Synthesis of poly(3-hydroxyalkanoates) in Escherichia coli expressing the PHA synthase gene phaC2 from Pseudomonas aeruginosa: comparison of PhaC1 and PhaC2.

abstract：:The Saccharomyces sensu stricto genus contains many species that are industrially important for fermentation of wines, beers and ales. The molecular characterization of the genomes of yeasts involved in these processes reveals that the majority arose from interspecific hybridization between two and sometimes three yea...

journal_title：FEMS microbiology letters

authors： Querol A,Bond U

更新日期：2009-04-01 00:00:00

abstract：:Twelve strains of Candida albicans were grown in defined medium which had been deferrated by ion-exchange chromatography and then supplemented with FeCl3 to give iron concentrations ranging from 0.026 microM (growth-limiting) to 0.8 microM (excess). All of the strains secreted hydroxamate-type siderophores; phenolate ...

journal_title：FEMS microbiology letters

authors： Sweet SP,Douglas LJ

更新日期：1991-05-01 00:00:00

abstract：:Characterization of genomic variation among different microbial species, or different strains of the same species, is a field of significant interest with a wide range of potential applications. We have investigated the genomic variation in mycorrhizal fungal genomes through genomic suppressive subtractive hybridizati...

journal_title：FEMS microbiology letters

authors： Murat C,Zampieri E,Vallino M,Daghino S,Perotto S,Bonfante P

更新日期：2011-05-01 00:00:00

abstract：:In Bacillus subtilis, carbon catabolite control is mediated by the regulatory protein CcpA. In addition to loss of catabolite repression, ccpA mutants exhibit a severe growth defect. They are not able to grow with glucose and ammonium as single sources of carbon and nitrogen, respectively. Only ccpA mutant strains car...

journal_title：FEMS microbiology letters

authors： Ludwig H,Stülke J

更新日期：2001-09-11 00:00:00

abstract：:Two yeasts from the effluent of a uranium mineral heap were identified as Candida sorbophila and Rhodotorula mucilaginosa. They are well adapted to such an environment as shown by their tolerance to metals and acid pH. However, different mechanisms could be involved in the adaptation: C. sorbophila could be considered...

journal_title：FEMS microbiology letters

authors： de Silóniz MI,Payo EM,Callejo MA,Marquina D,Peinado JM

更新日期：2002-05-07 00:00:00

abstract：:Rhizobium sp. SIN-1, isolated in India from root nodules on the tropical legume Sesbania aculeata, also induces nitrogen-fixing nodules on roots of S. macrocarpa, S. speciosa, S. procumbens, S. punicea, S. rostrata, and Vigna unguiculata. Unlike Azorhizobium caulinodans, SIN-1 does not induce stem nodules on S. rostra...

journal_title：FEMS microbiology letters

authors： Rana D,Krishnan HB

更新日期：1995-12-01 00:00:00

abstract：:Chronological analysis of 125 Vibrio cholerae O139 strains isolated during 1993-2005 in Kolkata revealed the prevalence of two new genotypes of cholera toxin (CT) and novel combinations of ctxB and rstR alleles resulting in variant CTX prophages. One of the new genotypes of ctxB, which first appeared in 1996 with the ...

journal_title：FEMS microbiology letters

authors： Raychoudhuri A,Mukherjee P,Ramamurthy T,Nandy RK,Takeda Y,Nair GB,Mukhopadhyay AK

更新日期：2010-02-01 00:00:00

abstract：:The pseudotrisaccharide allosamidin, a potent inhibitor of chitinases, retarded the fragmentation of hyphae but did not affect the fungal growth and cephalosporin-C production in Acremonium chrysogenum. In vitro inhibition of A. chrysogenum cell-bound chitinase(s) by allosamidin revealed that about 47% of the soluble ...

journal_title：FEMS microbiology letters

authors： Sándor E,Pusztahelyi T,Karaffa L,Karányi Z,Pócsi I,Biró S,Szentirmai A,Pócsi I

更新日期：1998-07-15 00:00:00

abstract：:The ability to identify pathogenic organisms rapidly provides significant benefits to clinicians; in particular, with respect to best prescription practices and tracking of recurrent infections. Conventional bioassays require 3-5 days before identification of an organism can be made, thus compromising the effectivenes...

journal_title：FEMS microbiology letters

authors： Jarvis RM,Goodacre R

更新日期：2004-03-19 00:00:00

abstract：:pH is a key environmental factor affecting the physiology, ecology and pathogenicity of the oral biofilms colonizing the hard tissues of the human mouth. Much attention has been focused on the production of organic acids through the metabolism of carbohydrates by pathogenic oral bacteria. Now, evidence is emerging tha...

