Identification of a Shiga-toxin type I variant containing an IS1203-like element, from Shiga-toxin producing Escherichia coli O157:H7.

Abstract:

:We found two Shiga toxin producing Escherichia coli O157:H7 strains isolated from humans carrying the stx(1) gene with an IS1203-like element (designated as IS1203v(1)). The IS1203v(1) was inserted into the coding region of the A subunit 7 bp upstream from the TGA termination codon, resulting in a loss of two amino acid residues (Ser-Ser) from its C terminus. Toxicity of the Stx1 was confirmed by Vero cell assay. IS1203v(1) hardly affected the stx(1) gene in either its expression or the toxicity of its product.

journal_name

FEMS Microbiol Lett

authors

Suzuki M,Kondo F,Ito Y,Matsumoto M,Hata M,Oka H,Takahashi M,Sakae K

doi

10.1016/j.femsle.2004.03.008

keywords:

subject

Has Abstract

pub_date

2004-05-01 00:00:00

pages

63-7

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378109704001922

journal_volume

234

pub_type

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