Constitutive transcription of the gene for metallothionein in a cadmium-resistant yeast.

Abstract:

:A cadmium-resistant strain of Saccharomyces cerevisiae produces a cadmium metallothionein encoded by the CUP1 gene as does a copper-resistant strain. The mechanism of expression of the gene is inducible by copper ions in the copper-resistant strain. However, assays of CUP1-specific mRNA revealed that the transcription of the CUP1 gene in the cadmium-resistant strain is constitutive and the rate of transcription is further increased by exposure to cadmium or copper ions. This result was confirmed by the appearance of constitutive-expression segregants from diploid crosses between the cadmium-resistant strain and a strain with a reporter gene having the promoter of CUP1.

journal_name

FEMS Microbiol Lett

authors

Tohoyama H,Inagawa A,Koike H,Inouhe M,Joho M,Murayama T

doi

10.1016/0378-1097(92)90740-f

keywords:

subject

Has Abstract

pub_date

1992-08-01 00:00:00

pages

81-5

issue

1

eissn

0378-1097

issn

1574-6968

journal_volume

74

pub_type

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