ATP/ADP alteration as a sign of the oxidative stress development in Escherichia coli cells under antibiotic treatment.

Abstract:

:The extensively discussed idea of oxidative stress development under antibiotic treatment was confirmed using an antioxidant gene expression (soxRS-, oxyR-regulon) approach, including microaerobic cultivation conditions. The killing action of antibiotics and their ability to cause peroxide oxidative stress in Escherichia coli cells was comparable to a similar hydrogen peroxide capacity; therefore, the involvement of intracellular hydrogen peroxide production in the killing action of antibiotics seems plausible under conditions studied. The temporary increase of ATP/ADP (which returned to untreated levels in 10 min) and the intensification of respiration preceded the development of oxidative stress. The sharp rise in ATP/ADP was due to the accumulation of ATP with a slight increase in the ADP content. We proposed that ATP accumulation was not a result of increased respiration but was due to the inhibition of energy-consuming processes. The association of reactive oxygen species formation under antibiotic treatment with the inhibition of direct electron flow pathway along the respiratory chain, and a possible role of a sharp rise in ATP/ADP in this process is hypothesized.

journal_name

FEMS Microbiol Lett

authors

Akhova AV,Tkachenko AG

doi

10.1111/1574-6968.12405

subject

Has Abstract

pub_date

2014-04-01 00:00:00

pages

69-76

issue

1

eissn

0378-1097

issn

1574-6968

journal_volume

353

pub_type

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