Antibacterial action of acriflavine hydrochloride for eradication of the gastric pathogen Helicobacter pylori.

Abstract:

:Helicobacter pylori, a type 1 carcinogen, accounts for numerous gastric cancer-related deaths worldwide. Repurposing existing drugs or developing new ones for a combinatorial approach against increasing antimicrobial resistance is the need of the hour. This study highlights the efficacy of acriflavine hydrochloride (ACF-HCl) in inhibiting the growth of H. pylori reference strain and antibiotic-resistant clinical isolates at low concentrations. ACF-HCl inhibits H. pylori growth at MIC value 10 times less than that in Escherichia coli, another Gram-negative bacteria. Furthermore, ACF-HCl demonstrates synergistic effect with clarithromycin, a commonly used antibiotic against H. pylori. ACF-HCl treatment also eradicates H. pylori infection in the mice model efficiently. Our in vitro data indicate that bacterial membrane is the prime target. The novel action of ACF-HCl against antibiotic-resistant clinical isolates, synergistic effect with the conventional antibiotic clarithromycin and eradication of H. pylori from infected mice highlight the potential of ACF-HCl as a promising therapeutic agent against H. pylori by itself as well as for combinatorial therapy.

journal_name

FEMS Microbiol Lett

authors

Tehlan A,Karmakar BC,Paul S,Kumar R,Kaur I,Ghosh A,Mukhopadhyay AK,Dhar SK

doi

10.1093/femsle/fnaa178

subject

Has Abstract

pub_date

2020-11-23 00:00:00

issue

21

eissn

0378-1097

issn

1574-6968

pii

5942657

journal_volume

367

pub_type

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