journal_title：FEMS microbiology letters

authors： Burne RA,Marquis RE

更新日期：2000-12-01 00:00:00

abstract：:The aim of this study was the production of the homopolyester poly(4-hydroxybutyric acid) (poly(4HB)) with recombinant strains of Escherichia coli. Wild-type strains and other widely used non-recombinant strains of E. coli are not able to produce polyhydroxyalkanoic acids (PHA) as storage compounds and cannot utilize ...

journal_title：FEMS microbiology letters

authors： Hein S,Söhling B,Gottschalk G,Steinbüchel A

更新日期：1997-08-15 00:00:00

abstract：:TraJ is an activator of the transfer (tra) operon in the F plasmid that counteracts H-NS silencing at the main transfer promoter (P(Y)). TraJ contains 226 aa (26 670 kDa), not 229 aa as reported previously, and forms homodimers. TraJ binds DNA containing P(Y)in vivo as demonstrated using a chromatin-immunoprecipitatio...

journal_title：FEMS microbiology letters

authors： Rodriguez-Maillard JM,Arutyunov D,Frost LS

更新日期：2010-09-01 00:00:00

abstract：:Listeria monocytogenes is a Gram positive pathogen that is ubiquitous in the environment. It is a facultative anaerobic rod that causes listeriosis, a disease with potentially lethal consequences for susceptible individuals. During infection, the pathogen is capable of sequestering metal ions to act as vital biocataly...

journal_title：FEMS microbiology letters

authors： Dowd GC,Casey PG,Begley M,Hill C,Gahan CG

更新日期：2012-02-01 00:00:00

abstract：:In previous work, only one culture (strain TA12) from a pristine site was reported to utilize the xenobiotic compound p-toluenesulfonate (TSA) as a sole source of carbon and energy for aerobic growth. 'Strain TA12' has now been recognized as a community of three bacteria: Achromobacter xylosoxidans TA12-A, Ensifer adh...

journal_title：FEMS microbiology letters

authors： Tralau T,Yang EC,Tralau C,Cook AM,Küpper FC

更新日期：2011-03-01 00:00:00

abstract：:The nucleotide sequence of the gene specifying the ethidium efflux system of Escherichia coli has been determined. The translated open reading frame has identified a membrane-bound polypeptide of 110 amino acids (11,960 Da) which shares 42% identity with a staphylococcal protein specifying resistance to ethidium. ...

journal_title：FEMS microbiology letters

authors： Purewal AS

更新日期：1991-08-01 00:00:00

abstract：:The 2.5-kb erythromycin resistance (EmR) plasmid pPV142 of Staphylococcus simulans 13044 was isolated and characterized. Sequence analysis identified ORF1 and ORF2 encoding a 158-residue replication protein (Rep142) and a 244-residue erythromycin resistance protein (Erm, rRNA adenine N-6-methyltransferase), respective...

journal_title：FEMS microbiology letters

authors： Somkuti GA,Solaiman DK,Steinberg DH

更新日期：1998-08-15 00:00:00

abstract：:A cloning cassette, carrying a modified amino-glycoside resistance gene (neo) from transposon Tn5 was constructed. Three restriction sites internal to the neo gene were eliminated by in vitro mutagenesis, allowing their use in designing new cloning vectors. The original, suboptimal transcription promoter was replaced ...

journal_title：FEMS microbiology letters

authors： Denis F,Brzezinski R

更新日期：1991-07-01 00:00:00

abstract：:A cold shock domain (CSD)-containing protein, CspD, of molecular mass ~7.28 kDa in a psychrotolerant Antarctic Janthinobacterium sp. Ant5-2 (ATCC BAA-2154) exhibited constitutive expression at 37, 22, 15, 4 and -1°C. The cspD gene encoding the CspD protein of Ant5-2 was cloned, sequenced and analyzed. The deduced prot...

journal_title：FEMS microbiology letters

authors： Mojib N,Andersen DT,Bej AK

更新日期：2011-06-01 00:00:00

abstract：:Neisseria meningitidis is able to chelate iron from human transferrin (HTF), the main sequestrator of extracellular iron in vivo. Previous workers have reported that a ca. 70 kilodalton (kDa) iron regulated outer membrane protein (FeRP-70) is a highly specific receptor for HTF. We have examined the interaction between...

journal_title：FEMS microbiology letters

authors： Ala'Aldeen DA,Davies HA,Wall RA,Borriello SP

更新日期：1990-05-01 00:00:00

abstract：:The copZ gene of Bacillus subtilis encodes a copper chaperon CopZ that donates copper to the copper transporter CopA. Both genes copZ and copA are clustered to an operon and its promoter is regulated by Cu ions and CueR, a Mer-like transcriptional activator. Here we show that cadmium ions activate copZA expression as ...

