Abstract:
:A 1.5-kb XbaI-SacII fragment containing the upstream region of the Trichoderma reesei cellobiohydrolase I gene (cbh1) has been sequenced. The 1.5-kb fragment contains eight 6-bp sites having an identical or similar sequence to the consensus sequence for binding a catabolite repressor, Aspergillus nidulans CreA. Results of binding assays with the maltose-binding protein::Cre1(10-131) fusion protein (Cre1 is a catabolite repressor of T. reesei) and the cbh1 upstream region revealed that a 504-bp XbaI-NspV fragment (nucleotide position -1496 to -993) bearing three 6-bp sites, A1, A2, and A3, and a 356-bp NspV-MunI fragment (nucleotide position -994 to -639) bearing three 6-bp sites, B1, B2, and B3, were shifted in the electrophoretic mobility shift assay. DNase I footprinting experiments showed that the 6-bp sites A2, B1, B2, and B3 were protected from DNase I digestion.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Takashima S,Iikura H,Nakamura A,Masaki H,Uozumi Tdoi
10.1111/j.1574-6968.1996.tb08601.xsubject
Has Abstractpub_date
1996-12-15 00:00:00pages
361-6issue
3eissn
0378-1097issn
1574-6968pii
S0378109796004326journal_volume
145pub_type
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