trichomonas vaginalis is a protozoan that parasitizes the human urogenital tract causing trichomoniasis, the most common non-viral sexually transmitted disease. The parasite has unique genomic characteristics such as a large genome size and expanded gene families. Ectonucleoside triphosphate diphosphohydrolase (E-NTPD...

journal_title：FEMS microbiology letters

authors： Frasson AP,Dos Santos O,Meirelles LC,Macedo AJ,Tasca T

更新日期：2016-01-01 00:00:00

abstract：:To exert a beneficial effect on the host, adequate doses of probiotics must be administered and maintaining their viability until consumption is thus essential. Dehydrated probiotics exhibit enhanced long-term viability and can be incorporated into low-moisture food matrices, which also possess high stability at refri...

journal_title：FEMS microbiology letters

authors： Marcial-Coba MS,Knøchel S,Nielsen DS

更新日期：2019-01-01 00:00:00

abstract：:To improve expression efficiency of the leuB gene of an extreme thermophile. Thermus thermophilus, in Escherichia coli, the gene was placed under a potent promoter, tac. However, the expression was hardly improved, despite increased transcription. The expression under tac promoter was significantly improved by introdu...

journal_title：FEMS microbiology letters

authors： Ishida M,Oshima T

更新日期：1996-01-01 00:00:00

abstract：:Escherichia coli mutant MM52 (secA(ts)) was transformed with a cosmid library from Staphylococcus carnosus, and a recombinant cosmid (pBO23) allowing growth at the non-permissive temperature (42 degrees C) was isolated. pBO23 also restored the growth defects of E. coli mutants IQ85 (secY(ts)) and IT41 (lep(ts)). Nucle...

journal_title：FEMS microbiology letters

authors： Overhoff-Freundlieb B,Freudl R

更新日期：1991-11-15 00:00:00

abstract：:Type II restriction endonucleases were purified by heparin-sepharose followed by ion chromatography from Treponema strains. The results indicate that in addition to frequently cutting GATC-specific restriction enzymes, the tested strains also possess rarely cutting endonucleases. The purified restriction endonucleases...

journal_title：FEMS microbiology letters

authors： Piknova M,Javorsky P,Pristas P

更新日期：2005-10-01 00:00:00

abstract：:We have investigated the iron-dependent regulation of the psbA gene, encoding the enzyme L-ornithine N(5)-oxygenase in the rhizobacterium Pseudomonas B10. We have cloned and characterized a Pseudomonas B10 gene, designated psbS, required for psbA expression. PsbS is endowed with structural and functional features of e...

journal_title：FEMS microbiology letters

authors： Leoni L,Ambrosi C,Petrucca A,Visca P

更新日期：2002-03-05 00:00:00

abstract：:We found two Shiga toxin producing Escherichia coli O157:H7 strains isolated from humans carrying the stx(1) gene with an IS1203-like element (designated as IS1203v(1)). The IS1203v(1) was inserted into the coding region of the A subunit 7 bp upstream from the TGA termination codon, resulting in a loss of two amino ac...

journal_title：FEMS microbiology letters

authors： Suzuki M,Kondo F,Ito Y,Matsumoto M,Hata M,Oka H,Takahashi M,Sakae K

更新日期：2004-05-01 00:00:00

abstract：:Crithidia oncopelti, Crithidia deanei and Crithidia desouzai are flagellates of the Trypanosomatidae family that present bacterium-like endosymbionts in their cytoplasm. Gelatin-SDS-PAGE analysis was used to characterize cell-associated and extracellular proteinases in these organisms. Our survey indicates that the pr...

journal_title：FEMS microbiology letters

authors： d'Avila-Levy CM,Melo AC,Vermelho AB,Branquinha MH

更新日期：2001-08-07 00:00:00

abstract：:The discovery that all hyperthermophiles that have been evaluated have the capacity to reduce Fe(III) has raised the question of whether mechanisms for dissimilatory Fe(III) reduction have been conserved throughout microbial evolution. Many studies have suggested that c-type cytochromes are integral components in elec...

journal_title：FEMS microbiology letters

authors： Childers SE,Lovley DR

更新日期：2001-02-20 00:00:00

abstract：:The extensively discussed idea of oxidative stress development under antibiotic treatment was confirmed using an antioxidant gene expression (soxRS-, oxyR-regulon) approach, including microaerobic cultivation conditions. The killing action of antibiotics and their ability to cause peroxide oxidative stress in Escheric...

journal_title：FEMS microbiology letters

authors： Akhova AV,Tkachenko AG

更新日期：2014-04-01 00:00:00

abstract：:We screened for a gene that inhibits streptomycin production in Streptomyces griseus when it is introduced on a high-copy-number plasmid pIJ702, and obtained a plasmid pKM545. The introduction of pKM545 abolished streptomycin production on all media tested including YMP-sugar and Nutrient broth. S1 protection analysis...

journal_title：FEMS microbiology letters

authors： Takano H,Toriumi N,Hirata M,Amano T,Ohya T,Shimada R,Kusada H,Amano S,Matsuda K,Beppu T,Ueda K

更新日期：2016-07-01 00:00:00

abstract：:Helicobacter pylori, a type 1 carcinogen, accounts for numerous gastric cancer-related deaths worldwide. Repurposing existing drugs or developing new ones for a combinatorial approach against increasing antimicrobial resistance is the need of the hour. This study highlights the efficacy of acriflavine hydrochloride (A...

journal_title：FEMS microbiology letters

authors： Tehlan A,Karmakar BC,Paul S,Kumar R,Kaur I,Ghosh A,Mukhopadhyay AK,Dhar SK

更新日期：2020-11-23 00:00:00

abstract：:The number and incidence of Collinsella aerofaciens in the human intestine are the highest among Gram-positive non-spore-forming bacilli. Identification of this species is very difficult and requires considerable time. A PCR-based identification system using C. aerofaciens-specific primers is described. Using this PCR...

journal_title：FEMS microbiology letters

authors： Kageyama A,Sakamoto M,Benno Y

更新日期：2000-02-01 00:00:00