journal_title：FEMS microbiology letters

authors： Solovieva IM,Entian KD

更新日期：2004-07-01 00:00:00

abstract：:We have previously reported that mycolyl glycolipids from Nocardia rubra such as glucose or trehalose mycolates induced granuloma formation in mice. The structure of the carbohydrate moiety of the mycolyl glycolipids influenced the granuloma forming activity profoundly. Here, we have examined the macrophage-chemotacti...

journal_title：FEMS microbiology letters

authors： Matsunaga I,Oka S,Inoue T,Yano I

更新日期：1990-01-15 00:00:00

abstract：:Biological halogenation of aromatic compounds implies the generation of reducing equivalents in the form of e.g. NADH. Thermodynamic calculations show that coupling the halogenation step to a step in which the reducing equivalents are oxidized with a potent oxidant such as O2 or N2O makes the halogenation reaction the...

journal_title：FEMS microbiology letters

authors： Dolfing J

更新日期：1998-10-15 00:00:00

abstract：:It has been widely reported that pH mediates cadmium toxicity to bacteria. We used a tripartite approach to investigate mechanisms by which pH affects cadmium toxicity that included analyses of: (1) growth kinetics, (2) global gene expression, and (3) cadmium speciation. Cadmium extended the lag phase at pH 7, but not...

journal_title：FEMS microbiology letters

authors： Worden CR,Kovac WK,Dorn LA,Sandrin TR

更新日期：2009-04-01 00:00:00

abstract：:A two-step enzymatic synthesis process of 4-hydroxyisoleucine is suggested. In the first step, the aldol condensation of acetaldehyde and alpha-ketobutyrate catalyzed by specific aldolase results in the formation of 4-hydroxy-3-methyl-2-keto-pentanoate (HMKP). In the second step, amination of HMKP by the branched-chai...

journal_title：FEMS microbiology letters

authors： Smirnov SV,Samsonova NN,Novikova AE,Matrosov NG,Rushkevich NY,Kodera T,Ogawa J,Yamanaka H,Shimizu S

更新日期：2007-08-01 00:00:00

abstract：:Pseudomonas sp. TLC6-6.5-4 is a multiple metal resistant plant growth-promoting bacteria isolated from copper-contaminated lake sediments. In this study, a comprehensive analysis of genes involved in copper resistance was performed by generating a library of transposon (Tn5) mutants. Two copper-sensitive mutants with ...

journal_title：FEMS microbiology letters

authors： Li K,Pidatala RR,Ramakrishna W

更新日期：2012-10-01 00:00:00

abstract：:In the purple sulphur bacterium Allochromatium vinosum, the prosthetic group of dissimilatory sulphite reductase (DsrAB) was identified as siroamide, an amidated form of the classical sirohaem. The genes dsrAB are the first two of a large cluster of genes necessary for the oxidation of sulphur globules stored intracel...

journal_title：FEMS microbiology letters

authors： Lübbe YJ,Youn HS,Timkovich R,Dahl C

更新日期：2006-08-01 00:00:00

abstract：:Macrolide is inactivated with ATP plus crude extract of Escherichia coli producing macrolide 2'-phosphotransferase (MPH(2')), but not by living cells. Therefore, a convenient method for detection of MPH(2') using intact cells is needed. In this report, we determine that the modified lysozyme-DNase-RNase (LDR) method (...

journal_title：FEMS microbiology letters

authors： Taniguchi K,Nakamura A,Tsurubuchi K,O'Hara K,Sawai T

更新日期：1998-10-15 00:00:00

abstract：:Sphingobacterium sp. RB, a novel bacterial strain isolated from a soil sample, was able to utilize para-nitrophenol (PNP) as sole source of carbon and energy at high concentrations (1.0-5.0 mM). The culture completely degraded 3.0 mM PNP within 36 h with proportionate increase in biomass. With 5.0 mM PNP (700 ppm), 70...

journal_title：FEMS microbiology letters

authors： Samson R,Bodade R,Zinjarde S,Kutty R

更新日期：2019-07-01 00:00:00

abstract：:Cells of the pel1 mutant of Saccharomyces cerevisiae were found to contain an extremely low content of cardiolipin, a decreased level of phosphatidylcholine and an increased level of phosphatidylinositol. Disruption of the PEL1 gene in cells containing a null mutation in the CHO1 gene was lethal. Despite its putative ...

journal_title：FEMS microbiology letters

authors： Janitor M,Obernauerová M,Kohlwein SD,Subík J

更新日期：1996-06-15 00:00:00

abstract：:Carboxydothermus hydrogenoformans is an extremely thermophilic, Gram-positive bacterium growing on carbon monoxide (CO) as single carbon and energy source and producing only H(2) and CO(2). Carbon monoxide dehydrogenase is a key enzyme for CO metabolism. The carbon monoxide dehydrogenase genes cooF and cooS from C. hy...

journal_title：FEMS microbiology letters

authors： González JM,Robb FT

更新日期：2000-10-15 00:00:00