Fluorescence in situ hybridisation (FISH)--the next generation.

Abstract:

:Fluorescence in situ hybridisation (FISH) has become one of the major techniques in environmental microbiology. The original version of this technique often suffered from limited sensitivity due to low target copy number or target inaccessibility. In recent years there have been several developments to amend this problem by increasing signal intensity. This review summarises various approaches for signal amplification, focussing especially on two widely recognised varieties, tyramide signal amplification and multiply labelled polynucleotide probes. Furthermore, new applications for FISH are discussed, which arise from the increased sensitivity of the method.

journal_name

FEMS Microbiol Lett

authors

Zwirglmaier K

doi

10.1016/j.femsle.2005.04.015

keywords:

subject

Has Abstract

pub_date

2005-05-15 00:00:00

pages

151-8

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(05)00237-5

journal_volume

246

pub_type

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